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Protein

Zinc finger and BTB domain-containing protein 32

Gene

Zbtb32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor (By similarity). Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri350 – 372C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri378 – 400C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri405 – 427C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • transcription corepressor activity Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • hemopoiesis Source: MGI
  • regulation of cytokine production Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • T cell proliferation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 32
Alternative name(s):
Repressor of GATA
Testis zinc finger protein
Gene namesi
Name:Zbtb32
Synonyms:Rog, Tzfp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1891838 Zbtb32

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002734181 – 465Zinc finger and BTB domain-containing protein 32Add BLAST465

Proteomic databases

EPDiQ9JKD9
PaxDbiQ9JKD9
PRIDEiQ9JKD9

PTM databases

iPTMnetiQ9JKD9
PhosphoSitePlusiQ9JKD9

Expressioni

Tissue specificityi

Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000006310
CleanExiMM_ZBTB32
ExpressionAtlasiQ9JKD9 baseline and differential
GenevisibleiQ9JKD9 MM

Interactioni

Subunit structurei

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 (By similarity). Interacts with GATA3.By similarity1 Publication

Protein-protein interaction databases

BioGridi208389, 1 interactor
STRINGi10090.ENSMUSP00000103786

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi349 – 351Combined sources3
Beta strandi353 – 355Combined sources3
Beta strandi358 – 360Combined sources3
Helixi362 – 369Combined sources8
Beta strandi381 – 384Combined sources4
Beta strandi386 – 389Combined sources4
Helixi390 – 399Combined sources10
Beta strandi405 – 407Combined sources3
Turni408 – 411Combined sources4
Beta strandi412 – 415Combined sources4
Helixi417 – 424Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KVFNMR-A348-374[»]
2KVGNMR-A402-428[»]
2KVHNMR-A375-401[»]
ProteinModelPortaliQ9JKD9
SMRiQ9JKD9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JKD9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 87BTBPROSITE-ProRule annotationAdd BLAST59

Domaini

The C-terminal zinc finger domain functions as a transcriptional transactivator.
The BTB (POZ) domain possesses repressor activity.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri350 – 372C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri378 – 400C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri405 – 427C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00530000063338
HOGENOMiHOG000060112
HOVERGENiHBG079944
InParanoidiQ9JKD9
KOiK10506
PhylomeDBiQ9JKD9

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF54695 SSF54695, 1 hit
SSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 2 hits
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JKD9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA
60 70 80 90 100
SPRLGCRGRW ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL
110 120 130 140 150
GVQALEEACQ RAQKGKDEDE LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK
160 170 180 190 200
PEKDFRSNGR EQEMSHKHKA PGERPEMAGA TRMMSSEEVM RGIESHKGSE
210 220 230 240 250
ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW SILLWPSRYG
260 270 280 290 300
APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ
310 320 330 340 350
GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY
360 370 380 390 400
SCSVCGKRFS LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH
410 420 430 440 450
GAAPYRCPLC RAGCPSLASM QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT
460
RASSSPGSPT SSAAT
Length:465
Mass (Da):50,828
Last modified:October 1, 2000 - v1
Checksum:iD72E83AB3661F403
GO
Isoform 2 (identifier: Q9JKD9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-364: Missing.

Note: No experimental confirmation available.
Show »
Length:101
Mass (Da):11,021
Checksum:iF4F882ED6B5723FC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0225521 – 364Missing in isoform 2. 1 PublicationAdd BLAST364

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241232 mRNA Translation: AAF61244.1
AY015272 Genomic DNA Translation: AAK13198.1
AK015881 mRNA Translation: BAB30015.1
CCDSiCCDS21102.1 [Q9JKD9-1]
RefSeqiNP_067372.2, NM_021397.2
UniGeneiMm.116789
Mm.452815

Genome annotation databases

EnsembliENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310 [Q9JKD9-2]
GeneIDi58206
KEGGimmu:58206
UCSCiuc009gfi.1 mouse [Q9JKD9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZBT32_MOUSE
AccessioniPrimary (citable) accession number: Q9JKD9
Secondary accession number(s): Q9D523
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health