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Protein

Ras GTPase-activating-like protein IQGAP1

Gene

Iqgap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

GO - Molecular functioni

GO - Biological processi

  • cellular response to calcium ion Source: MGI
  • cellular response to epidermal growth factor stimulus Source: BHF-UCL
  • cellular response to fibroblast growth factor stimulus Source: BHF-UCL
  • cellular response to platelet-derived growth factor stimulus Source: BHF-UCL
  • epidermal growth factor receptor signaling pathway Source: BHF-UCL
  • fibroblast growth factor receptor signaling pathway Source: BHF-UCL
  • glomerular visceral epithelial cell development Source: UniProtKB
  • negative regulation of dephosphorylation Source: MGI
  • neuron projection extension Source: MGI
  • platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
  • positive regulation of cellular protein localization Source: Ensembl
  • positive regulation of dendrite development Source: Ensembl
  • positive regulation of focal adhesion assembly Source: Ensembl
  • positive regulation of MAP kinase activity Source: Ensembl
  • positive regulation of peptidyl-tyrosine autophosphorylation Source: Ensembl
  • positive regulation of protein kinase activity Source: BHF-UCL
  • positive regulation of vascular associated smooth muscle cell migration Source: Ensembl
  • regulation of cytokine production Source: MGI
  • regulation of GTPase activity Source: InterPro
  • response to angiotensin Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-373753. Nephrin interactions.
R-MMU-5626467. RHO GTPases activate IQGAPs.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating-like protein IQGAP1
Gene namesi
Name:Iqgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1352757. Iqgap1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: Ensembl
  • axon Source: UniProtKB
  • cell-cell adherens junction Source: MGI
  • cell-cell junction Source: MGI
  • cell leading edge Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic ribonucleoprotein granule Source: MGI
  • extracellular exosome Source: MGI
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • focal adhesion Source: MGI
  • growth cone Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: MGI
  • lateral plasma membrane Source: MGI
  • membrane Source: MGI
  • membrane raft Source: Ensembl
  • microtubule Source: MGI
  • midbody Source: MGI
  • neuron projection Source: UniProtKB
  • nucleus Source: Ensembl
  • protein complex Source: Ensembl
  • ruffle Source: MGI
  • slit diaphragm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000566492 – 1657Ras GTPase-activating-like protein IQGAP1Add BLAST1656

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei172PhosphotyrosineCombined sources1
Modified residuei330PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JKF1.
MaxQBiQ9JKF1.
PaxDbiQ9JKF1.
PeptideAtlasiQ9JKF1.
PRIDEiQ9JKF1.

PTM databases

iPTMnetiQ9JKF1.
PhosphoSitePlusiQ9JKF1.
SwissPalmiQ9JKF1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030536.
CleanExiMM_IQGAP1.
ExpressionAtlasiQ9JKF1. baseline and differential.
GenevisibleiQ9JKF1. MM.

Interactioni

Subunit structurei

Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6 (By similarity). In case of infection, interacts with S.typhimurium protein sseI.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gagP0333217EBI-644633,EBI-935477From a different organism.
Shc1P980835EBI-644633,EBI-300201

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205938. 9 interactors.
IntActiQ9JKF1. 11 interactors.
MINTiMINT-1563339.
STRINGi10090.ENSMUSP00000128278.

Structurei

3D structure databases

ProteinModelPortaliQ9JKF1.
SMRiQ9JKF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 159CHPROSITE-ProRule annotationAdd BLAST116
Domaini685 – 710WWAdd BLAST26
Domaini745 – 774IQ 1PROSITE-ProRule annotationAdd BLAST30
Domaini775 – 804IQ 2PROSITE-ProRule annotationAdd BLAST30
Domaini805 – 834IQ 3PROSITE-ProRule annotationAdd BLAST30
Domaini835 – 864IQ 4PROSITE-ProRule annotationAdd BLAST30
Domaini1004 – 1237Ras-GAPPROSITE-ProRule annotationAdd BLAST234

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni956 – 1274C1Add BLAST319
Regioni1276 – 1657C2Add BLAST382

Domaini

Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together.By similarity

Sequence similaritiesi

Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 4 IQ domains.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation
Contains 1 WW domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2128. Eukaryota.
COG5261. LUCA.
GeneTreeiENSGT00600000084379.
HOVERGENiHBG052143.
InParanoidiQ9JKF1.
KOiK16848.
OMAiIPECGET.
OrthoDBiEOG091G00ET.
TreeFamiTF313078.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
1.10.506.10. 2 hits.
4.10.270.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR027417. P-loop_NTPase.
IPR000593. RasGAP_C.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00612. IQ. 4 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00015. IQ. 4 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS50096. IQ. 4 hits.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JKF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAEEVDGL GVVRPHYGSV LDNERLTAEE MDERRRQNVA YEYLCHLEEA
60 70 80 90 100
KRWMEACLGE DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ
110 120 130 140 150
TRYKATGLHF RHTDNVIQWL NAMDEIGLPK IFYPETTDIY DRKNMPRCIY
160 170 180 190 200
CIHALSLYLF KLGLAPQIQD LYGKVDFTEE EINNMKIELE KYGIQMPAFS
210 220 230 240 250
KIGGILANEL SVDEAALHAA VIAINEAIDR RVAADTFTAL KNPNAMLVNL
260 270 280 290 300
EEGLAPTYQD VLYQAKQDKM TNAKNRTENS DRERDVYEEL LTQAEIQGNV
310 320 330 340 350
NKVNTSSALA NISLALEQGC AVTLLKALQS LALGLRGLQT QNSDWYMKQL
360 370 380 390 400
QSDLQQKRQS GQTDPLQKEE VQAGVDAANS AAQQYQRRLA AVAAINAAIQ
410 420 430 440 450
KGIAEKTVLE LMNPEAQLPQ VYPFAADLYQ KELATLQQQS PEHSLTHPEL
460 470 480 490 500
TVAVEMLSSV ALINRALESG DMTTVWKQLS SSVTGLTNIE EENCQRYLDE
510 520 530 540 550
LMKLKAQAHA ENNAFITWND IQACVDHVNL VVHEEHERIL AIGLINEALD
560 570 580 590 600
EGDAQKTLQA LQIPAAKLEG VLAEVAQHYQ DTLIRAKREK AQETQDESAV
610 620 630 640 650
LWLDEIQGGI WQSNKDTQEA QRFALGISAI NEAVDSGDVG RTLSALRSPD
660 670 680 690 700
VGLYGVIPEC GETYQSDLAE AKKKRLAAGD NNSKWVKHWV KGGYHYYHNL
710 720 730 740 750
ETQAGGWAEP PDFVQNSVQL SREEIQSSIS GVTAAYNREQ LWLANEGLIT
760 770 780 790 800
KLQACCRGYL VRQEFRSRMN FLKKQIPAIT CIQSQWRGYK QKKAYQDRLA
810 820 830 840 850
YLHSHKDEVV KIQSLARMHQ ARKRYRDRLQ YFRDHINDII KIQAFIRANK
860 870 880 890 900
ARDDYKTLIN AEDPPMIVVR KFVHLLDQSD QDFQEELDLM KMREEVITLI
910 920 930 940 950
RSNQQLENDL NLMDIKIGLL VKNKITLQDV VSHSKKLTKK NKEQLSDMMM
960 970 980 990 1000
INKQKGGLKA LSKEKREKLE AYQHLFYLLQ TNPTYLAKLI FQMPQNKSTK
1010 1020 1030 1040 1050
FMDSVIFTLY NYASNQREEY LLLRLFQTAL QEEIKSKVDQ IQEIVTGNPT
1060 1070 1080 1090 1100
VIKMVVSFNR GARGQNALRQ ILAPVVKEIM DDKSLNIKTD PVDIYKSWVN
1110 1120 1130 1140 1150
QMESQTGEAS KLPYDVTPEQ ALSHEEVKTR LDNSIRNMRA VTDKFLSAIV
1160 1170 1180 1190 1200
SSVDKIPYGM RFIAKVLKDS LHEKFPDAGE DELLKIIGNL LYYRYMNPAI
1210 1220 1230 1240 1250
VAPDAFDIID LSAGGQLTTD QRRNLGSIAK MLQHAASNKM FLGDNAHLSI
1260 1270 1280 1290 1300
INEYLSQSYQ KFRRFFQVAC DVPELQDKFN VDEYSDLVTL TKPVIYISIG
1310 1320 1330 1340 1350
EIINTHTLLL DHQDAIAPEH NDPIHELLDD LGEVPTIESL IGESCGNSND
1360 1370 1380 1390 1400
PNKEALAKTE VSLTLTNKFD VPGDENAEMD ARTILLNTKR LIVDVIRFQP
1410 1420 1430 1440 1450
GETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK SKPMKEDNNL
1460 1470 1480 1490 1500
SLQEKKEKIQ TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRK
1510 1520 1530 1540 1550
AELVKLQQTY SALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE
1560 1570 1580 1590 1600
MKGKKSKKIS LKYTAARLHE KGVLLEIEDL QANQFKNVIF EIGPTEEVGD
1610 1620 1630 1640 1650
FEVKAKFMGV QMETFMLHYQ DLLQLQYEGV AVMKLFDRAK VNVNLLIFLL

NKKFYGK
Length:1,657
Mass (Da):188,742
Last modified:October 3, 2012 - v2
Checksum:i9136695557ADA784
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1268V → L in AAF60344 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240630 mRNA. Translation: AAF60344.1.
AC109257 Genomic DNA. No translation available.
AC127594 Genomic DNA. No translation available.
CH466543 Genomic DNA. Translation: EDL06977.1.
CCDSiCCDS40001.1.
RefSeqiNP_057930.2. NM_016721.2.
UniGeneiMm.207619.

Genome annotation databases

EnsembliENSMUST00000167377; ENSMUSP00000128278; ENSMUSG00000030536.
GeneIDi29875.
KEGGimmu:29875.
UCSCiuc009ibc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240630 mRNA. Translation: AAF60344.1.
AC109257 Genomic DNA. No translation available.
AC127594 Genomic DNA. No translation available.
CH466543 Genomic DNA. Translation: EDL06977.1.
CCDSiCCDS40001.1.
RefSeqiNP_057930.2. NM_016721.2.
UniGeneiMm.207619.

3D structure databases

ProteinModelPortaliQ9JKF1.
SMRiQ9JKF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205938. 9 interactors.
IntActiQ9JKF1. 11 interactors.
MINTiMINT-1563339.
STRINGi10090.ENSMUSP00000128278.

PTM databases

iPTMnetiQ9JKF1.
PhosphoSitePlusiQ9JKF1.
SwissPalmiQ9JKF1.

Proteomic databases

EPDiQ9JKF1.
MaxQBiQ9JKF1.
PaxDbiQ9JKF1.
PeptideAtlasiQ9JKF1.
PRIDEiQ9JKF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000167377; ENSMUSP00000128278; ENSMUSG00000030536.
GeneIDi29875.
KEGGimmu:29875.
UCSCiuc009ibc.2. mouse.

Organism-specific databases

CTDi8826.
MGIiMGI:1352757. Iqgap1.

Phylogenomic databases

eggNOGiKOG2128. Eukaryota.
COG5261. LUCA.
GeneTreeiENSGT00600000084379.
HOVERGENiHBG052143.
InParanoidiQ9JKF1.
KOiK16848.
OMAiIPECGET.
OrthoDBiEOG091G00ET.
TreeFamiTF313078.

Enzyme and pathway databases

ReactomeiR-MMU-373753. Nephrin interactions.
R-MMU-5626467. RHO GTPases activate IQGAPs.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiIqgap1. mouse.
PROiQ9JKF1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030536.
CleanExiMM_IQGAP1.
ExpressionAtlasiQ9JKF1. baseline and differential.
GenevisibleiQ9JKF1. MM.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
1.10.506.10. 2 hits.
4.10.270.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR027417. P-loop_NTPase.
IPR000593. RasGAP_C.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00612. IQ. 4 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00015. IQ. 4 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS50096. IQ. 4 hits.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIQGA1_MOUSE
AccessioniPrimary (citable) accession number: Q9JKF1
Secondary accession number(s): G3UW45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.