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Protein

Synergin gamma

Gene

Synrg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synergin gamma
Alternative name(s):
AP1 subunit gamma-binding protein 1
Gamma-synergin
Gene namesi
Name:Synrg
Synonyms:Ap1gbp1, Syng
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620684. Synrg.

Subcellular locationi

GO - Cellular componenti

  • clathrin coat of trans-Golgi network vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13291329Synergin gammaPRO_0000072389Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei571 – 5711PhosphoserineBy similarity
Modified residuei609 – 6091N6-acetyllysineBy similarity
Modified residuei676 – 6761PhosphoserineBy similarity
Modified residuei815 – 8151PhosphoserineBy similarity
Modified residuei836 – 8361N6-acetyllysineBy similarity
Modified residuei844 – 8441PhosphoserineBy similarity
Modified residuei904 – 9041PhosphoserineCombined sources
Modified residuei944 – 9441PhosphoserineBy similarity
Modified residuei947 – 9471PhosphoserineBy similarity
Modified residuei997 – 9971PhosphoserineCombined sources
Modified residuei1088 – 10881PhosphoserineCombined sources
Modified residuei1090 – 10901PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JKC9.
PRIDEiQ9JKC9.

PTM databases

iPTMnetiQ9JKC9.

Expressioni

Tissue specificityi

Detected in brain and liver (at protein level). Ubiquitously expressed.1 Publication

Interactioni

Subunit structurei

Interacts with GGA1, GGA2 and GGA3. Interacts with the AP1G1 and AP1G2 subunits of the adapter protein complexes AP-1 (By similarity). Interacts with SCAMP1 via its EH-domain.By similarity1 Publication

Protein-protein interaction databases

BioGridi249979. 2 interactions.
MINTiMINT-130277.
STRINGi10116.ENSRNOP00000052759.

Structurei

3D structure databases

ProteinModelPortaliQ9JKC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini393 – 504112EHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni614 – 878265Interaction with A1P1G1 and A1P1G2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili112 – 15241Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi555 – 5595DFXDF motif 1
Motifi785 – 7895DFXDF motif 2
Motifi867 – 8715DFXDF motif 3

Domaini

The DFXDF motifs mediate the interaction with gamma-appendage subunits AP1G1 and AP1G2.By similarity

Sequence similaritiesi

Contains 1 EH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0998. Eukaryota.
ENOG410XTDR. LUCA.
HOGENOMiHOG000293152.
HOVERGENiHBG055053.
InParanoidiQ9JKC9.
PhylomeDBiQ9JKC9.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR000261. EH_dom.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50031. EH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JKC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRPGAGAS GAAGAGTGPG GAGSFMFPVA GGMRPPQGLI PMQQQGFPMV
60 70 80 90 100
SVMQPNMQGM MGMNYSSQMS QGPIAMQAGI PMGPMPAAGV PFLGQPPFLG
110 120 130 140 150
MRPAAPQYTP DMQKQFAEEQ QKRFEQQQKL LEEERKRRQF EEQKQKLRLL
160 170 180 190 200
SSVKPKTGEK NRDDALEAIK GNLDGFSRDA KMHPTPASHP KKPDCPTSSH
210 220 230 240 250
STKTVSPSSA FLGEDEFSGF MQGPVELPTC GPSSTAQPFQ SFLPSTPLGQ
260 270 280 290 300
LHTQKAGAQP LPPGQAPVSF AVHGVHGQIP CLSAASASHS MQKAGPSLEE
310 320 330 340 350
KLLVSCDISA SGQEHIKLSS PEAGHRAVVP GSSKNSPGLM AHNGGAVDGC
360 370 380 390 400
VSGPTTAVAE KTSDQNLSKE ESGVGVFPSQ DPVQPRMPPW IYNESLVPDA
410 420 430 440 450
YKKILETTMT PTGIDTAKLY PILMSSGLPR ETLGQIWALA NRTTPGKLTK
460 470 480 490 500
EELYTVLAMV AVTQRGVPAM SPDTLNQFPA APIPTLSGFP MTLPTPVSQP
510 520 530 540 550
TAMTSGPAGS IPLSLGQPIM GINLVGPVGG AAAPTSSGFM PAYPSNQVGK
560 570 580 590 600
TEEDDFQDFQ DASKSGSIDD SFTDFQEVPA SSKTSNSQHG NSAPSLLIPL
610 620 630 640 650
PGTKASTDKY AVFKGISAEK PSENPASFGE SGDKYSAFRE LEPTADSKPL
660 670 680 690 700
GESFAEFRST GTDDGFTDFK TADSVSPLEP PTKDSFPSAF ASGAAQQTQT
710 720 730 740 750
QVKTPLNLAD LDMFSSVDCS GEKPVPFSAA FSTSKSVSSR PQPAGSAAAP
760 770 780 790 800
ASLASTKASS LADDFGEFNL FGEYSNPASV GEQDDFADFM AFGNSSIPSE
810 820 830 840 850
PKADDKYEAL REEGSPGALS TSTVEGAHNP PVSSSKYDVF KQLSLEGAGL
860 870 880 890 900
AIEEFKENTP STKSDGDFAD FHSSKFSSTS SDKSLGEKAV AFRHAKEDSA
910 920 930 940 950
SVKSLDLPSI GGSSVGKEDS EDALSVQFDM KLADVGGDLK HVMSDSSLDL
960 970 980 990 1000
PTVSGQHPPA AGSALASEDA LPETPFPAFA SFKDMMPQTT EQKEYESGDF
1010 1020 1030 1040 1050
QDFTRQDMPM VDRSQENTCP SPASSVASHE TPKEGADDFG EFQSEKPKIS
1060 1070 1080 1090 1100
KFDFLVANSQ SKMKSSEEMI KSELATFDLS VQGSHKRSLS LGDKEISRSS
1110 1120 1130 1140 1150
PSPALEQPFR DRSNTLSERA ALPVIRDKYK DLTGEVEENE RYAYEWQRCL
1160 1170 1180 1190 1200
GSALDVIKKA NDTLNGISSS AVCTEVIQSA QGMEYLLGVV EVYRVTKRVE
1210 1220 1230 1240 1250
LGIKATAVCS EKLQQLLKDI DKVWNNLIGF MSLTTLTPDE NSLDFSSCML
1260 1270 1280 1290 1300
RPGIKNAQEL ACGVCLLNVD SRSRKEETPA EEQPKKAFNS ETDSFKLAYG
1310 1320
GHQYHASCAN FWINCVEPKP PGLLLPDLL
Length:1,329
Mass (Da):141,359
Last modified:February 6, 2007 - v3
Checksum:iD0B06BBF0CDAE083
GO
Isoform 2 (identifier: Q9JKC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-372: Missing.

Show »
Length:1,150
Mass (Da):123,249
Checksum:iE502D0602442BCB9
GO
Isoform 3 (identifier: Q9JKC9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-372: Missing.
     1275-1286: Missing.

Show »
Length:1,138
Mass (Da):121,853
Checksum:i91E5D478787F7C0D
GO
Isoform 4 (identifier: Q9JKC9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-294: Missing.
     1275-1286: Missing.

Show »
Length:1,216
Mass (Da):129,679
Checksum:i8B4F548B924EA028
GO

Sequence cautioni

The sequence AAF61257.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei194 – 372179Missing in isoform 2 and isoform 3. 1 PublicationVSP_023017Add
BLAST
Alternative sequencei194 – 294101Missing in isoform 4. 1 PublicationVSP_023018Add
BLAST
Alternative sequencei1275 – 128612Missing in isoform 3 and isoform 4. 1 PublicationVSP_023019Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF121979 mRNA. Translation: ABL63418.1.
EF121977 mRNA. Translation: ABL63416.1.
EF121978 mRNA. Translation: ABL63417.1.
EF121980 mRNA. Translation: ABL63419.1.
EF121981 mRNA. Translation: ABL63420.1.
EF121982 mRNA. Translation: ABL63421.1.
AF242544 mRNA. Translation: AAF61257.1. Different initiation.
AF169549 mRNA. Translation: AAD49733.1.
RefSeqiNP_445871.1. NM_053419.1. [Q9JKC9-1]
UniGeneiRn.122513.

Genome annotation databases

GeneIDi84479.
KEGGirno:84479.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF121979 mRNA. Translation: ABL63418.1.
EF121977 mRNA. Translation: ABL63416.1.
EF121978 mRNA. Translation: ABL63417.1.
EF121980 mRNA. Translation: ABL63419.1.
EF121981 mRNA. Translation: ABL63420.1.
EF121982 mRNA. Translation: ABL63421.1.
AF242544 mRNA. Translation: AAF61257.1. Different initiation.
AF169549 mRNA. Translation: AAD49733.1.
RefSeqiNP_445871.1. NM_053419.1. [Q9JKC9-1]
UniGeneiRn.122513.

3D structure databases

ProteinModelPortaliQ9JKC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249979. 2 interactions.
MINTiMINT-130277.
STRINGi10116.ENSRNOP00000052759.

PTM databases

iPTMnetiQ9JKC9.

Proteomic databases

PaxDbiQ9JKC9.
PRIDEiQ9JKC9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84479.
KEGGirno:84479.

Organism-specific databases

CTDi11276.
RGDi620684. Synrg.

Phylogenomic databases

eggNOGiKOG0998. Eukaryota.
ENOG410XTDR. LUCA.
HOGENOMiHOG000293152.
HOVERGENiHBG055053.
InParanoidiQ9JKC9.
PhylomeDBiQ9JKC9.

Miscellaneous databases

NextBioi616990.
PROiQ9JKC9.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR000261. EH_dom.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50031. EH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
    Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P., Radecki T., Joe B.
    Genomics 89:343-353(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: Lewis.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-879 (ISOFORMS 2/3), INTERACTION WITH SCAMP1.
  3. "Gamma-synergin: an EH domain-containing protein that interacts with gamma-adaptin."
    Page L.J., Sowerby P.J., Lui W.W.Y., Robinson M.S.
    J. Cell Biol. 146:993-1004(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 165-879 (ISOFORMS 2/3), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-904; SER-997; SER-1088 AND SER-1090, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYNRG_RAT
AccessioniPrimary (citable) accession number: Q9JKC9
Secondary accession number(s): A1EC70
, A1EC72, A1EC74, A1EC75, Q9R145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: February 6, 2007
Last modified: May 11, 2016
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.