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Q9JKD9 (ZBT32_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 32
Alternative name(s):
Repressor of GATA
Testis zinc finger protein
Gene names
Name:Zbtb32
Synonyms:Rog, Tzfp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor By similarity. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. Ref.2

Subunit structure

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 By similarity. Interacts with GATA3. Ref.1

Subcellular location

Nucleus. Note: Located in nuclear speckles By similarity.

Tissue specificity

Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells. Ref.1

Domain

The C-terminal zinc finger domain functions as a transcriptional transactivator.

The BTB (POZ) domain possesses repressor activity.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 1 BTB (POZ) domain.

Contains 3 C2H2-type zinc fingers.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JKD9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JKD9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-364: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Zinc finger and BTB domain-containing protein 32
PRO_0000273418

Regions

Domain29 – 8759BTB
Zinc finger350 – 37223C2H2-type 1
Zinc finger378 – 40023C2H2-type 2
Zinc finger405 – 42723C2H2-type 3

Natural variations

Alternative sequence1 – 364364Missing in isoform 2.
VSP_022552

Secondary structure

.................... 465
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D72E83AB3661F403

FASTA46550,828
        10         20         30         40         50         60 
MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA SPRLGCRGRW 

        70         80         90        100        110        120 
ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL GVQALEEACQ RAQKGKDEDE 

       130        140        150        160        170        180 
LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK PEKDFRSNGR EQEMSHKHKA PGERPEMAGA 

       190        200        210        220        230        240 
TRMMSSEEVM RGIESHKGSE ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW 

       250        260        270        280        290        300 
SILLWPSRYG APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ 

       310        320        330        340        350        360 
GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY SCSVCGKRFS 

       370        380        390        400        410        420 
LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH GAAPYRCPLC RAGCPSLASM 

       430        440        450        460 
QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT RASSSPGSPT SSAAT 

« Hide

Isoform 2 [UniParc].

Checksum: F4F882ED6B5723FC
Show »

FASTA10111,021

References

« Hide 'large scale' references
[1]"ROG, repressor of GATA, regulates the expression of cytokine genes."
Miaw S.-C., Choi A., Yu E., Kishikawa H., Ho I.-C.
Immunity 12:323-333(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GATA3.
[2]"The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene."
Tang C.J., Chuang C.K., Hu H.M., Tang T.K.
J. Biol. Chem. 276:19631-19639(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION.
Strain: 129/SvJ.
Tissue: Testis.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Testis.
[4]"Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein."
Chou C.C., Lou Y.C., Tang T.K., Chen C.
Proteins 78:2202-2212(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 348-428, DNA-BINDING, ZINC-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF241232 mRNA. Translation: AAF61244.1.
AY015272 Genomic DNA. Translation: AAK13198.1.
AK015881 mRNA. Translation: BAB30015.1.
CCDSCCDS21102.1. [Q9JKD9-1]
RefSeqNP_067372.2. NM_021397.2.
UniGeneMm.116789.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KVFNMR-A348-374[»]
2KVGNMR-A402-428[»]
2KVHNMR-A375-401[»]
ProteinModelPortalQ9JKD9.
SMRQ9JKD9. Positions 5-113, 336-428.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4084416.

PTM databases

PhosphoSiteQ9JKD9.

Proteomic databases

PRIDEQ9JKD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310. [Q9JKD9-2]
GeneID58206.
KEGGmmu:58206.
UCSCuc009gfi.1. mouse. [Q9JKD9-2]

Organism-specific databases

CTD27033.
MGIMGI:1891838. Zbtb32.

Phylogenomic databases

eggNOGNOG296621.
GeneTreeENSGT00530000063338.
HOGENOMHOG000060112.
HOVERGENHBG079944.
InParanoidQ9JKD9.
KOK10506.
PhylomeDBQ9JKD9.

Gene expression databases

ArrayExpressQ9JKD9.
BgeeQ9JKD9.
CleanExMM_ZBTB32.
GenevestigatorQ9JKD9.

Family and domain databases

Gene3D3.30.160.60. 2 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9JKD9.
NextBio314193.
PROQ9JKD9.
SOURCESearch...

Entry information

Entry nameZBT32_MOUSE
AccessionPrimary (citable) accession number: Q9JKD9
Secondary accession number(s): Q9D523
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot