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Protein

Glucosamine 6-phosphate N-acetyltransferase

Gene

Gnpnat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + D-glucosamine 6-phosphate = CoA + N-acetyl-D-glucosamine 6-phosphate.By similarity

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glucosamine 6-phosphate N-acetyltransferase (Gnpnat1)
  2. Phosphoacetylglucosamine mutase (Pgm3)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61SubstrateBy similarity1
Binding sitei175SubstrateBy similarity1
Binding sitei181SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13176.
BRENDAi2.3.1.4. 3474.
ReactomeiR-MMU-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ9JK38.
UniPathwayiUPA00113; UER00529.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine 6-phosphate N-acetyltransferase (EC:2.3.1.4By similarity)
Alternative name(s):
Phosphoglucosamine acetylase
Phosphoglucosamine transacetylase
Protein EMeg32
Gene namesi
Name:Gnpnat1
Synonyms:Gna1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1858963. Gnpnat1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum-Golgi intermediate compartment Source: MGI
  • endosome membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • late endosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745541 – 184Glucosamine 6-phosphate N-acetyltransferaseAdd BLAST184

Proteomic databases

EPDiQ9JK38.
MaxQBiQ9JK38.
PaxDbiQ9JK38.
PeptideAtlasiQ9JK38.
PRIDEiQ9JK38.

PTM databases

iPTMnetiQ9JK38.
PhosphoSitePlusiQ9JK38.
SwissPalmiQ9JK38.

Expressioni

Tissue specificityi

Ubiquitous. Shows a strong differential expression pattern in adult hematopoietic precursor cells.1 Publication

Developmental stagei

Widely expressed at early stages of embryonic development but is confined to bones, skin and the hematopoietic system at later developmental stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000037722.
CleanExiMM_GNPNAT1.
GenevisibleiQ9JK38. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207615. 9 interactors.
IntActiQ9JK38. 10 interactors.
MINTiMINT-1605392.
STRINGi10090.ENSMUSP00000042860.

Structurei

3D structure databases

ProteinModelPortaliQ9JK38.
SMRiQ9JK38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 184N-acetyltransferasePROSITE-ProRule annotationAdd BLAST146

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 111Substrate bindingBy similarity4
Regioni120 – 122Substrate bindingBy similarity3
Regioni130 – 135Acetyl-CoA bindingBy similarity6
Regioni151 – 152Substrate bindingBy similarity2
Regioni165 – 167Acetyl-CoA bindingBy similarity3

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3396. Eukaryota.
COG0454. LUCA.
GeneTreeiENSGT00390000008666.
HOGENOMiHOG000106325.
HOVERGENiHBG048986.
InParanoidiQ9JK38.
KOiK00621.
OMAiGRGCYKV.
OrthoDBiEOG091G0EMF.
PhylomeDBiQ9JK38.
TreeFamiTF313790.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JK38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPDETPMFD PSLLKEVDWS QNTAIFSPAI SPTHPGEGLV LRPLCTADLN
60 70 80 90 100
KGFFKVLGQL TETGVVSPEQ FMKSFEHMKK SGDYYVTVVE DVTLGQIVAT
110 120 130 140 150
ATLIIEHKFI HSCAKRGRVE DVVVSDECRG KQLGKLLLST LTLLSKKLNC
160 170 180
YKITLECLPQ NVGFYKKFDY TVSEENYMCR RFLK
Length:184
Mass (Da):20,791
Last modified:October 1, 2000 - v1
Checksum:iC455BF12027BD8A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001006 mRNA. Translation: CAA04463.1.
AK018499 mRNA. Translation: BAB31241.1.
AK002466 mRNA. Translation: BAB22120.1.
AK007647 mRNA. Translation: BAB25161.1.
AK007722 mRNA. Translation: BAB25212.1.
AK007764 mRNA. Translation: BAB25240.1.
AK008566 mRNA. Translation: BAB25749.1.
AK011098 mRNA. Translation: BAB27395.1.
BC031116 mRNA. Translation: AAH31116.1.
CCDSiCCDS26976.1.
RefSeqiNP_062298.1. NM_019425.2.
XP_006519338.1. XM_006519275.2.
XP_006519339.1. XM_006519276.3.
UniGeneiMm.312945.

Genome annotation databases

EnsembliENSMUST00000046191; ENSMUSP00000042860; ENSMUSG00000037722.
GeneIDi54342.
KEGGimmu:54342.
UCSCiuc007tgp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001006 mRNA. Translation: CAA04463.1.
AK018499 mRNA. Translation: BAB31241.1.
AK002466 mRNA. Translation: BAB22120.1.
AK007647 mRNA. Translation: BAB25161.1.
AK007722 mRNA. Translation: BAB25212.1.
AK007764 mRNA. Translation: BAB25240.1.
AK008566 mRNA. Translation: BAB25749.1.
AK011098 mRNA. Translation: BAB27395.1.
BC031116 mRNA. Translation: AAH31116.1.
CCDSiCCDS26976.1.
RefSeqiNP_062298.1. NM_019425.2.
XP_006519338.1. XM_006519275.2.
XP_006519339.1. XM_006519276.3.
UniGeneiMm.312945.

3D structure databases

ProteinModelPortaliQ9JK38.
SMRiQ9JK38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207615. 9 interactors.
IntActiQ9JK38. 10 interactors.
MINTiMINT-1605392.
STRINGi10090.ENSMUSP00000042860.

PTM databases

iPTMnetiQ9JK38.
PhosphoSitePlusiQ9JK38.
SwissPalmiQ9JK38.

Proteomic databases

EPDiQ9JK38.
MaxQBiQ9JK38.
PaxDbiQ9JK38.
PeptideAtlasiQ9JK38.
PRIDEiQ9JK38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046191; ENSMUSP00000042860; ENSMUSG00000037722.
GeneIDi54342.
KEGGimmu:54342.
UCSCiuc007tgp.1. mouse.

Organism-specific databases

CTDi64841.
MGIiMGI:1858963. Gnpnat1.

Phylogenomic databases

eggNOGiKOG3396. Eukaryota.
COG0454. LUCA.
GeneTreeiENSGT00390000008666.
HOGENOMiHOG000106325.
HOVERGENiHBG048986.
InParanoidiQ9JK38.
KOiK00621.
OMAiGRGCYKV.
OrthoDBiEOG091G0EMF.
PhylomeDBiQ9JK38.
TreeFamiTF313790.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00529.
BioCyciMetaCyc:MONOMER-13176.
BRENDAi2.3.1.4. 3474.
ReactomeiR-MMU-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ9JK38.

Miscellaneous databases

PROiQ9JK38.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037722.
CleanExiMM_GNPNAT1.
GenevisibleiQ9JK38. MM.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGNA1_MOUSE
AccessioniPrimary (citable) accession number: Q9JK38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.