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Q9JKF1

- IQGA1_MOUSE

UniProt

Q9JKF1 - IQGA1_MOUSE

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Protein
Ras GTPase-activating-like protein IQGAP1
Gene
Iqgap1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

GO - Molecular functioni

  1. Rac GTPase binding Source: MGI
  2. Ras GTPase activator activity Source: InterPro
  3. Rho GTPase binding Source: MGI
  4. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  5. protein binding Source: IntAct

GO - Biological processi

  1. cellular response to calcium ion Source: Ensembl
  2. glomerular visceral epithelial cell development Source: UniProtKB
  3. negative regulation of dephosphorylation Source: MGI
  4. positive regulation of protein kinase activity Source: BHF-UCL
  5. regulation of cytokine production Source: MGI
  6. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating-like protein IQGAP1
Gene namesi
Name:Iqgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1352757. Iqgap1.

Subcellular locationi

Cell membrane By similarity

GO - Cellular componenti

  1. cell leading edge Source: MGI
  2. cell-cell junction Source: MGI
  3. cytoplasm Source: MGI
  4. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  5. lateral plasma membrane Source: MGI
  6. membrane Source: MGI
  7. microtubule Source: Ensembl
  8. midbody Source: Ensembl
  9. neuron projection Source: MGI
  10. nucleus Source: Ensembl
  11. ribonucleoprotein complex Source: MGI
  12. slit diaphragm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 16571656Ras GTPase-activating-like protein IQGAP1
PRO_0000056649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei2 – 21Phosphoserine By similarity
Modified residuei172 – 1721Phosphotyrosine1 Publication
Modified residuei330 – 3301Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JKF1.
PaxDbiQ9JKF1.
PRIDEiQ9JKF1.

PTM databases

PhosphoSiteiQ9JKF1.

Expressioni

Gene expression databases

ArrayExpressiQ9JKF1.
CleanExiMM_IQGAP1.
GenevestigatoriQ9JKF1.

Interactioni

Subunit structurei

Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6 By similarity. In case of infection, interacts with S.typhimurium protein sseI.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gagP0333217EBI-644633,EBI-935477From a different organism.
Shc1P980835EBI-644633,EBI-300201

Protein-protein interaction databases

IntActiQ9JKF1. 8 interactions.
MINTiMINT-1563339.

Structurei

3D structure databases

ProteinModelPortaliQ9JKF1.
SMRiQ9JKF1. Positions 26-210, 962-1339, 1561-1657.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 159116CH
Add
BLAST
Domaini685 – 71026WW
Add
BLAST
Domaini745 – 77430IQ 1
Add
BLAST
Domaini775 – 80430IQ 2
Add
BLAST
Domaini805 – 83430IQ 3
Add
BLAST
Domaini835 – 86430IQ 4
Add
BLAST
Domaini1004 – 1237234Ras-GAP
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni956 – 1274319C1
Add
BLAST
Regioni1276 – 1657382C2
Add
BLAST

Domaini

Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together By similarity.

Sequence similaritiesi

Contains 4 IQ domains.
Contains 1 Ras-GAP domain.
Contains 1 WW domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5261.
GeneTreeiENSGT00600000084379.
HOVERGENiHBG052143.
InParanoidiQ9JKF1.
KOiK16848.
OMAiQDILYQA.
OrthoDBiEOG7FNC6M.
TreeFamiTF313078.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
1.10.506.10. 2 hits.
4.10.270.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR027417. P-loop_NTPase.
IPR001936. RasGAP.
IPR000593. RasGAP_C.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00612. IQ. 4 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00015. IQ. 4 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS50096. IQ. 4 hits.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JKF1-1 [UniParc]FASTAAdd to Basket

« Hide

MSAAEEVDGL GVVRPHYGSV LDNERLTAEE MDERRRQNVA YEYLCHLEEA     50
KRWMEACLGE DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ 100
TRYKATGLHF RHTDNVIQWL NAMDEIGLPK IFYPETTDIY DRKNMPRCIY 150
CIHALSLYLF KLGLAPQIQD LYGKVDFTEE EINNMKIELE KYGIQMPAFS 200
KIGGILANEL SVDEAALHAA VIAINEAIDR RVAADTFTAL KNPNAMLVNL 250
EEGLAPTYQD VLYQAKQDKM TNAKNRTENS DRERDVYEEL LTQAEIQGNV 300
NKVNTSSALA NISLALEQGC AVTLLKALQS LALGLRGLQT QNSDWYMKQL 350
QSDLQQKRQS GQTDPLQKEE VQAGVDAANS AAQQYQRRLA AVAAINAAIQ 400
KGIAEKTVLE LMNPEAQLPQ VYPFAADLYQ KELATLQQQS PEHSLTHPEL 450
TVAVEMLSSV ALINRALESG DMTTVWKQLS SSVTGLTNIE EENCQRYLDE 500
LMKLKAQAHA ENNAFITWND IQACVDHVNL VVHEEHERIL AIGLINEALD 550
EGDAQKTLQA LQIPAAKLEG VLAEVAQHYQ DTLIRAKREK AQETQDESAV 600
LWLDEIQGGI WQSNKDTQEA QRFALGISAI NEAVDSGDVG RTLSALRSPD 650
VGLYGVIPEC GETYQSDLAE AKKKRLAAGD NNSKWVKHWV KGGYHYYHNL 700
ETQAGGWAEP PDFVQNSVQL SREEIQSSIS GVTAAYNREQ LWLANEGLIT 750
KLQACCRGYL VRQEFRSRMN FLKKQIPAIT CIQSQWRGYK QKKAYQDRLA 800
YLHSHKDEVV KIQSLARMHQ ARKRYRDRLQ YFRDHINDII KIQAFIRANK 850
ARDDYKTLIN AEDPPMIVVR KFVHLLDQSD QDFQEELDLM KMREEVITLI 900
RSNQQLENDL NLMDIKIGLL VKNKITLQDV VSHSKKLTKK NKEQLSDMMM 950
INKQKGGLKA LSKEKREKLE AYQHLFYLLQ TNPTYLAKLI FQMPQNKSTK 1000
FMDSVIFTLY NYASNQREEY LLLRLFQTAL QEEIKSKVDQ IQEIVTGNPT 1050
VIKMVVSFNR GARGQNALRQ ILAPVVKEIM DDKSLNIKTD PVDIYKSWVN 1100
QMESQTGEAS KLPYDVTPEQ ALSHEEVKTR LDNSIRNMRA VTDKFLSAIV 1150
SSVDKIPYGM RFIAKVLKDS LHEKFPDAGE DELLKIIGNL LYYRYMNPAI 1200
VAPDAFDIID LSAGGQLTTD QRRNLGSIAK MLQHAASNKM FLGDNAHLSI 1250
INEYLSQSYQ KFRRFFQVAC DVPELQDKFN VDEYSDLVTL TKPVIYISIG 1300
EIINTHTLLL DHQDAIAPEH NDPIHELLDD LGEVPTIESL IGESCGNSND 1350
PNKEALAKTE VSLTLTNKFD VPGDENAEMD ARTILLNTKR LIVDVIRFQP 1400
GETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK SKPMKEDNNL 1450
SLQEKKEKIQ TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRK 1500
AELVKLQQTY SALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE 1550
MKGKKSKKIS LKYTAARLHE KGVLLEIEDL QANQFKNVIF EIGPTEEVGD 1600
FEVKAKFMGV QMETFMLHYQ DLLQLQYEGV AVMKLFDRAK VNVNLLIFLL 1650
NKKFYGK 1657
Length:1,657
Mass (Da):188,742
Last modified:October 3, 2012 - v2
Checksum:i9136695557ADA784
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1268 – 12681V → L in AAF60344. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF240630 mRNA. Translation: AAF60344.1.
AC109257 Genomic DNA. No translation available.
AC127594 Genomic DNA. No translation available.
CH466543 Genomic DNA. Translation: EDL06977.1.
CCDSiCCDS40001.1.
RefSeqiNP_057930.2. NM_016721.2.
UniGeneiMm.207619.

Genome annotation databases

EnsembliENSMUST00000167377; ENSMUSP00000128278; ENSMUSG00000030536.
GeneIDi29875.
KEGGimmu:29875.
UCSCiuc009ibc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF240630 mRNA. Translation: AAF60344.1 .
AC109257 Genomic DNA. No translation available.
AC127594 Genomic DNA. No translation available.
CH466543 Genomic DNA. Translation: EDL06977.1 .
CCDSi CCDS40001.1.
RefSeqi NP_057930.2. NM_016721.2.
UniGenei Mm.207619.

3D structure databases

ProteinModelPortali Q9JKF1.
SMRi Q9JKF1. Positions 26-210, 962-1339, 1561-1657.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9JKF1. 8 interactions.
MINTi MINT-1563339.

PTM databases

PhosphoSitei Q9JKF1.

Proteomic databases

MaxQBi Q9JKF1.
PaxDbi Q9JKF1.
PRIDEi Q9JKF1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000167377 ; ENSMUSP00000128278 ; ENSMUSG00000030536 .
GeneIDi 29875.
KEGGi mmu:29875.
UCSCi uc009ibc.2. mouse.

Organism-specific databases

CTDi 8826.
MGIi MGI:1352757. Iqgap1.

Phylogenomic databases

eggNOGi COG5261.
GeneTreei ENSGT00600000084379.
HOVERGENi HBG052143.
InParanoidi Q9JKF1.
KOi K16848.
OMAi QDILYQA.
OrthoDBi EOG7FNC6M.
TreeFami TF313078.

Miscellaneous databases

ChiTaRSi IQGAP1. mouse.
NextBioi 307110.
PROi Q9JKF1.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9JKF1.
CleanExi MM_IQGAP1.
Genevestigatori Q9JKF1.

Family and domain databases

Gene3Di 1.10.418.10. 1 hit.
1.10.506.10. 2 hits.
4.10.270.10. 1 hit.
InterProi IPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR027417. P-loop_NTPase.
IPR001936. RasGAP.
IPR000593. RasGAP_C.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00307. CH. 1 hit.
PF00612. IQ. 4 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view ]
SMARTi SM00033. CH. 1 hit.
SM00015. IQ. 4 hits.
SM00323. RasGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50021. CH. 1 hit.
PS50096. IQ. 4 hits.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of Mus musculus Cdc42 effector protein IQGAP1."
    Bernards A.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pre-B cell.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Identification of a DOCK180-related guanine nucleotide exchange factor that is capable of mediating a positive feedback activation of Cdc42."
    Lin Q., Yang W., Baird D., Feng Q., Cerione R.A.
    J. Biol. Chem. 281:35253-35262(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC42.
  6. "The Salmonella SPI2 effector SseI mediates long-term systemic infection by modulating host cell migration."
    McLaughlin L.M., Govoni G.R., Gerke C., Gopinath S., Peng K., Laidlaw G., Chien Y.H., Jeong H.W., Li Z., Brown M.D., Sacks D.B., Monack D.
    PLoS Pathog. 5:E1000671-E1000671(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH S.TYPHIMURIUM SSEI.

Entry informationi

Entry nameiIQGA1_MOUSE
AccessioniPrimary (citable) accession number: Q9JKF1
Secondary accession number(s): G3UW45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi