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Protein

Zinc finger and BTB domain-containing protein 32

Gene

Zbtb32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor (By similarity). Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri350 – 372C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri378 – 400C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri405 – 427C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • transcription corepressor activity Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • hemopoiesis Source: MGI
  • regulation of cytokine production Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • T cell proliferation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-6783310. Fanconi Anemia Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 32
Alternative name(s):
Repressor of GATA
Testis zinc finger protein
Gene namesi
Name:Zbtb32
Synonyms:Rog, Tzfp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1891838. Zbtb32.

Subcellular locationi

  • Nucleus

  • Note: Located in nuclear speckles.By similarity

GO - Cellular componenti

  • nuclear chromosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002734181 – 465Zinc finger and BTB domain-containing protein 32Add BLAST465

Proteomic databases

EPDiQ9JKD9.
PaxDbiQ9JKD9.
PRIDEiQ9JKD9.

PTM databases

iPTMnetiQ9JKD9.
PhosphoSitePlusiQ9JKD9.

Expressioni

Tissue specificityi

Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000006310.
CleanExiMM_ZBTB32.
ExpressionAtlasiQ9JKD9. baseline and differential.
GenevisibleiQ9JKD9. MM.

Interactioni

Subunit structurei

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 (By similarity). Interacts with GATA3.By similarity1 Publication

Protein-protein interaction databases

BioGridi208389. 1 interactor.
MINTiMINT-4084416.
STRINGi10090.ENSMUSP00000103786.

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi349 – 351Combined sources3
Beta strandi353 – 355Combined sources3
Beta strandi358 – 360Combined sources3
Helixi362 – 369Combined sources8
Beta strandi381 – 384Combined sources4
Beta strandi386 – 389Combined sources4
Helixi390 – 399Combined sources10
Beta strandi405 – 407Combined sources3
Turni408 – 411Combined sources4
Beta strandi412 – 415Combined sources4
Helixi417 – 424Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KVFNMR-A348-374[»]
2KVGNMR-A402-428[»]
2KVHNMR-A375-401[»]
ProteinModelPortaliQ9JKD9.
SMRiQ9JKD9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JKD9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 87BTBPROSITE-ProRule annotationAdd BLAST59

Domaini

The C-terminal zinc finger domain functions as a transcriptional transactivator.
The BTB (POZ) domain possesses repressor activity.

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri350 – 372C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri378 – 400C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri405 – 427C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063338.
HOGENOMiHOG000060112.
HOVERGENiHBG079944.
InParanoidiQ9JKD9.
KOiK10506.
PhylomeDBiQ9JKD9.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JKD9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA
60 70 80 90 100
SPRLGCRGRW ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL
110 120 130 140 150
GVQALEEACQ RAQKGKDEDE LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK
160 170 180 190 200
PEKDFRSNGR EQEMSHKHKA PGERPEMAGA TRMMSSEEVM RGIESHKGSE
210 220 230 240 250
ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW SILLWPSRYG
260 270 280 290 300
APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ
310 320 330 340 350
GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY
360 370 380 390 400
SCSVCGKRFS LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH
410 420 430 440 450
GAAPYRCPLC RAGCPSLASM QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT
460
RASSSPGSPT SSAAT
Length:465
Mass (Da):50,828
Last modified:October 1, 2000 - v1
Checksum:iD72E83AB3661F403
GO
Isoform 2 (identifier: Q9JKD9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-364: Missing.

Note: No experimental confirmation available.
Show »
Length:101
Mass (Da):11,021
Checksum:iF4F882ED6B5723FC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0225521 – 364Missing in isoform 2. 1 PublicationAdd BLAST364

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241232 mRNA. Translation: AAF61244.1.
AY015272 Genomic DNA. Translation: AAK13198.1.
AK015881 mRNA. Translation: BAB30015.1.
CCDSiCCDS21102.1. [Q9JKD9-1]
RefSeqiNP_067372.2. NM_021397.2.
UniGeneiMm.116789.

Genome annotation databases

EnsembliENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310. [Q9JKD9-2]
GeneIDi58206.
KEGGimmu:58206.
UCSCiuc009gfi.1. mouse. [Q9JKD9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241232 mRNA. Translation: AAF61244.1.
AY015272 Genomic DNA. Translation: AAK13198.1.
AK015881 mRNA. Translation: BAB30015.1.
CCDSiCCDS21102.1. [Q9JKD9-1]
RefSeqiNP_067372.2. NM_021397.2.
UniGeneiMm.116789.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KVFNMR-A348-374[»]
2KVGNMR-A402-428[»]
2KVHNMR-A375-401[»]
ProteinModelPortaliQ9JKD9.
SMRiQ9JKD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208389. 1 interactor.
MINTiMINT-4084416.
STRINGi10090.ENSMUSP00000103786.

PTM databases

iPTMnetiQ9JKD9.
PhosphoSitePlusiQ9JKD9.

Proteomic databases

EPDiQ9JKD9.
PaxDbiQ9JKD9.
PRIDEiQ9JKD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310. [Q9JKD9-2]
GeneIDi58206.
KEGGimmu:58206.
UCSCiuc009gfi.1. mouse. [Q9JKD9-2]

Organism-specific databases

CTDi27033.
MGIiMGI:1891838. Zbtb32.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063338.
HOGENOMiHOG000060112.
HOVERGENiHBG079944.
InParanoidiQ9JKD9.
KOiK10506.
PhylomeDBiQ9JKD9.

Enzyme and pathway databases

ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-6783310. Fanconi Anemia Pathway.

Miscellaneous databases

EvolutionaryTraceiQ9JKD9.
PROiQ9JKD9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006310.
CleanExiMM_ZBTB32.
ExpressionAtlasiQ9JKD9. baseline and differential.
GenevisibleiQ9JKD9. MM.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZBT32_MOUSE
AccessioniPrimary (citable) accession number: Q9JKD9
Secondary accession number(s): Q9D523
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.