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Protein

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Gene

Hcn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.1 Publication

Enzyme regulationi

Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. cAMP binding promotes tetramerization and formation of an active channel. Compared to other family members, cAMP has less stimulatory effect on HCN1 because part of the molecules already contain bound cAMP and form homotetramers when cAMP levels are low (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi528 – 5314cAMPBy similarity
Nucleotide bindingi538 – 5392cAMPBy similarity
Nucleotide bindingi579 – 5824cAMPBy similarity

GO - Molecular functioni

  • cAMP binding Source: UniProtKB
  • identical protein binding Source: RGD
  • intracellular cAMP activated cation channel activity Source: UniProtKB
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • sodium channel activity Source: UniProtKB-KW
  • voltage-gated potassium channel activity Source: UniProtKB

GO - Biological processi

  • aging Source: RGD
  • cellular response to cAMP Source: UniProtKB
  • cellular response to interferon-beta Source: RGD
  • inflammatory response Source: RGD
  • maternal behavior Source: RGD
  • negative regulation of action potential Source: RGD
  • positive regulation of cation channel activity Source: RGD
  • potassium ion transmembrane transport Source: UniProtKB
  • regulation of membrane depolarization Source: RGD
  • regulation of membrane hyperpolarization Source: RGD
  • response to calcium ion Source: RGD
  • response to cisplatin Source: RGD
  • response to L-glutamate Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Potassium channel, Sodium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Transport

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene namesi
Name:Hcn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620688. Hcn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 135135CytoplasmicSequence analysisAdd
BLAST
Transmembranei136 – 15621Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini157 – 1626ExtracellularSequence analysis
Transmembranei163 – 18321Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini184 – 20825CytoplasmicSequence analysisAdd
BLAST
Transmembranei209 – 22921Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini230 – 2378ExtracellularSequence analysis
Transmembranei238 – 25821Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini259 – 28931CytoplasmicSequence analysisAdd
BLAST
Transmembranei290 – 31021Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini311 – 33323ExtracellularSequence analysisAdd
BLAST
Intramembranei334 – 35522Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Topological domaini356 – 3605ExtracellularSequence analysis
Transmembranei361 – 38121Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini382 – 910529CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical dendrite Source: RGD
  • axon Source: RGD
  • axon terminus Source: RGD
  • basolateral plasma membrane Source: RGD
  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • dendrite Source: RGD
  • dendrite membrane Source: RGD
  • dendritic shaft Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • somatodendritic compartment Source: RGD
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 910910Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1PRO_0000054110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JKB0.
PRIDEiQ9JKB0.

PTM databases

iPTMnetiQ9JKB0.
PhosphoSiteiQ9JKB0.

Expressioni

Tissue specificityi

Highly expressed in cerebral cortex, cerebellum, throughout the hippocampus, in medial habenula, anterior dorsal nucleus in the thalamus, tenia tecta, several nuclei of the general motor system and in optic nerve layer. Detected in a subset of elongated cells in taste buds.2 Publications

Interactioni

Subunit structurei

Homotetramer. Heterotetramer with HCN2. The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits. Interacts with KCNE2. Interacts with the SH3 domain of CSK (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi249933. 2 interactions.
STRINGi10116.ENSRNOP00000016142.

Structurei

3D structure databases

ProteinModelPortaliQ9JKB0.
SMRiQ9JKB0. Positions 390-590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 12952Involved in subunit assemblyBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi715 – 77763Gln-richAdd
BLAST
Compositional biasi878 – 8847Poly-Pro

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Belongs to the potassium channel HCN family.Curated
Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000230717.
HOVERGENiHBG039489.
InParanoidiQ9JKB0.
KOiK04954.
PhylomeDBiQ9JKB0.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR013621. Ion_trans_N.
IPR030169. K/Na_HCN1.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF373. PTHR10217:SF373. 2 hits.
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08412. Ion_trans_N. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGGGKPNSA SNSRDDGNSV YPSKAPATGP AAADKRLGTP PGGGAAGKEH
60 70 80 90 100
GNSVCFKVDG GGGEEPAGSF EDAEGPRRQY GFMQRQFTSM LQPGVNKFSL
110 120 130 140 150
RMFGSQKAVE KEQERVKTAG FWIIHPYSDF RFYWDLIMLI MMVGNLVIIP
160 170 180 190 200
VGITFFTEQT TTPWIIFNVA SDTVFLLDLI MNFRTGTVNE DSSEIILDPK
210 220 230 240 250
VIKMNYLKSW FVVDFISSIP VDYIFLIVEK GMDSEVYKTA RALRIVRFTK
260 270 280 290 300
ILSLLRLLRL SRLIRYIHQW EEIFHMTYDL ASAVVRIFNL IGMMLLLCHW
310 320 330 340 350
DGCLQFLVPL LQDFPPDCWV SLNEMVNDSW GKQYSYALFK AMSHMLCIGY
360 370 380 390 400
GAQAPVSMSD LWITMLSMIV GATCYAMFVG HATALIQSLD SSRRQYQEKY
410 420 430 440 450
KQVEQYMSFH KLPADMRQKI HDYYEHRYQG KIFDEENILS ELNDPLREEI
460 470 480 490 500
VNFNCRKLVA TMPLFANADP NFVTAMLSKL RFEVFQPGDY IIREGAVGKK
510 520 530 540 550
MYFIQHGVAG VITKSSKEMK LTDGSYFGEI CLLTKGRRTA SVRADTYCRL
560 570 580 590 600
YSLSVDNFNE VLEEYPMMRR AFETVAIDRL DRIGKKNSIL LQKFQKDLNT
610 620 630 640 650
GVFNNQENEI LKQIVKHDRE MVQAIPPINY PQMTALNCTS STTTPTSRMR
660 670 680 690 700
TQSPPVYTAT SLSHSNLHSP SPSTQTPQPS AILSPCSYTT AVCSPPIQSP
710 720 730 740 750
LATRTFHYAS PTASQLSLMQ QPQPQLQQSQ VQQTQTQTQQ QQQQQQPQPQ
760 770 780 790 800
PQQPQQQQQQ QQQQQQQQQQ QQQQQPQTPG SSTPKNEVHK STQALHNTHL
810 820 830 840 850
TREVRPLSAS QPSLPHEVST MISRPHPTVG ESLASIPQPV ATVHSTGLQA
860 870 880 890 900
GSRSTVPQRV TLFRQMSSGA IPPNRGVPPA PPPPAAVQRE SPSVLNKDPD
910
AEKPRFASNL
Length:910
Mass (Da):102,422
Last modified:October 1, 2000 - v1
Checksum:i11E32D2BE73F4528
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247450 mRNA. Translation: AAF62173.1.
RefSeqiNP_445827.1. NM_053375.1.
UniGeneiRn.21408.

Genome annotation databases

GeneIDi84390.
KEGGirno:84390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247450 mRNA. Translation: AAF62173.1.
RefSeqiNP_445827.1. NM_053375.1.
UniGeneiRn.21408.

3D structure databases

ProteinModelPortaliQ9JKB0.
SMRiQ9JKB0. Positions 390-590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249933. 2 interactions.
STRINGi10116.ENSRNOP00000016142.

PTM databases

iPTMnetiQ9JKB0.
PhosphoSiteiQ9JKB0.

Proteomic databases

PaxDbiQ9JKB0.
PRIDEiQ9JKB0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84390.
KEGGirno:84390.

Organism-specific databases

CTDi348980.
RGDi620688. Hcn1.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000230717.
HOVERGENiHBG039489.
InParanoidiQ9JKB0.
KOiK04954.
PhylomeDBiQ9JKB0.

Miscellaneous databases

PROiQ9JKB0.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR013621. Ion_trans_N.
IPR030169. K/Na_HCN1.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF373. PTHR10217:SF373. 2 hits.
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08412. Ion_trans_N. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCN1_RAT
AccessioniPrimary (citable) accession number: Q9JKB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.