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Protein

Ubiquitin-conjugating enzyme E2 J1

Gene

Ube2j1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).PROSITE-ProRule annotation

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of retrograde protein transport, ER to cytosol Source: MGI
  • protein N-linked glycosylation via asparagine Source: UniProtKB
  • regulation of tumor necrosis factor biosynthetic process Source: MGI
  • spermatid development Source: MGI
  • ubiquitin-dependent ERAD pathway Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 J1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme J1
Non-canonical ubiquitin-conjugating enzyme 1
Short name:
NCUBE-1
Gene namesi
Name:Ube2j1
Synonyms:Ncube1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1926245 Ube2j1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 282CytoplasmicSequence analysisAdd BLAST282
Transmembranei283 – 303Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini304 – 318LumenalSequence analysisAdd BLAST15

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825951 – 318Ubiquitin-conjugating enzyme E2 J1Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184PhosphoserineBy similarity1
Modified residuei266PhosphoserineCombined sources1
Modified residuei268PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JJZ4
MaxQBiQ9JJZ4
PaxDbiQ9JJZ4
PeptideAtlasiQ9JJZ4
PRIDEiQ9JJZ4

PTM databases

iPTMnetiQ9JJZ4
PhosphoSitePlusiQ9JJZ4

Expressioni

Gene expression databases

BgeeiENSMUSG00000028277
CleanExiMM_UBE2J1
ExpressionAtlasiQ9JJZ4 baseline and differential
GenevisibleiQ9JJZ4 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207860, 1 interactor
STRINGi10090.ENSMUSP00000118333

Structurei

3D structure databases

ProteinModelPortaliQ9JJZ4
SMRiQ9JJZ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0428 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00530000063379
HOGENOMiHOG000167871
HOVERGENiHBG058959
InParanoidiQ9JJZ4
KOiK10578
OMAiDFCCEMC
OrthoDBiEOG091G0JU3
PhylomeDBiQ9JJZ4
TreeFamiTF101124

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JJZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD
60 70 80 90 100
SDFDGGVYHG RIVLPPEYPM KPPSIILLTA NGRFEVGKKI CLSISGHHPE
110 120 130 140 150
TWQPSWSIRT ALLAIIGFMP TKGEGAIGSL DYTPEERRAL AKKSQDFCCE
160 170 180 190 200
GCGSAMKDVL LPLKSGSGSS QADQEAKELA RQISFKAEVN SSGKTIAESD
210 220 230 240 250
LNQCFSLNDS QDDLPTTFQG ATASTSYGAQ NPSGAPLPQP TQPAPKNTSM
260 270 280 290 300
SPRQRRAQQQ SQRRPSTSPD VLQGQPPRAH HTEHGGSAML IIILTLALAA
310
LIFRRIYLAN EYIFDFEL
Length:318
Mass (Da):34,990
Last modified:September 13, 2004 - v2
Checksum:i61A409ADF397EA36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286G → V in CAB83217 (PubMed:10708578).Curated1
Sequence conflicti289M → V in CAB83217 (PubMed:10708578).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245899 mRNA Translation: CAB83217.1
AK003050 mRNA Translation: BAB22532.1
AK031669 mRNA Translation: BAC27502.1
BC024623 mRNA Translation: AAH24623.1
CCDSiCCDS18019.1
RefSeqiNP_062532.2, NM_019586.3
UniGeneiMm.259095

Genome annotation databases

EnsembliENSMUST00000124992; ENSMUSP00000118333; ENSMUSG00000028277
GeneIDi56228
KEGGimmu:56228
UCSCiuc008sfp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiUB2J1_MOUSE
AccessioniPrimary (citable) accession number: Q9JJZ4
Secondary accession number(s): Q9DC92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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