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Protein

Origin recognition complex subunit 3

Gene

Orc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity).By similarity

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: MGI
  • DNA replication initiation Source: GO_Central
  • neural precursor cell proliferation Source: MGI
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-68616 Assembly of the ORC complex at the origin of replication
R-MMU-68689 CDC6 association with the ORC:origin complex
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68867 Assembly of the pre-replicative complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 3
Alternative name(s):
Origin recognition complex subunit Latheo
Gene namesi
Name:Orc3
Synonyms:Orc3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1354944 Orc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270841 – 715Origin recognition complex subunit 3Add BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei523PhosphoserineBy similarity1
Isoform 2 (identifier: Q9JK30-2)
Modified residuei522PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JK30
PaxDbiQ9JK30
PeptideAtlasiQ9JK30
PRIDEiQ9JK30

PTM databases

iPTMnetiQ9JK30
PhosphoSitePlusiQ9JK30

Expressioni

Gene expression databases

BgeeiENSMUSG00000040044
ExpressionAtlasiQ9JK30 baseline and differential
GenevisibleiQ9JK30 MM

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity).By similarity

Protein-protein interaction databases

BioGridi206124, 1 interactor
CORUMiQ9JK30
STRINGi10090.ENSMUSP00000048319

Structurei

3D structure databases

ProteinModelPortaliQ9JK30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC3 family.Curated

Phylogenomic databases

eggNOGiKOG2538 Eukaryota
ENOG410XT1W LUCA
GeneTreeiENSGT00390000011376
HOGENOMiHOG000047785
HOVERGENiHBG007599
InParanoidiQ9JK30
KOiK02605
OMAiECGRMIN
OrthoDBiEOG091G0AI5
PhylomeDBiQ9JK30
TreeFamiTF101093

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR020795 ORC3
PANTHERiPTHR12748 PTHR12748, 1 hit
PfamiView protein in Pfam
PF07034 ORC3_N, 1 hit
SUPFAMiSSF48371 SSF48371, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JK30-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTGPRTMAT SSVSKGCFVF KPDFKKRKVF VPIEDYFNNE ELDSEDSKLR
60 70 80 90 100
FETYSLLWQR MKSETEQLQE ELNENLFDNL VDFLQKSHPE FQKNSRDWGS
110 120 130 140 150
QMKFREIPTA ALILGVNVTD HDVILRSLTE TLQNNVTPYV VSLQAKDCPD
160 170 180 190 200
VKHFLQKFTS QLMDCCVDRH SKEVTSGKAL KKTNYSMDSL CSWYSAVTQK
210 220 230 240 250
ADHKVTIKKR TASGHWRSPP VVLILKSMES FTSKVLQDFI TISSQHLHEF
260 270 280 290 300
PLILIFGIAT SPVIIHRLLP HSVSSLLCVE LFQSLSCEQH LTVVLDKLLL
310 320 330 340 350
TPQFPFKLSK KALQVLTNIF LYHDFSIQSF IKGIKLSLLE HFYSQPLSVL
360 370 380 390 400
CCDLSEAKKR VNVFSVSQCE NIRRLPSFRR YVENQPLGKQ VALLTNETFL
410 420 430 440 450
KEKTQSLLED LHVYHINYFL VLRCLHNFTS SLPKYPLGRQ IRELYCTCLE
460 470 480 490 500
KKIWDSEEYK SALQLLRMLA KDELVSILQR CIEVLDSSTE KQLGNTTQKI
510 520 530 540 550
KDFLTQFQNL DADSKEEEDA CGSQPKGLQK TDLYHLQKSL LEMKELRRTK
560 570 580 590 600
KPTKFEMLRE NVMNFIDNLV RDYLLPPESQ PLHEVVYFSA ANTLREHLNA
610 620 630 640 650
APRIALHTAL NNPYYYLKNE ELEGCIPNTA PDICIAYKLH LECSLLINLV
660 670 680 690 700
DWAEAFATVV TAAEKMDANS TVSEEMSEVI HARFIRAVSE LELLGFIKPT
710
KQKTDHVARL TWGGC
Length:715
Mass (Da):82,342
Last modified:October 1, 2000 - v1
Checksum:i1E915A719A2E33EB
GO
Isoform 2 (identifier: Q9JK30-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     512-512: Missing.

Show »
Length:714
Mass (Da):82,271
Checksum:i13E9EDC7B01D1C7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114L → F in BAC39695 (PubMed:16141072).Curated1
Sequence conflicti212A → P in BAD91665 (PubMed:15634681).Curated1
Sequence conflicti212A → P in AAH61252 (PubMed:15489334).Curated1
Sequence conflicti360R → G in BAC32339 (PubMed:16141072).Curated1
Sequence conflicti645L → R in BAD91665 (PubMed:15634681).Curated1
Sequence conflicti645L → R in AAH61252 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026407512Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132830 mRNA Translation: CAB76399.1
AB190257 mRNA Translation: BAD91665.2
AK045388 mRNA Translation: BAC32339.1
AK086579 mRNA Translation: BAC39695.1
AL807397, AL928738 Genomic DNA Translation: CAM16474.1
AL807397, AL928738 Genomic DNA Translation: CAM16475.1
AL928738, AL807397 Genomic DNA Translation: CAM26869.1
AL928738, AL807397 Genomic DNA Translation: CAM26870.1
BC061252 mRNA Translation: AAH61252.1
CCDSiCCDS18028.1 [Q9JK30-1]
CCDS51137.1 [Q9JK30-2]
RefSeqiNP_001153035.1, NM_001159563.1 [Q9JK30-2]
NP_056639.3, NM_015824.4 [Q9JK30-1]
UniGeneiMm.399624

Genome annotation databases

EnsembliENSMUST00000048706; ENSMUSP00000048319; ENSMUSG00000040044 [Q9JK30-1]
ENSMUST00000108142; ENSMUSP00000103777; ENSMUSG00000040044 [Q9JK30-2]
GeneIDi50793
KEGGimmu:50793
UCSCiuc008sgb.2 mouse [Q9JK30-1]
uc008sgd.1 mouse [Q9JK30-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiORC3_MOUSE
AccessioniPrimary (citable) accession number: Q9JK30
Secondary accession number(s): A2AMF4
, A2AMF5, Q6P8H2, Q8BR83, Q8C395
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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