Q9JK71 (MAGI3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Alternative name(s):
Membrane-associated guanylate kinase inverted 3
Short name=MAGI-3
Scaffolding-like protein

Gene names

Name:	Magi3
Synonyms:	Slipr

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1470 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. Ref.1
Subunit structure	Interacts with ADRB1, BAI1, LPAR2/EDG4, FZD4, FZD7, GRIN2B, TGFA and VANGL2 By similarity. Interacts with PTEN. Interacts with ADRB1, PTPRB and unidentified tyrosine phosphorylated proteins. Ref.1 Ref.2 Ref.3
Subcellular location	Cell membrane; Peripheral membrane protein. Cell junction › tight junction. Nucleus. Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions. Ref.1 Ref.3
Sequence similarities	Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 6 PDZ (DHR) domains. Contains 2 WW domains.
Sequence caution	The sequence AAF66069.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Nucleus Tight junction
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell tight junction Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Adrb1	P18090	3	EBI-696226,EBI-991303
PTEN	P60484	3	EBI-696226,EBI-696162	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1470	1470	Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3		PRO_0000341409
Regions
	Domain	18 – 108	91	PDZ 1
	Domain	116 – 290	175	Guanylate kinase-like
	Domain	296 – 329	34	WW 1
	Domain	342 – 375	34	WW 2
	Domain	413 – 495	83	PDZ 2
	Domain	581 – 657	77	PDZ 3
	Domain	729 – 811	83	PDZ 4
	Domain	852 – 939	88	PDZ 5
	Domain	1022 – 1104	83	PDZ 6
	Nucleotide binding	123 – 130	8	ATP By similarity
	Region	18 – 108	91	Interaction with ADRB1 and TGFA
	Region	413 – 495	83	Interaction with PTEN By similarity
	Region	729 – 811	83	Interaction with BAI1 By similarity
	Region	852 – 939	88	Interaction with LPAR2 and GRIN2B By similarity
	Compositional bias	6 – 9	4	Poly-Lys
	Compositional bias	240 – 245	6	Poly-Glu
Amino acid modifications
	Modified residue	700	1	Phosphoserine By similarity
	Modified residue	702	1	Phosphoserine By similarity
	Modified residue	833	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JK71 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 70E2AFFCE5EA8480	FASTA	1,470	160,563
	10 20 30 40 50 60 MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD EPGGGGGTCC 70 80 90 100 110 120 VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI RLKTVKPGKV INKDLRHYLS 130 140 150 160 170 180 LQFQKGSIDH KLQQVIRDNL YLITIPCTTR APRDGEVPGV DYNFISVEQF KALEESGALL 190 200 210 220 230 240 ESGTYDGNFY GTPKPPAEPS PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE 250 260 270 280 290 300 EEEDEDKEAV NGSGSMETRE MHSESSDCWM KTVPSYNQTN RSMDFRNYMM RDENLEPLPK 310 320 330 340 350 360 NWEMAYTDTG TIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK IEDPQYGTYY 370 380 390 400 410 420 VDHLNQKTQF ENPVEEAKRK KQIGQAETHS AKTDVERAHF TRDPSQLKGV LVRASLKKST 430 440 450 460 470 480 MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ DGKMAPGDVI VDINGNCVLG HTHADVVQMF 490 500 510 520 530 540 QLVPVNQYVN LTLCRGYALP DDSEDPVVDI VAATPVINGQ SLAKGEACMS TQDFKLGAMV 550 560 570 580 590 600 LDQNGKSGKL LSSDRLNGPS DSNEQRASLA SSGSSQPELV TIPLVKGPKG FGFAIADSPT 610 620 630 640 650 660 GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD VPLLILRGGP 670 680 690 700 710 720 CSPTKTAKMK TDTKETSGSL ETINEPTPQP MPFPPSIIRS GSPKLDPSEV YLKSKTLYED 730 740 750 760 770 780 KPPNTKDLDV FLRKQESGFG FRVLGGDGPD QSIYIGAIIP LGAAEKDGRL RAADELMCID 790 800 810 820 830 840 GIPVKGKSHK QVLDLMTTAA RNGHVLLTVR RKIFYGEKQP EDESPQAFSQ SGSPRLNRTE 850 860 870 880 890 900 LPTRSAPQES YDVILQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGRLKV 910 920 930 940 950 960 GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS ARQSPALQHR 970 980 990 1000 1010 1020 PMGQAQATHI PGDRTALEGE VGKDVCSSYR HSWSDHKHLA QPDTAVISVV GSRHSQSLGC 1030 1040 1050 1060 1070 1080 YPVELERGPR GFGFSLRGGK EYNMGLFILR LAEDGPAIKD GRIHVGDQIV EINGEPTQGI 1090 1100 1110 1120 1130 1140 THTRAIELIQ AGGNKVLLLL RPGTGLIPDH GDWDIYSPSS SNVIYDEQPP PLPSSHSAAT 1150 1160 1170 1180 1190 1200 FEESHVPVTE DSLIRVQTCE KAEELKDTVQ EKKSTLNGSQ PEMKYQSIQK NVSKKDPSRS 1210 1220 1230 1240 1250 1260 HGHGDKNLLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLRSDPKGK SRDRSLSPRK 1270 1280 1290 1300 1310 1320 GENKGQVTIK AGSGQDPCRK DRGRSSSPRK QQKIGGNSLS NTEGKLSEAG SRRAAGLSSD 1330 1340 1350 1360 1370 1380 SPEQLPEGKE KSGVSRKDLK LSQLGKNRTR SPEKRSSKVD EASLPSKKTS DTASRVVSEK 1390 1400 1410 1420 1430 1440 EKGRKPGTGE RSRDKTGESV QTSAKPLTQE AGEKMALSKA SEVTDRGKER AGGAPESSSP 1450 1460 1470 VKKAPITPGP WRVPRANKVT GTAGMADKQL

References

[1]	"Junctional protein MAGI-3 interacts with receptor tyrosine phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins." Adamsky K., Arnold K., Sabanay H., Peles E. J. Cell Sci. 116:1279-1289(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PTPRB.
[2]	"Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases." Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R. J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PTEN.
[3]	"Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins." He J., Bellini M., Inuzuka H., Xu J., Xiong Y., Yang X., Castleberry A.M., Hall R.A. J. Biol. Chem. 281:2820-2827(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ADRB1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF255614 mRNA. Translation: AAF66069.1. Frameshift.
IPI	IPI00202137.
RefSeq	NP_620784.2. NM_139084.2.
UniGene	Rn.228785.
3D structure databases
HSSP	HSSP built from PDB template 1UEQ based on UniProtKB Q86UL8.
ProteinModelPortal	Q9JK71.
SMR	Q9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JK71. 2 interactions.
STRING	10116.ENSRNOP00000026952.
PTM databases
PhosphoSite	Q9JK71.
Proteomic databases
PaxDb	Q9JK71.
PRIDE	Q9JK71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	245903.
KEGG	rno:245903.
UCSC	RGD:621362. rat.
Organism-specific databases
CTD	260425.
RGD	621362. Magi3.
Phylogenomic databases
eggNOG	COG5021.
HOGENOM	HOG000113463.
HOVERGEN	HBG007091.
InParanoid	Q9JK71.
KO	K06112.
OrthoDB	EOG41RPTC.
Gene expression databases
ArrayExpress	Q9JK71.
Genevestigator	Q9JK71.
Family and domain databases
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR001478. PDZ. IPR001202. WW_dom. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. PF00595. PDZ. 4 hits. PF00397. WW. 2 hits. [Graphical view]
SMART	SM00072. GuKc. 1 hit. SM00228. PDZ. 6 hits. SM00456. WW. 2 hits. [Graphical view]
SUPFAM	SSF50156. PDZ. 6 hits. SSF51045. WW_Rsp5_WWP. 2 hits.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. PS50106. PDZ. 6 hits. PS01159. WW_DOMAIN_1. 2 hits. PS50020. WW_DOMAIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	623144.

Entry information

Entry name

MAGI3_RAT

Accession

Primary (citable) accession number: Q9JK71

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program