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Q9JKF4 (CLC6A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 16, 2012. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-type lectin domain family 6 member A
Alternative name(s):
C-type lectin superfamily member 10
Dendritic cell-associated C-type lectin 2
Short name=DC-associated C-type lectin 2
Short name=Dectin-2
Gene names
Name:Clec6a
Synonyms:Clec4n, Clecsf10, Dectin2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds high-mannose carbohydrates in a Ca2+-dependent manner. Functionnal receptor for alpha-mannans on C. albicans hypheas. Plays an important role in the host defense against C. albicans infection by inducing TH17 cell differentiation. Recognizes also, in a manose dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptative immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Associated with FCER1G.

Subcellular location

Membrane; Single-pass type II membrane protein Probable. Note: Ligand binding leads to internalization.

Tissue specificity

Expressed by the XS52 DC (dendritic cell) line (at protein level). Expressed constitutively by the epidermis, and skin resident DC appear to be the major source of this expression. Expressed in the spleen and thymus. Expression was undetectable in non-DC lines, including macrophage lines (J774 and Raw), T-cell lines (7-17, HDK-1, and D10), B-cell hybridoma (5C5), a keratinocyte line (Pam 212), and a fibroblast line (NS01). Ref.1

Domain

A short stretch of the intracellular domain (AA 8-14) proximal to the transmembrane domain is required for association with Fc receptor gamma chain.

Disruption phenotype

Deficient mice are healthy, fertile, with normal lymphoid cells, but show reduced survival after intravenous Candida albicans infection. In deficient mice fungal burden is higher in kidneys of mutant and in response to yeast antigen inflammatory cytokines and Th17 cells are reduced. Ref.6

Sequence similarities

Contains 1 C-type lectin domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JKF4-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JKF4-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     39-72: Missing.
Isoform 3 (identifier: Q9JKF4-3)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     142-182: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209C-type lectin domain family 6 member A
PRO_0000046636

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4323Helical; Signal-anchor for type II membrane protein; Potential
Topological domain44 – 209166Extracellular Potential
Domain86 – 203118C-type lectin

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential
Disulfide bond79 ↔ 90 By similarity
Disulfide bond107 ↔ 202 By similarity
Disulfide bond176 ↔ 194 By similarity

Natural variations

Alternative sequence39 – 7234Missing in isoform 2.
VSP_012846
Alternative sequence142 – 18241Missing in isoform 3.
VSP_012847

Experimental info

Mutagenesis171R → V: Does not affect association with Fc receptor gamma chain. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 7AB8BDC0DB1EA5ED

FASTA20924,324
        10         20         30         40         50         60 
MVQERQSQGK GVCWTLRLWS AAVISMLLLS TCFIASCVVT YQFIMDQPSR RLYELHTYHS 

        70         80         90        100        110        120 
SLTCFSEGTM VSEKMWGCCP NHWKSFGSSC YLISTKENFW STSEQNCVQM GAHLVVINTE 

       130        140        150        160        170        180 
AEQNFITQQL NESLSYFLGL SDPQGNGKWQ WIDDTPFSQN VRFWHPHEPN LPEERCVSIV 

       190        200 
YWNPSKWGWN DVFCDSKHNS ICEMKKIYL

« Hide

Isoform 2 (Beta) [UniParc].

Checksum: E1C9632687BF0EA9
Show »

FASTA17520,313
Isoform 3 (Gamma) [UniParc].

Checksum: CF2ADDFE054EB038
Show »

FASTA16819,332

References

« Hide 'large scale' references
[1]"Cloning of a second dendritic cell-associated C-type lectin (dectin-2) and its alternatively spliced isoforms."
Ariizumi K., Shen G.-L., Shikano S., Ritter R. III, Zukas P., Edelbaum D., Morita A., Takashima A.
J. Biol. Chem. 275:11957-11963(2000) [PubMed: 10766825] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
Strain: BALB/c.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N-3.
Tissue: Mammary tumor.
[3]"Dectin-2 is a pattern recognition receptor for fungi that couples with the Fc receptor gamma chain to induce innate immune responses."
Sato K., Yang X.L., Yudate T., Chung J.S., Wu J., Luby-Phelps K., Kimberly R.P., Underhill D., Cruz P.D. Jr., Ariizumi K.
J. Biol. Chem. 281:38854-38866(2006) [PubMed: 17050534] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FCER1G, MUTAGENESIS OF ARG-17.
[4]"Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection."
Robinson M.J., Osorio F., Rosas M., Freitas R.P., Schweighoffer E., Gross O., Verbeek J.S., Ruland J., Tybulewicz V., Brown G.D., Moita L.F., Taylor P.R., Reis e Sousa C.
J. Exp. Med. 206:2037-2051(2009) [PubMed: 19703985] [Abstract]
Cited for: FUNCTION.
[5]"Dectin-2 recognition of house dust mite triggers cysteinyl leukotriene generation by dendritic cells."
Barrett N.A., Maekawa A., Rahman O.M., Austen K.F., Kanaoka Y.
J. Immunol. 182:1119-1128(2009) [PubMed: 19124755] [Abstract]
Cited for: FUNCTION.
[6]"Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans."
Saijo S., Ikeda S., Yamabe K., Kakuta S., Ishigame H., Akitsu A., Fujikado N., Kusaka T., Kubo S., Chung S.H., Komatsu R., Miura N., Adachi Y., Ohno N., Shibuya K., Yamamoto N., Kawakami K., Yamasaki S. expand/collapse author list , Saito T., Akira S., Iwakura Y.
Immunity 32:681-691(2010) [PubMed: 20493731] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[7]"Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses."
Ritter M., Gross O., Kays S., Ruland J., Nimmerjahn F., Saijo S., Tschopp J., Layland L.E., Prazeres da Costa C.
Proc. Natl. Acad. Sci. U.S.A. 107:20459-20464(2010) [PubMed: 21059925] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF240357 mRNA. Translation: AAF67177.1.
AF240358 mRNA. Translation: AAF67178.1.
AF240359 mRNA. Translation: AAF67179.1.
BC023008 mRNA. Translation: AAH23008.1.
IPIIPI00122937.
IPI00122939.
IPI00459254.
RefSeqNP_001177250.1. NM_001190321.1.
NP_064385.1. NM_020001.2.
UniGeneMm.271782.

3D structure databases

ProteinModelPortalQ9JKF4.
SMRQ9JKF4. Positions 73-206.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9JKF4.

Proteomic databases

PRIDEQ9JKF4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024118; ENSMUSP00000024118; ENSMUSG00000023349.
ENSMUST00000112554; ENSMUSP00000108173; ENSMUSG00000023349.
GeneID56620.
KEGGmmu:56620.
UCSCuc009dqf.2. mouse.

Organism-specific databases

CTD56620.
MGIMGI:1861231. Clec4n.

Phylogenomic databases

eggNOGNOG263776.
GeneTreeENSGT00650000092998.
HOGENOMHOG000059556.
HOVERGENHBG050992.
InParanoidQ9JKF4.
OMANDVFCDS.
OrthoDBEOG4HMJB7.

Gene expression databases

ArrayExpressQ9JKF4.
BgeeQ9JKF4.
CleanExMM_CLEC4N.
GenevestigatorQ9JKF4.
GermOnlineENSMUSG00000023349. Mus musculus.

Family and domain databases

Gene3DG3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
InterProIPR002353. AntifreezeII.
IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
[Graphical view]
PRINTSPR00356. ANTIFREEZEII.
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. C-type_lectin_fold. 1 hit.
PROSITEPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio313047.
SOURCESearch...

Entry information

Entry nameCLC6A_MOUSE
AccessionPrimary (citable) accession number: Q9JKF4
Secondary accession number(s): Q9JKF2, Q9JKF3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 1, 2000
Last modified: May 16, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents