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Protein

Origin recognition complex subunit 3

Gene

Orc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: MGI
  • DNA replication initiation Source: GO_Central
  • glial cell proliferation Source: GOC-OWL
  • neural precursor cell proliferation Source: MGI
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68616. Assembly of the ORC complex at the origin of replication.
R-MMU-68689. CDC6 association with the ORC:origin complex.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69298. Association of licensing factors with the pre-replicative complex.
R-MMU-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 3
Alternative name(s):
Origin recognition complex subunit Latheo
Gene namesi
Name:Orc3
Synonyms:Orc3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1354944. Orc3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270841 – 715Origin recognition complex subunit 3Add BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei523PhosphoserineBy similarity1
Isoform 2 (identifier: Q9JK30-2)
Modified residuei522PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JK30.
PaxDbiQ9JK30.
PeptideAtlasiQ9JK30.
PRIDEiQ9JK30.

PTM databases

iPTMnetiQ9JK30.
PhosphoSitePlusiQ9JK30.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040044.
ExpressionAtlasiQ9JK30. baseline and differential.
GenevisibleiQ9JK30. MM.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity).By similarity

Protein-protein interaction databases

BioGridi206124. 1 interactor.
STRINGi10090.ENSMUSP00000048319.

Structurei

3D structure databases

ProteinModelPortaliQ9JK30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC3 family.Curated

Phylogenomic databases

eggNOGiKOG2538. Eukaryota.
ENOG410XT1W. LUCA.
GeneTreeiENSGT00390000011376.
HOGENOMiHOG000047785.
HOVERGENiHBG007599.
InParanoidiQ9JK30.
KOiK02605.
OMAiHLECGRM.
OrthoDBiEOG091G0AI5.
PhylomeDBiQ9JK30.
TreeFamiTF101093.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR020795. ORC3.
[Graphical view]
PANTHERiPTHR12748. PTHR12748. 1 hit.
PfamiPF07034. ORC3_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JK30-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTGPRTMAT SSVSKGCFVF KPDFKKRKVF VPIEDYFNNE ELDSEDSKLR
60 70 80 90 100
FETYSLLWQR MKSETEQLQE ELNENLFDNL VDFLQKSHPE FQKNSRDWGS
110 120 130 140 150
QMKFREIPTA ALILGVNVTD HDVILRSLTE TLQNNVTPYV VSLQAKDCPD
160 170 180 190 200
VKHFLQKFTS QLMDCCVDRH SKEVTSGKAL KKTNYSMDSL CSWYSAVTQK
210 220 230 240 250
ADHKVTIKKR TASGHWRSPP VVLILKSMES FTSKVLQDFI TISSQHLHEF
260 270 280 290 300
PLILIFGIAT SPVIIHRLLP HSVSSLLCVE LFQSLSCEQH LTVVLDKLLL
310 320 330 340 350
TPQFPFKLSK KALQVLTNIF LYHDFSIQSF IKGIKLSLLE HFYSQPLSVL
360 370 380 390 400
CCDLSEAKKR VNVFSVSQCE NIRRLPSFRR YVENQPLGKQ VALLTNETFL
410 420 430 440 450
KEKTQSLLED LHVYHINYFL VLRCLHNFTS SLPKYPLGRQ IRELYCTCLE
460 470 480 490 500
KKIWDSEEYK SALQLLRMLA KDELVSILQR CIEVLDSSTE KQLGNTTQKI
510 520 530 540 550
KDFLTQFQNL DADSKEEEDA CGSQPKGLQK TDLYHLQKSL LEMKELRRTK
560 570 580 590 600
KPTKFEMLRE NVMNFIDNLV RDYLLPPESQ PLHEVVYFSA ANTLREHLNA
610 620 630 640 650
APRIALHTAL NNPYYYLKNE ELEGCIPNTA PDICIAYKLH LECSLLINLV
660 670 680 690 700
DWAEAFATVV TAAEKMDANS TVSEEMSEVI HARFIRAVSE LELLGFIKPT
710
KQKTDHVARL TWGGC
Length:715
Mass (Da):82,342
Last modified:October 1, 2000 - v1
Checksum:i1E915A719A2E33EB
GO
Isoform 2 (identifier: Q9JK30-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     512-512: Missing.

Show »
Length:714
Mass (Da):82,271
Checksum:i13E9EDC7B01D1C7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114L → F in BAC39695 (PubMed:16141072).Curated1
Sequence conflicti212A → P in BAD91665 (PubMed:15634681).Curated1
Sequence conflicti212A → P in AAH61252 (PubMed:15489334).Curated1
Sequence conflicti360R → G in BAC32339 (PubMed:16141072).Curated1
Sequence conflicti645L → R in BAD91665 (PubMed:15634681).Curated1
Sequence conflicti645L → R in AAH61252 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026407512Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132830 mRNA. Translation: CAB76399.1.
AB190257 mRNA. Translation: BAD91665.2.
AK045388 mRNA. Translation: BAC32339.1.
AK086579 mRNA. Translation: BAC39695.1.
AL807397, AL928738 Genomic DNA. Translation: CAM16474.1.
AL807397, AL928738 Genomic DNA. Translation: CAM16475.1.
AL928738, AL807397 Genomic DNA. Translation: CAM26869.1.
AL928738, AL807397 Genomic DNA. Translation: CAM26870.1.
BC061252 mRNA. Translation: AAH61252.1.
CCDSiCCDS18028.1. [Q9JK30-1]
CCDS51137.1. [Q9JK30-2]
RefSeqiNP_001153035.1. NM_001159563.1. [Q9JK30-2]
NP_056639.3. NM_015824.4. [Q9JK30-1]
UniGeneiMm.399624.

Genome annotation databases

EnsembliENSMUST00000048706; ENSMUSP00000048319; ENSMUSG00000040044. [Q9JK30-1]
ENSMUST00000108142; ENSMUSP00000103777; ENSMUSG00000040044. [Q9JK30-2]
GeneIDi50793.
KEGGimmu:50793.
UCSCiuc008sgb.2. mouse. [Q9JK30-1]
uc008sgd.1. mouse. [Q9JK30-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132830 mRNA. Translation: CAB76399.1.
AB190257 mRNA. Translation: BAD91665.2.
AK045388 mRNA. Translation: BAC32339.1.
AK086579 mRNA. Translation: BAC39695.1.
AL807397, AL928738 Genomic DNA. Translation: CAM16474.1.
AL807397, AL928738 Genomic DNA. Translation: CAM16475.1.
AL928738, AL807397 Genomic DNA. Translation: CAM26869.1.
AL928738, AL807397 Genomic DNA. Translation: CAM26870.1.
BC061252 mRNA. Translation: AAH61252.1.
CCDSiCCDS18028.1. [Q9JK30-1]
CCDS51137.1. [Q9JK30-2]
RefSeqiNP_001153035.1. NM_001159563.1. [Q9JK30-2]
NP_056639.3. NM_015824.4. [Q9JK30-1]
UniGeneiMm.399624.

3D structure databases

ProteinModelPortaliQ9JK30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206124. 1 interactor.
STRINGi10090.ENSMUSP00000048319.

PTM databases

iPTMnetiQ9JK30.
PhosphoSitePlusiQ9JK30.

Proteomic databases

EPDiQ9JK30.
PaxDbiQ9JK30.
PeptideAtlasiQ9JK30.
PRIDEiQ9JK30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048706; ENSMUSP00000048319; ENSMUSG00000040044. [Q9JK30-1]
ENSMUST00000108142; ENSMUSP00000103777; ENSMUSG00000040044. [Q9JK30-2]
GeneIDi50793.
KEGGimmu:50793.
UCSCiuc008sgb.2. mouse. [Q9JK30-1]
uc008sgd.1. mouse. [Q9JK30-2]

Organism-specific databases

CTDi23595.
MGIiMGI:1354944. Orc3.

Phylogenomic databases

eggNOGiKOG2538. Eukaryota.
ENOG410XT1W. LUCA.
GeneTreeiENSGT00390000011376.
HOGENOMiHOG000047785.
HOVERGENiHBG007599.
InParanoidiQ9JK30.
KOiK02605.
OMAiHLECGRM.
OrthoDBiEOG091G0AI5.
PhylomeDBiQ9JK30.
TreeFamiTF101093.

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68616. Assembly of the ORC complex at the origin of replication.
R-MMU-68689. CDC6 association with the ORC:origin complex.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69298. Association of licensing factors with the pre-replicative complex.
R-MMU-69300. Removal of licensing factors from origins.

Miscellaneous databases

ChiTaRSiOrc3. mouse.
PROiQ9JK30.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040044.
ExpressionAtlasiQ9JK30. baseline and differential.
GenevisibleiQ9JK30. MM.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR020795. ORC3.
[Graphical view]
PANTHERiPTHR12748. PTHR12748. 1 hit.
PfamiPF07034. ORC3_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiORC3_MOUSE
AccessioniPrimary (citable) accession number: Q9JK30
Secondary accession number(s): A2AMF4
, A2AMF5, Q6P8H2, Q8BR83, Q8C395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.