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Protein

P2Y purinoceptor 4

Gene

P2ry4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  1. ATP binding Source: Ensembl
  2. G-protein coupled purinergic nucleotide receptor activity Source: InterPro
  3. pyrimidine nucleotide binding Source: MGI
  4. UTP-activated nucleotide receptor activity Source: MGI

GO - Biological processi

  1. cellular response to prostaglandin E stimulus Source: MGI
  2. transepithelial chloride transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_326987. P2Y receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
P2Y purinoceptor 4
Short name:
P2Y4
Gene namesi
Name:P2ry4
Synonyms:P2y4r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1926594. P2ry4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030ExtracellularSequence AnalysisAdd
BLAST
Transmembranei31 – 5828Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini59 – 6810CytoplasmicSequence Analysis
Transmembranei69 – 9123Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini92 – 10817ExtracellularSequence AnalysisAdd
BLAST
Transmembranei109 – 12719Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini128 – 14922CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei150 – 17021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini171 – 19222ExtracellularSequence AnalysisAdd
BLAST
Transmembranei193 – 21826Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini219 – 24224CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei243 – 26523Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini266 – 28318ExtracellularSequence AnalysisAdd
BLAST
Transmembranei284 – 30522Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini306 – 36156CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. basolateral plasma membrane Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361P2Y purinoceptor 4PRO_0000070022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 181PROSITE-ProRule annotation
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylation of Ser-329 and Ser-330 is a key step in agonist-dependent desensitization and loss of surface P2RY4. This phosphorylation does not involve PKC, nor other calcium-activated kinases (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ9JJS7.

PTM databases

PhosphoSiteiQ9JJS7.

Expressioni

Tissue specificityi

Expressed in the liver, intestine, stomach, bladder and lung.

Gene expression databases

BgeeiQ9JJS7.
CleanExiMM_P2RY4.
ExpressionAtlasiQ9JJS7. baseline and differential.
GenevestigatoriQ9JJS7.

Structurei

3D structure databases

ProteinModelPortaliQ9JJS7.
SMRiQ9JJS7. Positions 30-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG270952.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000231307.
HOVERGENiHBG101120.
InParanoidiQ9JJS7.
KOiK04271.
OMAiYMFHLAL.
OrthoDBiEOG7QNVM4.
PhylomeDBiQ9JJS7.
TreeFamiTF350009.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000018. P2Y4_rcpt.
[Graphical view]
PANTHERiPTHR24231:SF21. PTHR24231:SF21. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01066. P2Y4PRNOCPTR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSADSLLFT SLGPSPSSGD GDCKFNEEFK FILLPLSYAV VFVLGLALNA
60 70 80 90 100
PTLWLFLFRL RPWDATATYM FHLALSDTLY VLSLPTLVYY YAARNHWPFG
110 120 130 140 150
TGFCKFVRFL FYWNLYCSVL FLTCISVHRY MGICHPLRAI RWGRPRFAGL
160 170 180 190 200
LCLGVWLVVA GCLVPNLFFV TTNANGTTIL CHDTTLPEEF DHYVYFSSTI
210 220 230 240 250
MVLLFGFPFL ITLVCYGLMA RRLYRPLPGA GQSSSRLRSL RTIAVVLTVF
260 270 280 290 300
AVCFVPFHIT RTIYYLARLL NAECRVLNIV NVVYKVTRPL ASANSCLDPV
310 320 330 340 350
LYLFTGDKYR NQLQQLCRGS TPKRRTTASS LALVTLHEES ISRWADIHQD
360
SIFPAYEGDR L
Length:361
Mass (Da):41,034
Last modified:October 1, 2000 - v1
Checksum:i3E8EA84B65BC0A20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277752 Genomic DNA. Translation: CAB91043.1.
AK076364 mRNA. Translation: BAC36314.1.
CCDSiCCDS30303.1.
RefSeqiNP_065646.1. NM_020621.4.
XP_006528238.1. XM_006528175.2.
XP_006528239.1. XM_006528176.2.
XP_006528240.1. XM_006528177.2.
XP_006528241.1. XM_006528178.2.
UniGeneiMm.117118.

Genome annotation databases

EnsembliENSMUST00000053373; ENSMUSP00000055869; ENSMUSG00000044359.
GeneIDi57385.
KEGGimmu:57385.
UCSCiuc009twc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277752 Genomic DNA. Translation: CAB91043.1.
AK076364 mRNA. Translation: BAC36314.1.
CCDSiCCDS30303.1.
RefSeqiNP_065646.1. NM_020621.4.
XP_006528238.1. XM_006528175.2.
XP_006528239.1. XM_006528176.2.
XP_006528240.1. XM_006528177.2.
XP_006528241.1. XM_006528178.2.
UniGeneiMm.117118.

3D structure databases

ProteinModelPortaliQ9JJS7.
SMRiQ9JJS7. Positions 30-313.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi325.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9JJS7.

Proteomic databases

PRIDEiQ9JJS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053373; ENSMUSP00000055869; ENSMUSG00000044359.
GeneIDi57385.
KEGGimmu:57385.
UCSCiuc009twc.1. mouse.

Organism-specific databases

CTDi5030.
MGIiMGI:1926594. P2ry4.

Phylogenomic databases

eggNOGiNOG270952.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000231307.
HOVERGENiHBG101120.
InParanoidiQ9JJS7.
KOiK04271.
OMAiYMFHLAL.
OrthoDBiEOG7QNVM4.
PhylomeDBiQ9JJS7.
TreeFamiTF350009.

Enzyme and pathway databases

ReactomeiREACT_326987. P2Y receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi313762.
PROiQ9JJS7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJS7.
CleanExiMM_P2RY4.
ExpressionAtlasiQ9JJS7. baseline and differential.
GenevestigatoriQ9JJS7.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000018. P2Y4_rcpt.
[Graphical view]
PANTHERiPTHR24231:SF21. PTHR24231:SF21. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01066. P2Y4PRNOCPTR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the mouse P2Y4 nucleotide receptor."
    Suarez-Huerta N., Pouillon V., Boeynaems J.-M., Robaye B.
    Eur. J. Pharmacol. 416:197-202(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/SvJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.

Entry informationi

Entry nameiP2RY4_MOUSE
AccessioniPrimary (citable) accession number: Q9JJS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.