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Q9JJY4

- DDX20_MOUSE

UniProt

Q9JJY4 - DDX20_MOUSE

Protein

Probable ATP-dependent RNA helicase DDX20

Gene

Ddx20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs) By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851ATP; via carbonyl oxygenBy similarity
    Binding sitei90 – 901ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi107 – 1148ATPPROSITE-ProRule annotation
    Nucleotide bindingi110 – 1156ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. DNA binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    2. oogenesis Source: MGI
    3. positive regulation of apoptotic process Source: UniProtKB
    4. regulation of steroid biosynthetic process Source: MGI
    5. spliceosomal snRNP assembly Source: UniProtKB

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_206812. snRNP Assembly.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DDX20 (EC:3.6.4.13)
    Alternative name(s):
    Component of gems 3
    DEAD box protein 20
    DEAD box protein DP 103
    Gemin-3
    Regulator of steroidogenic factor 1
    Short name:
    ROSF-1
    Gene namesi
    Name:Ddx20
    Synonyms:Dp103, Gemin3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1858415. Ddx20.

    Subcellular locationi

    Cytoplasm. Nucleusgem
    Note: Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. Gemini of coiled bodies Source: UniProtKB-SubCell
    3. SMN complex Source: UniProtKB
    4. SMN-Sm protein complex Source: UniProtKB
    5. transcriptional repressor complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 825825Probable ATP-dependent RNA helicase DDX20PRO_0000055026Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881PhosphoserineBy similarity
    Modified residuei501 – 5011PhosphoserineBy similarity
    Modified residuei506 – 5061PhosphoserineBy similarity
    Modified residuei552 – 5521PhosphothreonineBy similarity
    Modified residuei653 – 6531PhosphoserineBy similarity
    Modified residuei655 – 6551PhosphoserineBy similarity
    Modified residuei657 – 6571PhosphoserineBy similarity
    Modified residuei673 – 6731PhosphoserineBy similarity
    Modified residuei678 – 6781PhosphoserineBy similarity
    Modified residuei679 – 6791PhosphoserineBy similarity
    Modified residuei689 – 6891PhosphothreonineBy similarity
    Modified residuei706 – 7061PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9JJY4.
    PaxDbiQ9JJY4.
    PRIDEiQ9JJY4.

    PTM databases

    PhosphoSiteiQ9JJY4.

    Expressioni

    Gene expression databases

    BgeeiQ9JJY4.
    CleanExiMM_DDX20.
    GenevestigatoriQ9JJY4.

    Interactioni

    Subunit structurei

    Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with GEMIN5. Interacts directly with SNUPN. Interacts with PPP4R2. Interacts with FOXL2. Interacts with NANOS1 and PUM2.2 Publications

    Protein-protein interaction databases

    BioGridi207545. 5 interactions.
    IntActiQ9JJY4. 1 interaction.
    STRINGi10090.ENSMUSP00000088176.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JJY4.
    SMRiQ9JJY4. Positions 6-443.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini94 – 265172Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini300 – 449150Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi63 – 9129Q motifAdd
    BLAST
    Motifi212 – 2154DEAD box

