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Protein

Zinc finger and BTB domain-containing protein 32

Gene

Zbtb32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor (By similarity). Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri405 – 42723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. transcription corepressor activity Source: MGI
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. hemopoiesis Source: MGI
  2. negative regulation of nucleic acid-templated transcription Source: GOC
  3. regulation of cytokine production Source: MGI
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. T cell proliferation Source: MGI
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_329081. Regulation of the Fanconi anemia pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 32
Alternative name(s):
Repressor of GATA
Testis zinc finger protein
Gene namesi
Name:Zbtb32
Synonyms:Rog, Tzfp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1891838. Zbtb32.

Subcellular locationi

Nucleus
Note: Located in nuclear speckles.By similarity

GO - Cellular componenti

  1. nuclear chromosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Zinc finger and BTB domain-containing protein 32PRO_0000273418Add
BLAST

Proteomic databases

PRIDEiQ9JKD9.

PTM databases

PhosphoSiteiQ9JKD9.

Expressioni

Tissue specificityi

Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells.1 Publication

Gene expression databases

BgeeiQ9JKD9.
CleanExiMM_ZBTB32.
ExpressionAtlasiQ9JKD9. baseline and differential.
GenevestigatoriQ9JKD9.

Interactioni

Subunit structurei

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 (By similarity). Interacts with GATA3.By similarity1 Publication

Protein-protein interaction databases

MINTiMINT-4084416.

Structurei

Secondary structure

1
465
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi349 – 3513Combined sources
Beta strandi353 – 3553Combined sources
Beta strandi358 – 3603Combined sources
Helixi362 – 3698Combined sources
Beta strandi381 – 3844Combined sources
Beta strandi386 – 3894Combined sources
Helixi390 – 39910Combined sources
Beta strandi405 – 4073Combined sources
Turni408 – 4114Combined sources
Beta strandi412 – 4154Combined sources
Helixi417 – 4248Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KVFNMR-A348-374[»]
2KVGNMR-A402-428[»]
2KVHNMR-A375-401[»]
ProteinModelPortaliQ9JKD9.
SMRiQ9JKD9. Positions 5-113, 336-428.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JKD9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 8759BTBPROSITE-ProRule annotationAdd
BLAST

Domaini

The C-terminal zinc finger domain functions as a transcriptional transactivator.
The BTB (POZ) domain possesses repressor activity.

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri405 – 42723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG296621.
GeneTreeiENSGT00530000063338.
HOGENOMiHOG000060112.
HOVERGENiHBG079944.
InParanoidiQ9JKD9.
KOiK10506.
PhylomeDBiQ9JKD9.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JKD9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA
60 70 80 90 100
SPRLGCRGRW ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL
110 120 130 140 150
GVQALEEACQ RAQKGKDEDE LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK
160 170 180 190 200
PEKDFRSNGR EQEMSHKHKA PGERPEMAGA TRMMSSEEVM RGIESHKGSE
210 220 230 240 250
ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW SILLWPSRYG
260 270 280 290 300
APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ
310 320 330 340 350
GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY
360 370 380 390 400
SCSVCGKRFS LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH
410 420 430 440 450
GAAPYRCPLC RAGCPSLASM QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT
460
RASSSPGSPT SSAAT
Length:465
Mass (Da):50,828
Last modified:October 1, 2000 - v1
Checksum:iD72E83AB3661F403
GO
Isoform 2 (identifier: Q9JKD9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-364: Missing.

Note: No experimental confirmation available.

Show »
Length:101
Mass (Da):11,021
Checksum:iF4F882ED6B5723FC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 364364Missing in isoform 2. 1 PublicationVSP_022552Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241232 mRNA. Translation: AAF61244.1.
AY015272 Genomic DNA. Translation: AAK13198.1.
AK015881 mRNA. Translation: BAB30015.1.
CCDSiCCDS21102.1. [Q9JKD9-1]
RefSeqiNP_067372.2. NM_021397.2.
UniGeneiMm.116789.

Genome annotation databases

EnsembliENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310. [Q9JKD9-2]
GeneIDi58206.
KEGGimmu:58206.
UCSCiuc009gfi.1. mouse. [Q9JKD9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241232 mRNA. Translation: AAF61244.1.
AY015272 Genomic DNA. Translation: AAK13198.1.
AK015881 mRNA. Translation: BAB30015.1.
CCDSiCCDS21102.1. [Q9JKD9-1]
RefSeqiNP_067372.2. NM_021397.2.
UniGeneiMm.116789.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KVFNMR-A348-374[»]
2KVGNMR-A402-428[»]
2KVHNMR-A375-401[»]
ProteinModelPortaliQ9JKD9.
SMRiQ9JKD9. Positions 5-113, 336-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4084416.

PTM databases

PhosphoSiteiQ9JKD9.

Proteomic databases

PRIDEiQ9JKD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310. [Q9JKD9-2]
GeneIDi58206.
KEGGimmu:58206.
UCSCiuc009gfi.1. mouse. [Q9JKD9-2]

Organism-specific databases

CTDi27033.
MGIiMGI:1891838. Zbtb32.

Phylogenomic databases

eggNOGiNOG296621.
GeneTreeiENSGT00530000063338.
HOGENOMiHOG000060112.
HOVERGENiHBG079944.
InParanoidiQ9JKD9.
KOiK10506.
PhylomeDBiQ9JKD9.

Enzyme and pathway databases

ReactomeiREACT_329081. Regulation of the Fanconi anemia pathway.

Miscellaneous databases

EvolutionaryTraceiQ9JKD9.
NextBioi314193.
PROiQ9JKD9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JKD9.
CleanExiMM_ZBTB32.
ExpressionAtlasiQ9JKD9. baseline and differential.
GenevestigatoriQ9JKD9.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ROG, repressor of GATA, regulates the expression of cytokine genes."
    Miaw S.-C., Choi A., Yu E., Kishikawa H., Ho I.-C.
    Immunity 12:323-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GATA3.
  2. "The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene."
    Tang C.J., Chuang C.K., Hu H.M., Tang T.K.
    J. Biol. Chem. 276:19631-19639(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION.
    Strain: 129/SvJ.
    Tissue: Testis.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  4. "Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein."
    Chou C.C., Lou Y.C., Tang T.K., Chen C.
    Proteins 78:2202-2212(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 348-428, DNA-BINDING, ZINC-BINDING.

Entry informationi

Entry nameiZBT32_MOUSE
AccessioniPrimary (citable) accession number: Q9JKD9
Secondary accession number(s): Q9D523
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.