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Protein

24-hydroxycholesterol 7-alpha-hydroxylase

Gene

Cyp39a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.1 Publication

Catalytic activityi

(24S)-cholest-5-ene-3-beta,24-diol + [reduced NADPH--hemoprotein reductase] + O2 = (24S)-cholest-5-ene-3-beta,7-alpha,24-triol + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi415Iron (heme axial ligand)Sequence analysis1

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • oxysterol 7-alpha-hydroxylase activity Source: UniProtKB
  • steroid 7-alpha-hydroxylase activity Source: MGI

GO - Biological processi

  • bile acid biosynthetic process Source: UniProtKB
  • bile acid catabolic process Source: UniProtKB-KW
  • cholesterol catabolic process Source: MGI
  • digestion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.99. 3474.
ReactomeiR-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-211976. Endogenous sterols.

Chemistry databases

SwissLipidsiSLP:000001228.

Names & Taxonomyi

Protein namesi
Recommended name:
24-hydroxycholesterol 7-alpha-hydroxylase (EC:1.14.14.261 Publication)
Alternative name(s):
Cytochrome P450 39A1
Short name:
mCYP39A1
Oxysterol 7-alpha-hydroxylase
Gene namesi
Name:Cyp39a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927096. Cyp39a1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: UniProtKB
  • organelle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519931 – 47024-hydroxycholesterol 7-alpha-hydroxylaseAdd BLAST470

Proteomic databases

MaxQBiQ9JKJ9.
PaxDbiQ9JKJ9.
PRIDEiQ9JKJ9.

PTM databases

iPTMnetiQ9JKJ9.
PhosphoSitePlusiQ9JKJ9.

Expressioni

Tissue specificityi

Liver specific. Hepatic expression is sexually dimorphic (female > male).

Gene expression databases

BgeeiENSMUSG00000023963.
ExpressionAtlasiQ9JKJ9. baseline and differential.
GenevisibleiQ9JKJ9. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9JKJ9. 1 interactor.
MINTiMINT-4089498.
STRINGi10090.ENSMUSP00000130073.

Structurei

3D structure databases

ProteinModelPortaliQ9JKJ9.
SMRiQ9JKJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000290686.
HOVERGENiHBG106232.
InParanoidiQ9JKJ9.
KOiK07439.
OMAiYGLLMLW.
OrthoDBiEOG091G07L3.
PhylomeDBiQ9JKJ9.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JKJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIMELFSPI AIAVLGSCVL FLFSRLKNLL GPPCIQGWIP WIGAGLEFGK
60 70 80 90 100
APLEFIEKAR IKYGPVFTIF AMGNRMTFVS EEEGINVLLK SEHVDFESAV
110 120 130 140 150
QSPVYHTAWI PKNVFSALHE RLYALMKGKM GTFNTHHFTG PLTEELHEQL
160 170 180 190 200
EGLGTHGTMD LNDFVRYLLY PATLNTLFKK GLFLTDKRTI KEFYQQFKTY
210 220 230 240 250
DEGFEYGSQL PEWLLRNWSK SKRWLLALFE KNIGNIKAHG SAGHSGTLLQ
260 270 280 290 300
AILEVVETET RQYSPNYGLV VLWAALANAP PIAFWTLGYI LSHPDIHRTV
310 320 330 340 350
LESISSVFGT AGKDKIKVSE DDLKKLLIIK WCILESVRLR APGVITRKVV
360 370 380 390 400
KPVKILNHTV PSGDLLMLSP FWLHRNPKYF PEPESFKPER WKEANLDKYI
410 420 430 440 450
FLDYFMAFGG GKFQCPGRWF ALLEIQLCII LVLYKYECSL LDPLPKQSSR
460 470
HLVGVPQPAG KCRIEYKQRA
Length:470
Mass (Da):53,575
Last modified:October 1, 2000 - v1
Checksum:i9F08D3D936C26270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237981 mRNA. Translation: AAF63328.1.
CCDSiCCDS28799.1.
RefSeqiNP_061375.1. NM_018887.4.
UniGeneiMm.17991.
Mm.376968.

Genome annotation databases

EnsembliENSMUST00000170988; ENSMUSP00000130073; ENSMUSG00000023963.
GeneIDi56050.
KEGGimmu:56050.
UCSCiuc008cpn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237981 mRNA. Translation: AAF63328.1.
CCDSiCCDS28799.1.
RefSeqiNP_061375.1. NM_018887.4.
UniGeneiMm.17991.
Mm.376968.

3D structure databases

ProteinModelPortaliQ9JKJ9.
SMRiQ9JKJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JKJ9. 1 interactor.
MINTiMINT-4089498.
STRINGi10090.ENSMUSP00000130073.

Chemistry databases

SwissLipidsiSLP:000001228.

PTM databases

iPTMnetiQ9JKJ9.
PhosphoSitePlusiQ9JKJ9.

Proteomic databases

MaxQBiQ9JKJ9.
PaxDbiQ9JKJ9.
PRIDEiQ9JKJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170988; ENSMUSP00000130073; ENSMUSG00000023963.
GeneIDi56050.
KEGGimmu:56050.
UCSCiuc008cpn.2. mouse.

Organism-specific databases

CTDi51302.
MGIiMGI:1927096. Cyp39a1.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000290686.
HOVERGENiHBG106232.
InParanoidiQ9JKJ9.
KOiK07439.
OMAiYGLLMLW.
OrthoDBiEOG091G07L3.
PhylomeDBiQ9JKJ9.
TreeFamiTF105090.

Enzyme and pathway databases

BRENDAi1.14.13.99. 3474.
ReactomeiR-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-211976. Endogenous sterols.

Miscellaneous databases

PROiQ9JKJ9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023963.
ExpressionAtlasiQ9JKJ9. baseline and differential.
GenevisibleiQ9JKJ9. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCP39A_MOUSE
AccessioniPrimary (citable) accession number: Q9JKJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.