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Protein

Arf-GAP with dual PH domain-containing protein 2

Gene

Adap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Binding of phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 3,4-bisphosphate occurs at a much lower affinity. Possesses a stoichiometry of two binding sites for InsP4 with identical affinity (By similarity).By similarityCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: RGD
  • GTPase activator activity Source: UniProtKB-KW
  • inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  • phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with dual PH domain-containing protein 2
Alternative name(s):
Centaurin-alpha-2
Short name:
Cnt-a2
Gene namesi
Name:Adap2
Synonyms:Centa2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708487. Adap2.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Constitutively associated with the plasma membrane. Excluded from the nucleus (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • mitochondrial envelope Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi151 – 1511R → C: Almost complete loss of InsP4 binding. 1 Publication
Mutagenesisi275 – 2751R → C: No loss of InsP4 binding. Almost complete loss of InsP4 binding; when associated with C-151. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Arf-GAP with dual PH domain-containing protein 2PRO_0000074208Add
BLAST

Proteomic databases

PaxDbiQ9JK15.
PRIDEiQ9JK15.

PTM databases

PhosphoSiteiQ9JK15.

Expressioni

Tissue specificityi

Expressed in many tissues, with highest levels in fat, heart and skeletal muscle. Also detected in kidney, liver and lung.1 Publication

Interactioni

Protein-protein interaction databases

MINTiMINT-4998321.
STRINGi10116.ENSRNOP00000053112.

Structurei

3D structure databases

ProteinModelPortaliQ9JK15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 130122Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini131 – 232102PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini254 – 360107PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0703. Eukaryota.
COG5347. LUCA.
HOGENOMiHOG000006719.
HOVERGENiHBG050888.
InParanoidiQ9JK15.
PhylomeDBiQ9JK15.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JK15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRERNKKR LLELLQAAGT GNGHCADCGA ADPDWASYKL GVFICLHCSG
60 70 80 90 100
VHRNFPDISK VKSVRLDFWD DSMVEFMTHN GNLSVKAKFE ARVPTFYYVP
110 120 130 140 150
QASDCLVLKE QWIRAKYERQ EFMAEKAVSP PGDREGFLWK RGRDNSQFLR
160 170 180 190 200
RRFVLLSREG LLKYYTKEEG KTPKAIINIK DLNATFQTEK IGHPHGLQIT
210 220 230 240 250
YRKEGQTRNL FVYHDSGKEI VDWFNALRAA RLQYLKLAFP DLPESELVPL
260 270 280 290 300
ITRNYLKQGF MEKTGPKHRE PFKKRWFALD PQERRLLYYK NPLDAFEQGQ
310 320 330 340 350
VFLGSNEQGY EVWEGLPQGI RGNRWKVGLT VITPERKFVF TCPTEKEQRE
360 370
WLESLRGVLS SPLSPLHLLT TSAKSG
Length:376
Mass (Da):43,524
Last modified:October 1, 2000 - v1
Checksum:iE357392F38F07166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238993 mRNA. Translation: CAB88403.1.
RefSeqiNP_064486.1. NM_020101.1.
UniGeneiRn.203877.

Genome annotation databases

GeneIDi56826.
KEGGirno:56826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238993 mRNA. Translation: CAB88403.1.
RefSeqiNP_064486.1. NM_020101.1.
UniGeneiRn.203877.

3D structure databases

ProteinModelPortaliQ9JK15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4998321.
STRINGi10116.ENSRNOP00000053112.

PTM databases

PhosphoSiteiQ9JK15.

Proteomic databases

PaxDbiQ9JK15.
PRIDEiQ9JK15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56826.
KEGGirno:56826.

Organism-specific databases

CTDi55803.
RGDi708487. Adap2.

Phylogenomic databases

eggNOGiKOG0703. Eukaryota.
COG5347. LUCA.
HOGENOMiHOG000006719.
HOVERGENiHBG050888.
InParanoidiQ9JK15.
PhylomeDBiQ9JK15.

Miscellaneous databases

PROiQ9JK15.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle."
    Whitley P., Gibbard A.M., Koumanov F., Oldfield S., Kilgour E.E., Prestwich G.D., Holman G.D.
    Eur. J. Cell Biol. 81:222-230(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PTDINS(4,5)P2; PTDINS(3,4,5)P3 AND INS(1,3,4,5)P4, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-151 AND ARG-275.
    Tissue: Adipocyte.

Entry informationi

Entry nameiADAP2_RAT
AccessioniPrimary (citable) accession number: Q9JK15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.