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Q9JK82 (EXT1_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exostosin-1

EC=2.4.1.224
EC=2.4.1.225
Alternative name(s):
Heparan sulfate copolymerase
Multiple exostoses protein 1 homolog
Gene names
Name:EXT1
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.

UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactor

Divalent cations.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Forms a homo/hetero-oligomeric complex with EXT2 By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Note: The EXT1/EXT2 complex is localized in the Golgi apparatus By similarity.

Sequence similarities

Belongs to the glycosyltransferase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 746746Exostosin-1
PRO_0000149647

Regions

Topological domain1 – 55Cytoplasmic Potential
Transmembrane6 – 2621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain27 – 746720Lumenal Potential

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation3301N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2681G → E: Reduced GlcA transferase activity.
Mutagenesis2981C → Y: Reduced GlcA transferase activity.
Mutagenesis3411R → K: Reduced GlcA transferase activity.
Mutagenesis3441S → F: Reduced GlcA transferase activity.
Mutagenesis3461R → K: Reduced GlcA transferase activity.
Mutagenesis3491E → K: Reduced GlcA transferase activity.

Sequences

Sequence LengthMass (Da)Tools
Q9JK82 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C3697B4A421DA4F2

FASTA74686,189
        10         20         30         40         50         60 
MQAKKRYFIL LSAGSCLALL FYFGGVQFRA SRSHSRREEH SGRNGLHQPS PDHFWPRFAD 

        70         80         90        100        110        120 
ALHPFFPWDQ LENEDSGVHV SPRQKRDANS SVYKGKKCRM ESCFDFALCK KNGFKVYVYP 

       130        140        150        160        170        180 
QQKGEKIAES YQNILAAIEG SRFYTSDPSQ ACLFVLSLDT LDRDQLSPQY VHNLRSKVQS 

       190        200        210        220        230        240 
LHLWNNGRNH LIFNLYSGTW PDYTEDVGFD IGQAMLAKAS ISTENFRPNF DVSIPLFSKD 

       250        260        270        280        290        300 
HPRTGGERGF LKFNTIPPLR KYMLVFKGKR YLTGIGSDTR NALYHVHNGE DVLLLTTCKH 

       310        320        330        340        350        360 
GKDWQKHKDS RCDRDNTEYE KYDYREMLHN ATFCLVPRGR RLGSFRFLEA LQAACVPVML 

       370        380        390        400        410        420 
SNGWELPFSE VINWNQAAVI GDERLLLQIP STIRSIHQDK ILALRQQTQF LWEAYFSSVE 

       430        440        450        460        470        480 
KIVLTTLEII QDRIFKHISR NSLIWNKHPG GLFVLPQYSS YLGDFPYYYA NLGLKPPSKF 

       490        500        510        520        530        540 
TAVIHAVTPL VSQSQPVLKL LVAAAKSQYC AQIIVLWNCD KPLPAKHRWP ATAVPVIVIE 

       550        560        570        580        590        600 
GESKVMSSRF LPYDNIITDA VLSLDEDTVL STTEVDFAFT VWQSFPERIV GYPARSHFWD 

       610        620        630        640        650        660 
NSKERWGYTS KWTNDYSMVL TGAAIYHKYY HYLYTHYLPA SLKNMVDQLA NCEDILMNFL 

       670        680        690        700        710        720 
VSAVTKLPPI KVTQKKQYKE TMMGQTSRAS RWADPDHFAQ RQSCMNTFAS WFGYMPLIHS 

       730        740 
QMRLDPVLFK DQVSILRKKY RDIERL 

« Hide

References

[1]"Location of the glucuronosyltransferase domain in the heparan sulfate copolymerase EXT1 by analysis of Chinese hamster ovary cell mutants."
Wei G., Bai X., Gabb M.M.G., Bame K.J., Koshy T.I., Spear P.G., Esko J.D.
J. Biol. Chem. 275:27733-27740(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION OF MUTANTS GLY-268; CYS-298; ARG-341; SER-344; ARG-346 AND GLU-349.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF252858 mRNA. Translation: AAF71276.1.
RefSeqNP_001233696.1. NM_001246767.1.

3D structure databases

ProteinModelPortalQ9JK82.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100689334.
KEGGcge:100689334.

Organism-specific databases

CTD2131.

Phylogenomic databases

HOVERGENHBG003459.
KOK02366.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF45. PTHR11062:SF45. 1 hit.
PfamPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Entry information

Entry nameEXT1_CRIGR
AccessionPrimary (citable) accession number: Q9JK82
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways