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Q9JJZ8 (CNGA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclic nucleotide-gated cation channel alpha-3
Alternative name(s):
Cone photoreceptor cGMP-gated channel subunit alpha
Cyclic nucleotide-gated channel alpha-3
Short name=CNG channel alpha-3
Short name=CNG-3
Short name=CNG3
Gene names
Name:Cnga3
Synonyms:Cng3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones By similarity. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3. Ref.4

Subunit structure

Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits By similarity. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Prominently expressed in retina.

Domain

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits By similarity.

Sequence similarities

Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Ontologies

Keywords
   Biological processIon transport
Sensory transduction
Transport
Vision
   Cellular componentMembrane
   DomainCoiled coil
Transmembrane
Transmembrane helix
   LigandcGMP
cGMP-binding
Nucleotide-binding
   Molecular functionIon channel
Ligand-gated ion channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphototransduction, visible light

Inferred from Biological aspect of Ancestor. Source: RefGenome

potassium ion transmembrane transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of membrane potential

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to corticosteroid

Inferred from electronic annotation. Source: Ensembl

response to magnesium ion

Inferred from electronic annotation. Source: Ensembl

retinal cone cell development

Inferred from mutant phenotype PubMed 21052544. Source: MGI

visual perception

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

perikaryon

Inferred from electronic annotation. Source: Ensembl

photoreceptor outer segment

Inferred from direct assay Ref.1PubMed 15634774. Source: MGI

photoreceptor outer segment membrane

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay Ref.1. Source: MGI

   Molecular_functioncGMP binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

intracellular cAMP activated cation channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

intracellular cGMP activated cation channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

intracellular cyclic nucleotide activated cation channel activity

Inferred from physical interaction Ref.4. Source: MGI

voltage-gated potassium channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 631631Cyclic nucleotide-gated cation channel alpha-3
PRO_0000219318

Regions

Transmembrane112 – 13322Helical; Potential
Transmembrane244 – 26421Helical; Potential
Transmembrane320 – 34021Helical; Potential
Nucleotide binding423 – 546124cGMP
Coiled coil567 – 61044 By similarity

Sites

Binding site4901cGMP Potential
Binding site5051cGMP Potential

Experimental info

Sequence conflict1101Y → C in CAB89685. Ref.1
Sequence conflict1571L → V in CAB42891. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9JJZ8 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4FAD66E3A8A3FE6C

FASTA63172,702
        10         20         30         40         50         60 
MAKVNTQCSQ PSPTQLSIKN ADRDLDHVEN GLGRVSRLII SIRAWASRHL HDEDQTPDSF 

        70         80         90        100        110        120 
LDRFHGSELK EVSTRESNAQ PNPGEQKPPD GGEGRKEEPI VVDPSSNIYY RWLTAIALPV 

       130        140        150        160        170        180 
FYNWCLLVCR ACFDELQSEH LTLWLVLDYS ADVLYVLDML VRARTGFLEQ GLMVRDTKRL 

       190        200        210        220        230        240 
WKHYTKTLHF KLDILSLIPT DLAYLKLGVN YPELRFNRLL KFSRLFEFFD RTETRTNYPN 

       250        260        270        280        290        300 
VFRIGNLVLY TLIIIHWNAC IYFAISKFIG FGTDSWVYPN TSKPEYARLS RKYIYSLYWS 

       310        320        330        340        350        360 
TLTLTTIGET PPPVKDEEYL FVVIDFLVGI LIFATIVGNV GSMISNMNAP RVEFQAKIDS 

       370        380        390        400        410        420 
VKQYMQFRKV TKDLETRVIR WFDYLWANRK TVDEKEVLKN LPDKLKAEIA INVHLDTLKK 

       430        440        450        460        470        480 
VRIFQDCEAG LLVELVLKLR PTVFSPGDYI CKKGDIGREM YIIKEGKLAV VADDGVTQFV 

       490        500        510        520        530        540 
VLSDGSYFGE ISILNIKGSK SGNRRTANIR SIGYSDLFCL SKDDLMEALT EYPDAKRALE 

       550        560        570        580        590        600 
EKGRQILMKD NLIDEDLVAA RVDTRDVEEK VEYLESSLDI LQTRFARLLA EYSASQMKLK 

       610        620        630 
QRLTRLESQM NRRCCGFSPD RENSEDASKT D 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel alpha-subunit to all cone photoreceptors in the mouse retina."
Hirano A.A., Hack I., Waessle H., Duvoisin R.M.
J. Comp. Neurol. 421:80-94(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Retina.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[3]"Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3."
Biel M., Seeliger M., Pfeifer A., Kohler K., Gerstner A., Ludwig A., Jaissle G., Fauser S., Zrenner E., Hofmann F.
Proc. Natl. Acad. Sci. U.S.A. 96:7553-7557(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-631.
Strain: 129/Sv.
[4]"Molecular cloning and functional characterization of a new modulatory cyclic nucleotide-gated channel subunit from mouse retina."
Gerstner A., Zong X., Hofmann F., Biel M.
J. Neurosci. 20:1324-1332(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243933 mRNA. Translation: CAB89685.1.
BC035272 mRNA. Translation: AAH35272.1.
BC049145 mRNA. Translation: AAH49145.1.
AJ238239, AJ238240, AJ238241 Genomic DNA. Translation: CAB42891.1.
RefSeqNP_001268939.1. NM_001282010.1.
NP_034048.1. NM_009918.2.
XP_006495702.1. XM_006495639.1.
XP_006495703.1. XM_006495640.1.
UniGeneMm.214224.

3D structure databases

ProteinModelPortalQ9JJZ8.
SMRQ9JJZ8. Positions 246-341, 348-540, 567-610.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000027288.

PTM databases

PhosphoSiteQ9JJZ8.

Proteomic databases

PRIDEQ9JJZ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027288; ENSMUSP00000027288; ENSMUSG00000026114.
GeneID12790.
KEGGmmu:12790.
UCSCuc007ari.1. mouse.

Organism-specific databases

CTD1261.
MGIMGI:1341818. Cnga3.

Phylogenomic databases

eggNOGNOG300025.
GeneTreeENSGT00550000074376.
HOGENOMHOG000007898.
HOVERGENHBG000281.
InParanoidQ8CFV6.
KOK04950.
OMAMAKINTQ.
OrthoDBEOG771268.
TreeFamTF319048.

Gene expression databases

BgeeQ9JJZ8.
CleanExMM_CNGA3.
GenevestigatorQ9JJZ8.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio282194.
PROQ9JJZ8.
SOURCESearch...

Entry information

Entry nameCNGA3_MOUSE
AccessionPrimary (citable) accession number: Q9JJZ8
Secondary accession number(s): Q8CFV6, Q9WV01
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot