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Protein

Cyclic nucleotide-gated cation channel alpha-3

Gene

Cnga3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones (By similarity). Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei490 – 4901cGMPSequence Analysis
Binding sitei505 – 5051cGMPSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi423 – 546124cGMPAdd
BLAST

GO - Molecular functioni

  1. cGMP binding Source: MGI
  2. intracellular cAMP activated cation channel activity Source: GO_Central
  3. intracellular cGMP activated cation channel activity Source: MGI
  4. intracellular cyclic nucleotide activated cation channel activity Source: MGI
  5. voltage-gated potassium channel activity Source: GO_Central

GO - Biological processi

  1. cation transport Source: MGI
  2. phototransduction, visible light Source: GO_Central
  3. potassium ion transmembrane transport Source: GO_Central
  4. regulation of membrane potential Source: GO_Central
  5. response to cAMP Source: Ensembl
  6. response to corticosteroid Source: Ensembl
  7. response to magnesium ion Source: Ensembl
  8. retinal cone cell development Source: MGI
  9. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated cation channel alpha-3
Alternative name(s):
Cone photoreceptor cGMP-gated channel subunit alpha
Cyclic nucleotide-gated channel alpha-3
Short name:
CNG channel alpha-3
Short name:
CNG-3
Short name:
CNG3
Gene namesi
Name:Cnga3
Synonyms:Cng3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1341818. Cnga3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei112 – 13322HelicalSequence AnalysisAdd
BLAST
Transmembranei244 – 26421HelicalSequence AnalysisAdd
BLAST
Transmembranei320 – 34021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. dendrite Source: Ensembl
  3. integral component of plasma membrane Source: GO_Central
  4. perikaryon Source: Ensembl
  5. photoreceptor outer segment Source: MGI
  6. photoreceptor outer segment membrane Source: Ensembl
  7. plasma membrane Source: MGI
  8. transmembrane transporter complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 631631Cyclic nucleotide-gated cation channel alpha-3PRO_0000219318Add
BLAST

Proteomic databases

PRIDEiQ9JJZ8.

PTM databases

PhosphoSiteiQ9JJZ8.

Expressioni

Tissue specificityi

Prominently expressed in retina.

Gene expression databases

BgeeiQ9JJZ8.
CleanExiMM_CNGA3.
GenevestigatoriQ9JJZ8.

Interactioni

Subunit structurei

Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027288.

Structurei

3D structure databases

ProteinModelPortaliQ9JJZ8.
SMRiQ9JJZ8. Positions 345-540, 567-610.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili567 – 61044By similarityAdd
BLAST

Domaini

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300025.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ9JJZ8.
KOiK04950.
OMAiMAKINTQ.
OrthoDBiEOG771268.
TreeFamiTF319048.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVNTQCSQ PSPTQLSIKN ADRDLDHVEN GLGRVSRLII SIRAWASRHL
60 70 80 90 100
HDEDQTPDSF LDRFHGSELK EVSTRESNAQ PNPGEQKPPD GGEGRKEEPI
110 120 130 140 150
VVDPSSNIYY RWLTAIALPV FYNWCLLVCR ACFDELQSEH LTLWLVLDYS
160 170 180 190 200
ADVLYVLDML VRARTGFLEQ GLMVRDTKRL WKHYTKTLHF KLDILSLIPT
210 220 230 240 250
DLAYLKLGVN YPELRFNRLL KFSRLFEFFD RTETRTNYPN VFRIGNLVLY
260 270 280 290 300
TLIIIHWNAC IYFAISKFIG FGTDSWVYPN TSKPEYARLS RKYIYSLYWS
310 320 330 340 350
TLTLTTIGET PPPVKDEEYL FVVIDFLVGI LIFATIVGNV GSMISNMNAP
360 370 380 390 400
RVEFQAKIDS VKQYMQFRKV TKDLETRVIR WFDYLWANRK TVDEKEVLKN
410 420 430 440 450
LPDKLKAEIA INVHLDTLKK VRIFQDCEAG LLVELVLKLR PTVFSPGDYI
460 470 480 490 500
CKKGDIGREM YIIKEGKLAV VADDGVTQFV VLSDGSYFGE ISILNIKGSK
510 520 530 540 550
SGNRRTANIR SIGYSDLFCL SKDDLMEALT EYPDAKRALE EKGRQILMKD
560 570 580 590 600
NLIDEDLVAA RVDTRDVEEK VEYLESSLDI LQTRFARLLA EYSASQMKLK
610 620 630
QRLTRLESQM NRRCCGFSPD RENSEDASKT D
Length:631
Mass (Da):72,702
Last modified:July 27, 2011 - v2
Checksum:i4FAD66E3A8A3FE6C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101Y → C in CAB89685 (PubMed:10813773).Curated
Sequence conflicti157 – 1571L → V in CAB42891 (PubMed:10377453).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243933 mRNA. Translation: CAB89685.1.
BC035272 mRNA. Translation: AAH35272.1.
BC049145 mRNA. Translation: AAH49145.1.
AJ238239, AJ238240, AJ238241 Genomic DNA. Translation: CAB42891.1.
CCDSiCCDS14890.1.
RefSeqiNP_001268939.1. NM_001282010.1.
NP_034048.1. NM_009918.2.
UniGeneiMm.214224.

Genome annotation databases

EnsembliENSMUST00000027288; ENSMUSP00000027288; ENSMUSG00000026114.
ENSMUST00000194195; ENSMUSP00000142075; ENSMUSG00000026114.
GeneIDi12790.
KEGGimmu:12790.
UCSCiuc007ari.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243933 mRNA. Translation: CAB89685.1.
BC035272 mRNA. Translation: AAH35272.1.
BC049145 mRNA. Translation: AAH49145.1.
AJ238239, AJ238240, AJ238241 Genomic DNA. Translation: CAB42891.1.
CCDSiCCDS14890.1.
RefSeqiNP_001268939.1. NM_001282010.1.
NP_034048.1. NM_009918.2.
UniGeneiMm.214224.

3D structure databases

ProteinModelPortaliQ9JJZ8.
SMRiQ9JJZ8. Positions 345-540, 567-610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027288.

PTM databases

PhosphoSiteiQ9JJZ8.

Proteomic databases

PRIDEiQ9JJZ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027288; ENSMUSP00000027288; ENSMUSG00000026114.
ENSMUST00000194195; ENSMUSP00000142075; ENSMUSG00000026114.
GeneIDi12790.
KEGGimmu:12790.
UCSCiuc007ari.1. mouse.

Organism-specific databases

CTDi1261.
MGIiMGI:1341818. Cnga3.

Phylogenomic databases

eggNOGiNOG300025.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ9JJZ8.
KOiK04950.
OMAiMAKINTQ.
OrthoDBiEOG771268.
TreeFamiTF319048.

Miscellaneous databases

NextBioi282194.
PROiQ9JJZ8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJZ8.
CleanExiMM_CNGA3.
GenevestigatoriQ9JJZ8.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel alpha-subunit to all cone photoreceptors in the mouse retina."
    Hirano A.A., Hack I., Waessle H., Duvoisin R.M.
    J. Comp. Neurol. 421:80-94(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3."
    Biel M., Seeliger M., Pfeifer A., Kohler K., Gerstner A., Ludwig A., Jaissle G., Fauser S., Zrenner E., Hofmann F.
    Proc. Natl. Acad. Sci. U.S.A. 96:7553-7557(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-631.
    Strain: 129/Sv.
  4. "Molecular cloning and functional characterization of a new modulatory cyclic nucleotide-gated channel subunit from mouse retina."
    Gerstner A., Zong X., Hofmann F., Biel M.
    J. Neurosci. 20:1324-1332(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.

Entry informationi

Entry nameiCNGA3_MOUSE
AccessioniPrimary (citable) accession number: Q9JJZ8
Secondary accession number(s): Q8CFV6, Q9WV01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.