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Q9JKD9

- ZBT32_MOUSE

UniProt

Q9JKD9 - ZBT32_MOUSE

Protein

Zinc finger and BTB domain-containing protein 32

Gene

Zbtb32

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor By similarity. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri405 – 42723C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. transcription corepressor activity Source: MGI
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. hemopoiesis Source: MGI
    2. regulation of cytokine production Source: MGI
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. T cell proliferation Source: MGI
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger and BTB domain-containing protein 32
    Alternative name(s):
    Repressor of GATA
    Testis zinc finger protein
    Gene namesi
    Name:Zbtb32
    Synonyms:Rog, Tzfp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1891838. Zbtb32.

    Subcellular locationi

    Nucleus
    Note: Located in nuclear speckles.By similarity

    GO - Cellular componenti

    1. nuclear chromosome Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 465465Zinc finger and BTB domain-containing protein 32PRO_0000273418Add
    BLAST

    Proteomic databases

    PRIDEiQ9JKD9.

    PTM databases

    PhosphoSiteiQ9JKD9.

    Expressioni

    Tissue specificityi

    Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells.1 Publication

    Gene expression databases

    ArrayExpressiQ9JKD9.
    BgeeiQ9JKD9.
    CleanExiMM_ZBTB32.
    GenevestigatoriQ9JKD9.

    Interactioni

    Subunit structurei

    Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 By similarity. Interacts with GATA3.By similarity1 Publication

    Protein-protein interaction databases

    MINTiMINT-4084416.

    Structurei

    Secondary structure

    1
    465
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi349 – 3513
    Beta strandi353 – 3553
    Beta strandi358 – 3603
    Helixi362 – 3698
    Beta strandi381 – 3844
    Beta strandi386 – 3894
    Helixi390 – 39910
    Beta strandi405 – 4073
    Turni408 – 4114
    Beta strandi412 – 4154
    Helixi417 – 4248

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KVFNMR-A348-374[»]
    2KVGNMR-A402-428[»]
    2KVHNMR-A375-401[»]
    ProteinModelPortaliQ9JKD9.
    SMRiQ9JKD9. Positions 5-113, 336-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9JKD9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 8759BTBPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri350 – 37223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri378 – 40023C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri405 – 42723C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG296621.
    GeneTreeiENSGT00530000063338.
    HOGENOMiHOG000060112.
    HOVERGENiHBG079944.
    InParanoidiQ9JKD9.
    KOiK10506.
    PhylomeDBiQ9JKD9.

    Family and domain databases

    Gene3Di3.30.160.60. 2 hits.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JKD9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA    50
    SPRLGCRGRW ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL 100
    GVQALEEACQ RAQKGKDEDE LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK 150
    PEKDFRSNGR EQEMSHKHKA PGERPEMAGA TRMMSSEEVM RGIESHKGSE 200
    ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW SILLWPSRYG 250
    APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ 300
    GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY 350
    SCSVCGKRFS LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH 400
    GAAPYRCPLC RAGCPSLASM QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT 450
    RASSSPGSPT SSAAT 465
    Length:465
    Mass (Da):50,828
    Last modified:October 1, 2000 - v1
    Checksum:iD72E83AB3661F403
    GO
    Isoform 2 (identifier: Q9JKD9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-364: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:101
    Mass (Da):11,021
    Checksum:iF4F882ED6B5723FC
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 364364Missing in isoform 2. 1 PublicationVSP_022552Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241232 mRNA. Translation: AAF61244.1.
    AY015272 Genomic DNA. Translation: AAK13198.1.
    AK015881 mRNA. Translation: BAB30015.1.
    CCDSiCCDS21102.1. [Q9JKD9-1]
    RefSeqiNP_067372.2. NM_021397.2.
    UniGeneiMm.116789.

    Genome annotation databases

    EnsembliENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310. [Q9JKD9-2]
    GeneIDi58206.
    KEGGimmu:58206.
    UCSCiuc009gfi.1. mouse. [Q9JKD9-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241232 mRNA. Translation: AAF61244.1 .
    AY015272 Genomic DNA. Translation: AAK13198.1 .
    AK015881 mRNA. Translation: BAB30015.1 .
    CCDSi CCDS21102.1. [Q9JKD9-1 ]
    RefSeqi NP_067372.2. NM_021397.2.
    UniGenei Mm.116789.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KVF NMR - A 348-374 [» ]
    2KVG NMR - A 402-428 [» ]
    2KVH NMR - A 375-401 [» ]
    ProteinModelPortali Q9JKD9.
    SMRi Q9JKD9. Positions 5-113, 336-428.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4084416.

    PTM databases

    PhosphoSitei Q9JKD9.

    Proteomic databases

    PRIDEi Q9JKD9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108150 ; ENSMUSP00000103785 ; ENSMUSG00000006310 . [Q9JKD9-2 ]
    GeneIDi 58206.
    KEGGi mmu:58206.
    UCSCi uc009gfi.1. mouse. [Q9JKD9-2 ]

    Organism-specific databases

    CTDi 27033.
    MGIi MGI:1891838. Zbtb32.

    Phylogenomic databases

    eggNOGi NOG296621.
    GeneTreei ENSGT00530000063338.
    HOGENOMi HOG000060112.
    HOVERGENi HBG079944.
    InParanoidi Q9JKD9.
    KOi K10506.
    PhylomeDBi Q9JKD9.

    Miscellaneous databases

    EvolutionaryTracei Q9JKD9.
    NextBioi 314193.
    PROi Q9JKD9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JKD9.
    Bgeei Q9JKD9.
    CleanExi MM_ZBTB32.
    Genevestigatori Q9JKD9.

    Family and domain databases

    Gene3Di 3.30.160.60. 2 hits.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ROG, repressor of GATA, regulates the expression of cytokine genes."
      Miaw S.-C., Choi A., Yu E., Kishikawa H., Ho I.-C.
      Immunity 12:323-333(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GATA3.
    2. "The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene."
      Tang C.J., Chuang C.K., Hu H.M., Tang T.K.
      J. Biol. Chem. 276:19631-19639(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION.
      Strain: 129/SvJ.
      Tissue: Testis.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    4. "Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein."
      Chou C.C., Lou Y.C., Tang T.K., Chen C.
      Proteins 78:2202-2212(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 348-428, DNA-BINDING, ZINC-BINDING.

    Entry informationi

    Entry nameiZBT32_MOUSE
    AccessioniPrimary (citable) accession number: Q9JKD9
    Secondary accession number(s): Q9D523
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3