Q9JKC8 (AP3M1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-3 complex subunit mu-1

Alternative name(s):
AP-3 adapter complex mu3A subunit
Adapter-related protein complex 3 mu-1 subunit
Mu-adaptin 3A
Mu3A-adaptin

Gene names

Name:

Ap3m1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. Ref.4
Subunit structure	Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with AGAP1. AP-3 associates with the BLOC-1 complex. Ref.3
Subcellular location	Golgi apparatus.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterograde axon cargo transport Inferred from mutant phenotype Ref.4. Source: UniProtKB anterograde synaptic vesicle transport Inferred from mutant phenotype Ref.4. Source: UniProtKB intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin adaptor complex Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	AP-3 complex subunit mu-1		PRO_0000193782
Regions
	Domain	173 – 418	246	MHD

Sequences

	Sequence		Length	Mass (Da)
	Q9JKC8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 69EC322D820C47D3	FASTA	418	46,936
	10 20 30 40 50 60 MIHSLFLINC SGDIFLEKHW KSVVSQSVCD YFFEAQEKAA DVENVPPVIS TPHHYLISIY 70 80 90 100 110 120 RDKLFFVSVI QTEVPPLFVI EFLHRVADTF QDYFGECSEA AIKDNVVIVY ELLEEMLDNG 130 140 150 160 170 180 FPLATESNIL KELIKPPTIL RSVVNSITGS SNVGDTLPTG QLSNIPWRRA GVKYTNNEAY 190 200 210 220 230 240 FDVVEEIDAI IDKSGSTVFA EIQGVIDACI KLSGMPDLSL SFMNPRLLDD VSFHPCIRFK 250 260 270 280 290 300 RWESERVLSF IPPDGNFRLI SYRVSSQNLV AIPVYVKHSI SFKENSSCGR FDITIGPKQN 310 320 330 340 350 360 MGKTIEGITV TVHMPKVVLN MNLTPTQGSY TFDPVTKVLA WDVGKITPQK LPSLKGLVNL 370 380 390 400 410 QSGAPKPEEN PNLNIQFKIQ QLAISGLKVN RLDMYGEKYK PFKGVKYVTK AGKFQVRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse medium chain clathrin adaptor protein mu3A." Werner H., Nave K.-A. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland and Testis.
[3]	"Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1." Nie Z., Boehm M., Boja E.S., Vass W.C., Bonifacino J.S., Fales H.M., Randazzo P.A. Dev. Cell 5:513-521(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AGAP1.
[4]	"The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse." Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V. Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF242857 mRNA. Translation: AAF63512.1. BC024595 mRNA. Translation: AAH24595.1. BC090983 mRNA. Translation: AAH90983.1.
IPI	IPI00311387.
RefSeq	NP_061299.3. NM_018829.4.
UniGene	Mm.376269.
3D structure databases
ProteinModelPortal	Q9JKC8.
SMR	Q9JKC8. Positions 4-418.
ModBase	Search...
PTM databases
PhosphoSite	Q9JKC8.
Proteomic databases
PaxDb	Q9JKC8.
PRIDE	Q9JKC8.
Protocols and materials databases
DNASU	55946.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000154460; ENSMUSP00000117346; ENSMUSG00000021824.
GeneID	55946.
KEGG	mmu:55946.
UCSC	uc007sla.2. mouse.
Organism-specific databases
CTD	26985.
MGI	MGI:1929212. Ap3m1.
Phylogenomic databases
eggNOG	NOG298719.
GeneTree	ENSGT00530000062779.
HOGENOM	HOG000234366.
HOVERGEN	HBG104910.
InParanoid	Q9JKC8.
KO	K12398.
OMA	HMPKAVL.
Enzyme and pathway databases
Reactome	REACT_93132. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q9JKC8.
Bgee	Q9JKC8.
CleanEx	MM_AP3M1.
Genevestigator	Q9JKC8.
GermOnline	ENSMUSG00000021824. Mus musculus.
Family and domain databases
InterPro	IPR022775. AP_mu_sigma_su. IPR001392. Clathrin_mu. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. IPR011012. Longin-like_dom. [Graphical view]
Pfam	PF00928. Adap_comp_sub. 1 hit. PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF005992. Clathrin_mu. 1 hit.
PRINTS	PR00314. CLATHRINADPT.
SUPFAM	SSF49447. AP50. 1 hit. SSF64356. Longin_like. 1 hit.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. 1 hit. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AP3M1. mouse.
NextBio	311634.
SOURCE	Search...

Entry information

Entry name

AP3M1_MOUSE

Accession

Primary (citable) accession number: Q9JKC8
Secondary accession number(s): Q5BKQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program