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Q9JJY4

- DDX20_MOUSE

UniProt

Q9JJY4 - DDX20_MOUSE

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Protein

Probable ATP-dependent RNA helicase DDX20

Gene
Ddx20, Dp103, Gemin3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs) By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851ATP; via carbonyl oxygen By similarity
Binding sitei90 – 901ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1148ATP By similarity
Nucleotide bindingi110 – 1156ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. DNA binding Source: UniProtKB-KW
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  2. oogenesis Source: MGI
  3. positive regulation of apoptotic process Source: UniProtKB
  4. regulation of steroid biosynthetic process Source: MGI
  5. spliceosomal snRNP assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_206812. snRNP Assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX20 (EC:3.6.4.13)
Alternative name(s):
Component of gems 3
DEAD box protein 20
DEAD box protein DP 103
Gemin-3
Regulator of steroidogenic factor 1
Short name:
ROSF-1
Gene namesi
Name:Ddx20
Synonyms:Dp103, Gemin3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1858415. Ddx20.

Subcellular locationi

Cytoplasm. Nucleusgem
Note: Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. Gemini of coiled bodies Source: UniProtKB-SubCell
  3. SMN complex Source: UniProtKB
  4. SMN-Sm protein complex Source: UniProtKB
  5. transcriptional repressor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Probable ATP-dependent RNA helicase DDX20PRO_0000055026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881Phosphoserine By similarity
Modified residuei501 – 5011Phosphoserine By similarity
Modified residuei506 – 5061Phosphoserine By similarity
Modified residuei552 – 5521Phosphothreonine By similarity
Modified residuei653 – 6531Phosphoserine By similarity
Modified residuei655 – 6551Phosphoserine By similarity
Modified residuei657 – 6571Phosphoserine By similarity
Modified residuei673 – 6731Phosphoserine By similarity
Modified residuei678 – 6781Phosphoserine By similarity
Modified residuei679 – 6791Phosphoserine By similarity
Modified residuei689 – 6891Phosphothreonine By similarity
Modified residuei706 – 7061Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JJY4.
PaxDbiQ9JJY4.
PRIDEiQ9JJY4.

PTM databases

PhosphoSiteiQ9JJY4.

Expressioni

Gene expression databases

BgeeiQ9JJY4.
CleanExiMM_DDX20.
GenevestigatoriQ9JJY4.

Interactioni

Subunit structurei

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with GEMIN5. Interacts directly with SNUPN. Interacts with PPP4R2. Interacts with FOXL2. Interacts with NANOS1 and PUM2.2 Publications

Protein-protein interaction databases

BioGridi207545. 5 interactions.
IntActiQ9JJY4. 1 interaction.
STRINGi10090.ENSMUSP00000088176.

Structurei

3D structure databases

ProteinModelPortaliQ9JJY4.
SMRiQ9JJY4. Positions 6-443.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 265172Helicase ATP-bindingAdd
BLAST
Domaini300 – 449150Helicase C-terminalAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi63 – 9129Q motifAdd
BLAST
Motifi212 – 2154DEAD box

Sequence similaritiesi

Belongs to the DEAD box helicase family.

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00750000117579.
HOGENOMiHOG000112184.
HOVERGENiHBG051330.
InParanoidiQ059Z6.
KOiK13131.
OMAiQYYKIVN.
OrthoDBiEOG7PGDPX.
TreeFamiTF101524.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJY4-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAAFEVPA ALTTSESTMA AERAAAPVQA VEPTPASPWT QRTAHDIGGP    50
RTRTGDVVLA EPADFESLLL SRPVLEGLRA AGFERPSPVQ LKAIPLGRCG 100
LDLIVQAKSG TGKTCVFSTI ALDSLILENY STQILILAPT REIAVQIHSV 150
ITAIGIKMEG LECHVFIGGT PLSQDKTRLK KCHIAVGSPG RIKQLIELDY 200
LNPGSIRLFI LDEADKLLEE GSFQEQINWI YSSLPASKQM LAVSATYPEV 250
LANALTRYMR DPTFVRLNPS DPSLIGLKQY YQVVNSYPLA HKIFEEKTQH 300
LQELFSKVPF NQALVFSNLH SRAQHLADIL SSKGFPTECI SGNMNQNQRL 350
DAMAKLKQFH CRVLISTDLT SRGIDAEKVN LVVNLDVPLD WETYMHRIGR 400
AGRFGTLGLT VTYCCRGEEE NMMMKIAQKC NINLLPLPDP IPPGLMEECL 450
NWDVEVKAAM HTYSSPTVAT QSPKKQVQKL ERAFQSQRTP GNQTPSPRNT 500
SASALSARPK HSKPKLPVKS HSECGVLEKA APPQESGCPA QLEEQVKNSV 550
QTSVEDSSSN SQHQAKDSSP GSLPKIPCLS SFKVHQPSTL TFAELVDDYE 600
HYIKEGLEKP VEIIRHYTGP EAQTGNPQNG FVRNRVSEDR AQMLVSSSQS 650
GDSESDSDSC SSRTSSQSKG NKSYLEGSSD TQLKDTECTP VGGPLSLEQV 700
QNGNDTPTQV EYQEAPETQV KARHKEGANQ RSKQSRRNPA RRSSYRVQSE 750
PQEESWYDCH RETTASFSDT YQDYEEYWRA YYRAWQEYYA AASHSYYWNA 800
QRHPSWMAAY HMNTVYLQEM MRGNQ 825
Length:825
Mass (Da):91,710
Last modified:July 27, 2011 - v2
Checksum:iB5BCD0754D4075C3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51A → T in AAF76301. 1 Publication
Sequence conflicti9 – 91P → R in AAF76301. 1 Publication
Sequence conflicti226 – 2261Q → H in CAB86201. 1 Publication
Sequence conflicti468 – 4681V → I in AAF76301. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ250027 mRNA. Translation: CAB86201.1.
AF220454 mRNA. Translation: AAF76301.1.
CH466608 Genomic DNA. Translation: EDL07549.1.
BC125471 mRNA. Translation: AAI25472.1.
BC137721 mRNA. Translation: AAI37722.1.
CCDSiCCDS38581.1.
RefSeqiNP_059093.3. NM_017397.3.
XP_006501764.1. XM_006501701.1.
UniGeneiMm.272826.

Genome annotation databases

EnsembliENSMUST00000090680; ENSMUSP00000088176; ENSMUSG00000027905.
GeneIDi53975.
KEGGimmu:53975.
UCSCiuc008qva.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ250027 mRNA. Translation: CAB86201.1 .
AF220454 mRNA. Translation: AAF76301.1 .
CH466608 Genomic DNA. Translation: EDL07549.1 .
BC125471 mRNA. Translation: AAI25472.1 .
BC137721 mRNA. Translation: AAI37722.1 .
CCDSi CCDS38581.1.
RefSeqi NP_059093.3. NM_017397.3.
XP_006501764.1. XM_006501701.1.
UniGenei Mm.272826.

3D structure databases

ProteinModelPortali Q9JJY4.
SMRi Q9JJY4. Positions 6-443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207545. 5 interactions.
IntActi Q9JJY4. 1 interaction.
STRINGi 10090.ENSMUSP00000088176.

PTM databases

PhosphoSitei Q9JJY4.

Proteomic databases

MaxQBi Q9JJY4.
PaxDbi Q9JJY4.
PRIDEi Q9JJY4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000090680 ; ENSMUSP00000088176 ; ENSMUSG00000027905 .
GeneIDi 53975.
KEGGi mmu:53975.
UCSCi uc008qva.1. mouse.

Organism-specific databases

CTDi 11218.
MGIi MGI:1858415. Ddx20.

Phylogenomic databases

eggNOGi COG0513.
GeneTreei ENSGT00750000117579.
HOGENOMi HOG000112184.
HOVERGENi HBG051330.
InParanoidi Q059Z6.
KOi K13131.
OMAi QYYKIVN.
OrthoDBi EOG7PGDPX.
TreeFami TF101524.

Enzyme and pathway databases

Reactomei REACT_206812. snRNP Assembly.

Miscellaneous databases

NextBioi 310865.
PROi Q9JJY4.
SOURCEi Search...

Gene expression databases

Bgeei Q9JJY4.
CleanExi MM_DDX20.
Genevestigatori Q9JJY4.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Direct interaction of Smn with dp103, a putative RNA helicase: a role for Smn in transcription regulation?"
    Campbell L., Hunter K.M., Mohaghegh P., Tinsley J.M., Brasch M.A., Davies K.E.
    Hum. Mol. Genet. 9:1093-1100(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SMN1.
  2. "The DEAD box protein DP103 is a regulator of steroidogenic factor-1."
    Ou Q., Mouillet J.F., Yan X., Dorn C., Crawford P.A., Sadovsky Y.
    Mol. Endocrinol. 15:69-79(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Testis.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  5. "Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis."
    Lee K., Pisarska M.D., Ko J.J., Kang Y., Yoon S., Ryou S.M., Cha K.Y., Bae J.
    Biochem. Biophys. Res. Commun. 336:876-881(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FOXL2.

Entry informationi

Entry nameiDDX20_MOUSE
AccessioniPrimary (citable) accession number: Q9JJY4
Secondary accession number(s): Q059Z6, Q9JIK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi