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Q9JKF1

- IQGA1_MOUSE

UniProt

Q9JKF1 - IQGA1_MOUSE

Protein

Ras GTPase-activating-like protein IQGAP1

Gene

Iqgap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

    GO - Molecular functioni

    1. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. Rac GTPase binding Source: MGI
    4. Ras GTPase activator activity Source: InterPro
    5. Rho GTPase binding Source: MGI

    GO - Biological processi

    1. cellular response to calcium ion Source: Ensembl
    2. cellular response to epidermal growth factor stimulus Source: BHF-UCL
    3. cellular response to fibroblast growth factor stimulus Source: BHF-UCL
    4. cellular response to platelet-derived growth factor stimulus Source: BHF-UCL
    5. epidermal growth factor receptor signaling pathway Source: BHF-UCL
    6. fibroblast growth factor receptor signaling pathway Source: BHF-UCL
    7. glomerular visceral epithelial cell development Source: UniProtKB
    8. negative regulation of dephosphorylation Source: MGI
    9. platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
    10. positive regulation of protein kinase activity Source: BHF-UCL
    11. regulation of cytokine production Source: MGI
    12. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    Calmodulin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras GTPase-activating-like protein IQGAP1
    Gene namesi
    Name:Iqgap1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1352757. Iqgap1.

    Subcellular locationi

    Cell membrane By similarity

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. cell leading edge Source: MGI
    3. cytoplasm Source: MGI
    4. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
    5. lateral plasma membrane Source: MGI
    6. membrane Source: MGI
    7. microtubule Source: Ensembl
    8. midbody Source: Ensembl
    9. neuron projection Source: MGI
    10. nucleus Source: Ensembl
    11. ribonucleoprotein complex Source: MGI
    12. slit diaphragm Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 16571656Ras GTPase-activating-like protein IQGAP1PRO_0000056649Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei172 – 1721Phosphotyrosine1 Publication
    Modified residuei330 – 3301PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9JKF1.
    PaxDbiQ9JKF1.
    PRIDEiQ9JKF1.

    PTM databases

    PhosphoSiteiQ9JKF1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9JKF1.
    CleanExiMM_IQGAP1.
    GenevestigatoriQ9JKF1.

    Interactioni

    Subunit structurei

    Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6 By similarity. In case of infection, interacts with S.typhimurium protein sseI.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gagP0333217EBI-644633,EBI-935477From a different organism.
    Shc1P980835EBI-644633,EBI-300201

    Protein-protein interaction databases

    IntActiQ9JKF1. 9 interactions.
    MINTiMINT-1563339.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JKF1.
    SMRiQ9JKF1. Positions 26-210, 962-1339, 1561-1657.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 159116CHPROSITE-ProRule annotationAdd
    BLAST
    Domaini685 – 71026WWAdd
    BLAST
    Domaini745 – 77430IQ 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini775 – 80430IQ 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini805 – 83430IQ 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini835 – 86430IQ 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1004 – 1237234Ras-GAPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni956 – 1274319C1Add
    BLAST
    Regioni1276 – 1657382C2Add
    BLAST

    Domaini

    Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together.By similarity

    Sequence similaritiesi

    Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
    Contains 4 IQ domains.PROSITE-ProRule annotation
    Contains 1 Ras-GAP domain.PROSITE-ProRule annotation
    Contains 1 WW domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5261.
    GeneTreeiENSGT00600000084379.
    HOVERGENiHBG052143.
    InParanoidiQ9JKF1.
    KOiK16848.
    OMAiQDILYQA.
    OrthoDBiEOG7FNC6M.
    TreeFamiTF313078.

    Family and domain databases

    Gene3Di1.10.418.10. 1 hit.
    1.10.506.10. 2 hits.
    4.10.270.10. 1 hit.
    InterProiIPR001715. CH-domain.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR027417. P-loop_NTPase.
    IPR001936. RasGAP.
    IPR000593. RasGAP_C.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    IPR001202. WW_dom.
    [Graphical view]
    PfamiPF00307. CH. 1 hit.
    PF00612. IQ. 4 hits.
    PF00616. RasGAP. 1 hit.
    PF03836. RasGAP_C. 1 hit.
    [Graphical view]
    SMARTiSM00033. CH. 1 hit.
    SM00015. IQ. 4 hits.
    SM00323. RasGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    SSF48350. SSF48350. 2 hits.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50021. CH. 1 hit.
    PS50096. IQ. 4 hits.
    PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    PS01159. WW_DOMAIN_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9JKF1-1 [UniParc]FASTAAdd to Basket

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    MSAAEEVDGL GVVRPHYGSV LDNERLTAEE MDERRRQNVA YEYLCHLEEA     50
    KRWMEACLGE DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ 100
    TRYKATGLHF RHTDNVIQWL NAMDEIGLPK IFYPETTDIY DRKNMPRCIY 150
    CIHALSLYLF KLGLAPQIQD LYGKVDFTEE EINNMKIELE KYGIQMPAFS 200
    KIGGILANEL SVDEAALHAA VIAINEAIDR RVAADTFTAL KNPNAMLVNL 250
    EEGLAPTYQD VLYQAKQDKM TNAKNRTENS DRERDVYEEL LTQAEIQGNV 300
    NKVNTSSALA NISLALEQGC AVTLLKALQS LALGLRGLQT QNSDWYMKQL 350
    QSDLQQKRQS GQTDPLQKEE VQAGVDAANS AAQQYQRRLA AVAAINAAIQ 400
    KGIAEKTVLE LMNPEAQLPQ VYPFAADLYQ KELATLQQQS PEHSLTHPEL 450
    TVAVEMLSSV ALINRALESG DMTTVWKQLS SSVTGLTNIE EENCQRYLDE 500
    LMKLKAQAHA ENNAFITWND IQACVDHVNL VVHEEHERIL AIGLINEALD 550
    EGDAQKTLQA LQIPAAKLEG VLAEVAQHYQ DTLIRAKREK AQETQDESAV 600
    LWLDEIQGGI WQSNKDTQEA QRFALGISAI NEAVDSGDVG RTLSALRSPD 650
    VGLYGVIPEC GETYQSDLAE AKKKRLAAGD NNSKWVKHWV KGGYHYYHNL 700
    ETQAGGWAEP PDFVQNSVQL SREEIQSSIS GVTAAYNREQ LWLANEGLIT 750
    KLQACCRGYL VRQEFRSRMN FLKKQIPAIT CIQSQWRGYK QKKAYQDRLA 800
    YLHSHKDEVV KIQSLARMHQ ARKRYRDRLQ YFRDHINDII KIQAFIRANK 850
    ARDDYKTLIN AEDPPMIVVR KFVHLLDQSD QDFQEELDLM KMREEVITLI 900
    RSNQQLENDL NLMDIKIGLL VKNKITLQDV VSHSKKLTKK NKEQLSDMMM 950
    INKQKGGLKA LSKEKREKLE AYQHLFYLLQ TNPTYLAKLI FQMPQNKSTK 1000
    FMDSVIFTLY NYASNQREEY LLLRLFQTAL QEEIKSKVDQ IQEIVTGNPT 1050
    VIKMVVSFNR GARGQNALRQ ILAPVVKEIM DDKSLNIKTD PVDIYKSWVN 1100
    QMESQTGEAS KLPYDVTPEQ ALSHEEVKTR LDNSIRNMRA VTDKFLSAIV 1150
    SSVDKIPYGM RFIAKVLKDS LHEKFPDAGE DELLKIIGNL LYYRYMNPAI 1200
    VAPDAFDIID LSAGGQLTTD QRRNLGSIAK MLQHAASNKM FLGDNAHLSI 1250
    INEYLSQSYQ KFRRFFQVAC DVPELQDKFN VDEYSDLVTL TKPVIYISIG 1300
    EIINTHTLLL DHQDAIAPEH NDPIHELLDD LGEVPTIESL IGESCGNSND 1350
    PNKEALAKTE VSLTLTNKFD VPGDENAEMD ARTILLNTKR LIVDVIRFQP 1400
    GETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK SKPMKEDNNL 1450
    SLQEKKEKIQ TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRK 1500
    AELVKLQQTY SALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE 1550
    MKGKKSKKIS LKYTAARLHE KGVLLEIEDL QANQFKNVIF EIGPTEEVGD 1600
    FEVKAKFMGV QMETFMLHYQ DLLQLQYEGV AVMKLFDRAK VNVNLLIFLL 1650
    NKKFYGK 1657
    Length:1,657
    Mass (Da):188,742
    Last modified:October 3, 2012 - v2
    Checksum:i9136695557ADA784
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1268 – 12681V → L in AAF60344. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF240630 mRNA. Translation: AAF60344.1.
    AC109257 Genomic DNA. No translation available.
    AC127594 Genomic DNA. No translation available.
    CH466543 Genomic DNA. Translation: EDL06977.1.
    CCDSiCCDS40001.1.
    RefSeqiNP_057930.2. NM_016721.2.
    UniGeneiMm.207619.

    Genome annotation databases

    EnsembliENSMUST00000167377; ENSMUSP00000128278; ENSMUSG00000030536.
    GeneIDi29875.
    KEGGimmu:29875.
    UCSCiuc009ibc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF240630 mRNA. Translation: AAF60344.1 .
    AC109257 Genomic DNA. No translation available.
    AC127594 Genomic DNA. No translation available.
    CH466543 Genomic DNA. Translation: EDL06977.1 .
    CCDSi CCDS40001.1.
    RefSeqi NP_057930.2. NM_016721.2.
    UniGenei Mm.207619.

    3D structure databases

    ProteinModelPortali Q9JKF1.
    SMRi Q9JKF1. Positions 26-210, 962-1339, 1561-1657.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9JKF1. 9 interactions.
    MINTi MINT-1563339.

    PTM databases

    PhosphoSitei Q9JKF1.

    Proteomic databases

    MaxQBi Q9JKF1.
    PaxDbi Q9JKF1.
    PRIDEi Q9JKF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000167377 ; ENSMUSP00000128278 ; ENSMUSG00000030536 .
    GeneIDi 29875.
    KEGGi mmu:29875.
    UCSCi uc009ibc.2. mouse.

    Organism-specific databases

    CTDi 8826.
    MGIi MGI:1352757. Iqgap1.

    Phylogenomic databases

    eggNOGi COG5261.
    GeneTreei ENSGT00600000084379.
    HOVERGENi HBG052143.
    InParanoidi Q9JKF1.
    KOi K16848.
    OMAi QDILYQA.
    OrthoDBi EOG7FNC6M.
    TreeFami TF313078.

    Miscellaneous databases

    ChiTaRSi IQGAP1. mouse.
    NextBioi 307110.
    PROi Q9JKF1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JKF1.
    CleanExi MM_IQGAP1.
    Genevestigatori Q9JKF1.

    Family and domain databases

    Gene3Di 1.10.418.10. 1 hit.
    1.10.506.10. 2 hits.
    4.10.270.10. 1 hit.
    InterProi IPR001715. CH-domain.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR027417. P-loop_NTPase.
    IPR001936. RasGAP.
    IPR000593. RasGAP_C.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    IPR001202. WW_dom.
    [Graphical view ]
    Pfami PF00307. CH. 1 hit.
    PF00612. IQ. 4 hits.
    PF00616. RasGAP. 1 hit.
    PF03836. RasGAP_C. 1 hit.
    [Graphical view ]
    SMARTi SM00033. CH. 1 hit.
    SM00015. IQ. 4 hits.
    SM00323. RasGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    SSF48350. SSF48350. 2 hits.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50021. CH. 1 hit.
    PS50096. IQ. 4 hits.
    PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    PS01159. WW_DOMAIN_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of Mus musculus Cdc42 effector protein IQGAP1."
      Bernards A.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pre-B cell.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Identification of a DOCK180-related guanine nucleotide exchange factor that is capable of mediating a positive feedback activation of Cdc42."
      Lin Q., Yang W., Baird D., Feng Q., Cerione R.A.
      J. Biol. Chem. 281:35253-35262(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC42.
    6. "The Salmonella SPI2 effector SseI mediates long-term systemic infection by modulating host cell migration."
      McLaughlin L.M., Govoni G.R., Gerke C., Gopinath S., Peng K., Laidlaw G., Chien Y.H., Jeong H.W., Li Z., Brown M.D., Sacks D.B., Monack D.
      PLoS Pathog. 5:E1000671-E1000671(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH S.TYPHIMURIUM SSEI.

    Entry informationi

    Entry nameiIQGA1_MOUSE
    AccessioniPrimary (citable) accession number: Q9JKF1
    Secondary accession number(s): G3UW45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3