Q9JK42 (PDK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
EC=2.7.11.2

Alternative name(s):
Pyruvate dehydrogenase kinase isoform 2

Gene names

Name:

Pdk2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct pyruvate dehydrogenase (acetyl-transferring) kinase activity Inferred from electronic annotation. Source: EC two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pik3r2	O08908	3	EBI-643530,EBI-643570

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 407		[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial		PRO_0000023441
Regions
	Domain	135 – 364	230	Histidine kinase
	Nucleotide binding	251 – 258	8	ATP By similarity
	Nucleotide binding	309 – 310	2	ATP By similarity
	Nucleotide binding	325 – 330	6	ATP By similarity
Sites
	Binding site	290	1	ATP By similarity
Experimental info
	Sequence conflict	299	1	K → R in AAF72038. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9JK42 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: DBB9F3F967C6A5B1	FASTA	407	46,041
	10 20 30 40 50 60 MRWVRALLKN ASLAGAPKYI EHFSKFSPSP LSMKQFLDFG SSNACEKTSF TFLRQELPVR 70 80 90 100 110 120 LANIMKEINL LPDRVLGTPS VQLVQSWYVQ SLLDIMEFLD KDPEDHRTLS QFTDALVTIR 130 140 150 160 170 180 NRHNDVVPTM AQGVLEYKDT YGDDPVSNQN IQYFLDRFYL SRISIRMLIN QHTLIFDGST 190 200 210 220 230 240 NPAHPKHIGS IDPNCSVSDV VKDAYDMAKL LCDKYYMASP DLEIQEVNAT NANQPIHMVY 250 260 270 280 290 300 VPSHLYHMLF ELFKNAMRAT VESHESSLTL PPIKIMVALG EEDLSIKMSD RGGGVPLRKI 310 320 330 340 350 360 ERLFSYMYST APTPQPGTGG TPLAGFGYGL PISRLYAKYF QGDLQLFSME GFGTDAVIYL 370 380 390 400 KALSTDSVER LPVYNKSAWR HYQTIQEAGD WCVPSTEPKN TSTYRVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mus musculus pyruvate dehydrogenase kinase 2 cDNA." Jeoung N.H., Harris R.A. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF267660 mRNA. Translation: AAF72038.1. AL606480 Genomic DNA. Translation: CAI23968.1. CH466556 Genomic DNA. Translation: EDL15963.1. BC021764 mRNA. Translation: AAH21764.1.
IPI	IPI00122251.
RefSeq	NP_598428.2. NM_133667.2.
UniGene	Mm.29768.
3D structure databases
ProteinModelPortal	Q9JK42.
SMR	Q9JK42. Positions 12-404.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JK42. 2 interactions.
STRING	Q9JK42.
Proteomic databases
PRIDE	Q9JK42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038431; ENSMUSP00000041447; ENSMUSG00000038967.
GeneID	18604.
KEGG	mmu:18604.
Organism-specific databases
CTD	5164.
MGI	MGI:1343087. Pdk2.
Phylogenomic databases
GeneTree	ENSGT00550000074574.
HOGENOM	HBG382024.
HOVERGEN	HBG000511.
InParanoid	Q9JK42.
OrthoDB	EOG4G1MGF.
PhylomeDB	Q9JK42.
Gene expression databases
ArrayExpress	Q9JK42.
Bgee	Q9JK42.
CleanEx	MM_PDK2.
Genevestigator	Q9JK42.
GermOnline	ENSMUSG00000038967. Mus musculus.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR018955. BCDHK/PDK_N. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:1.20.140.20. BCDHK/PDK_N. 1 hit.
KO	K00898.
Pfam	PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF69012. BCDHK/PDK_N. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294514.
SOURCE	Search...

Entry information

Entry name

PDK2_MOUSE

Accession

Primary (citable) accession number: Q9JK42
Secondary accession number(s): Q8VC63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	July 27, 2011
Last modified:	November 16, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program