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Protein

24-hydroxycholesterol 7-alpha-hydroxylase

Gene

Cyp39a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.1 Publication

Catalytic activityi

(24S)-cholest-5-ene-3-beta,24-diol + [reduced NADPH--hemoprotein reductase] + O2 = (24S)-cholest-5-ene-3-beta,7-alpha,24-triol + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi415Iron (heme axial ligand)Sequence analysis1

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • oxysterol 7-alpha-hydroxylase activity Source: UniProtKB
  • steroid 7-alpha-hydroxylase activity Source: MGI

GO - Biological processi

  • bile acid biosynthetic process Source: UniProtKB
  • bile acid catabolic process Source: UniProtKB-KW
  • cholesterol catabolic process Source: MGI
  • digestion Source: UniProtKB

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processBile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.13.99 3474
ReactomeiR-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-211976 Endogenous sterols

Chemistry databases

SwissLipidsiSLP:000001228

Names & Taxonomyi

Protein namesi
Recommended name:
24-hydroxycholesterol 7-alpha-hydroxylase (EC:1.14.14.261 Publication)
Alternative name(s):
Cytochrome P450 39A1
Short name:
mCYP39A1
Oxysterol 7-alpha-hydroxylase
Gene namesi
Name:Cyp39a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927096 Cyp39a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519931 – 47024-hydroxycholesterol 7-alpha-hydroxylaseAdd BLAST470

Proteomic databases

MaxQBiQ9JKJ9
PaxDbiQ9JKJ9
PRIDEiQ9JKJ9

PTM databases

iPTMnetiQ9JKJ9
PhosphoSitePlusiQ9JKJ9
SwissPalmiQ9JKJ9

Expressioni

Tissue specificityi

Liver specific. Hepatic expression is sexually dimorphic (female > male).

Gene expression databases

BgeeiENSMUSG00000023963
ExpressionAtlasiQ9JKJ9 baseline and differential
GenevisibleiQ9JKJ9 MM

Interactioni

Protein-protein interaction databases

IntActiQ9JKJ9, 1 interactor
MINTiQ9JKJ9
STRINGi10090.ENSMUSP00000130073

Structurei

3D structure databases

ProteinModelPortaliQ9JKJ9
SMRiQ9JKJ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00550000074551
HOGENOMiHOG000290686
HOVERGENiHBG106232
InParanoidiQ9JKJ9
KOiK07439
OMAiIKWCILE
OrthoDBiEOG091G07L3
PhylomeDBiQ9JKJ9
TreeFamiTF105090

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PIRSFiPIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PRINTSiPR00465 EP450IV
SUPFAMiSSF48264 SSF48264, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JKJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIMELFSPI AIAVLGSCVL FLFSRLKNLL GPPCIQGWIP WIGAGLEFGK
60 70 80 90 100
APLEFIEKAR IKYGPVFTIF AMGNRMTFVS EEEGINVLLK SEHVDFESAV
110 120 130 140 150
QSPVYHTAWI PKNVFSALHE RLYALMKGKM GTFNTHHFTG PLTEELHEQL
160 170 180 190 200
EGLGTHGTMD LNDFVRYLLY PATLNTLFKK GLFLTDKRTI KEFYQQFKTY
210 220 230 240 250
DEGFEYGSQL PEWLLRNWSK SKRWLLALFE KNIGNIKAHG SAGHSGTLLQ
260 270 280 290 300
AILEVVETET RQYSPNYGLV VLWAALANAP PIAFWTLGYI LSHPDIHRTV
310 320 330 340 350
LESISSVFGT AGKDKIKVSE DDLKKLLIIK WCILESVRLR APGVITRKVV
360 370 380 390 400
KPVKILNHTV PSGDLLMLSP FWLHRNPKYF PEPESFKPER WKEANLDKYI
410 420 430 440 450
FLDYFMAFGG GKFQCPGRWF ALLEIQLCII LVLYKYECSL LDPLPKQSSR
460 470
HLVGVPQPAG KCRIEYKQRA
Length:470
Mass (Da):53,575
Last modified:October 1, 2000 - v1
Checksum:i9F08D3D936C26270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237981 mRNA Translation: AAF63328.1
CCDSiCCDS28799.1
RefSeqiNP_061375.1, NM_018887.4
UniGeneiMm.17991
Mm.376968

Genome annotation databases

EnsembliENSMUST00000170988; ENSMUSP00000130073; ENSMUSG00000023963
GeneIDi56050
KEGGimmu:56050
UCSCiuc008cpn.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCP39A_MOUSE
AccessioniPrimary (citable) accession number: Q9JKJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health