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Protein

Ubiquitin-conjugating enzyme E2 J1

Gene

Ube2j1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 J1 (EC:6.3.2.19)
Alternative name(s):
Non-canonical ubiquitin-conjugating enzyme 1
Short name:
NCUBE-1
Gene namesi
Name:Ube2j1
Synonyms:Ncube1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1926245. Ube2j1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 282282CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei283 – 30321Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini304 – 31815LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Ubiquitin-conjugating enzyme E2 J1PRO_0000082595Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei266 – 2661Phosphoserine1 Publication
Modified residuei268 – 2681PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JJZ4.
PaxDbiQ9JJZ4.
PRIDEiQ9JJZ4.

PTM databases

PhosphoSiteiQ9JJZ4.

Expressioni

Gene expression databases

BgeeiQ9JJZ4.
CleanExiMM_UBE2J1.
ExpressionAtlasiQ9JJZ4. baseline and differential.
GenevisibleiQ9JJZ4. MM.

Interactioni

Protein-protein interaction databases

BioGridi207860. 1 interaction.
STRINGi10090.ENSMUSP00000118333.

Structurei

3D structure databases

ProteinModelPortaliQ9JJZ4.
SMRiQ9JJZ4. Positions 9-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00530000063379.
HOGENOMiHOG000167871.
HOVERGENiHBG058959.
InParanoidiQ9JJZ4.
KOiK10578.
OMAiDFCCEMC.
OrthoDBiEOG7HB59N.
PhylomeDBiQ9JJZ4.
TreeFamiTF101124.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD
60 70 80 90 100
SDFDGGVYHG RIVLPPEYPM KPPSIILLTA NGRFEVGKKI CLSISGHHPE
110 120 130 140 150
TWQPSWSIRT ALLAIIGFMP TKGEGAIGSL DYTPEERRAL AKKSQDFCCE
160 170 180 190 200
GCGSAMKDVL LPLKSGSGSS QADQEAKELA RQISFKAEVN SSGKTIAESD
210 220 230 240 250
LNQCFSLNDS QDDLPTTFQG ATASTSYGAQ NPSGAPLPQP TQPAPKNTSM
260 270 280 290 300
SPRQRRAQQQ SQRRPSTSPD VLQGQPPRAH HTEHGGSAML IIILTLALAA
310
LIFRRIYLAN EYIFDFEL
Length:318
Mass (Da):34,990
Last modified:September 13, 2004 - v2
Checksum:i61A409ADF397EA36
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti286 – 2861G → V in CAB83217 (PubMed:10708578).Curated
Sequence conflicti289 – 2891M → V in CAB83217 (PubMed:10708578).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245899 mRNA. Translation: CAB83217.1.
AK003050 mRNA. Translation: BAB22532.1.
AK031669 mRNA. Translation: BAC27502.1.
BC024623 mRNA. Translation: AAH24623.1.
CCDSiCCDS18019.1.
RefSeqiNP_062532.2. NM_019586.3.
UniGeneiMm.259095.

Genome annotation databases

EnsembliENSMUST00000124992; ENSMUSP00000118333; ENSMUSG00000028277.
GeneIDi56228.
KEGGimmu:56228.
UCSCiuc008sfp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245899 mRNA. Translation: CAB83217.1.
AK003050 mRNA. Translation: BAB22532.1.
AK031669 mRNA. Translation: BAC27502.1.
BC024623 mRNA. Translation: AAH24623.1.
CCDSiCCDS18019.1.
RefSeqiNP_062532.2. NM_019586.3.
UniGeneiMm.259095.

3D structure databases

ProteinModelPortaliQ9JJZ4.
SMRiQ9JJZ4. Positions 9-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207860. 1 interaction.
STRINGi10090.ENSMUSP00000118333.

PTM databases

PhosphoSiteiQ9JJZ4.

Proteomic databases

MaxQBiQ9JJZ4.
PaxDbiQ9JJZ4.
PRIDEiQ9JJZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124992; ENSMUSP00000118333; ENSMUSG00000028277.
GeneIDi56228.
KEGGimmu:56228.
UCSCiuc008sfp.1. mouse.

Organism-specific databases

CTDi51465.
MGIiMGI:1926245. Ube2j1.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00530000063379.
HOGENOMiHOG000167871.
HOVERGENiHBG058959.
InParanoidiQ9JJZ4.
KOiK10578.
OMAiDFCCEMC.
OrthoDBiEOG7HB59N.
PhylomeDBiQ9JJZ4.
TreeFamiTF101124.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiUbe2j1. mouse.
NextBioi312128.
PROiQ9JJZ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJZ4.
CleanExiMM_UBE2J1.
ExpressionAtlasiQ9JJZ4. baseline and differential.
GenevisibleiQ9JJZ4. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a family of non-canonical ubiquitin-conjugating enzymes structurally related to yeast UBC6."
    Lester D.H., Farquharson C., Russell G.C., Houston B.
    Biochem. Biophys. Res. Commun. 269:474-480(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUB2J1_MOUSE
AccessioniPrimary (citable) accession number: Q9JJZ4
Secondary accession number(s): Q9DC92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: September 13, 2004
Last modified: July 22, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.