    Sequence similaritiesi

    Belongs to the DEAD box helicase family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    GeneTreeiENSGT00750000117579.
    HOGENOMiHOG000112184.
    HOVERGENiHBG051330.
    InParanoidiQ059Z6.
    KOiK13131.
    OMAiQYYKIVN.
    OrthoDBiEOG7PGDPX.
    TreeFamiTF101524.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JJY4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAAFEVPA ALTTSESTMA AERAAAPVQA VEPTPASPWT QRTAHDIGGP    50
    RTRTGDVVLA EPADFESLLL SRPVLEGLRA AGFERPSPVQ LKAIPLGRCG 100
    LDLIVQAKSG TGKTCVFSTI ALDSLILENY STQILILAPT REIAVQIHSV 150
    ITAIGIKMEG LECHVFIGGT PLSQDKTRLK KCHIAVGSPG RIKQLIELDY 200
    LNPGSIRLFI LDEADKLLEE GSFQEQINWI YSSLPASKQM LAVSATYPEV 250
    LANALTRYMR DPTFVRLNPS DPSLIGLKQY YQVVNSYPLA HKIFEEKTQH 300
    LQELFSKVPF NQALVFSNLH SRAQHLADIL SSKGFPTECI SGNMNQNQRL 350
    DAMAKLKQFH CRVLISTDLT SRGIDAEKVN LVVNLDVPLD WETYMHRIGR 400
    AGRFGTLGLT VTYCCRGEEE NMMMKIAQKC NINLLPLPDP IPPGLMEECL 450
    NWDVEVKAAM HTYSSPTVAT QSPKKQVQKL ERAFQSQRTP GNQTPSPRNT 500
    SASALSARPK HSKPKLPVKS HSECGVLEKA APPQESGCPA QLEEQVKNSV 550
    QTSVEDSSSN SQHQAKDSSP GSLPKIPCLS SFKVHQPSTL TFAELVDDYE 600
    HYIKEGLEKP VEIIRHYTGP EAQTGNPQNG FVRNRVSEDR AQMLVSSSQS 650
    GDSESDSDSC SSRTSSQSKG NKSYLEGSSD TQLKDTECTP VGGPLSLEQV 700
    QNGNDTPTQV EYQEAPETQV KARHKEGANQ RSKQSRRNPA RRSSYRVQSE 750
    PQEESWYDCH RETTASFSDT YQDYEEYWRA YYRAWQEYYA AASHSYYWNA 800
    QRHPSWMAAY HMNTVYLQEM MRGNQ 825
    Length:825
    Mass (Da):91,710
    Last modified:July 27, 2011 - v2
    Checksum:iB5BCD0754D4075C3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51A → T in AAF76301. (PubMed:11145740)Curated
    Sequence conflicti9 – 91P → R in AAF76301. (PubMed:11145740)Curated
    Sequence conflicti226 – 2261Q → H in CAB86201. (PubMed:10767334)Curated
    Sequence conflicti468 – 4681V → I in AAF76301. (PubMed:11145740)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ250027 mRNA. Translation: CAB86201.1.
    AF220454 mRNA. Translation: AAF76301.1.
    CH466608 Genomic DNA. Translation: EDL07549.1.
    BC125471 mRNA. Translation: AAI25472.1.
    BC137721 mRNA. Translation: AAI37722.1.
    CCDSiCCDS38581.1.
    RefSeqiNP_059093.3. NM_017397.3.
    XP_006501764.1. XM_006501701.1.
    UniGeneiMm.272826.

    Genome annotation databases

    EnsembliENSMUST00000090680; ENSMUSP00000088176; ENSMUSG00000027905.
    GeneIDi53975.
    KEGGimmu:53975.
    UCSCiuc008qva.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ250027 mRNA. Translation: CAB86201.1 .
    AF220454 mRNA. Translation: AAF76301.1 .
    CH466608 Genomic DNA. Translation: EDL07549.1 .
    BC125471 mRNA. Translation: AAI25472.1 .
    BC137721 mRNA. Translation: AAI37722.1 .
    CCDSi CCDS38581.1.
    RefSeqi NP_059093.3. NM_017397.3.
    XP_006501764.1. XM_006501701.1.
    UniGenei Mm.272826.

    3D structure databases

    ProteinModelPortali Q9JJY4.
    SMRi Q9JJY4. Positions 6-443.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207545. 5 interactions.
    IntActi Q9JJY4. 1 interaction.
    STRINGi 10090.ENSMUSP00000088176.

    PTM databases

    PhosphoSitei Q9JJY4.

    Proteomic databases

    MaxQBi Q9JJY4.
    PaxDbi Q9JJY4.
    PRIDEi Q9JJY4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000090680 ; ENSMUSP00000088176 ; ENSMUSG00000027905 .
    GeneIDi 53975.
    KEGGi mmu:53975.
    UCSCi uc008qva.1. mouse.

    Organism-specific databases

    CTDi 11218.
    MGIi MGI:1858415. Ddx20.

    Phylogenomic databases

    eggNOGi COG0513.
    GeneTreei ENSGT00750000117579.
    HOGENOMi HOG000112184.
    HOVERGENi HBG051330.
    InParanoidi Q059Z6.
    KOi K13131.
    OMAi QYYKIVN.
    OrthoDBi EOG7PGDPX.
    TreeFami TF101524.

    Enzyme and pathway databases

    Reactomei REACT_206812. snRNP Assembly.

    Miscellaneous databases

    NextBioi 310865.
    PROi Q9JJY4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JJY4.
    CleanExi MM_DDX20.
    Genevestigatori Q9JJY4.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Direct interaction of Smn with dp103, a putative RNA helicase: a role for Smn in transcription regulation?"
      Campbell L., Hunter K.M., Mohaghegh P., Tinsley J.M., Brasch M.A., Davies K.E.
      Hum. Mol. Genet. 9:1093-1100(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SMN1.
    2. "The DEAD box protein DP103 is a regulator of steroidogenic factor-1."
      Ou Q., Mouillet J.F., Yan X., Dorn C., Crawford P.A., Sadovsky Y.
      Mol. Endocrinol. 15:69-79(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Testis.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    5. "Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis."
      Lee K., Pisarska M.D., Ko J.J., Kang Y., Yoon S., Ryou S.M., Cha K.Y., Bae J.
      Biochem. Biophys. Res. Commun. 336:876-881(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FOXL2.

    Entry informationi

    Entry nameiDDX20_MOUSE
    AccessioniPrimary (citable) accession number: Q9JJY4
    Secondary accession number(s): Q059Z6, Q9JIK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3