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Protein

Phosphorylated adapter RNA export protein

Gene

Phax

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • snRNA export from nucleus Source: MGI

Keywordsi

Molecular functionRNA-binding
Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylated adapter RNA export protein
Alternative name(s):
RNA U small nuclear RNA export adapter protein
Gene namesi
Name:Phax
Synonyms:Rnuxa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1891839. Phax.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleoplasm By similarity
  • NucleusCajal body By similarity
  • Cytoplasm 1 Publication

  • Note: Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.By similarity

GO - Cellular componenti

  • Cajal body Source: UniProtKB-SubCell
  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • neuronal cell body Source: Ensembl
  • nucleoplasm Source: MGI
  • nucleus Source: MGI

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71 – 74KRKR → ARAA: Strongly reduces its import in the nucleus. Abolishes its import in the nucleus, does not change its export to the cytoplasm and the export of U snRNA complex; when associated with 190-AA-191. 1 Publication4
Mutagenesisi124 – 129LGILGM → AGIAGA: Reduces U snRNA export. Strongly abolishes the U snRNA export complex formation. Does not abolish the pre-complex formation. 1 Publication6
Mutagenesisi190 – 191RK → AA: Strongly reduces its import in the nucleus. Abolishes its import in the nucleus, does not change its export to the cytoplasm and the export of the U snRNA complex; when associated with 71-ARAA-74. 1 Publication2
Mutagenesisi255 – 258ETAE → AAAA: Does not inhibit U RNA-binding. 1 Publication4
Mutagenesisi260 – 262EQN → AAA: Partially inhibits U RNA-binding. 1 Publication3
Mutagenesisi263 – 264GG → AA: Strongly inhibits U RNA-binding. 1 Publication2
Mutagenesisi270 – 271GS → AA: Partially inhibits U RNA-binding. 1 Publication2
Mutagenesisi273 – 274RR → AA: Partially reduces its import in the nucleus. 1 Publication2
Mutagenesisi275 – 276TP → AA: Partially inhibits U RNA-binding. 1 Publication2
Mutagenesisi289 – 293SISEE → AIAEA: Does not inhibit U RNA-binding. 1 Publication5
Mutagenesisi315 – 316KR → AA: Partially reduces its import in the nucleus. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002397762 – 385Phosphorylated adapter RNA export proteinAdd BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei14PhosphoserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei56PhosphoserineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei63PhosphoserineBy similarity1
Modified residuei287PhosphothreonineBy similarity1
Modified residuei347PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JJT9.
MaxQBiQ9JJT9.
PaxDbiQ9JJT9.
PRIDEiQ9JJT9.

PTM databases

iPTMnetiQ9JJT9.
PhosphoSitePlusiQ9JJT9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000008301.
ExpressionAtlasiQ9JJT9. baseline and differential.
GenevisibleiQ9JJT9. MM.

Interactioni

Subunit structurei

Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20 and m7G-capped RNA. Interacts with NCBP1/CBP80. Found in a complex with snoRNA. Interacts with NCBP2/CBP20 (By similarity).By similarity2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000008445.

Structurei

3D structure databases

ProteinModelPortaliQ9JJT9.
SMRiQ9JJT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 320Necessary for interaction with CBP80Add BLAST319
Regioni219 – 319Sufficient for poly U RNA-bindingAdd BLAST101
Regioni270 – 278Necessary for poly U RNA-binding and snRNA export9

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 74Nuclear localization signal4
Motifi120 – 129Nuclear export signal10
Motifi189 – 192Nuclear localization signal4

Sequence similaritiesi

Belongs to the PHAX family.Curated

Phylogenomic databases

eggNOGiKOG3948. Eukaryota.
ENOG4110D9C. LUCA.
GeneTreeiENSGT00390000011084.
HOGENOMiHOG000115525.
HOVERGENiHBG058976.
InParanoidiQ9JJT9.
KOiK14291.
OMAiSQEHTKE.
OrthoDBiEOG091G0XKP.
PhylomeDBiQ9JJT9.
TreeFamiTF321050.

Family and domain databases

InterProiView protein in InterPro
IPR019385. PHAX_RNA-binding_domain.
PfamiView protein in Pfam
PF10258. RNA_GG_bind. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JJT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEAGDMEE GQLSDSDSDM TVVPSDRPLQ MAKVLGGGSA ACAPVSHYRT
60 70 80 90 100
VKHVDSSEES LDSDDDCSLW KRKRQKCHNT PPKPEPFPFG PSGQKTALNG
110 120 130 140 150
GKKVNNIWGA VLQEQNQDAV ATELGILGME GSIDRSRQSE TYNYLLAKKL
160 170 180 190 200
AKKESQEYTK ELDKDLDEYM HGDKKPGSKE DENGQGHLKR KRPVRDRLGN
210 220 230 240 250
RVEMNYKGRY EITEEDAPEK VADEIAFRLQ EPKKDLIARV VRILGNKKAI
260 270 280 290 300
ELLMETAEVE QNGGLFIMNG SRRRTPGGVF LNLLKNTPSI SEEQIKDIFY
310 320 330 340 350
VENQKEYENK KAARKRRTQL LGKKMKQAIK SLNFQEDDDT SRETFASDTN
360 370 380
EALASLDEAQ EGPGETKLDA EEAIEVDHPQ DLDIF
Length:385
Mass (Da):43,247
Last modified:October 1, 2000 - v1
Checksum:i90946BE46B7ABDCA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236L → Q in BAC27117 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276504 mRNA. Translation: CAB87994.1.
AK030749 mRNA. Translation: BAC27117.1.
BC049590 mRNA. Translation: AAH49590.1.
BC061110 mRNA. Translation: AAH61110.1.
CCDSiCCDS29259.1.
RefSeqiNP_001156461.1. NM_001162989.1.
NP_064380.3. NM_019996.4.
UniGeneiMm.214569.

Genome annotation databases

EnsembliENSMUST00000008445; ENSMUSP00000008445; ENSMUSG00000008301.
GeneIDi56698.
KEGGimmu:56698.
UCSCiuc008eyq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276504 mRNA. Translation: CAB87994.1.
AK030749 mRNA. Translation: BAC27117.1.
BC049590 mRNA. Translation: AAH49590.1.
BC061110 mRNA. Translation: AAH61110.1.
CCDSiCCDS29259.1.
RefSeqiNP_001156461.1. NM_001162989.1.
NP_064380.3. NM_019996.4.
UniGeneiMm.214569.

3D structure databases

ProteinModelPortaliQ9JJT9.
SMRiQ9JJT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000008445.

PTM databases

iPTMnetiQ9JJT9.
PhosphoSitePlusiQ9JJT9.

Proteomic databases

EPDiQ9JJT9.
MaxQBiQ9JJT9.
PaxDbiQ9JJT9.
PRIDEiQ9JJT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008445; ENSMUSP00000008445; ENSMUSG00000008301.
GeneIDi56698.
KEGGimmu:56698.
UCSCiuc008eyq.2. mouse.

Organism-specific databases

CTDi51808.
MGIiMGI:1891839. Phax.

Phylogenomic databases

eggNOGiKOG3948. Eukaryota.
ENOG4110D9C. LUCA.
GeneTreeiENSGT00390000011084.
HOGENOMiHOG000115525.
HOVERGENiHBG058976.
InParanoidiQ9JJT9.
KOiK14291.
OMAiSQEHTKE.
OrthoDBiEOG091G0XKP.
PhylomeDBiQ9JJT9.
TreeFamiTF321050.

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

PROiPR:Q9JJT9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008301.
ExpressionAtlasiQ9JJT9. baseline and differential.
GenevisibleiQ9JJT9. MM.

Family and domain databases

InterProiView protein in InterPro
IPR019385. PHAX_RNA-binding_domain.
PfamiView protein in Pfam
PF10258. RNA_GG_bind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPHAX_MOUSE
AccessioniPrimary (citable) accession number: Q9JJT9
Secondary accession number(s): Q8BSR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: October 1, 2000
Last modified: June 7, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.