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Protein

Cyclic nucleotide-gated cation channel beta-3

Gene

Cngb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cGMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones (By similarity). Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGA3.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841cGMPBy similarity
Binding sitei596 – 5961cGMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi524 – 668145cGMPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated cation channel beta-3
Alternative name(s):
Cone photoreceptor cGMP-gated channel subunit beta
Cyclic nucleotide-gated cation channel modulatory subunit
Cyclic nucleotide-gated channel beta-3
Short name:
CNG channel beta-3
Cyclic nucleotide-gated channel subunit CNG6
Gene namesi
Name:Cngb3
Synonyms:Cng6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1353562. Cngb3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 209209CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei210 – 23021Helical; Name=H1Sequence AnalysisAdd
BLAST
Topological domaini231 – 24212ExtracellularSequence AnalysisAdd
BLAST
Transmembranei243 – 26321Helical; Name=H2Sequence AnalysisAdd
BLAST
Topological domaini264 – 29431CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei295 – 31521Helical; Name=H3Sequence AnalysisAdd
BLAST
Topological domaini316 – 35136ExtracellularSequence AnalysisAdd
BLAST
Transmembranei352 – 37221Helical; Name=H4Sequence AnalysisAdd
BLAST
Topological domaini373 – 40937CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei410 – 43021Helical; Name=H5Sequence AnalysisAdd
BLAST
Topological domaini431 – 568138ExtracellularSequence AnalysisAdd
BLAST
Transmembranei569 – 58921Helical; Name=H6Sequence AnalysisAdd
BLAST
Topological domaini590 – 694105CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • photoreceptor outer segment Source: MGI
  • plasma membrane Source: MGI
  • transmembrane transporter complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694Cyclic nucleotide-gated cation channel beta-3PRO_0000219321Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JJZ9.
PRIDEiQ9JJZ9.

PTM databases

PhosphoSiteiQ9JJZ9.

Expressioni

Tissue specificityi

Small subset of retinal photorecptor cells and testis.1 Publication

Gene expression databases

BgeeiQ9JJZ9.
CleanExiMM_CNGB3.
GenevisibleiQ9JJZ9. MM.

Interactioni

Subunit structurei

Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100064.

Structurei

3D structure databases

ProteinModelPortaliQ9JJZ9.
SMRiQ9JJZ9. Positions 442-628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG289446.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000231425.
HOVERGENiHBG051038.
InParanoidiQ9JJZ9.
KOiK04953.
OMAiKMPLTEY.
OrthoDBiEOG744T8M.
PhylomeDBiQ9JJZ9.
TreeFamiTF318250.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKSLTVKFN KVNPMEGRME KKLCPNLSSL SQPTIAQGDN QSEKEPLRSR
60 70 80 90 100
TPITFEKSHS KEDNSTGENS LRDFTPNPDP ECRAELTRTM AEMEKTRTGK
110 120 130 140 150
ERPVSFKTKV LETSIINEYT DAHLHNLVER MRERTALYKK TLTEEENFPE
160 170 180 190 200
VEASSQTAMS TNISPKQENN SKLKEHQDTF SFKPQRVPVK EHLRRMILPR
210 220 230 240 250
SIDSYTDRVY LLWLLLVTIA YNWNCWLLPV RLVFPCQTPD NKNYWIITDI
260 270 280 290 300
VCDIIYLCDI LLIQPRLQFV RGGEIIVDSN ELKRNYRSST KFRMDVASLL
310 320 330 340 350
PFEVLYIFFG VNPIFRANRI LKYTSFFEFN HHLESIMDKA YVYRVIRTTG
360 370 380 390 400
YLLFLLHINA CVYYWASDYE GIGSTKWVYN GEGNKYLRCF YWAVRTLITI
410 420 430 440 450
GGLPEPQTSF EIVFQFLNFF SGVFVFSSLI GQMRDVIGAA TANQNYFQAC
460 470 480 490 500
MDHIIAYMNK YSIPQSVQYR VRTWLEYTWN SQRILDESNL LENLPTAMQL
510 520 530 540 550
SIALDINFSI IDKVELFKGC DTQMIYDLLL RLKSTIYLPG DFVCKKGEIG
560 570 580 590 600
KEMYIIKHGE VQVLGGPDGA QVLVTLKAGS VFGEISLLAK GGGNRRTADV
610 620 630 640 650
VAHGFANLLT LDKKTLQEIL LHYPTSKKLL MKKAKILLSQ KGKTTQAIPA
660 670 680 690
RPGPAFLFPP KEETPRMLKV LLGNTGKVDL GRLLKGKRKT TTQK
Length:694
Mass (Da):79,722
Last modified:October 1, 2000 - v1
Checksum:i0B9F9CF3B180DA82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243572 mRNA. Translation: CAB71152.1.
CCDSiCCDS17990.1.
RefSeqiNP_038955.1. NM_013927.2.
UniGeneiMm.445778.

Genome annotation databases

EnsembliENSMUST00000102999; ENSMUSP00000100064; ENSMUSG00000056494.
GeneIDi30952.
KEGGimmu:30952.
UCSCiuc008sbx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243572 mRNA. Translation: CAB71152.1.
CCDSiCCDS17990.1.
RefSeqiNP_038955.1. NM_013927.2.
UniGeneiMm.445778.

3D structure databases

ProteinModelPortaliQ9JJZ9.
SMRiQ9JJZ9. Positions 442-628.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100064.

PTM databases

PhosphoSiteiQ9JJZ9.

Proteomic databases

PaxDbiQ9JJZ9.
PRIDEiQ9JJZ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102999; ENSMUSP00000100064; ENSMUSG00000056494.
GeneIDi30952.
KEGGimmu:30952.
UCSCiuc008sbx.1. mouse.

Organism-specific databases

CTDi54714.
MGIiMGI:1353562. Cngb3.

Phylogenomic databases

eggNOGiNOG289446.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000231425.
HOVERGENiHBG051038.
InParanoidiQ9JJZ9.
KOiK04953.
OMAiKMPLTEY.
OrthoDBiEOG744T8M.
PhylomeDBiQ9JJZ9.
TreeFamiTF318250.

Miscellaneous databases

NextBioi307424.
PROiQ9JJZ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJZ9.
CleanExiMM_CNGB3.
GenevisibleiQ9JJZ9. MM.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and functional characterization of a new modulatory cyclic nucleotide-gated channel subunit from mouse retina."
    Gerstner A., Zong X., Hofmann F., Biel M.
    J. Neurosci. 20:1324-1332(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiCNGB3_MOUSE
AccessioniPrimary (citable) accession number: Q9JJZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.