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Q9JK71

- MAGI3_RAT

UniProt

Q9JK71 - MAGI3_RAT

Protein

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Gene

Magi3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi123 – 1308ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
    Alternative name(s):
    Membrane-associated guanylate kinase inverted 3
    Short name:
    MAGI-3
    Scaffolding-like protein
    Gene namesi
    Name:Magi3
    Synonyms:Slipr
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621362. Magi3.

    Subcellular locationi

    Cell membrane; Peripheral membrane protein. Cell junctiontight junction. Nucleus
    Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell
    3. tight junction Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Nucleus, Tight junction

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14701470Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3PRO_0000341409Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei236 – 2361PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9JK71.
    PRIDEiQ9JK71.

    PTM databases

    PhosphoSiteiQ9JK71.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9JK71.

    Interactioni

    Subunit structurei

    Interacts with ADRB1, BAI1, LPAR2/EDG4, FZD4, FZD7, GRIN2B, TGFA and VANGL2 By similarity. Interacts with PTEN. Interacts with ADRB1, PTPRB and unidentified tyrosine phosphorylated proteins.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Adrb1P180903EBI-696226,EBI-991303
    PTENP604843EBI-696226,EBI-696162From a different organism.

    Protein-protein interaction databases

    BioGridi251439. 3 interactions.
    IntActiQ9JK71. 2 interactions.
    STRINGi10116.ENSRNOP00000026952.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JK71.
    SMRiQ9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 10891PDZ 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini116 – 290175Guanylate kinase-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini296 – 32934WW 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini342 – 37534WW 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini413 – 49583PDZ 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini581 – 65777PDZ 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini729 – 81183PDZ 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini852 – 93988PDZ 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini1022 – 110483PDZ 6PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni18 – 10891Interaction with ADRB1 and TGFAAdd
    BLAST
    Regioni413 – 49583Interaction with PTENBy similarityAdd
    BLAST
    Regioni729 – 81183Interaction with BAI1By similarityAdd
    BLAST
    Regioni852 – 93988Interaction with LPAR2 and GRIN2BBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi6 – 94Poly-Lys
    Compositional biasi240 – 2456Poly-Glu

    Sequence similaritiesi

    Belongs to the MAGUK family.Curated
    Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
    Contains 6 PDZ (DHR) domains.PROSITE-ProRule annotation
    Contains 2 WW domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5021.
    HOGENOMiHOG000113463.
    HOVERGENiHBG007091.
    InParanoidiQ9JK71.
    KOiK06112.
    PhylomeDBiQ9JK71.

    Family and domain databases

    Gene3Di2.30.42.10. 6 hits.
    InterProiIPR008145. GK/Ca_channel_bsu.
    IPR008144. Guanylate_kin-like.
    IPR020590. Guanylate_kinase_CS.
    IPR027417. P-loop_NTPase.
    IPR001478. PDZ.
    IPR001202. WW_dom.
    [Graphical view]
    PfamiPF00625. Guanylate_kin. 1 hit.
    PF00595. PDZ. 4 hits.
    PF00397. WW. 2 hits.
    [Graphical view]
    SMARTiSM00072. GuKc. 1 hit.
    SM00228. PDZ. 6 hits.
    SM00456. WW. 2 hits.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 6 hits.
    SSF51045. SSF51045. 2 hits.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
    PS50052. GUANYLATE_KINASE_2. 1 hit.
    PS50106. PDZ. 6 hits.
    PS01159. WW_DOMAIN_1. 2 hits.
    PS50020. WW_DOMAIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JK71-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD     50
    EPGGGGGTCC VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI 100
    RLKTVKPGKV INKDLRHYLS LQFQKGSIDH KLQQVIRDNL YLITIPCTTR 150
    APRDGEVPGV DYNFISVEQF KALEESGALL ESGTYDGNFY GTPKPPAEPS 200
    PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE EEEDEDKEAV 250
    NGSGSMETRE MHSESSDCWM KTVPSYNQTN RSMDFRNYMM RDENLEPLPK 300
    NWEMAYTDTG TIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK 350
    IEDPQYGTYY VDHLNQKTQF ENPVEEAKRK KQIGQAETHS AKTDVERAHF 400
    TRDPSQLKGV LVRASLKKST MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ 450
    DGKMAPGDVI VDINGNCVLG HTHADVVQMF QLVPVNQYVN LTLCRGYALP 500
    DDSEDPVVDI VAATPVINGQ SLAKGEACMS TQDFKLGAMV LDQNGKSGKL 550
    LSSDRLNGPS DSNEQRASLA SSGSSQPELV TIPLVKGPKG FGFAIADSPT 600
    GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD 650
    VPLLILRGGP CSPTKTAKMK TDTKETSGSL ETINEPTPQP MPFPPSIIRS 700
    GSPKLDPSEV YLKSKTLYED KPPNTKDLDV FLRKQESGFG FRVLGGDGPD 750
    QSIYIGAIIP LGAAEKDGRL RAADELMCID GIPVKGKSHK QVLDLMTTAA 800
    RNGHVLLTVR RKIFYGEKQP EDESPQAFSQ SGSPRLNRTE LPTRSAPQES 850
    YDVILQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGRLKV 900
    GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS 950
    ARQSPALQHR PMGQAQATHI PGDRTALEGE VGKDVCSSYR HSWSDHKHLA 1000
    QPDTAVISVV GSRHSQSLGC YPVELERGPR GFGFSLRGGK EYNMGLFILR 1050
    LAEDGPAIKD GRIHVGDQIV EINGEPTQGI THTRAIELIQ AGGNKVLLLL 1100
    RPGTGLIPDH GDWDIYSPSS SNVIYDEQPP PLPSSHSAAT FEESHVPVTE 1150
    DSLIRVQTCE KAEELKDTVQ EKKSTLNGSQ PEMKYQSIQK NVSKKDPSRS 1200
    HGHGDKNLLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLRSDPKGK 1250
    SRDRSLSPRK GENKGQVTIK AGSGQDPCRK DRGRSSSPRK QQKIGGNSLS 1300
    NTEGKLSEAG SRRAAGLSSD SPEQLPEGKE KSGVSRKDLK LSQLGKNRTR 1350
    SPEKRSSKVD EASLPSKKTS DTASRVVSEK EKGRKPGTGE RSRDKTGESV 1400
    QTSAKPLTQE AGEKMALSKA SEVTDRGKER AGGAPESSSP VKKAPITPGP 1450
    WRVPRANKVT GTAGMADKQL 1470
    Length:1,470
    Mass (Da):160,563
    Last modified:June 10, 2008 - v2
    Checksum:i70E2AFFCE5EA8480
    GO

    Sequence cautioni

    The sequence AAF66069.1 differs from that shown. Reason: Frameshift at several positions.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF255614 mRNA. Translation: AAF66069.1. Frameshift.
    RefSeqiNP_620784.2. NM_139084.2.
    UniGeneiRn.228785.

    Genome annotation databases

    GeneIDi245903.
    KEGGirno:245903.
    UCSCiRGD:621362. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF255614 mRNA. Translation: AAF66069.1 . Frameshift.
    RefSeqi NP_620784.2. NM_139084.2.
    UniGenei Rn.228785.

    3D structure databases

    ProteinModelPortali Q9JK71.
    SMRi Q9JK71. Positions 292-335, 343-383, 400-506, 570-662, 726-816, 1016-1111.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251439. 3 interactions.
    IntActi Q9JK71. 2 interactions.
    STRINGi 10116.ENSRNOP00000026952.

    PTM databases

    PhosphoSitei Q9JK71.

    Proteomic databases

    PaxDbi Q9JK71.
    PRIDEi Q9JK71.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 245903.
    KEGGi rno:245903.
    UCSCi RGD:621362. rat.

    Organism-specific databases

    CTDi 260425.
    RGDi 621362. Magi3.

    Phylogenomic databases

    eggNOGi COG5021.
    HOGENOMi HOG000113463.
    HOVERGENi HBG007091.
    InParanoidi Q9JK71.
    KOi K06112.
    PhylomeDBi Q9JK71.

    Miscellaneous databases

    NextBioi 623144.
    PROi Q9JK71.

    Gene expression databases

    Genevestigatori Q9JK71.

    Family and domain databases

    Gene3Di 2.30.42.10. 6 hits.
    InterProi IPR008145. GK/Ca_channel_bsu.
    IPR008144. Guanylate_kin-like.
    IPR020590. Guanylate_kinase_CS.
    IPR027417. P-loop_NTPase.
    IPR001478. PDZ.
    IPR001202. WW_dom.
    [Graphical view ]
    Pfami PF00625. Guanylate_kin. 1 hit.
    PF00595. PDZ. 4 hits.
    PF00397. WW. 2 hits.
    [Graphical view ]
    SMARTi SM00072. GuKc. 1 hit.
    SM00228. PDZ. 6 hits.
    SM00456. WW. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 6 hits.
    SSF51045. SSF51045. 2 hits.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
    PS50052. GUANYLATE_KINASE_2. 1 hit.
    PS50106. PDZ. 6 hits.
    PS01159. WW_DOMAIN_1. 2 hits.
    PS50020. WW_DOMAIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Junctional protein MAGI-3 interacts with receptor tyrosine phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins."
      Adamsky K., Arnold K., Sabanay H., Peles E.
      J. Cell Sci. 116:1279-1289(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PTPRB.
    2. "Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
      Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
      J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTEN.
    3. "Proteomic analysis of beta1-adrenergic receptor interactions with PDZ scaffold proteins."
      He J., Bellini M., Inuzuka H., Xu J., Xiong Y., Yang X., Castleberry A.M., Hall R.A.
      J. Biol. Chem. 281:2820-2827(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ADRB1.

    Entry informationi

    Entry nameiMAGI3_RAT
    AccessioniPrimary (citable) accession number: Q9JK71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3