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Protein

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma

Gene

Ppp2r3c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate MCM3AP phosphorylation through phosphatase recruitment. May act as a negative regulator of ABCB1 expression and function through the dephosphorylation of ABCB1 by TFPI2/PPP2R3C complex. May play a role in the activation-induced cell death of B-cells.By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi286 – 297121Sequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • activation of protein kinase activity Source: MGI
  • B cell homeostasis Source: MGI
  • cortical cytoskeleton organization Source: GO_Central
  • microtubule cytoskeleton organization Source: GO_Central
  • positive regulation of B cell differentiation Source: MGI
  • regulation of antimicrobial humoral response Source: MGI
  • regulation of B cell activation Source: MGI
  • regulation of mitochondrial depolarization Source: MGI
  • spleen development Source: MGI
  • T cell homeostasis Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma
Alternative name(s):
Protein phosphatase subunit G5PR1 Publication
Gene namesi
Name:Ppp2r3c
Synonyms:G5pr1 Publication
ORF Names:MNCb-1932
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1930009. Ppp2r3c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display a reduction in the number of mature B-cells and an impaired B-cell proliferation upon B-Cell receptor cross-linking probably due to a loss of inhibition of BCR-induced apoptosis. PPP2R3C overexpression protects B-cells from activation-induced cell death.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gammaPRO_0000277834Add
BLAST

Proteomic databases

PRIDEiQ9JK24.

PTM databases

PhosphoSiteiQ9JK24.

Expressioni

Tissue specificityi

Expressed in all tissues tested including heart, brain, spleen, thymus, lung, liver, kidney and testis.1 Publication

Developmental stagei

Detected from 6 to 12 dpc in whole embryos and from 14 to 18 dpc in the heads of the embryos. Expressed in central nervous system, spine, face, pharynx, limbs and viscera at 11 dpc.1 Publication

Inductioni

Up-regulated upon B-cell receptor cross-linking.1 Publication

Gene expression databases

BgeeiQ9JK24.
GenevisibleiQ9JK24. MM.

Interactioni

Subunit structurei

Interacts with MCM3AP/GANP, PPP5C, and the phosphatase 2A core enzyme composed of the PPP2CA catalytic subunit and the constant regulatory subunit PPP2R1A. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021410.

Structurei

3D structure databases

ProteinModelPortaliQ9JK24.
SMRiQ9JK24. Positions 188-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini273 – 30836EF-hand 1Add
BLAST
Domaini341 – 37636EF-hand 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi226 – 2294Poly-Phe

Sequence similaritiesi

Contains 2 EF-hand domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG279493.
GeneTreeiENSGT00530000063265.
HOGENOMiHOG000005699.
HOVERGENiHBG055242.
InParanoidiQ9JK24.
KOiK11583.
OMAiQESNWFS.
OrthoDBiEOG7M6D73.
PhylomeDBiQ9JK24.
TreeFamiTF318412.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JK24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWKDVLRRR LASPNTDPKR KKSEQELKDE EMDLFTKYYS EWKGGRKNTN
60 70 80 90 100
EFYKTIPRFY YRLPAEDEVL LQKLREESRA VFLQRKSREL LDNEELQNLW
110 120 130 140 150
FLLDKHQIPP MIGEEAMINY ENFLKVGEKA GPKCKQFFTA KVFAKLLHTD
160 170 180 190 200
SYGRISIMQF FNYVMRKVWL HQTRIGLSLY DVAGQGYLRE SDLENYILEL
210 220 230 240 250
IPTLPQLDGL EKSFYSFYVC TAVRKFFFFL DPLRTGKIKI QDILACSFLD
260 270 280 290 300
DLLELRDEEL SKESQETNWF SAPSALRVYG QYLNLDKDHN GMLSKEELSR
310 320 330 340 350
YGTATMTNVF LDRVFQECLT YDGEMDYKTY LDFVLALENR KEPAALQYIF
360 370 380 390 400
KLLDIENKGY LNVFSLNYFF RAIQELMKIH GQDPVSFQDV KDEIFDMVKP
410 420 430 440 450
KDPLKISLQD LINSNQGDTV TTILIDLNGF WTYENREALV ANDNENSADL

DDT
Length:453
Mass (Da):53,407
Last modified:March 1, 2001 - v2
Checksum:i8FD29927385EAD81
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti335 – 3351L → P in BAA95061 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238247 mRNA. Translation: CAB88038.2.
AB041577 mRNA. Translation: BAA95061.1.
AK013403 mRNA. Translation: BAB28835.1.
AK028254 mRNA. Translation: BAC25845.1.
AK040589 mRNA. Translation: BAC30637.1.
AK087572 mRNA. Translation: BAC39933.1.
AK088240 mRNA. Translation: BAC40229.1.
BC024754 mRNA. Translation: AAH24754.1.
BC138295 mRNA. Translation: AAI38296.1.
BC138296 mRNA. Translation: AAI38297.1.
CCDSiCCDS36450.1.
RefSeqiNP_067504.2. NM_021529.3.
UniGeneiMm.427307.

Genome annotation databases

EnsembliENSMUST00000021410; ENSMUSP00000021410; ENSMUSG00000021022.
GeneIDi59032.
KEGGimmu:59032.
UCSCiuc007nom.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238247 mRNA. Translation: CAB88038.2.
AB041577 mRNA. Translation: BAA95061.1.
AK013403 mRNA. Translation: BAB28835.1.
AK028254 mRNA. Translation: BAC25845.1.
AK040589 mRNA. Translation: BAC30637.1.
AK087572 mRNA. Translation: BAC39933.1.
AK088240 mRNA. Translation: BAC40229.1.
BC024754 mRNA. Translation: AAH24754.1.
BC138295 mRNA. Translation: AAI38296.1.
BC138296 mRNA. Translation: AAI38297.1.
CCDSiCCDS36450.1.
RefSeqiNP_067504.2. NM_021529.3.
UniGeneiMm.427307.

3D structure databases

ProteinModelPortaliQ9JK24.
SMRiQ9JK24. Positions 188-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021410.

PTM databases

PhosphoSiteiQ9JK24.

Proteomic databases

PRIDEiQ9JK24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021410; ENSMUSP00000021410; ENSMUSG00000021022.
GeneIDi59032.
KEGGimmu:59032.
UCSCiuc007nom.1. mouse.

Organism-specific databases

CTDi55012.
MGIiMGI:1930009. Ppp2r3c.

Phylogenomic databases

eggNOGiNOG279493.
GeneTreeiENSGT00530000063265.
HOGENOMiHOG000005699.
HOVERGENiHBG055242.
InParanoidiQ9JK24.
KOiK11583.
OMAiQESNWFS.
OrthoDBiEOG7M6D73.
PhylomeDBiQ9JK24.
TreeFamiTF318412.

Miscellaneous databases

NextBioi314626.
PROiQ9JK24.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JK24.
GenevisibleiQ9JK24. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR."
    Kono Y., Maeda K., Kuwahara K., Yamamoto H., Miyamoto E., Yonezawa K., Takagi K., Sakaguchi N.
    Genes Cells 7:821-834(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MCM3AP; PPP2CA; PPP2R1A AND PPP5C, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: B-cell.
  2. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo, Head, Oviduct and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Brain and Mammary tumor.
  5. "Defining a holoprosencephaly locus on human chromosome 14q13 and characterization of potential candidate genes."
    Kamnasaran D., Chen C.-P., Devriendt K., Mehta L., Cox D.W.
    Genomics 85:608-621(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
    Tissue: Brain.
  6. "Protein phosphatase subunit G5PR is needed for inhibition of B cell receptor-induced apoptosis."
    Xing Y., Igarashi H., Wang X., Sakaguchi N.
    J. Exp. Med. 202:707-719(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "BCR-crosslinking induces a transcription of protein phosphatase component G5PR that is required for mature B-cell survival."
    Huq Ronny F.M., Igarashi H., Sakaguchi N.
    Biochem. Biophys. Res. Commun. 340:338-346(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiP2R3C_MOUSE
AccessioniPrimary (citable) accession number: Q9JK24
Secondary accession number(s): B2RR97, Q9CSA6, Q9JJD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.