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Q9JJY4 (DDX20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DDX20

EC=3.6.4.13
Alternative name(s):
Component of gems 3
DEAD box protein 20
DEAD box protein DP 103
Gemin-3
Regulator of steroidogenic factor 1
Short name=ROSF-1
Gene names
Name:Ddx20
Synonyms:Dp103, Gemin3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length825 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs) By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with GEMIN5. Interacts directly with SNUPN. Interacts with PPP4R2. Interacts with FOXL2. Interacts with NANOS1 and PUM2. Ref.1 Ref.5

Subcellular location

Cytoplasm. Nucleusgem. Note: Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

Sequence similarities

Belongs to the DEAD box helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 825825Probable ATP-dependent RNA helicase DDX20
PRO_0000055026

Regions

Domain94 – 265172Helicase ATP-binding
Domain300 – 449150Helicase C-terminal
Nucleotide binding107 – 1148ATP By similarity
Nucleotide binding110 – 1156ATP By similarity
Motif63 – 9129Q motif
Motif212 – 2154DEAD box

Sites

Binding site851ATP; via carbonyl oxygen By similarity
Binding site901ATP By similarity

Amino acid modifications

Modified residue1881Phosphoserine By similarity
Modified residue5011Phosphoserine By similarity
Modified residue5061Phosphoserine By similarity
Modified residue5521Phosphothreonine By similarity
Modified residue6531Phosphoserine By similarity
Modified residue6551Phosphoserine By similarity
Modified residue6571Phosphoserine By similarity
Modified residue6731Phosphoserine By similarity
Modified residue6781Phosphoserine By similarity
Modified residue6791Phosphoserine By similarity
Modified residue6891Phosphothreonine By similarity
Modified residue7061Phosphothreonine By similarity

Experimental info

Sequence conflict51A → T in AAF76301. Ref.2
Sequence conflict91P → R in AAF76301. Ref.2
Sequence conflict2261Q → H in CAB86201. Ref.1
Sequence conflict4681V → I in AAF76301. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9JJY4 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: B5BCD0754D4075C3

FASTA82591,710
        10         20         30         40         50         60 
MAAAAFEVPA ALTTSESTMA AERAAAPVQA VEPTPASPWT QRTAHDIGGP RTRTGDVVLA 

        70         80         90        100        110        120 
EPADFESLLL SRPVLEGLRA AGFERPSPVQ LKAIPLGRCG LDLIVQAKSG TGKTCVFSTI 

       130        140        150        160        170        180 
ALDSLILENY STQILILAPT REIAVQIHSV ITAIGIKMEG LECHVFIGGT PLSQDKTRLK 

       190        200        210        220        230        240 
KCHIAVGSPG RIKQLIELDY LNPGSIRLFI LDEADKLLEE GSFQEQINWI YSSLPASKQM 

       250        260        270        280        290        300 
LAVSATYPEV LANALTRYMR DPTFVRLNPS DPSLIGLKQY YQVVNSYPLA HKIFEEKTQH 

       310        320        330        340        350        360 
LQELFSKVPF NQALVFSNLH SRAQHLADIL SSKGFPTECI SGNMNQNQRL DAMAKLKQFH 

       370        380        390        400        410        420 
CRVLISTDLT SRGIDAEKVN LVVNLDVPLD WETYMHRIGR AGRFGTLGLT VTYCCRGEEE 

       430        440        450        460        470        480 
NMMMKIAQKC NINLLPLPDP IPPGLMEECL NWDVEVKAAM HTYSSPTVAT QSPKKQVQKL 

       490        500        510        520        530        540 
ERAFQSQRTP GNQTPSPRNT SASALSARPK HSKPKLPVKS HSECGVLEKA APPQESGCPA 

       550        560        570        580        590        600 
QLEEQVKNSV QTSVEDSSSN SQHQAKDSSP GSLPKIPCLS SFKVHQPSTL TFAELVDDYE 

       610        620        630        640        650        660 
HYIKEGLEKP VEIIRHYTGP EAQTGNPQNG FVRNRVSEDR AQMLVSSSQS GDSESDSDSC 

       670        680        690        700        710        720 
SSRTSSQSKG NKSYLEGSSD TQLKDTECTP VGGPLSLEQV QNGNDTPTQV EYQEAPETQV 

       730        740        750        760        770        780 
KARHKEGANQ RSKQSRRNPA RRSSYRVQSE PQEESWYDCH RETTASFSDT YQDYEEYWRA 

       790        800        810        820 
YYRAWQEYYA AASHSYYWNA QRHPSWMAAY HMNTVYLQEM MRGNQ 

« Hide

References

« Hide 'large scale' references
[1]"Direct interaction of Smn with dp103, a putative RNA helicase: a role for Smn in transcription regulation?"
Campbell L., Hunter K.M., Mohaghegh P., Tinsley J.M., Brasch M.A., Davies K.E.
Hum. Mol. Genet. 9:1093-1100(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SMN1.
[2]"The DEAD box protein DP103 is a regulator of steroidogenic factor-1."
Ou Q., Mouillet J.F., Yan X., Dorn C., Crawford P.A., Sadovsky Y.
Mol. Endocrinol. 15:69-79(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Testis.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[5]"Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis."
Lee K., Pisarska M.D., Ko J.J., Kang Y., Yoon S., Ryou S.M., Cha K.Y., Bae J.
Biochem. Biophys. Res. Commun. 336:876-881(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FOXL2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ250027 mRNA. Translation: CAB86201.1.
AF220454 mRNA. Translation: AAF76301.1.
CH466608 Genomic DNA. Translation: EDL07549.1.
BC125471 mRNA. Translation: AAI25472.1.
BC137721 mRNA. Translation: AAI37722.1.
RefSeqNP_059093.3. NM_017397.3.
XP_006501764.1. XM_006501701.1.
UniGeneMm.272826.

3D structure databases

ProteinModelPortalQ9JJY4.
SMRQ9JJY4. Positions 63-442.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207545. 5 interactions.
IntActQ9JJY4. 1 interaction.
STRING10090.ENSMUSP00000088176.

PTM databases

PhosphoSiteQ9JJY4.

Proteomic databases

PaxDbQ9JJY4.
PRIDEQ9JJY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090680; ENSMUSP00000088176; ENSMUSG00000027905.
GeneID53975.
KEGGmmu:53975.
UCSCuc008qva.1. mouse.

Organism-specific databases

CTD11218.
MGIMGI:1858415. Ddx20.

Phylogenomic databases

eggNOGCOG0513.
GeneTreeENSGT00750000117579.
HOGENOMHOG000112184.
HOVERGENHBG051330.
InParanoidQ059Z6.
KOK13131.
OMASECGIIE.
OrthoDBEOG7PGDPX.
TreeFamTF101524.

Gene expression databases

BgeeQ9JJY4.
CleanExMM_DDX20.
GenevestigatorQ9JJY4.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio310865.
PROQ9JJY4.
SOURCESearch...

Entry information

Entry nameDDX20_MOUSE
AccessionPrimary (citable) accession number: Q9JJY4
Secondary accession number(s): Q059Z6, Q9JIK4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot