Q9JKD9 (ZBT32_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger and BTB domain-containing protein 32

Alternative name(s):
Repressor of GATA
Testis zinc finger protein

Gene names

Name:	Zbtb32
Synonyms:	Rog, Tzfp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor By similarity. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. Ref.2
Subunit structure	Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 By similarity. Interacts with GATA3. Ref.1
Subcellular location	Nucleus. Note: Located in nuclear speckles By similarity.
Tissue specificity	Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells. Ref.1
Domain	The C-terminal zinc finger domain functions as a transcriptional transactivator. The BTB (POZ) domain possesses repressor activity.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell proliferation Inferred from mutant phenotype PubMed 15542853. Source: MGI hemopoiesis Inferred from mutant phenotype PubMed 15542853. Source: MGI regulation of cytokine production Inferred from mutant phenotype PubMed 15542853. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nuclear chromosome Inferred from direct assay PubMed 11555636. Source: MGI
Molecular_function	DNA binding Inferred from direct assay Ref.4. Source: UniProtKB transcription corepressor activity Inferred from direct assay Ref.1. Source: MGI zinc ion binding Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JKD9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JKD9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-364: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 465

465

Zinc finger and BTB domain-containing protein 32

PRO_0000273418

Regions

Domain

29 – 87

59

BTB

Zinc finger

350 – 372

23

C2H2-type 1

Zinc finger

378 – 400

23

C2H2-type 2

Zinc finger

405 – 427

23

C2H2-type 3

Natural variations

Alternative sequence

1 – 364

364

Missing in isoform 2.

VSP_022552

Secondary structure

1

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465

Helix Strand Turn

Details...

Beta strand

349 – 351

3

Beta strand

353 – 355

3

Beta strand

358 – 360

3

Helix

362 – 369

8

Beta strand

381 – 384

4

Beta strand

386 – 389

4

Helix

390 – 399

10

Beta strand

405 – 407

3

Turn

408 – 411

4

Beta strand

412 – 415

4

Helix

417 – 424

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D72E83AB3661F403	FASTA	465	50,828
	10 20 30 40 50 60 MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA SPRLGCRGRW 70 80 90 100 110 120 ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL GVQALEEACQ RAQKGKDEDE 130 140 150 160 170 180 LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK PEKDFRSNGR EQEMSHKHKA PGERPEMAGA 190 200 210 220 230 240 TRMMSSEEVM RGIESHKGSE ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW 250 260 270 280 290 300 SILLWPSRYG APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ 310 320 330 340 350 360 GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY SCSVCGKRFS 370 380 390 400 410 420 LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH GAAPYRCPLC RAGCPSLASM 430 440 450 460 QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT RASSSPGSPT SSAAT
	Isoform 2 [UniParc]. Checksum: F4F882ED6B5723FC	FASTA	101	11,021
	10 20 30 40 50 60 METHYRVHTG EKPFSCSLCP QRSRDFSAMT KHLRTHGAAP YRCPLCRAGC PSLASMQAHM 70 80 90 100 RGHSPSRLPP GWTIRSTFLY SSSRPTRASS SPGSPTSSAA T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ROG, repressor of GATA, regulates the expression of cytokine genes." Miaw S.-C., Choi A., Yu E., Kishikawa H., Ho I.-C. Immunity 12:323-333(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GATA3.
[2]	"The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene." Tang C.J., Chuang C.K., Hu H.M., Tang T.K. J. Biol. Chem. 276:19631-19639(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION. Strain: 129/SvJ. Tissue: Testis.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[4]	"Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein." Chou C.C., Lou Y.C., Tang T.K., Chen C. Proteins 78:2202-2212(2010) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 348-428, DNA-BINDING, ZINC-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF241232 mRNA. Translation: AAF61244.1.
AY015272 Genomic DNA. Translation: AAK13198.1.
AK015881 mRNA. Translation: BAB30015.1.

IPI

IPI00122873.
IPI00828502.

RefSeq

NP_067372.2. NM_021397.2.

UniGene

Mm.116789.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KVF	NMR	-	A	348-374	[»]
2KVG	NMR	-	A	402-428	[»]
2KVH	NMR	-	A	375-401	[»]

ProteinModelPortal

Q9JKD9.

SMR

Q9JKD9. Positions 5-113, 348-428.

ModBase

Search...

PTM databases

PhosphoSite

Q9JKD9.

Proteomic databases

PRIDE

Q9JKD9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310.

GeneID

58206.

KEGG

mmu:58206.

UCSC

uc009gfi.1. mouse.

Organism-specific databases

CTD

27033.

MGI

MGI:1891838. Zbtb32.

Phylogenomic databases

eggNOG

NOG296621.

GeneTree

ENSGT00530000063338.

HOGENOM

HOG000060112.

HOVERGEN

HBG079944.

InParanoid

Q9JKD9.

KO

K10506.

OrthoDB

EOG402WS2.

Gene expression databases

ArrayExpress

Q9JKD9.

Bgee

Q9JKD9.

CleanEx

MM_ZBTB32.

Genevestigator

Q9JKD9.

Family and domain databases

Gene3D

3.30.160.60. 2 hits.
3.30.710.10. 1 hit.

InterPro

IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Pfam

PF00651. BTB. 1 hit.
[Graphical view]

SMART

SM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]

SUPFAM

SSF54695. BTB/POZ_fold. 1 hit.

PROSITE

PS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9JKD9.

NextBio

314193.

SOURCE

Search...

Entry information

Entry name

ZBT32_MOUSE

Accession

Primary (citable) accession number: Q9JKD9
Secondary accession number(s): Q9D523

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program