ID   CLC6A_MOUSE             Reviewed;         209 AA.
AC   Q9JKF4; Q9JKF2; Q9JKF3;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   21-SEP-2011, entry version 84.
DE   RecName: Full=C-type lectin domain family 6 member A;
DE   AltName: Full=C-type lectin superfamily member 10;
DE   AltName: Full=Dendritic cell-associated C-type lectin 2;
DE            Short=DC-associated C-type lectin 2;
DE            Short=Dectin-2;
GN   Name=Clec6a; Synonyms=Clec4n, Clecsf10, Dectin2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=BALB/c;
RX   PubMed=10766825; DOI=10.1074/jbc.275.16.11957;
RA   Ariizumi K., Shen G.-L., Shikano S., Ritter R. III, Zukas P.,
RA   Edelbaum D., Morita A., Takashima A.;
RT   "Cloning of a second dendritic cell-associated C-type lectin (dectin-
RT   2) and its alternatively spliced isoforms.";
RL   J. Biol. Chem. 275:11957-11963(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION, INTERACTION WITH FCER1G, AND MUTAGENESIS OF ARG-17.
RX   PubMed=17050534; DOI=10.1074/jbc.M606542200;
RA   Sato K., Yang X.L., Yudate T., Chung J.S., Wu J., Luby-Phelps K.,
RA   Kimberly R.P., Underhill D., Cruz P.D. Jr., Ariizumi K.;
RT   "Dectin-2 is a pattern recognition receptor for fungi that couples
RT   with the Fc receptor gamma chain to induce innate immune responses.";
RL   J. Biol. Chem. 281:38854-38866(2006).
RN   [4]
RP   FUNCTION.
RX   PubMed=19703985; DOI=10.1084/jem.20082818;
RA   Robinson M.J., Osorio F., Rosas M., Freitas R.P., Schweighoffer E.,
RA   Gross O., Verbeek J.S., Ruland J., Tybulewicz V., Brown G.D.,
RA   Moita L.F., Taylor P.R., Reis e Sousa C.;
RT   "Dectin-2 is a Syk-coupled pattern recognition receptor crucial for
RT   Th17 responses to fungal infection.";
RL   J. Exp. Med. 206:2037-2051(2009).
RN   [5]
RP   FUNCTION.
RX   PubMed=19124755;
RA   Barrett N.A., Maekawa A., Rahman O.M., Austen K.F., Kanaoka Y.;
RT   "Dectin-2 recognition of house dust mite triggers cysteinyl
RT   leukotriene generation by dendritic cells.";
RL   J. Immunol. 182:1119-1128(2009).
RN   [6]
RP   DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=20493731; DOI=10.1016/j.immuni.2010.05.001;
RA   Saijo S., Ikeda S., Yamabe K., Kakuta S., Ishigame H., Akitsu A.,
RA   Fujikado N., Kusaka T., Kubo S., Chung S.H., Komatsu R., Miura N.,
RA   Adachi Y., Ohno N., Shibuya K., Yamamoto N., Kawakami K., Yamasaki S.,
RA   Saito T., Akira S., Iwakura Y.;
RT   "Dectin-2 recognition of alpha-mannans and induction of Th17 cell
RT   differentiation is essential for host defense against Candida
RT   albicans.";
RL   Immunity 32:681-691(2010).
RN   [7]
RP   FUNCTION.
RX   PubMed=21059925; DOI=10.1073/pnas.1010337107;
RA   Ritter M., Gross O., Kays S., Ruland J., Nimmerjahn F., Saijo S.,
RA   Tschopp J., Layland L.E., Prazeres da Costa C.;
RT   "Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3
RT   inflammasome and alters adaptive immune responses.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:20459-20464(2010).
CC   -!- FUNCTION: Binds high-mannose carbohydrates in a Ca(2+)-dependent
CC       manner. Functionnal receptor for alpha-mannans on C. albicans
CC       hypheas. Plays an important role in the host defense against C.
CC       albicans infection by inducing TH17 cell differentiation.
CC       Recognizes also, in a manose dependent manner, allergens from
CC       house dust mite and fungi, by promoting cysteinyl leukotriene
CC       production. Recognizes soluble elements from the eggs of
CC       Shistosoma mansoni altering adaptative immune responses.
CC       Transduces signals through an Fc receptor gamma chain /FCER1G and
CC       Syk-CARD9-NF-kappa-B-dependent pathway.
CC   -!- SUBUNIT: Associated with FCER1G.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane
CC       protein (Probable). Note=Ligand binding leads to internalization.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1; Synonyms=Alpha;
CC         IsoId=Q9JKF4-1; Sequence=Displayed;
CC       Name=2; Synonyms=Beta;
CC         IsoId=Q9JKF4-2; Sequence=VSP_012846;
CC       Name=3; Synonyms=Gamma;
CC         IsoId=Q9JKF4-3; Sequence=VSP_012847;
CC   -!- TISSUE SPECIFICITY: Expressed by the XS52 DC (dendritic cell) line
CC       (at protein level). Expressed constitutively by the epidermis, and
CC       skin resident DC appear to be the major source of this expression.
CC       Expressed in the spleen and thymus. Expression was undetectable in
CC       non-DC lines, including macrophage lines (J774 and Raw), T-cell
CC       lines (7-17, HDK-1, and D10), B-cell hybridoma (5C5), a
CC       keratinocyte line (Pam 212), and a fibroblast line (NS01).
CC   -!- DOMAIN: A short stretch of the intracellular domain (AA 8-14)
CC       proximal to the transmembrane domain is required for association
CC       with Fc receptor gamma chain.
CC   -!- DISRUPTION PHENOTYPE: Deficient mice are healthy, fertile, with
CC       normal lymphoid cells, but show reduced survival after intravenous
CC       Candida albicans infection. In deficient mice fungal burden is
CC       higher in kidneys of mutant and in response to yeast antigen
CC       inflammatory cytokines and Th17 cells are reduced.
CC   -!- SIMILARITY: Contains 1 C-type lectin domain.
CC   -!- WEB RESOURCE: Name=Functional Glycomics Gateway - Glycan Binding;
CC       Note=Dectin-2;
CC       URL="http://www.functionalglycomics.org/glycomics/GBPServlet?&operationType=view&cbpId=cbp_mou_Ctlect_361";
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DR   EMBL; AF240357; AAF67177.1; -; mRNA.
DR   EMBL; AF240358; AAF67178.1; -; mRNA.
DR   EMBL; AF240359; AAF67179.1; -; mRNA.
DR   EMBL; BC023008; AAH23008.1; -; mRNA.
DR   IPI; IPI00122937; -.
DR   IPI; IPI00122939; -.
DR   IPI; IPI00459254; -.
DR   RefSeq; NP_001177250.1; NM_001190321.1.
DR   RefSeq; NP_064385.1; NM_020001.2.
DR   UniGene; Mm.271782; -.
DR   ProteinModelPortal; Q9JKF4; -.
DR   SMR; Q9JKF4; 73-206.
DR   STRING; Q9JKF4; -.
DR   PRIDE; Q9JKF4; -.
DR   Ensembl; ENSMUST00000024118; ENSMUSP00000024118; ENSMUSG00000023349.
DR   Ensembl; ENSMUST00000112554; ENSMUSP00000108173; ENSMUSG00000023349.
DR   GeneID; 56620; -.
DR   KEGG; mmu:56620; -.
DR   UCSC; uc009dqf.2; mouse.
DR   CTD; 56620; -.
DR   MGI; MGI:1861231; Clec4n.
DR   GeneTree; ENSGT00580000081462; -.
DR   HOGENOM; HBG283199; -.
DR   HOVERGEN; HBG050992; -.
DR   InParanoid; Q9JKF4; -.
DR   OMA; NDVFCDS; -.
DR   OrthoDB; EOG4HMJB7; -.
DR   PhylomeDB; Q9JKF4; -.
DR   NextBio; 313047; -.
DR   ArrayExpress; Q9JKF4; -.
DR   Bgee; Q9JKF4; -.
DR   CleanEx; MM_CLEC4N; -.
DR   Genevestigator; Q9JKF4; -.
DR   GermOnline; ENSMUSG00000023349; Mus musculus.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0005529; F:sugar binding; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR   GO; GO:0050715; P:positive regulation of cytokine secretion; IDA:UniProtKB.
DR   GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IDA:UniProtKB.
DR   InterPro; IPR002353; AntifreezeII.
DR   InterPro; IPR001304; C-type_lectin.
DR   InterPro; IPR016186; C-type_lectin-like.
DR   InterPro; IPR018378; C-type_lectin_CS.
DR   InterPro; IPR016187; C-type_lectin_fold.
DR   Gene3D; G3DSA:3.10.100.10; C-type_lectin-like; 1.
DR   Pfam; PF00059; Lectin_C; 1.
DR   PRINTS; PR00356; ANTIFREEZEII.
DR   SMART; SM00034; CLECT; 1.
DR   SUPFAM; SSF56436; C-type_lectin_fold; 1.
DR   PROSITE; PS00615; C_TYPE_LECTIN_1; 1.
DR   PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE   1: Evidence at protein level;
KW   Adaptive immunity; Alternative splicing; Complete proteome;
KW   Disulfide bond; Glycoprotein; Immunity; Innate immunity; Lectin;
KW   Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN         1    209       C-type lectin domain family 6 member A.
FT                                /FTId=PRO_0000046636.
FT   TOPO_DOM      1     20       Cytoplasmic (Potential).
FT   TRANSMEM     21     43       Helical; Signal-anchor for type II
FT                                membrane protein; (Potential).
FT   TOPO_DOM     44    209       Extracellular (Potential).
FT   DOMAIN       86    203       C-type lectin.
FT   CARBOHYD    131    131       N-linked (GlcNAc...) (Potential).
FT   DISULFID     79     90       By similarity.
FT   DISULFID    107    202       By similarity.
FT   DISULFID    176    194       By similarity.
FT   VAR_SEQ      39     72       Missing (in isoform 2).
FT                                /FTId=VSP_012846.
FT   VAR_SEQ     142    182       Missing (in isoform 3).
FT                                /FTId=VSP_012847.
FT   MUTAGEN      17     17       R->V: Does not affect association with Fc
FT                                receptor gamma chain.
SQ   SEQUENCE   209 AA;  24324 MW;  7AB8BDC0DB1EA5ED CRC64;
     MVQERQSQGK GVCWTLRLWS AAVISMLLLS TCFIASCVVT YQFIMDQPSR RLYELHTYHS
     SLTCFSEGTM VSEKMWGCCP NHWKSFGSSC YLISTKENFW STSEQNCVQM GAHLVVINTE
     AEQNFITQQL NESLSYFLGL SDPQGNGKWQ WIDDTPFSQN VRFWHPHEPN LPEERCVSIV
     YWNPSKWGWN DVFCDSKHNS ICEMKKIYL
//