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Protein

P2X purinoceptor 4

Gene

P2rx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. cadherin binding Source: MGI
  3. extracellular ATP-gated cation channel activity Source: MGI
  4. purinergic nucleotide receptor activity Source: MGI

GO - Biological processi

  1. apoptotic signaling pathway Source: MGI
  2. calcium ion transport Source: MGI
  3. cation transmembrane transport Source: GOC
  4. cellular response to ATP Source: UniProtKB
  5. ion transmembrane transport Source: MGI
  6. membrane depolarization Source: MGI
  7. negative regulation of cardiac muscle hypertrophy Source: MGI
  8. nitric oxide biosynthetic process Source: MGI
  9. positive regulation of calcium ion transport into cytosol Source: MGI
  10. positive regulation of calcium-mediated signaling Source: MGI
  11. purinergic nucleotide receptor signaling pathway Source: MGI
  12. regulation of blood pressure Source: MGI
  13. regulation of cardiac muscle contraction Source: MGI
  14. regulation of excitatory postsynaptic membrane potential Source: MGI
  15. relaxation of cardiac muscle Source: MGI
  16. response to ATP Source: MGI
  17. response to fluid shear stress Source: MGI
  18. signal transduction Source: MGI
  19. transport Source: MGI
  20. vasodilation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 4
Short name:
P2X4
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2rx4
Synonyms:P2x4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1338859. P2rx4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei34 – 5421Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini55 – 338284ExtracellularSequence AnalysisAdd
BLAST
Transmembranei339 – 35921Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini360 – 38829CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical part of cell Source: MGI
  2. cell junction Source: MGI
  3. extracellular vesicular exosome Source: MGI
  4. integral component of nuclear inner membrane Source: GO_Central
  5. integral component of plasma membrane Source: UniProtKB
  6. lysosomal membrane Source: MGI
  7. membrane Source: MGI
  8. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388P2X purinoceptor 4PRO_0000269194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi116 ↔ 165By similarity
Disulfide bondi126 ↔ 149By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi132 ↔ 159By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi217 ↔ 227By similarity
Disulfide bondi261 ↔ 270By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JJX6.
PaxDbiQ9JJX6.
PRIDEiQ9JJX6.

PTM databases

PhosphoSiteiQ9JJX6.

Expressioni

Gene expression databases

GenevestigatoriQ9JJX6.

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9JJX6.
SMRiQ9JJX6. Positions 31-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47843.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ9JJX6.
PhylomeDBiQ9JJX6.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9JJX6-1) [UniParc]FASTAAdd to basket

Also known as: P2X4a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRVVQLLIL AYVIGWVFVW
60 70 80 90 100
EKGYQETDSV VSSVTTKAKG VAVTNTSQLG FRIWDVADYV VPAQEENSLF
110 120 130 140 150
IMTNMIVTVN QTQGTCPEIP DKTSICDSDA NCTLGSSDTH SSGIGTGRCV
160 170 180 190 200
PFNASVKTCE VAAWCPVEND AGVPTPAFLK AAENFTLLVK NNIWYPKFNF
210 220 230 240 250
SKRNILPNIT TSYLKSCIYN ARTDPFCPIF RLGQIVADAG HSFQEMAVEG
260 270 280 290 300
GIMGIQIKWD CNLDRAASHC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY
310 320 330 340 350
RDLAGNEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT
360 370 380
VLCDVIVLYC MKKRYYYRDK KYKYVEDYEQ GLSGEMNQ
Length:388
Mass (Da):43,438
Last modified:October 1, 2000 - v1
Checksum:i4F388F006DB6DF45
GO
Isoform b (identifier: Q9JJX6-2) [UniParc]FASTAAdd to basket

Also known as: P2X4b

The sequence of this isoform differs from the canonical sequence as follows:
     176-202: Missing.

Show »
Length:361
Mass (Da):40,254
Checksum:iA8930EAACBDB0F8C
GO
Isoform c (identifier: Q9JJX6-3) [UniParc]FASTAAdd to basket

Also known as: P2X4c

The sequence of this isoform differs from the canonical sequence as follows:
     328-349: Missing.

Show »
Length:366
Mass (Da):41,269
Checksum:i0762FA5FB21670B1
GO
Isoform d (identifier: Q9JJX6-4) [UniParc]FASTAAdd to basket

Also known as: P2X4d

The sequence of this isoform differs from the canonical sequence as follows:
     176-202: Missing.
     328-349: Missing.

Show »
Length:339
Mass (Da):38,086
Checksum:iF780AA6A300A1E77
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei176 – 20227Missing in isoform b and isoform d. 1 PublicationVSP_022023Add
BLAST
Alternative sequencei328 – 34922Missing in isoform c and isoform d. 1 PublicationVSP_022024Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251459 mRNA. Translation: CAB90749.1.
AJ251460 mRNA. Translation: CAB90750.1.
AJ251461 mRNA. Translation: CAB90751.1.
AJ251462 mRNA. Translation: CAB90752.1.
CCDSiCCDS19654.2. [Q9JJX6-1]
UniGeneiMm.290884.
Mm.463383.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251459 mRNA. Translation: CAB90749.1.
AJ251460 mRNA. Translation: CAB90750.1.
AJ251461 mRNA. Translation: CAB90751.1.
AJ251462 mRNA. Translation: CAB90752.1.
CCDSiCCDS19654.2. [Q9JJX6-1]
UniGeneiMm.290884.
Mm.463383.

3D structure databases

ProteinModelPortaliQ9JJX6.
SMRiQ9JJX6. Positions 31-358.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ9JJX6.
ChEMBLiCHEMBL2176849.
GuidetoPHARMACOLOGYi481.

PTM databases

PhosphoSiteiQ9JJX6.

Proteomic databases

MaxQBiQ9JJX6.
PaxDbiQ9JJX6.
PRIDEiQ9JJX6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1338859. P2rx4.

Phylogenomic databases

eggNOGiNOG47843.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ9JJX6.
PhylomeDBiQ9JJX6.

Miscellaneous databases

PROiQ9JJX6.
SOURCEiSearch...

Gene expression databases

GenevestigatoriQ9JJX6.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Alternatively spliced variants of the mouse P2X4 receptor: cloning and characterisation."
    Simon J., Michel A.D., Chessell I.P., Kidd E.J., Jones C.A., Barnard E.A., Humphrey P.P.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D).

Entry informationi

Entry nameiP2RX4_MOUSE
AccessioniPrimary (citable) accession number: Q9JJX6
Secondary accession number(s): Q9JJX3, Q9JJX4, Q9JJX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.