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Q9JJZ8

- CNGA3_MOUSE

UniProt

Q9JJZ8 - CNGA3_MOUSE

Protein

Cyclic nucleotide-gated cation channel alpha-3

Gene

Cnga3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones By similarity. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei490 – 4901cGMPSequence Analysis
    Binding sitei505 – 5051cGMPSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi423 – 546124cGMPAdd
    BLAST

    GO - Molecular functioni

    1. cGMP binding Source: RefGenome
    2. intracellular cAMP activated cation channel activity Source: RefGenome
    3. intracellular cGMP activated cation channel activity Source: RefGenome
    4. intracellular cyclic nucleotide activated cation channel activity Source: MGI
    5. protein binding Source: MGI
    6. voltage-gated potassium channel activity Source: RefGenome

    GO - Biological processi

    1. phototransduction, visible light Source: RefGenome
    2. potassium ion transmembrane transport Source: RefGenome
    3. regulation of membrane potential Source: RefGenome
    4. response to cAMP Source: Ensembl
    5. response to corticosteroid Source: Ensembl
    6. response to magnesium ion Source: Ensembl
    7. retinal cone cell development Source: MGI
    8. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel

    Keywords - Biological processi

    Ion transport, Sensory transduction, Transport, Vision

    Keywords - Ligandi

    cGMP, cGMP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclic nucleotide-gated cation channel alpha-3
    Alternative name(s):
    Cone photoreceptor cGMP-gated channel subunit alpha
    Cyclic nucleotide-gated channel alpha-3
    Short name:
    CNG channel alpha-3
    Short name:
    CNG-3
    Short name:
    CNG3
    Gene namesi
    Name:Cnga3
    Synonyms:Cng3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1341818. Cnga3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. dendrite Source: Ensembl
    3. integral component of plasma membrane Source: RefGenome
    4. perikaryon Source: Ensembl
    5. photoreceptor outer segment Source: MGI
    6. photoreceptor outer segment membrane Source: Ensembl
    7. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 631631Cyclic nucleotide-gated cation channel alpha-3PRO_0000219318Add
    BLAST

    Proteomic databases

    PRIDEiQ9JJZ8.

    PTM databases

    PhosphoSiteiQ9JJZ8.

    Expressioni

    Tissue specificityi

    Prominently expressed in retina.

    Gene expression databases

    BgeeiQ9JJZ8.
    CleanExiMM_CNGA3.
    GenevestigatoriQ9JJZ8.

    Interactioni

    Subunit structurei

    Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000027288.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JJZ8.
    SMRiQ9JJZ8. Positions 292-540, 567-610.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei112 – 13322HelicalSequence AnalysisAdd
    BLAST
    Transmembranei244 – 26421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei320 – 34021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili567 – 61044By similarityAdd
    BLAST

    Domaini

    The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300025.
    GeneTreeiENSGT00550000074376.
    HOGENOMiHOG000007898.
    HOVERGENiHBG000281.
    InParanoidiQ8CFV6.
    KOiK04950.
    OMAiMAKINTQ.
    OrthoDBiEOG771268.
    TreeFamiTF319048.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    SMARTiSM00100. cNMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JJZ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKVNTQCSQ PSPTQLSIKN ADRDLDHVEN GLGRVSRLII SIRAWASRHL    50
    HDEDQTPDSF LDRFHGSELK EVSTRESNAQ PNPGEQKPPD GGEGRKEEPI 100
    VVDPSSNIYY RWLTAIALPV FYNWCLLVCR ACFDELQSEH LTLWLVLDYS 150
    ADVLYVLDML VRARTGFLEQ GLMVRDTKRL WKHYTKTLHF KLDILSLIPT 200
    DLAYLKLGVN YPELRFNRLL KFSRLFEFFD RTETRTNYPN VFRIGNLVLY 250
    TLIIIHWNAC IYFAISKFIG FGTDSWVYPN TSKPEYARLS RKYIYSLYWS 300
    TLTLTTIGET PPPVKDEEYL FVVIDFLVGI LIFATIVGNV GSMISNMNAP 350
    RVEFQAKIDS VKQYMQFRKV TKDLETRVIR WFDYLWANRK TVDEKEVLKN 400
    LPDKLKAEIA INVHLDTLKK VRIFQDCEAG LLVELVLKLR PTVFSPGDYI 450
    CKKGDIGREM YIIKEGKLAV VADDGVTQFV VLSDGSYFGE ISILNIKGSK 500
    SGNRRTANIR SIGYSDLFCL SKDDLMEALT EYPDAKRALE EKGRQILMKD 550
    NLIDEDLVAA RVDTRDVEEK VEYLESSLDI LQTRFARLLA EYSASQMKLK 600
    QRLTRLESQM NRRCCGFSPD RENSEDASKT D 631
    Length:631
    Mass (Da):72,702
    Last modified:July 27, 2011 - v2
    Checksum:i4FAD66E3A8A3FE6C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101Y → C in CAB89685. (PubMed:10813773)Curated
    Sequence conflicti157 – 1571L → V in CAB42891. (PubMed:10377453)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243933 mRNA. Translation: CAB89685.1.
    BC035272 mRNA. Translation: AAH35272.1.
    BC049145 mRNA. Translation: AAH49145.1.
    AJ238239, AJ238240, AJ238241 Genomic DNA. Translation: CAB42891.1.
    CCDSiCCDS14890.1.
    RefSeqiNP_001268939.1. NM_001282010.1.
    NP_034048.1. NM_009918.2.
    XP_006495702.1. XM_006495639.1.
    XP_006495703.1. XM_006495640.1.
    UniGeneiMm.214224.

    Genome annotation databases

    EnsembliENSMUST00000027288; ENSMUSP00000027288; ENSMUSG00000026114.
    GeneIDi12790.
    KEGGimmu:12790.
    UCSCiuc007ari.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243933 mRNA. Translation: CAB89685.1 .
    BC035272 mRNA. Translation: AAH35272.1 .
    BC049145 mRNA. Translation: AAH49145.1 .
    AJ238239 , AJ238240 , AJ238241 Genomic DNA. Translation: CAB42891.1 .
    CCDSi CCDS14890.1.
    RefSeqi NP_001268939.1. NM_001282010.1.
    NP_034048.1. NM_009918.2.
    XP_006495702.1. XM_006495639.1.
    XP_006495703.1. XM_006495640.1.
    UniGenei Mm.214224.

    3D structure databases

    ProteinModelPortali Q9JJZ8.
    SMRi Q9JJZ8. Positions 292-540, 567-610.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000027288.

    PTM databases

    PhosphoSitei Q9JJZ8.

    Proteomic databases

    PRIDEi Q9JJZ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027288 ; ENSMUSP00000027288 ; ENSMUSG00000026114 .
    GeneIDi 12790.
    KEGGi mmu:12790.
    UCSCi uc007ari.1. mouse.

    Organism-specific databases

    CTDi 1261.
    MGIi MGI:1341818. Cnga3.

    Phylogenomic databases

    eggNOGi NOG300025.
    GeneTreei ENSGT00550000074376.
    HOGENOMi HOG000007898.
    HOVERGENi HBG000281.
    InParanoidi Q8CFV6.
    KOi K04950.
    OMAi MAKINTQ.
    OrthoDBi EOG771268.
    TreeFami TF319048.

    Miscellaneous databases

    NextBioi 282194.
    PROi Q9JJZ8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JJZ8.
    CleanExi MM_CNGA3.
    Genevestigatori Q9JJZ8.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    SMARTi SM00100. cNMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel alpha-subunit to all cone photoreceptors in the mouse retina."
      Hirano A.A., Hack I., Waessle H., Duvoisin R.M.
      J. Comp. Neurol. 421:80-94(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    3. "Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3."
      Biel M., Seeliger M., Pfeifer A., Kohler K., Gerstner A., Ludwig A., Jaissle G., Fauser S., Zrenner E., Hofmann F.
      Proc. Natl. Acad. Sci. U.S.A. 96:7553-7557(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-631.
      Strain: 129/Sv.
    4. "Molecular cloning and functional characterization of a new modulatory cyclic nucleotide-gated channel subunit from mouse retina."
      Gerstner A., Zong X., Hofmann F., Biel M.
      J. Neurosci. 20:1324-1332(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiCNGA3_MOUSE
    AccessioniPrimary (citable) accession number: Q9JJZ8
    Secondary accession number(s): Q8CFV6, Q9WV01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3