Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SH3 domain-binding protein 4

Gene

Sh3bp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 domain-binding protein 4
Gene namesi
Name:Sh3bp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620219. Sh3bp4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961SH3 domain-binding protein 4PRO_0000274576Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei131 – 1311PhosphoserineCombined sources
Modified residuei244 – 2441PhosphoserineCombined sources
Modified residuei249 – 2491PhosphoserineBy similarity
Modified residuei277 – 2771PhosphoserineBy similarity
Modified residuei294 – 2941PhosphoserineBy similarity
Modified residuei635 – 6351PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JJS5.
PRIDEiQ9JJS5.

PTM databases

iPTMnetiQ9JJS5.
PhosphoSiteiQ9JJS5.

Interactioni

Subunit structurei

Homodimer or homooligomer. Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC. Interacts with the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; the interaction is most probably direct, preferentially occurs with their inactive GDP-bound form and is negatively regulated by amino acids (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026312.

Structurei

3D structure databases

ProteinModelPortaliQ9JJS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 11460SH3PROSITE-ProRule annotationAdd
BLAST

Domaini

The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity. The SH3 domain also mediates interaction with RRAGB, RRAGC and is required for the negative regulation of mTORC1 (By similarity).By similarity

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IEE9. Eukaryota.
ENOG41118AI. LUCA.
HOGENOMiHOG000015297.
HOVERGENiHBG057439.
InParanoidiQ9JJS5.
PhylomeDBiQ9JJS5.

Family and domain databases

InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
IPR000906. ZU5_dom.
[Graphical view]
PfamiPF07653. SH3_2. 1 hit.
PF14604. SH3_9. 1 hit.
PF00791. ZU5. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQRIRAAN SSGLPRCKSE GTLIDLSEGF SETSFNDVKV PSPSALLVDN
60 70 80 90 100
PTPFGNAKEV IAIKDYCPNN FTTLKFSKGD HLYVLDTSGG EWWYAHNTTE
110 120 130 140 150
MGYIPSSYVQ PLNYRNSTLS DSGMIDNLPD SPDEVAKELD LLGGWTDDQK
160 170 180 190 200
QSGRPYSNNP FWNGVRTNPF LNGNAQPSMD ELNPKSTVDL LLFDTGTSSF
210 220 230 240 250
TESSSATTNS TGNIFDELPA TNGIHLEQPV KRDNPFFRSK RSYSLSELSV
260 270 280 290 300
LQAKSDAPPT SSFFTGLKSP APEQFQSRED FRTAWLNHRK LARSCHDLDL
310 320 330 340 350
LGQSPGWGQT QAVETNIVCK LDSSGGSVQL PDTSISIHVP EGHVAPGETQ
360 370 380 390 400
QISMKALLDP PLDLNSDRST SISPVVEVKL SNLEVSTFII LEMKVSAEVK
410 420 430 440 450
GDIFSKSTVV LQCLRSDSKE GPYAPIPLAY SYGDTIQVQL DNLEPCMYLA
460 470 480 490 500
IVAQGSNILY PSTVWDFIHK RVTVGLYGPK HIHPSFKTVV TIFGHDCAPK
510 520 530 540 550
TLLVTEVTRQ APSPAPVALQ LWGKHQFILS RPQDLRVCMF SNMTNYDVKS
560 570 580 590 600
NEQAKVVRGF QMKLGKVSRL IFSVISQNPN ELSDFTLRVQ VKDDQDTILT
610 620 630 640 650
QFCVQTPQPP PKSAIKPSGQ RRFLKKNEVG KIILSPFVVT TKYPTFQDRP
660 670 680 690 700
VSSLKFGKLL KTVVRQNKSH YLLEYKKGDV VALLSEERIR LKGQLWTKEW
710 720 730 740 750
YIGYYQGKVG LVHTKNVLVV GKARPSLFSG PELSTSVLLE QILRPCKFLT
760 770 780 790 800
YIYASVRTLL MENISSWRAF ADALGYGNLP LTFFCRAELD SEPERVASVL
810 820 830 840 850
EKLKEDCNNP DNKDRKSFQK ELVMALLKMD CQGLVVRLIQ DFVLLTTAVE
860 870 880 890 900
VAQRWRELAE KLAKVSKQQM DAYESPHRDR NGVVDSEAMW KPAYDFLLTW
910 920 930 940 950
SHQIGDSYRD VIQELHIGLD KMKNPITRRW KHLTGTLILV NSLDILRAAA
960
FSPADHDDFV I
Length:961
Mass (Da):107,513
Last modified:March 1, 2004 - v2
Checksum:iF31DA2AD30B3F35A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278266 mRNA. Translation: CAB93353.2.
UniGeneiRn.35396.

Genome annotation databases

UCSCiRGD:620219. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278266 mRNA. Translation: CAB93353.2.
UniGeneiRn.35396.

3D structure databases

ProteinModelPortaliQ9JJS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026312.

PTM databases

iPTMnetiQ9JJS5.
PhosphoSiteiQ9JJS5.

Proteomic databases

PaxDbiQ9JJS5.
PRIDEiQ9JJS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620219. rat.

Organism-specific databases

RGDi620219. Sh3bp4.

Phylogenomic databases

eggNOGiENOG410IEE9. Eukaryota.
ENOG41118AI. LUCA.
HOGENOMiHOG000015297.
HOVERGENiHBG057439.
InParanoidiQ9JJS5.
PhylomeDBiQ9JJS5.

Miscellaneous databases

PROiQ9JJS5.

Family and domain databases

InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
IPR000906. ZU5_dom.
[Graphical view]
PfamiPF07653. SH3_2. 1 hit.
PF14604. SH3_9. 1 hit.
PF00791. ZU5. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSH3B4_RAT
AccessioniPrimary (citable) accession number: Q9JJS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.