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Q9JJX6 (P2RX4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
P2X purinoceptor 4

Short name=P2X4
Alternative name(s):
ATP receptor
Purinergic receptor
Gene names
Name:P2rx4
Synonyms:P2x4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin By similarity.

Subunit structure

Functional P2XRs are organized as homomeric and heteromeric trimers By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the P2X receptor family.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium ion transport

Inferred from mutant phenotype PubMed 16327800. Source: MGI

cellular response to ATP

Inferred from sequence or structural similarity. Source: UniProtKB

nitric oxide biosynthetic process

Inferred from direct assay PubMed 16327800. Source: MGI

purinergic nucleotide receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: GOC

regulation of excitatory postsynaptic membrane potential

Inferred from mutant phenotype PubMed 16943557. Source: MGI

vasodilation

Inferred from mutant phenotype PubMed 16327800. Source: MGI

   Cellular_componentapical part of cell

Inferred from direct assay PubMed 17560948. Source: MGI

integral component of nuclear inner membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

extracellular ATP-gated cation channel activity

Inferred from mutant phenotype PubMed 15081800. Source: MGI

purinergic nucleotide receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q9JJX6-1)

Also known as: P2X4a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q9JJX6-2)

Also known as: P2X4b;

The sequence of this isoform differs from the canonical sequence as follows:
     176-202: Missing.
Isoform c (identifier: Q9JJX6-3)

Also known as: P2X4c;

The sequence of this isoform differs from the canonical sequence as follows:
     328-349: Missing.
Isoform d (identifier: Q9JJX6-4)

Also known as: P2X4d;

The sequence of this isoform differs from the canonical sequence as follows:
     176-202: Missing.
     328-349: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388P2X purinoceptor 4
PRO_0000269194

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421Helical; Name=1; Potential
Topological domain55 – 338284Extracellular Potential
Transmembrane339 – 35921Helical; Name=2; Potential
Topological domain360 – 38829Cytoplasmic Potential

Amino acid modifications

Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation1991N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Disulfide bond116 ↔ 165 By similarity
Disulfide bond126 ↔ 149 By similarity
Disulfide bond132 ↔ 159 By similarity
Disulfide bond217 ↔ 227 By similarity
Disulfide bond261 ↔ 270 By similarity

Natural variations

Alternative sequence176 – 20227Missing in isoform b and isoform d.
VSP_022023
Alternative sequence328 – 34922Missing in isoform c and isoform d.
VSP_022024

Sequences

Sequence LengthMass (Da)Tools
Isoform a (P2X4a) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 4F388F006DB6DF45

FASTA38843,438
        10         20         30         40         50         60 
MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRVVQLLIL AYVIGWVFVW EKGYQETDSV 

        70         80         90        100        110        120 
VSSVTTKAKG VAVTNTSQLG FRIWDVADYV VPAQEENSLF IMTNMIVTVN QTQGTCPEIP 

       130        140        150        160        170        180 
DKTSICDSDA NCTLGSSDTH SSGIGTGRCV PFNASVKTCE VAAWCPVEND AGVPTPAFLK 

       190        200        210        220        230        240 
AAENFTLLVK NNIWYPKFNF SKRNILPNIT TSYLKSCIYN ARTDPFCPIF RLGQIVADAG 

       250        260        270        280        290        300 
HSFQEMAVEG GIMGIQIKWD CNLDRAASHC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY 

       310        320        330        340        350        360 
RDLAGNEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT VLCDVIVLYC 

       370        380 
MKKRYYYRDK KYKYVEDYEQ GLSGEMNQ 

« Hide

Isoform b (P2X4b) [UniParc].

Checksum: A8930EAACBDB0F8C
Show »

FASTA36140,254
Isoform c (P2X4c) [UniParc].

Checksum: 0762FA5FB21670B1
Show »

FASTA36641,269
Isoform d (P2X4d) [UniParc].

Checksum: F780AA6A300A1E77
Show »

FASTA33938,086

References

[1]"Alternatively spliced variants of the mouse P2X4 receptor: cloning and characterisation."
Simon J., Michel A.D., Chessell I.P., Kidd E.J., Jones C.A., Barnard E.A., Humphrey P.P.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ251459 mRNA. Translation: CAB90749.1.
AJ251460 mRNA. Translation: CAB90750.1.
AJ251461 mRNA. Translation: CAB90751.1.
AJ251462 mRNA. Translation: CAB90752.1.
CCDSCCDS19654.2. [Q9JJX6-1]
UniGeneMm.290884.
Mm.463383.

3D structure databases

ProteinModelPortalQ9JJX6.
SMRQ9JJX6. Positions 31-358.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2176849.
GuidetoPHARMACOLOGY481.

PTM databases

PhosphoSiteQ9JJX6.

Proteomic databases

MaxQBQ9JJX6.
PaxDbQ9JJX6.
PRIDEQ9JJX6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:1338859. P2rx4.

Phylogenomic databases

eggNOGNOG47843.
HOGENOMHOG000232042.
HOVERGENHBG053086.
InParanoidQ9JJX6.
PhylomeDBQ9JJX6.

Gene expression databases

GenevestigatorQ9JJX6.

Family and domain databases

Gene3D2.60.490.10. 1 hit.
InterProIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamPF00864. P2X_receptor. 1 hit.
[Graphical view]
PRINTSPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsTIGR00863. P2X. 1 hit.
PROSITEPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSP2RX4. mouse.
PROQ9JJX6.
SOURCESearch...

Entry information

Entry nameP2RX4_MOUSE
AccessionPrimary (citable) accession number: Q9JJX6
Secondary accession number(s): Q9JJX3, Q9JJX4, Q9JJX5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot