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Protein

Partitioning defective 6 homolog beta

Gene

Pard6b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-MMU-420029. Tight junction interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective 6 homolog beta
Short name:
PAR-6 beta
Short name:
PAR-6B
Gene namesi
Name:Pard6b
Synonyms:Par6b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2135605. Pard6b.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • apical plasma membrane Source: MGI
  • bicellular tight junction Source: UniProtKB
  • cell cortex Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: UniProtKB
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi133 – 1342IS → AA: Does not abolish interaction with CDC42 and ARHQ. 1 Publication
Mutagenesisi136 – 1361P → A: Abolishes interaction with ARHQ, but not the interaction with CDC42; when associated with A-139. 1 Publication
Mutagenesisi139 – 1391F → A: Abolishes interaction with ARHQ, but not the interaction with CDC42; when associated with A-136. 1 Publication
Mutagenesisi167 – 1704KPLG → AAAA: Strongly reduces interaction with PARD3. Does not abolish interaction with CDC42 and ARHQ. 1 Publication
Mutagenesisi235 – 2351M → W: Prevents interaction with MPP5. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371Partitioning defective 6 homolog betaPRO_0000112517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei11 – 111PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JK83.
PaxDbiQ9JK83.
PeptideAtlasiQ9JK83.
PRIDEiQ9JK83.

PTM databases

iPTMnetiQ9JK83.
PhosphoSiteiQ9JK83.

Expressioni

Tissue specificityi

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Gene expression databases

BgeeiENSMUSG00000044641.
GenevisibleiQ9JK83. MM.

Interactioni

Subunit structurei

Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with ALS2CR19. Interacts with ECT2 (By similarity). Interacts with MPP5.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P609536EBI-81861,EBI-81752From a different organism.
PARD3Q8TEW03EBI-81861,EBI-81968From a different organism.

Protein-protein interaction databases

BioGridi208398. 12 interactions.
IntActiQ9JK83. 17 interactions.
MINTiMINT-4106590.
STRINGi10090.ENSMUSP00000052619.

Structurei

Secondary structure

1
371
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi137 – 14610Combined sources
Beta strandi156 – 1605Combined sources
Beta strandi171 – 18212Combined sources
Beta strandi185 – 19713Combined sources
Helixi202 – 2065Combined sources
Beta strandi214 – 2185Combined sources
Beta strandi221 – 2233Combined sources
Helixi228 – 23710Combined sources
Turni238 – 2403Combined sources
Beta strandi241 – 2477Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NF3X-ray2.10C/D126-253[»]
ProteinModelPortaliQ9JK83.
SMRiQ9JK83. Positions 17-96, 131-253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JK83.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9681PB1PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 15018Pseudo-CRIBAdd
BLAST
Domaini157 – 25094PDZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 253128Interaction with PARD3 and CDC42Add
BLAST

Domaini

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.1 Publication
The PDZ domain mediates the interaction with MPP5.1 Publication

Sequence similaritiesi

Belongs to the PAR6 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 pseudo-CRIB domain.Curated

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9JK83.
KOiK06093.
OMAiIEDSGEP.
OrthoDBiEOG091G0FFH.
TreeFamiTF312899.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JK83-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRGHRHGAS SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV
60 70 80 90 100
HKIPNVDVLV GYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD
110 120 130 140 150
YSAFGTDTLI RKKNMLSNVL RPDNHRKKPH IVISMPQDFR PVSSIIDVDI
160 170 180 190 200
LPETHRRVRL YKYGTEKPLG FYIRDGSSVR VTPHGLEKVP GIFISRLVPG
210 220 230 240 250
GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSR NLIITVRPAN
260 270 280 290 300
QRNNVVRNSR TSGSSSQSTD NSLLGFPQQV EASFEPEDQD SDEDDIIIED
310 320 330 340 350
SGEPQQIPKA TPAQSLESLT QIELSFESGQ NGFSPPQDTS LVPVPGSLDT
360 370
ELESRAPDQK LLEEDGTIIT L
Length:371
Mass (Da):41,067
Last modified:July 27, 2011 - v2
Checksum:i664C7D25262494A9
GO
Isoform 2 (identifier: Q9JK83-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-298: I → C
     299-371: Missing.

Note: No experimental confirmation available.
Show »
Length:298
Mass (Da):33,267
Checksum:i842E7B79CE012070
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131C → W in AAH25147 (PubMed:15489334).Curated
Sequence conflicti70 – 701L → P in AAF71528 (PubMed:10934474).Curated
Sequence conflicti161 – 1611Y → C in AAF71528 (PubMed:10934474).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei298 – 2981I → C in isoform 2. 1 PublicationVSP_007460
Alternative sequencei299 – 37173Missing in isoform 2. 1 PublicationVSP_007461Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252291 mRNA. Translation: AAF71528.1.
AL831766 Genomic DNA. Translation: CAM17536.1.
CH466551 Genomic DNA. Translation: EDL06536.1.
BC025147 mRNA. Translation: AAH25147.1.
CCDSiCCDS17108.1. [Q9JK83-1]
RefSeqiNP_067384.2. NM_021409.2. [Q9JK83-1]
UniGeneiMm.292834.

Genome annotation databases

EnsembliENSMUST00000052125; ENSMUSP00000052619; ENSMUSG00000044641. [Q9JK83-1]
GeneIDi58220.
KEGGimmu:58220.
UCSCiuc008oao.2. mouse. [Q9JK83-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252291 mRNA. Translation: AAF71528.1.
AL831766 Genomic DNA. Translation: CAM17536.1.
CH466551 Genomic DNA. Translation: EDL06536.1.
BC025147 mRNA. Translation: AAH25147.1.
CCDSiCCDS17108.1. [Q9JK83-1]
RefSeqiNP_067384.2. NM_021409.2. [Q9JK83-1]
UniGeneiMm.292834.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NF3X-ray2.10C/D126-253[»]
ProteinModelPortaliQ9JK83.
SMRiQ9JK83. Positions 17-96, 131-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208398. 12 interactions.
IntActiQ9JK83. 17 interactions.
MINTiMINT-4106590.
STRINGi10090.ENSMUSP00000052619.

PTM databases

iPTMnetiQ9JK83.
PhosphoSiteiQ9JK83.

Proteomic databases

MaxQBiQ9JK83.
PaxDbiQ9JK83.
PeptideAtlasiQ9JK83.
PRIDEiQ9JK83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052125; ENSMUSP00000052619; ENSMUSG00000044641. [Q9JK83-1]
GeneIDi58220.
KEGGimmu:58220.
UCSCiuc008oao.2. mouse. [Q9JK83-1]

Organism-specific databases

CTDi84612.
MGIiMGI:2135605. Pard6b.

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9JK83.
KOiK06093.
OMAiIEDSGEP.
OrthoDBiEOG091G0FFH.
TreeFamiTF312899.

Enzyme and pathway databases

ReactomeiR-MMU-420029. Tight junction interactions.

Miscellaneous databases

EvolutionaryTraceiQ9JK83.
PROiQ9JK83.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044641.
GenevisibleiQ9JK83. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAR6B_MOUSE
AccessioniPrimary (citable) accession number: Q9JK83
Secondary accession number(s): A2ANX8, Q8R3J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.