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Protein

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Gene

Hcn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.1 Publication

Enzyme regulationi

Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. cAMP binding promotes tetramerization and formation of an active channel. Compared to other family members, cAMP has less stimulatory effect on HCN1 because part of the molecules already contain bound cAMP and form homotetramers when cAMP levels are low (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi528 – 531cAMPBy similarity4
Nucleotide bindingi538 – 539cAMPBy similarity2
Nucleotide bindingi579 – 582cAMPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to cAMP Source: UniProtKB
  • cellular response to interferon-beta Source: RGD
  • inflammatory response Source: RGD
  • maternal behavior Source: RGD
  • negative regulation of action potential Source: RGD
  • positive regulation of cation channel activity Source: RGD
  • potassium ion transmembrane transport Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
  • regulation of membrane depolarization Source: RGD
  • regulation of membrane hyperpolarization Source: RGD
  • response to calcium ion Source: RGD
  • response to cisplatin Source: RGD
  • response to L-glutamate Source: RGD

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Potassium channel, Sodium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Sodium transport, Transport
LigandcAMP, cAMP-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene namesi
Name:Hcn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620688 Hcn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 131CytoplasmicBy similarityAdd BLAST131
Transmembranei132 – 153Helical; Name=Segment S1By similarityAdd BLAST22
Topological domaini154 – 162ExtracellularBy similarity9
Transmembranei163 – 183Helical; Name=Segment S2By similarityAdd BLAST21
Topological domaini184 – 204CytoplasmicBy similarityAdd BLAST21
Transmembranei205 – 225Helical; Name=Segment S3By similarityAdd BLAST21
Topological domaini226 – 249ExtracellularBy similarityAdd BLAST24
Transmembranei250 – 270Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST21
Topological domaini271 – 284CytoplasmicBy similarityAdd BLAST14
Transmembranei285 – 307Helical; Name=Segment S5By similarityAdd BLAST23
Topological domaini308 – 333ExtracellularBy similarityAdd BLAST26
Intramembranei334 – 355Pore-forming; Name=Segment H5By similarityAdd BLAST22
Topological domaini356 – 360ExtracellularBy similarity5
Transmembranei361 – 381Helical; Name=Segment S6By similarityAdd BLAST21
Topological domaini382 – 910CytoplasmicBy similarityAdd BLAST529

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541101 – 910Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1Add BLAST910

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi327N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JKB0
PRIDEiQ9JKB0

PTM databases

iPTMnetiQ9JKB0
PhosphoSitePlusiQ9JKB0

Expressioni

Tissue specificityi

Highly expressed in cerebral cortex, cerebellum, throughout the hippocampus, in medial habenula, anterior dorsal nucleus in the thalamus, tenia tecta, several nuclei of the general motor system and in optic nerve layer. Detected in a subset of elongated cells in taste buds.2 Publications

Interactioni

Subunit structurei

Homotetramer. Heterotetramer with HCN2. The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits. Interacts with KCNE2. Interacts with the SH3 domain of CSK.By similarity

GO - Molecular functioni

  • identical protein binding Source: RGD

Protein-protein interaction databases

BioGridi249933, 2 interactors
STRINGi10116.ENSRNOP00000016142

Structurei

3D structure databases

ProteinModelPortaliQ9JKB0
SMRiQ9JKB0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 82Intrinsically disorderedCuratedAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi347 – 351Selectivity filterBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi715 – 777Gln-richAdd BLAST63
Compositional biasi878 – 884Poly-Pro7

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.By similarity

Sequence similaritiesi

Belongs to the potassium channel HCN family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOGENOMiHOG000230717
HOVERGENiHBG039489
InParanoidiQ9JKB0
KOiK04954
PhylomeDBiQ9JKB0

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
Gene3Di1.20.120.350, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR013621 Ion_trans_N
IPR030169 K/Na_HCN1
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR014710 RmlC-like_jellyroll
IPR027359 Volt_channel_dom_sf
PANTHERiPTHR10217:SF373 PTHR10217:SF373, 1 hit
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF08412 Ion_trans_N, 1 hit
PRINTSiPR01463 EAGCHANLFMLY
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SUPFAMiSSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 1 hit
PS50042 CNMP_BINDING_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JKB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGGGKPNSA SNSRDDGNSV YPSKAPATGP AAADKRLGTP PGGGAAGKEH
60 70 80 90 100
GNSVCFKVDG GGGEEPAGSF EDAEGPRRQY GFMQRQFTSM LQPGVNKFSL
110 120 130 140 150
RMFGSQKAVE KEQERVKTAG FWIIHPYSDF RFYWDLIMLI MMVGNLVIIP
160 170 180 190 200
VGITFFTEQT TTPWIIFNVA SDTVFLLDLI MNFRTGTVNE DSSEIILDPK
210 220 230 240 250
VIKMNYLKSW FVVDFISSIP VDYIFLIVEK GMDSEVYKTA RALRIVRFTK
260 270 280 290 300
ILSLLRLLRL SRLIRYIHQW EEIFHMTYDL ASAVVRIFNL IGMMLLLCHW
310 320 330 340 350
DGCLQFLVPL LQDFPPDCWV SLNEMVNDSW GKQYSYALFK AMSHMLCIGY
360 370 380 390 400
GAQAPVSMSD LWITMLSMIV GATCYAMFVG HATALIQSLD SSRRQYQEKY
410 420 430 440 450
KQVEQYMSFH KLPADMRQKI HDYYEHRYQG KIFDEENILS ELNDPLREEI
460 470 480 490 500
VNFNCRKLVA TMPLFANADP NFVTAMLSKL RFEVFQPGDY IIREGAVGKK
510 520 530 540 550
MYFIQHGVAG VITKSSKEMK LTDGSYFGEI CLLTKGRRTA SVRADTYCRL
560 570 580 590 600
YSLSVDNFNE VLEEYPMMRR AFETVAIDRL DRIGKKNSIL LQKFQKDLNT
610 620 630 640 650
GVFNNQENEI LKQIVKHDRE MVQAIPPINY PQMTALNCTS STTTPTSRMR
660 670 680 690 700
TQSPPVYTAT SLSHSNLHSP SPSTQTPQPS AILSPCSYTT AVCSPPIQSP
710 720 730 740 750
LATRTFHYAS PTASQLSLMQ QPQPQLQQSQ VQQTQTQTQQ QQQQQQPQPQ
760 770 780 790 800
PQQPQQQQQQ QQQQQQQQQQ QQQQQPQTPG SSTPKNEVHK STQALHNTHL
810 820 830 840 850
TREVRPLSAS QPSLPHEVST MISRPHPTVG ESLASIPQPV ATVHSTGLQA
860 870 880 890 900
GSRSTVPQRV TLFRQMSSGA IPPNRGVPPA PPPPAAVQRE SPSVLNKDPD
910
AEKPRFASNL
Length:910
Mass (Da):102,422
Last modified:October 1, 2000 - v1
Checksum:i11E32D2BE73F4528
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247450 mRNA Translation: AAF62173.1
RefSeqiNP_445827.1, NM_053375.1
UniGeneiRn.21408

Genome annotation databases

GeneIDi84390
KEGGirno:84390

Similar proteinsi

Entry informationi

Entry nameiHCN1_RAT
AccessioniPrimary (citable) accession number: Q9JKB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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