Q9JJS7 (P2RY4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2Y purinoceptor 4
Short name=P2Y4

Gene names

Name:	P2ry4
Synonyms:	P2y4r

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in the liver, intestine, stomach, bladder and lung.
Post-translational modification	Phosphorylation of Ser-329 and Ser-330 is a key step in agonist-dependent desensitization and loss of surface P2RY4. This phosphorylation does not involve PKC, nor other calcium-activated kinases By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transepithelial chloride transport Inferred from direct assay PubMed 11259526. Source: MGI
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: Compara G-protein coupled purinergic nucleotide receptor activity Inferred from electronic annotation. Source: InterPro UTP-activated nucleotide receptor activity Inferred from direct assay PubMed 11259526. Source: MGI pyrimidine nucleotide binding Inferred by curator PubMed 11259526. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	P2Y purinoceptor 4		PRO_0000070022
Regions
	Topological domain	1 – 30	30	Extracellular Potential
	Transmembrane	31 – 58	28	Helical; Name=1; Potential
	Topological domain	59 – 68	10	Cytoplasmic Potential
	Transmembrane	69 – 91	23	Helical; Name=2; Potential
	Topological domain	92 – 108	17	Extracellular Potential
	Transmembrane	109 – 127	19	Helical; Name=3; Potential
	Topological domain	128 – 149	22	Cytoplasmic Potential
	Transmembrane	150 – 170	21	Helical; Name=4; Potential
	Topological domain	171 – 192	22	Extracellular Potential
	Transmembrane	193 – 218	26	Helical; Name=5; Potential
	Topological domain	219 – 242	24	Cytoplasmic Potential
	Transmembrane	243 – 265	23	Helical; Name=6; Potential
	Topological domain	266 – 283	18	Extracellular Potential
	Transmembrane	284 – 305	22	Helical; Name=7; Potential
	Topological domain	306 – 361	56	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 181		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JJS7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3E8EA84B65BC0A20	FASTA	361	41,034
	10 20 30 40 50 60 MTSADSLLFT SLGPSPSSGD GDCKFNEEFK FILLPLSYAV VFVLGLALNA PTLWLFLFRL 70 80 90 100 110 120 RPWDATATYM FHLALSDTLY VLSLPTLVYY YAARNHWPFG TGFCKFVRFL FYWNLYCSVL 130 140 150 160 170 180 FLTCISVHRY MGICHPLRAI RWGRPRFAGL LCLGVWLVVA GCLVPNLFFV TTNANGTTIL 190 200 210 220 230 240 CHDTTLPEEF DHYVYFSSTI MVLLFGFPFL ITLVCYGLMA RRLYRPLPGA GQSSSRLRSL 250 260 270 280 290 300 RTIAVVLTVF AVCFVPFHIT RTIYYLARLL NAECRVLNIV NVVYKVTRPL ASANSCLDPV 310 320 330 340 350 360 LYLFTGDKYR NQLQQLCRGS TPKRRTTASS LALVTLHEES ISRWADIHQD SIFPAYEGDR L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the mouse P2Y4 nucleotide receptor." Suarez-Huerta N., Pouillon V., Boeynaems J.-M., Robaye B. Eur. J. Pharmacol. 416:197-202(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/SvJ.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ277752 Genomic DNA. Translation: CAB91043.1. AK076364 mRNA. Translation: BAC36314.1.
IPI	IPI00122200.
RefSeq	NP_065646.1. NM_020621.4.
UniGene	Mm.117118.
3D structure databases
ProteinModelPortal	Q9JJS7.
SMR	Q9JJS7. Positions 30-311.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9JJS7.
Proteomic databases
PRIDE	Q9JJS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053373; ENSMUSP00000055869; ENSMUSG00000044359.
GeneID	57385.
KEGG	mmu:57385.
Organism-specific databases
CTD	5030.
MGI	MGI:1926594. P2ry4.
Phylogenomic databases
eggNOG	NOG270952.
GeneTree	ENSGT00700000104024.
HOGENOM	HOG000231307.
HOVERGEN	HBG101120.
InParanoid	Q9JJS7.
KO	K04271.
OrthoDB	EOG44J2JK.
Gene expression databases
ArrayExpress	Q9JJS7.
Bgee	Q9JJS7.
CleanEx	MM_P2RY4.
Genevestigator	Q9JJS7.
GermOnline	ENSMUSG00000044359. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR002286. P2_purnocptor. IPR000018. P2Y4_purnocptor. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01066. P2Y4PRNOCPTR. PR01157. P2YPURNOCPTR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	313762.
SOURCE	Search...

Entry information

Entry name

P2RY4_MOUSE

Accession

Primary (citable) accession number: Q9JJS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program