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Q9JK30 (ORC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Origin recognition complex subunit 3
Alternative name(s):
Origin recognition complex subunit Latheo
Gene names
Name:Orc3
Synonyms:Orc3l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication By similarity. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 By similarity.

Subunit structure

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the ORC3 family.

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Traceable author statement Ref.1. Source: MGI

neural precursor cell proliferation

Inferred from mutant phenotype PubMed 21185282PubMed 23349620. Source: MGI

   Cellular_componentnuclear origin of replication recognition complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JK30-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JK30-2)

The sequence of this isoform differs from the canonical sequence as follows:
     512-512: Missing.
Note: Contains a phosphoserine at position 522.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 715715Origin recognition complex subunit 3
PRO_0000127084

Amino acid modifications

Modified residue5231Phosphoserine By similarity

Natural variations

Alternative sequence5121Missing in isoform 2.
VSP_026407

Experimental info

Sequence conflict1141L → F in BAC39695. Ref.3
Sequence conflict2121A → P in BAD91665. Ref.2
Sequence conflict2121A → P in AAH61252. Ref.5
Sequence conflict3601R → G in BAC32339. Ref.3
Sequence conflict6451L → R in BAD91665. Ref.2
Sequence conflict6451L → R in AAH61252. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 1E915A719A2E33EB

FASTA71582,342
        10         20         30         40         50         60 
MHTGPRTMAT SSVSKGCFVF KPDFKKRKVF VPIEDYFNNE ELDSEDSKLR FETYSLLWQR 

        70         80         90        100        110        120 
MKSETEQLQE ELNENLFDNL VDFLQKSHPE FQKNSRDWGS QMKFREIPTA ALILGVNVTD 

       130        140        150        160        170        180 
HDVILRSLTE TLQNNVTPYV VSLQAKDCPD VKHFLQKFTS QLMDCCVDRH SKEVTSGKAL 

       190        200        210        220        230        240 
KKTNYSMDSL CSWYSAVTQK ADHKVTIKKR TASGHWRSPP VVLILKSMES FTSKVLQDFI 

       250        260        270        280        290        300 
TISSQHLHEF PLILIFGIAT SPVIIHRLLP HSVSSLLCVE LFQSLSCEQH LTVVLDKLLL 

       310        320        330        340        350        360 
TPQFPFKLSK KALQVLTNIF LYHDFSIQSF IKGIKLSLLE HFYSQPLSVL CCDLSEAKKR 

       370        380        390        400        410        420 
VNVFSVSQCE NIRRLPSFRR YVENQPLGKQ VALLTNETFL KEKTQSLLED LHVYHINYFL 

       430        440        450        460        470        480 
VLRCLHNFTS SLPKYPLGRQ IRELYCTCLE KKIWDSEEYK SALQLLRMLA KDELVSILQR 

       490        500        510        520        530        540 
CIEVLDSSTE KQLGNTTQKI KDFLTQFQNL DADSKEEEDA CGSQPKGLQK TDLYHLQKSL 

       550        560        570        580        590        600 
LEMKELRRTK KPTKFEMLRE NVMNFIDNLV RDYLLPPESQ PLHEVVYFSA ANTLREHLNA 

       610        620        630        640        650        660 
APRIALHTAL NNPYYYLKNE ELEGCIPNTA PDICIAYKLH LECSLLINLV DWAEAFATVV 

       670        680        690        700        710 
TAAEKMDANS TVSEEMSEVI HARFIRAVSE LELLGFIKPT KQKTDHVARL TWGGC 

« Hide

Isoform 2 [UniParc].

Checksum: 13E9EDC7B01D1C7E
Show »

FASTA71482,271

References

« Hide 'large scale' references
[1]"Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex."
Springer J., Nanda I., Hoehn K., Schmid M., Grummt F.
Cytogenet. Cell Genet. 87:245-251(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Novel splicing variant of mouse Orc1 is deficient in nuclear translocation and resistant for proteasome-mediated degradation."
Miyake Y., Mizuno T., Yanagi K., Hanaoka F.
J. Biol. Chem. 280:12643-12652(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Corpora quadrigemina and Head.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Pituitary.
[6]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-522 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ132830 mRNA. Translation: CAB76399.1.
AB190257 mRNA. Translation: BAD91665.2.
AK045388 mRNA. Translation: BAC32339.1.
AK086579 mRNA. Translation: BAC39695.1.
AL807397, AL928738 Genomic DNA. Translation: CAM16474.1.
AL807397, AL928738 Genomic DNA. Translation: CAM16475.1.
AL928738, AL807397 Genomic DNA. Translation: CAM26869.1.
AL928738, AL807397 Genomic DNA. Translation: CAM26870.1.
BC061252 mRNA. Translation: AAH61252.1.
CCDSCCDS18028.1. [Q9JK30-1]
CCDS51137.1. [Q9JK30-2]
RefSeqNP_001153035.1. NM_001159563.1. [Q9JK30-2]
NP_056639.3. NM_015824.4. [Q9JK30-1]
UniGeneMm.399624.

3D structure databases

ProteinModelPortalQ9JK30.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid206124. 1 interaction.

PTM databases

PhosphoSiteQ9JK30.

Proteomic databases

PaxDbQ9JK30.
PRIDEQ9JK30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048706; ENSMUSP00000048319; ENSMUSG00000040044. [Q9JK30-1]
ENSMUST00000108142; ENSMUSP00000103777; ENSMUSG00000040044. [Q9JK30-2]
GeneID50793.
KEGGmmu:50793.
UCSCuc008sgb.2. mouse. [Q9JK30-1]
uc008sgd.1. mouse. [Q9JK30-2]

Organism-specific databases

CTD23595.
MGIMGI:1354944. Orc3.

Phylogenomic databases

eggNOGNOG296366.
GeneTreeENSGT00390000011376.
HOGENOMHOG000047785.
HOVERGENHBG007599.
InParanoidQ9JK30.
KOK02605.
OMACFVFKPN.
OrthoDBEOG78PV8B.
PhylomeDBQ9JK30.
TreeFamTF101093.

Gene expression databases

ArrayExpressQ9JK30.
BgeeQ9JK30.
GenevestigatorQ9JK30.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR020795. ORC3.
[Graphical view]
PANTHERPTHR12748. PTHR12748. 1 hit.
PfamPF07034. ORC3_N. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 4 hits.
ProtoNetSearch...

Other

ChiTaRSORC3. mouse.
NextBio307789.
PROQ9JK30.
SOURCESearch...

Entry information

Entry nameORC3_MOUSE
AccessionPrimary (citable) accession number: Q9JK30
Secondary accession number(s): A2AMF4 expand/collapse secondary AC list , A2AMF5, Q6P8H2, Q8BR83, Q8C395
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot