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Q9JKF1 (IQGA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras GTPase-activating-like protein IQGAP1
Gene names
Name:Iqgap1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1657 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

Subunit structure

Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6 By similarity. In case of infection, interacts with S.typhimurium protein sseI. Ref.5 Ref.6

Subcellular location

Cell membrane By similarity.

Domain

Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together By similarity.

Sequence similarities

Contains 1 CH (calponin-homology) domain.

Contains 4 IQ domains.

Contains 1 Ras-GAP domain.

Contains 1 WW domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainRepeat
   LigandCalmodulin-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to calcium ion

Inferred from electronic annotation. Source: Ensembl

glomerular visceral epithelial cell development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of dephosphorylation

Inferred from genetic interaction PubMed 21709260. Source: MGI

positive regulation of protein kinase activity

Inferred from mutant phenotype PubMed 17563371. Source: BHF-UCL

regulation of cytokine production

Inferred from mutant phenotype PubMed 21709260. Source: MGI

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcell leading edge

Inferred from genetic interaction PubMed 16369480. Source: MGI

cell-cell junction

Inferred from direct assay PubMed 18604197. Source: MGI

cytoplasm

Inferred from direct assay PubMed 14516655PubMed 18604197. Source: MGI

extrinsic component of cytoplasmic side of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

lateral plasma membrane

Inferred from direct assay PubMed 18604197. Source: MGI

membrane

Inferred from direct assay PubMed 16510873. Source: MGI

microtubule

Inferred from electronic annotation. Source: Ensembl

midbody

Inferred from electronic annotation. Source: Ensembl

neuron projection

Inferred from genetic interaction PubMed 16369480. Source: MGI

nucleus

Inferred from electronic annotation. Source: Ensembl

ribonucleoprotein complex

Inferred from direct assay PubMed 21709260. Source: MGI

slit diaphragm

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionRac GTPase binding

Inferred from physical interaction PubMed 18604197. Source: MGI

Ras GTPase activator activity

Inferred from electronic annotation. Source: InterPro

Rho GTPase binding

Inferred from physical interaction PubMed 18604197. Source: MGI

phosphatidylinositol-3,4,5-trisphosphate binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 16628219PubMed 20075861. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

gagP0333217EBI-644633,EBI-935477From a different organism.
Shc1P980835EBI-644633,EBI-300201

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 16571656Ras GTPase-activating-like protein IQGAP1
PRO_0000056649

Regions

Domain44 – 159116CH
Domain685 – 71026WW
Domain745 – 77430IQ 1
Domain775 – 80430IQ 2
Domain805 – 83430IQ 3
Domain835 – 86430IQ 4
Domain1004 – 1237234Ras-GAP
Region956 – 1274319C1
Region1276 – 1657382C2

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity
Modified residue1721Phosphotyrosine Ref.4
Modified residue3301Phosphoserine By similarity

Experimental info

Sequence conflict12681V → L in AAF60344. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JKF1 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 9136695557ADA784

FASTA1,657188,742
        10         20         30         40         50         60 
MSAAEEVDGL GVVRPHYGSV LDNERLTAEE MDERRRQNVA YEYLCHLEEA KRWMEACLGE 

        70         80         90        100        110        120 
DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ TRYKATGLHF RHTDNVIQWL 

       130        140        150        160        170        180 
NAMDEIGLPK IFYPETTDIY DRKNMPRCIY CIHALSLYLF KLGLAPQIQD LYGKVDFTEE 

       190        200        210        220        230        240 
EINNMKIELE KYGIQMPAFS KIGGILANEL SVDEAALHAA VIAINEAIDR RVAADTFTAL 

       250        260        270        280        290        300 
KNPNAMLVNL EEGLAPTYQD VLYQAKQDKM TNAKNRTENS DRERDVYEEL LTQAEIQGNV 

       310        320        330        340        350        360 
NKVNTSSALA NISLALEQGC AVTLLKALQS LALGLRGLQT QNSDWYMKQL QSDLQQKRQS 

       370        380        390        400        410        420 
GQTDPLQKEE VQAGVDAANS AAQQYQRRLA AVAAINAAIQ KGIAEKTVLE LMNPEAQLPQ 

       430        440        450        460        470        480 
VYPFAADLYQ KELATLQQQS PEHSLTHPEL TVAVEMLSSV ALINRALESG DMTTVWKQLS 

       490        500        510        520        530        540 
SSVTGLTNIE EENCQRYLDE LMKLKAQAHA ENNAFITWND IQACVDHVNL VVHEEHERIL 

       550        560        570        580        590        600 
AIGLINEALD EGDAQKTLQA LQIPAAKLEG VLAEVAQHYQ DTLIRAKREK AQETQDESAV 

       610        620        630        640        650        660 
LWLDEIQGGI WQSNKDTQEA QRFALGISAI NEAVDSGDVG RTLSALRSPD VGLYGVIPEC 

       670        680        690        700        710        720 
GETYQSDLAE AKKKRLAAGD NNSKWVKHWV KGGYHYYHNL ETQAGGWAEP PDFVQNSVQL 

       730        740        750        760        770        780 
SREEIQSSIS GVTAAYNREQ LWLANEGLIT KLQACCRGYL VRQEFRSRMN FLKKQIPAIT 

       790        800        810        820        830        840 
CIQSQWRGYK QKKAYQDRLA YLHSHKDEVV KIQSLARMHQ ARKRYRDRLQ YFRDHINDII 

