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Protein

Endophilin-B1

Gene

Sh3glb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells (PubMed:17086176). May recruit other proteins to membranes with high curvature. May promote membrane fusion (By similarity). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (PubMed:24523556). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (PubMed:24523556).By similarity2 Publications

Miscellaneous

Fibroblasts with a reduced level of Sh3glb1 show a defect in retrograde transport between the Golgi apparatus and the endoplasmic reticulum.

Caution

Was originally thought to have lysophosphatidic acid acyltransferase activity, but by homology with SH3GL2/endophilin A1 is unlikely to have this activity.1 Publication

GO - Molecular functioni

  • cadherin binding Source: MGI
  • fatty acid binding Source: MGI
  • identical protein binding Source: MGI
  • lysophosphatidic acid acyltransferase activity Source: MGI
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • 'de novo' posttranslational protein folding Source: MGI
  • apoptotic process Source: MGI
  • autophagic cell death Source: UniProtKB
  • cellular response to amino acid starvation Source: MGI
  • cellular response to glucose starvation Source: MGI
  • membrane fission Source: MGI
  • mitochondrion organization Source: UniProtKB
  • negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
  • phosphatidic acid biosynthetic process Source: MGI
  • phospholipid biosynthetic process Source: MGI
  • positive regulation of autophagosome assembly Source: UniProtKB
  • positive regulation of autophagy Source: UniProtKB
  • positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
  • positive regulation of membrane tubulation Source: MGI
  • positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
  • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: MGI
  • positive regulation of protein oligomerization Source: MGI
  • positive regulation of protein targeting to mitochondrion Source: MGI
  • protein localization to vacuolar membrane Source: UniProtKB
  • protein oligomerization Source: MGI
  • receptor catabolic process Source: MGI
  • regulation of cytokinesis Source: MGI
  • regulation of protein stability Source: MGI

Keywordsi

Biological processApoptosis
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-B1
Alternative name(s):
SH3 domain-containing GRB2-like protein B1
Gene namesi
Name:Sh3glb1
Synonyms:Kiaa0491
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1859730 Sh3glb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467541 – 365Endophilin-B1Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei145Phosphothreonine; by CDK5By similarity1

Post-translational modificationi

Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JK48
PaxDbiQ9JK48
PeptideAtlasiQ9JK48
PRIDEiQ9JK48

2D gel databases

REPRODUCTION-2DPAGEiQ9JK48

PTM databases

iPTMnetiQ9JK48
PhosphoSitePlusiQ9JK48
SwissPalmiQ9JK48

Expressioni

Tissue specificityi

Isoform 1 is widely expressed. Isoform 2 is brain-specific. Isoform 3 is predominantly expressed in testis, but it is also detected in liver and, at much lower levels, in skin, stomach and ovary.1 Publication

Gene expression databases

BgeeiENSMUSG00000037062
CleanExiMM_SH3GLB1
ExpressionAtlasiQ9JK48 baseline and differential
GenevisibleiQ9JK48 MM

Interactioni

Subunit structurei

Homodimer, and heterodimer with SH3GLB2 (By similarity). Binds BAX; induction of apoptosis augments BAX binding (PubMed:16227588, PubMed:17381077). Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 (PubMed:12456676, PubMed:17086176). Interacts with UVRAG; UVRAG bridges the interaction to BECN1 indicative for an association with the PI3K complex II (PI3KC3-C2) (By similarity). Isoform 3 interacts with PPP1CC; this interaction leads to the inhibition of phosphatase activity (PubMed:17381077).By similarity4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207716, 4 interactors
IntActiQ9JK48, 4 interactors
MINTiQ9JK48
STRINGi10090.ENSMUSP00000129800

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi308 – 314Combined sources7
Beta strandi331 – 335Combined sources5
Beta strandi344 – 349Combined sources6
Beta strandi352 – 357Combined sources6
Helixi358 – 360Combined sources3
Beta strandi361 – 363Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X43NMR-A298-365[»]
ProteinModelPortaliQ9JK48
SMRiQ9JK48
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JK48

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 261BARPROSITE-ProRule annotationAdd BLAST235
Domaini305 – 365SH3PROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 37Required for membrane bindingBy similarityAdd BLAST37
Regioni1 – 30Membrane-binding amphipathic helixBy similarityAdd BLAST30

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili155 – 186Sequence analysisAdd BLAST32

Domaini

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.By similarity
The SH3 domain is required and sufficient for the interaction with UVRAG.By similarity

Sequence similaritiesi

Belongs to the endophilin family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3725 Eukaryota
ENOG410XPA6 LUCA
GeneTreeiENSGT00550000074464
HOGENOMiHOG000232056
HOVERGENiHBG054448
InParanoidiQ9JK48
KOiK11248
PhylomeDBiQ9JK48
TreeFamiTF313281

Family and domain databases

CDDicd07616 BAR_Endophilin_B1, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR028503 SH3GLB1
IPR035695 SH3GLB1_BAR
PANTHERiPTHR44071:SF3 PTHR44071:SF3, 1 hit
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 2 hits
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50002 SH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JK48-1) [UniParc]FASTAAdd to basket
Also known as: Endophilin B1a, Bif-11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNIMDFNVKK LAADAGTFLS RAVQFTEEKL GQAEKTELDA HLENLLSKAE
60 70 80 90 100
CTKIWTEKIM KQTEVLLQPN PNARIEEFVY EKLDRKAPSR INNPELLGQY
110 120 130 140 150
MIDAGTEFGP GTAYGNALIK CGETQKRIGT ADRELIQTSA LNFLTPLRNF
160 170 180 190 200
IEGDYKTIAK ERKLLQNKRL DLDAAKTRLK KAKAAETKSS SEQELRITQS
210 220 230 240 250
EFDRQAEITR LLLEGISSTH AHHLRCLNDF VEAQMTYYAQ CYQYMLDLQK
260 270 280 290 300
QLGSFPSNYL SNNNQTSGTP VPYALSNAIG PSAQASTGSL VITCPSNLND
310 320 330 340 350
LKESSNNRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG
360
NQKGKVPITY LELLN
Length:365
Mass (Da):40,855
Last modified:October 1, 2000 - v1
Checksum:i1FC02795693142FB
GO
Isoform 2 (identifier: Q9JK48-2) [UniParc]FASTAAdd to basket
Also known as: Endophilin B1b

The sequence of this isoform differs from the canonical sequence as follows:
     190-190: S → SQLNSARPEGDNIMIWAEEVTK

Show »
Length:386
Mass (Da):43,239
Checksum:iEB57B8AB5DE6F627
GO
Isoform 3 (identifier: Q9JK48-3) [UniParc]FASTAAdd to basket
Also known as: Endophilin-B1t1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     356-365: Missing.

Show »
Length:355
Mass (Da):39,699
Checksum:i89E97B600228F592
GO

Sequence cautioni

The sequence BAD32237 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159A → T in BAB27575 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009277190S → SQLNSARPEGDNIMIWAEEV TK in isoform 2. 1 Publication1
Alternative sequenceiVSP_058266356 – 365Missing in isoform 3. 10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263364 mRNA Translation: AAF73438.1
AF272946 mRNA Translation: AAL59846.1
DQ205686 mRNA Translation: ABA54268.1
AK172959 mRNA Translation: BAD32237.1 Different initiation.
AK029647 mRNA Translation: BAC26545.1
AK011371 mRNA Translation: BAB27575.3
AK158415 mRNA Translation: BAE34495.1
AC134404 Genomic DNA No translation available.
BC024362 mRNA Translation: AAH24362.1
CCDSiCCDS17885.1 [Q9JK48-1]
CCDS80042.1 [Q9JK48-3]
CCDS80043.1 [Q9JK48-2]
RefSeqiNP_001268966.1, NM_001282037.1 [Q9JK48-2]
NP_001268971.1, NM_001282042.1 [Q9JK48-3]
NP_062337.1, NM_019464.3 [Q9JK48-1]
UniGeneiMm.271775
Mm.440295

Genome annotation databases

EnsembliENSMUST00000163279; ENSMUSP00000129800; ENSMUSG00000037062 [Q9JK48-1]
ENSMUST00000198254; ENSMUSP00000143312; ENSMUSG00000037062 [Q9JK48-2]
ENSMUST00000199531; ENSMUSP00000143433; ENSMUSG00000037062 [Q9JK48-3]
GeneIDi54673
KEGGimmu:54673
UCSCiuc008rpt.2 mouse [Q9JK48-1]
uc008rpw.2 mouse [Q9JK48-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSHLB1_MOUSE
AccessioniPrimary (citable) accession number: Q9JK48
Secondary accession number(s): Q3TYR7
, Q6A059, Q8CDS9, Q8VI52, Q9CT31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health