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Q9JK71

- MAGI3_RAT

UniProt

Q9JK71 - MAGI3_RAT

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Protein
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Gene
Magi3, Slipr
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1308ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Alternative name(s):
Membrane-associated guanylate kinase inverted 3
Short name:
MAGI-3
Scaffolding-like protein
Gene namesi
Name:Magi3
Synonyms:Slipr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621362. Magi3.

Subcellular locationi

Cell membrane; Peripheral membrane protein. Cell junctiontight junction. Nucleus
Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.2 Publications

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
  3. tight junction Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14701470Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
PRO_0000341409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JK71.
PRIDEiQ9JK71.

PTM databases

PhosphoSiteiQ9JK71.

Expressioni

Gene expression databases

GenevestigatoriQ9JK71.

Interactioni

Subunit structurei

Interacts with ADRB1, BAI1, LPAR2/EDG4, FZD4, FZD7, GRIN2B, TGFA and VANGL2 By similarity. Interacts with PTEN. Interacts with ADRB1, PTPRB and unidentified tyrosine phosphorylated proteins.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Adrb1P180903EBI-696226,EBI-991303
PTENP604843EBI-696226,EBI-696162From a different organism.

Protein-protein interaction databases

BioGridi251439. 3 interactions.
IntActiQ9JK71. 2 interactions.
STRINGi10116.ENSRNOP00000026952.

Structurei

3D structure databases

ProteinModelPortaliQ9JK71.
SMRiQ9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 10891PDZ 1
Add
BLAST
Domaini116 – 290175Guanylate kinase-like
Add
BLAST
Domaini296 – 32934WW 1
Add
BLAST
Domaini342 – 37534WW 2
Add
BLAST
Domaini413 – 49583PDZ 2
Add
BLAST
Domaini581 – 65777PDZ 3
Add
BLAST
Domaini729 – 81183PDZ 4
Add
BLAST
Domaini852 – 93988PDZ 5
Add
BLAST
Domaini1022 – 110483PDZ 6
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 10891Interaction with ADRB1 and TGFA
Add
BLAST
Regioni413 – 49583Interaction with PTEN By similarity
Add
BLAST
Regioni729 – 81183Interaction with BAI1 By similarity
Add
BLAST
Regioni852 – 93988Interaction with LPAR2 and GRIN2B By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 94Poly-Lys
Compositional biasi240 – 2456Poly-Glu

Sequence similaritiesi

Belongs to the MAGUK family.
Contains 6 PDZ (DHR) domains.
Contains 2 WW domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5021.
HOGENOMiHOG000113463.
HOVERGENiHBG007091.
InParanoidiQ9JK71.
KOiK06112.
PhylomeDBiQ9JK71.

Family and domain databases

Gene3Di2.30.42.10. 6 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 4 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JK71-1 [UniParc]FASTAAdd to Basket

« Hide

MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD     50
EPGGGGGTCC VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI 100
RLKTVKPGKV INKDLRHYLS LQFQKGSIDH KLQQVIRDNL YLITIPCTTR 150
APRDGEVPGV DYNFISVEQF KALEESGALL ESGTYDGNFY GTPKPPAEPS 200
PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE EEEDEDKEAV 250
NGSGSMETRE MHSESSDCWM KTVPSYNQTN RSMDFRNYMM RDENLEPLPK 300
NWEMAYTDTG TIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK 350
IEDPQYGTYY VDHLNQKTQF ENPVEEAKRK KQIGQAETHS AKTDVERAHF 400
TRDPSQLKGV LVRASLKKST MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ 450
DGKMAPGDVI VDINGNCVLG HTHADVVQMF QLVPVNQYVN LTLCRGYALP 500
DDSEDPVVDI VAATPVINGQ SLAKGEACMS TQDFKLGAMV LDQNGKSGKL 550
LSSDRLNGPS DSNEQRASLA SSGSSQPELV TIPLVKGPKG FGFAIADSPT 600
GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD 650
VPLLILRGGP CSPTKTAKMK TDTKETSGSL ETINEPTPQP MPFPPSIIRS 700
GSPKLDPSEV YLKSKTLYED KPPNTKDLDV FLRKQESGFG FRVLGGDGPD 750
QSIYIGAIIP LGAAEKDGRL RAADELMCID GIPVKGKSHK QVLDLMTTAA 800
RNGHVLLTVR RKIFYGEKQP EDESPQAFSQ SGSPRLNRTE LPTRSAPQES 850
YDVILQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGRLKV 900
GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS 950
ARQSPALQHR PMGQAQATHI PGDRTALEGE VGKDVCSSYR HSWSDHKHLA 1000
QPDTAVISVV GSRHSQSLGC YPVELERGPR GFGFSLRGGK EYNMGLFILR 1050
LAEDGPAIKD GRIHVGDQIV EINGEPTQGI THTRAIELIQ AGGNKVLLLL 1100
RPGTGLIPDH GDWDIYSPSS SNVIYDEQPP PLPSSHSAAT FEESHVPVTE 1150
DSLIRVQTCE KAEELKDTVQ EKKSTLNGSQ PEMKYQSIQK NVSKKDPSRS 1200
HGHGDKNLLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLRSDPKGK 1250
SRDRSLSPRK GENKGQVTIK AGSGQDPCRK DRGRSSSPRK QQKIGGNSLS 1300
NTEGKLSEAG SRRAAGLSSD SPEQLPEGKE KSGVSRKDLK LSQLGKNRTR 1350
SPEKRSSKVD EASLPSKKTS DTASRVVSEK EKGRKPGTGE RSRDKTGESV 1400
QTSAKPLTQE AGEKMALSKA SEVTDRGKER AGGAPESSSP VKKAPITPGP 1450
WRVPRANKVT GTAGMADKQL 1470
Length:1,470
Mass (Da):160,563
Last modified:June 10, 2008 - v2
Checksum:i70E2AFFCE5EA8480
GO

Sequence cautioni

The sequence AAF66069.1 differs from that shown. Reason: Frameshift at several positions.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF255614 mRNA. Translation: AAF66069.1. Frameshift.
RefSeqiNP_620784.2. NM_139084.2.
UniGeneiRn.228785.

Genome annotation databases

GeneIDi245903.
KEGGirno:245903.
UCSCiRGD:621362. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF255614 mRNA. Translation: AAF66069.1 . Frameshift.
RefSeqi NP_620784.2. NM_139084.2.
UniGenei Rn.228785.

3D structure databases

ProteinModelPortali Q9JK71.
SMRi Q9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 251439. 3 interactions.
IntActi Q9JK71. 2 interactions.
STRINGi 10116.ENSRNOP00000026952.

PTM databases

PhosphoSitei Q9JK71.

Proteomic databases

PaxDbi Q9JK71.
PRIDEi Q9JK71.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 245903.
KEGGi rno:245903.
UCSCi RGD:621362. rat.

Organism-specific databases

CTDi 260425.
RGDi 621362. Magi3.

Phylogenomic databases

eggNOGi COG5021.
HOGENOMi HOG000113463.
HOVERGENi HBG007091.
InParanoidi Q9JK71.
KOi K06112.
PhylomeDBi Q9JK71.

Miscellaneous databases

NextBioi 623144.
PROi Q9JK71.

Gene expression databases

Genevestigatori Q9JK71.

Family and domain databases

Gene3Di 2.30.42.10. 6 hits.
InterProi IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 4 hits.
PF00397. WW. 2 hits.
[Graphical view ]
SMARTi SM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Junctional protein MAGI-3 interacts with receptor tyrosine phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins."
    Adamsky K., Arnold K., Sabanay H., Peles E.
    J. Cell Sci. 116:1279-1289(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PTPRB.
  2. "Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
    Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
    J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTEN.
  3. "Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins."
    He J., Bellini M., Inuzuka H., Xu J., Xiong Y., Yang X., Castleberry A.M., Hall R.A.
    J. Biol. Chem. 281:2820-2827(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ADRB1.

Entry informationi

Entry nameiMAGI3_RAT
AccessioniPrimary (citable) accession number: Q9JK71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi