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Q9JKC8 (AP3M1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-3 complex subunit mu-1
Alternative name(s):
AP-3 adapter complex mu3A subunit
Adapter-related protein complex 3 subunit mu-1
Mu-adaptin 3A
Mu3A-adaptin
Gene names
Name:Ap3m1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. Ref.4

Subunit structure

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with AGAP1. AP-3 associates with the BLOC-1 complex. Ref.3

Subcellular location

Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.

Sequence similarities

Belongs to the adaptor complexes medium subunit family.

Contains 1 MHD (mu homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418AP-3 complex subunit mu-1
PRO_0000193782

Regions

Domain176 – 417242MHD

Sequences

Sequence LengthMass (Da)Tools
Q9JKC8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 69EC322D820C47D3

FASTA41846,936
        10         20         30         40         50         60 
MIHSLFLINC SGDIFLEKHW KSVVSQSVCD YFFEAQEKAA DVENVPPVIS TPHHYLISIY 

        70         80         90        100        110        120 
RDKLFFVSVI QTEVPPLFVI EFLHRVADTF QDYFGECSEA AIKDNVVIVY ELLEEMLDNG 

       130        140        150        160        170        180 
FPLATESNIL KELIKPPTIL RSVVNSITGS SNVGDTLPTG QLSNIPWRRA GVKYTNNEAY 

       190        200        210        220        230        240 
FDVVEEIDAI IDKSGSTVFA EIQGVIDACI KLSGMPDLSL SFMNPRLLDD VSFHPCIRFK 

       250        260        270        280        290        300 
RWESERVLSF IPPDGNFRLI SYRVSSQNLV AIPVYVKHSI SFKENSSCGR FDITIGPKQN 

       310        320        330        340        350        360 
MGKTIEGITV TVHMPKVVLN MNLTPTQGSY TFDPVTKVLA WDVGKITPQK LPSLKGLVNL 

       370        380        390        400        410 
QSGAPKPEEN PNLNIQFKIQ QLAISGLKVN RLDMYGEKYK PFKGVKYVTK AGKFQVRT 

« Hide

References

« Hide 'large scale' references
[1]"Mouse medium chain clathrin adaptor protein mu3A."
Werner H., Nave K.-A.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland and Testis.
[3]"Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1."
Nie Z., Boehm M., Boja E.S., Vass W.C., Bonifacino J.S., Fales H.M., Randazzo P.A.
Dev. Cell 5:513-521(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AGAP1.
[4]"The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF242857 mRNA. Translation: AAF63512.1.
BC024595 mRNA. Translation: AAH24595.1.
BC090983 mRNA. Translation: AAH90983.1.
RefSeqNP_061299.3. NM_018829.4.
XP_006519355.1. XM_006519292.1.
XP_006519356.1. XM_006519293.1.
UniGeneMm.376269.

3D structure databases

ProteinModelPortalQ9JKC8.
SMRQ9JKC8. Positions 4-418.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9JKC8.

Proteomic databases

PaxDbQ9JKC8.
PRIDEQ9JKC8.

Protocols and materials databases

DNASU55946.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000154460; ENSMUSP00000117346; ENSMUSG00000021824.
GeneID55946.
KEGGmmu:55946.
UCSCuc007sla.2. mouse.

Organism-specific databases

CTD26985.
MGIMGI:1929212. Ap3m1.

Phylogenomic databases

eggNOGNOG298719.
GeneTreeENSGT00530000062779.
HOGENOMHOG000234366.
HOVERGENHBG104910.
InParanoidQ9JKC8.
KOK12398.
OMAHMPKAVL.
OrthoDBEOG79KPF6.
TreeFamTF315187.

Enzyme and pathway databases

ReactomeREACT_93132. Metabolism of proteins.

Gene expression databases

ArrayExpressQ9JKC8.
BgeeQ9JKC8.
CleanExMM_AP3M1.
GenevestigatorQ9JKC8.

Family and domain databases

InterProIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF005992. Clathrin_mu. 1 hit.
PRINTSPR00314. CLATHRINADPT.
SUPFAMSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAP3M1. mouse.
NextBio311634.
PROQ9JKC8.
SOURCESearch...

Entry information

Entry nameAP3M1_MOUSE
AccessionPrimary (citable) accession number: Q9JKC8
Secondary accession number(s): Q5BKQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: March 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot