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Q9JK71

- MAGI3_RAT

UniProt

Q9JK71 - MAGI3_RAT

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Protein

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Gene

Magi3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1308ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Alternative name(s):
Membrane-associated guanylate kinase inverted 3
Short name:
MAGI-3
Scaffolding-like protein
Gene namesi
Name:Magi3
Synonyms:Slipr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621362. Magi3.

Subcellular locationi

Cell membrane; Peripheral membrane protein. Cell junctiontight junction. Nucleus
Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
  3. tight junction Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14701470Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3PRO_0000341409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JK71.
PRIDEiQ9JK71.

PTM databases

PhosphoSiteiQ9JK71.

Expressioni

Gene expression databases

GenevestigatoriQ9JK71.

Interactioni

Subunit structurei

Interacts with ADRB1, BAI1, LPAR2/EDG4, FZD4, FZD7, GRIN2B, TGFA and VANGL2 (By similarity). Interacts with PTEN. Interacts with ADRB1, PTPRB and unidentified tyrosine phosphorylated proteins.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Adrb1P180903EBI-696226,EBI-991303
PTENP604843EBI-696226,EBI-696162From a different organism.

Protein-protein interaction databases

BioGridi251439. 3 interactions.
IntActiQ9JK71. 2 interactions.
STRINGi10116.ENSRNOP00000026952.

Structurei

3D structure databases

ProteinModelPortaliQ9JK71.
SMRiQ9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 10891PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini116 – 290175Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST
Domaini296 – 32934WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini342 – 37534WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini413 – 49583PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini581 – 65777PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini729 – 81183PDZ 4PROSITE-ProRule annotationAdd
BLAST
Domaini852 – 93988PDZ 5PROSITE-ProRule annotationAdd
BLAST
Domaini1022 – 110483PDZ 6PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 10891Interaction with ADRB1 and TGFAAdd
BLAST
Regioni413 – 49583Interaction with PTENBy similarityAdd
BLAST
Regioni729 – 81183Interaction with BAI1By similarityAdd
BLAST
Regioni852 – 93988Interaction with LPAR2 and GRIN2BBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 94Poly-Lys
Compositional biasi240 – 2456Poly-Glu

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 6 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5021.
HOGENOMiHOG000113463.
HOVERGENiHBG007091.
InParanoidiQ9JK71.
KOiK06112.
PhylomeDBiQ9JK71.

Family and domain databases

Gene3Di2.30.42.10. 6 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 4 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JK71-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD
60 70 80 90 100
EPGGGGGTCC VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI
110 120 130 140 150
RLKTVKPGKV INKDLRHYLS LQFQKGSIDH KLQQVIRDNL YLITIPCTTR
160 170 180 190 200
APRDGEVPGV DYNFISVEQF KALEESGALL ESGTYDGNFY GTPKPPAEPS
210 220 230 240 250
PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE EEEDEDKEAV
260 270 280 290 300
NGSGSMETRE MHSESSDCWM KTVPSYNQTN RSMDFRNYMM RDENLEPLPK
310 320 330 340 350
NWEMAYTDTG TIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK
360 370 380 390 400
IEDPQYGTYY VDHLNQKTQF ENPVEEAKRK KQIGQAETHS AKTDVERAHF
410 420 430 440 450
TRDPSQLKGV LVRASLKKST MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ
460 470 480 490 500
DGKMAPGDVI VDINGNCVLG HTHADVVQMF QLVPVNQYVN LTLCRGYALP
510 520 530 540 550
DDSEDPVVDI VAATPVINGQ SLAKGEACMS TQDFKLGAMV LDQNGKSGKL
560 570 580 590 600
LSSDRLNGPS DSNEQRASLA SSGSSQPELV TIPLVKGPKG FGFAIADSPT
610 620 630 640 650
GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD
660 670 680 690 700
VPLLILRGGP CSPTKTAKMK TDTKETSGSL ETINEPTPQP MPFPPSIIRS
710 720 730 740 750
GSPKLDPSEV YLKSKTLYED KPPNTKDLDV FLRKQESGFG FRVLGGDGPD
760 770 780 790 800
QSIYIGAIIP LGAAEKDGRL RAADELMCID GIPVKGKSHK QVLDLMTTAA
810 820 830 840 850
RNGHVLLTVR RKIFYGEKQP EDESPQAFSQ SGSPRLNRTE LPTRSAPQES
860 870 880 890 900
YDVILQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGRLKV
910 920 930 940 950
GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS
960 970 980 990 1000
ARQSPALQHR PMGQAQATHI PGDRTALEGE VGKDVCSSYR HSWSDHKHLA
1010 1020 1030 1040 1050
QPDTAVISVV GSRHSQSLGC YPVELERGPR GFGFSLRGGK EYNMGLFILR
1060 1070 1080 1090 1100
LAEDGPAIKD GRIHVGDQIV EINGEPTQGI THTRAIELIQ AGGNKVLLLL
1110 1120 1130 1140 1150
RPGTGLIPDH GDWDIYSPSS SNVIYDEQPP PLPSSHSAAT FEESHVPVTE
1160 1170 1180 1190 1200
DSLIRVQTCE KAEELKDTVQ EKKSTLNGSQ PEMKYQSIQK NVSKKDPSRS
1210 1220 1230 1240 1250
HGHGDKNLLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLRSDPKGK
1260 1270 1280 1290 1300
SRDRSLSPRK GENKGQVTIK AGSGQDPCRK DRGRSSSPRK QQKIGGNSLS
1310 1320 1330 1340 1350
NTEGKLSEAG SRRAAGLSSD SPEQLPEGKE KSGVSRKDLK LSQLGKNRTR
1360 1370 1380 1390 1400
SPEKRSSKVD EASLPSKKTS DTASRVVSEK EKGRKPGTGE RSRDKTGESV
1410 1420 1430 1440 1450
QTSAKPLTQE AGEKMALSKA SEVTDRGKER AGGAPESSSP VKKAPITPGP
1460 1470
WRVPRANKVT GTAGMADKQL
Length:1,470
Mass (Da):160,563
Last modified:June 10, 2008 - v2
Checksum:i70E2AFFCE5EA8480
GO

Sequence cautioni

The sequence AAF66069.1 differs from that shown. Reason: Frameshift at several positions. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255614 mRNA. Translation: AAF66069.1. Frameshift.
RefSeqiNP_620784.2. NM_139084.2.
UniGeneiRn.228785.

Genome annotation databases

GeneIDi245903.
KEGGirno:245903.
UCSCiRGD:621362. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255614 mRNA. Translation: AAF66069.1 . Frameshift.
RefSeqi NP_620784.2. NM_139084.2.
UniGenei Rn.228785.

3D structure databases

ProteinModelPortali Q9JK71.
SMRi Q9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 251439. 3 interactions.
IntActi Q9JK71. 2 interactions.
STRINGi 10116.ENSRNOP00000026952.

PTM databases

PhosphoSitei Q9JK71.

Proteomic databases

PaxDbi Q9JK71.
PRIDEi Q9JK71.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 245903.
KEGGi rno:245903.
UCSCi RGD:621362. rat.

Organism-specific databases

CTDi 260425.
RGDi 621362. Magi3.

Phylogenomic databases

eggNOGi COG5021.
HOGENOMi HOG000113463.
HOVERGENi HBG007091.
InParanoidi Q9JK71.
KOi K06112.
PhylomeDBi Q9JK71.

Miscellaneous databases

NextBioi 623144.
PROi Q9JK71.

Gene expression databases

Genevestigatori Q9JK71.

Family and domain databases

Gene3Di 2.30.42.10. 6 hits.
InterProi IPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 4 hits.
PF00397. WW. 2 hits.
[Graphical view ]
SMARTi SM00072. GuKc. 1 hit.
SM00228. PDZ. 6 hits.
SM00456. WW. 2 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 6 hits.
SSF51045. SSF51045. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Junctional protein MAGI-3 interacts with receptor tyrosine phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins."
    Adamsky K., Arnold K., Sabanay H., Peles E.
    J. Cell Sci. 116:1279-1289(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PTPRB.
  2. "Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
    Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
    J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTEN.
  3. "Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins."
    He J., Bellini M., Inuzuka H., Xu J., Xiong Y., Yang X., Castleberry A.M., Hall R.A.
    J. Biol. Chem. 281:2820-2827(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ADRB1.

Entry informationi

Entry nameiMAGI3_RAT
AccessioniPrimary (citable) accession number: Q9JK71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3