ID   P2RY4_MOUSE             Reviewed;         361 AA.
AC   Q9JJS7;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 159.
DE   RecName: Full=P2Y purinoceptor 4;
DE            Short=P2Y4;
GN   Name=P2ry4; Synonyms=P2y4r;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=129/SvJ;
RX   PubMed=11290369; DOI=10.1016/s0014-2999(01)00875-5;
RA   Suarez-Huerta N., Pouillon V., Boeynaems J.-M., Robaye B.;
RT   "Molecular cloning and characterization of the mouse P2Y4 nucleotide
RT   receptor.";
RL   Eur. J. Pharmacol. 416:197-202(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Skin;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
CC   -!- FUNCTION: Receptor for ATP and UTP coupled to G-proteins that activate
CC       a phosphatidylinositol-calcium second messenger system.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in the liver, intestine, stomach, bladder
CC       and lung.
CC   -!- PTM: Phosphorylation of Ser-329 and Ser-330 is a key step in agonist-
CC       dependent desensitization and loss of surface P2RY4. This
CC       phosphorylation does not involve PKC, nor other calcium-activated
CC       kinases (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; AJ277752; CAB91043.1; -; Genomic_DNA.
DR   EMBL; AK076364; BAC36314.1; -; mRNA.
DR   CCDS; CCDS30303.1; -.
DR   RefSeq; NP_065646.1; NM_020621.4.
DR   RefSeq; XP_006528238.1; XM_006528175.2.
DR   RefSeq; XP_006528239.1; XM_006528176.3.
DR   RefSeq; XP_006528240.1; XM_006528177.2.
DR   RefSeq; XP_006528241.1; XM_006528178.2.
DR   AlphaFoldDB; Q9JJS7; -.
DR   SMR; Q9JJS7; -.
DR   STRING; 10090.ENSMUSP00000055869; -.
DR   GlyCosmos; Q9JJS7; 1 site, No reported glycans.
DR   GlyGen; Q9JJS7; 1 site.
DR   PhosphoSitePlus; Q9JJS7; -.
DR   PaxDb; 10090-ENSMUSP00000055869; -.
DR   ProteomicsDB; 294418; -.
DR   Antibodypedia; 13260; 312 antibodies from 28 providers.
DR   DNASU; 57385; -.
DR   Ensembl; ENSMUST00000053373.2; ENSMUSP00000055869.2; ENSMUSG00000044359.2.
DR   GeneID; 57385; -.
DR   KEGG; mmu:57385; -.
DR   UCSC; uc009twc.1; mouse.
DR   AGR; MGI:1926594; -.
DR   CTD; 5030; -.
DR   MGI; MGI:1926594; P2ry4.
DR   VEuPathDB; HostDB:ENSMUSG00000044359; -.
DR   eggNOG; ENOG502QSTF; Eukaryota.
DR   GeneTree; ENSGT01030000234621; -.
DR   HOGENOM; CLU_009579_8_2_1; -.
DR   InParanoid; Q9JJS7; -.
DR   OMA; TRTIYYM; -.
DR   OrthoDB; 4017151at2759; -.
DR   PhylomeDB; Q9JJS7; -.
DR   TreeFam; TF350009; -.
DR   Reactome; R-MMU-417957; P2Y receptors.
DR   Reactome; R-MMU-418594; G alpha (i) signalling events.
DR   BioGRID-ORCS; 57385; 2 hits in 77 CRISPR screens.
DR   PRO; PR:Q9JJS7; -.
DR   Proteomes; UP000000589; Chromosome X.
DR   RNAct; Q9JJS7; Protein.
DR   Bgee; ENSMUSG00000044359; Expressed in esophagus and 22 other cell types or tissues.
DR   ExpressionAtlas; Q9JJS7; baseline and differential.
DR   GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR   GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0048787; C:presynaptic active zone membrane; ISO:MGI.
DR   GO; GO:0005524; F:ATP binding; ISO:MGI.
DR   GO; GO:0045028; F:G protein-coupled purinergic nucleotide receptor activity; IEA:InterPro.
DR   GO; GO:0045030; F:G protein-coupled UTP receptor activity; IDA:MGI.
DR   GO; GO:0019103; F:pyrimidine nucleotide binding; IC:MGI.
DR   GO; GO:0071380; P:cellular response to prostaglandin E stimulus; IDA:MGI.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0099509; P:regulation of presynaptic cytosolic calcium ion concentration; ISO:MGI.
DR   GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; ISO:MGI.
DR   GO; GO:0030321; P:transepithelial chloride transport; IDA:MGI.
DR   CDD; cd15374; 7tmA_P2Y4; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR000018; P2Y4.
DR   PANTHER; PTHR24231:SF21; P2Y PURINOCEPTOR 4; 1.
DR   PANTHER; PTHR24231; PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   PRINTS; PR01066; P2Y4PRNOCPTR.
DR   PRINTS; PR01157; P2YPURNOCPTR.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; Q9JJS7; MM.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..361
FT                   /note="P2Y purinoceptor 4"
FT                   /id="PRO_0000070022"
FT   TOPO_DOM        1..30
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..58
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        59..68
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..91
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..108
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..127
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        128..149
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        171..192
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        193..218
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        219..242
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..265
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        266..283
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..305
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        306..361
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        175
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        104..181
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ   SEQUENCE   361 AA;  41034 MW;  3E8EA84B65BC0A20 CRC64;
     MTSADSLLFT SLGPSPSSGD GDCKFNEEFK FILLPLSYAV VFVLGLALNA PTLWLFLFRL
     RPWDATATYM FHLALSDTLY VLSLPTLVYY YAARNHWPFG TGFCKFVRFL FYWNLYCSVL
     FLTCISVHRY MGICHPLRAI RWGRPRFAGL LCLGVWLVVA GCLVPNLFFV TTNANGTTIL
     CHDTTLPEEF DHYVYFSSTI MVLLFGFPFL ITLVCYGLMA RRLYRPLPGA GQSSSRLRSL
     RTIAVVLTVF AVCFVPFHIT RTIYYLARLL NAECRVLNIV NVVYKVTRPL ASANSCLDPV
     LYLFTGDKYR NQLQQLCRGS TPKRRTTASS LALVTLHEES ISRWADIHQD SIFPAYEGDR
     L
//