       850        860        870        880        890        900 
KIQAFIRANK ARDDYKTLIN AEDPPMIVVR KFVHLLDQSD QDFQEELDLM KMREEVITLI 

       910        920        930        940        950        960 
RSNQQLENDL NLMDIKIGLL VKNKITLQDV VSHSKKLTKK NKEQLSDMMM INKQKGGLKA 

       970        980        990       1000       1010       1020 
LSKEKREKLE AYQHLFYLLQ TNPTYLAKLI FQMPQNKSTK FMDSVIFTLY NYASNQREEY 

      1030       1040       1050       1060       1070       1080 
LLLRLFQTAL QEEIKSKVDQ IQEIVTGNPT VIKMVVSFNR GARGQNALRQ ILAPVVKEIM 

      1090       1100       1110       1120       1130       1140 
DDKSLNIKTD PVDIYKSWVN QMESQTGEAS KLPYDVTPEQ ALSHEEVKTR LDNSIRNMRA 

      1150       1160       1170       1180       1190       1200 
VTDKFLSAIV SSVDKIPYGM RFIAKVLKDS LHEKFPDAGE DELLKIIGNL LYYRYMNPAI 

      1210       1220       1230       1240       1250       1260 
VAPDAFDIID LSAGGQLTTD QRRNLGSIAK MLQHAASNKM FLGDNAHLSI INEYLSQSYQ 

      1270       1280       1290       1300       1310       1320 
KFRRFFQVAC DVPELQDKFN VDEYSDLVTL TKPVIYISIG EIINTHTLLL DHQDAIAPEH 

      1330       1340       1350       1360       1370       1380 
NDPIHELLDD LGEVPTIESL IGESCGNSND PNKEALAKTE VSLTLTNKFD VPGDENAEMD 

      1390       1400       1410       1420       1430       1440 
ARTILLNTKR LIVDVIRFQP GETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK 

      1450       1460       1470       1480       1490       1500 
SKPMKEDNNL SLQEKKEKIQ TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRK 

      1510       1520       1530       1540       1550       1560 
AELVKLQQTY SALNSKATFY GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE MKGKKSKKIS 

      1570       1580       1590       1600       1610       1620 
LKYTAARLHE KGVLLEIEDL QANQFKNVIF EIGPTEEVGD FEVKAKFMGV QMETFMLHYQ 

      1630       1640       1650 
DLLQLQYEGV AVMKLFDRAK VNVNLLIFLL NKKFYGK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of Mus musculus Cdc42 effector protein IQGAP1."
Bernards A.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pre-B cell.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Identification of a DOCK180-related guanine nucleotide exchange factor that is capable of mediating a positive feedback activation of Cdc42."
Lin Q., Yang W., Baird D., Feng Q., Cerione R.A.
J. Biol. Chem. 281:35253-35262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDC42.
[6]"The Salmonella SPI2 effector SseI mediates long-term systemic infection by modulating host cell migration."
McLaughlin L.M., Govoni G.R., Gerke C., Gopinath S., Peng K., Laidlaw G., Chien Y.H., Jeong H.W., Li Z., Brown M.D., Sacks D.B., Monack D.
PLoS Pathog. 5:E1000671-E1000671(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH S.TYPHIMURIUM SSEI.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF240630 mRNA. Translation: AAF60344.1.
AC109257 Genomic DNA. No translation available.
AC127594 Genomic DNA. No translation available.
CH466543 Genomic DNA. Translation: EDL06977.1.
CCDSCCDS40001.1.
RefSeqNP_057930.2. NM_016721.2.
UniGeneMm.207619.

3D structure databases

ProteinModelPortalQ9JKF1.
SMRQ9JKF1. Positions 26-210, 962-1339, 1561-1657.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9JKF1. 8 interactions.
MINTMINT-1563339.

PTM databases

PhosphoSiteQ9JKF1.

Proteomic databases

MaxQBQ9JKF1.
PaxDbQ9JKF1.
PRIDEQ9JKF1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000167377; ENSMUSP00000128278; ENSMUSG00000030536.
GeneID29875.
KEGGmmu:29875.
UCSCuc009ibc.2. mouse.

Organism-specific databases

CTD8826.
MGIMGI:1352757. Iqgap1.

Phylogenomic databases

eggNOGCOG5261.
GeneTreeENSGT00600000084379.
HOVERGENHBG052143.
InParanoidQ9JKF1.
KOK16848.
OMAQDILYQA.
OrthoDBEOG7FNC6M.
TreeFamTF313078.

Gene expression databases

ArrayExpressQ9JKF1.
CleanExMM_IQGAP1.
GenevestigatorQ9JKF1.

Family and domain databases

Gene3D1.10.418.10. 1 hit.
1.10.506.10. 2 hits.
4.10.270.10. 1 hit.
InterProIPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR027417. P-loop_NTPase.
IPR001936. RasGAP.
IPR000593. RasGAP_C.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR001202. WW_dom.
[Graphical view]
PfamPF00307. CH. 1 hit.
PF00612. IQ. 4 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]
SMARTSM00033. CH. 1 hit.
SM00015. IQ. 4 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEPS50021. CH. 1 hit.
PS50096. IQ. 4 hits.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIQGAP1. mouse.
NextBio307110.
PROQ9JKF1.
SOURCESearch...

Entry information

Entry nameIQGA1_MOUSE
AccessionPrimary (citable) accession number: Q9JKF1
Secondary accession number(s): G3UW45
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot