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Protein

Carboxypeptidase N catalytic chain

Gene

Cpn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.By similarity

Catalytic activityi

Release of a C-terminal basic amino acid, preferentially lysine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Zinc; catalyticBy similarity
Metal bindingi89 – 891Zinc; catalyticBy similarity
Metal bindingi216 – 2161Zinc; catalyticBy similarity
Active sitei308 – 3081Proton donor/acceptorBy similarity

GO - Molecular functioni

  • carboxypeptidase activity Source: MGI
  • metallocarboxypeptidase activity Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.3. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase N catalytic chain (EC:3.4.17.3)
Short name:
CPN
Alternative name(s):
Carboxypeptidase N polypeptide 1
Carboxypeptidase N small subunit
Gene namesi
Name:Cpn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2135874. Cpn1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 457434Carboxypeptidase N catalytic chainPRO_0000042578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 104By similarity
Disulfide bondi271 ↔ 311By similarity
Glycosylationi400 – 4001O-linked (GalNAc...)By similarity
Glycosylationi402 – 4021O-linked (GalNAc...)By similarity
Glycosylationi409 – 4091O-linked (GalNAc...)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9JJN5.
MaxQBiQ9JJN5.
PaxDbiQ9JJN5.
PRIDEiQ9JJN5.

PTM databases

PhosphoSiteiQ9JJN5.

Expressioni

Tissue specificityi

Mainly expressed in liver. Also detected in lung, stomach, intestine, spleen and kidney.2 Publications

Gene expression databases

BgeeiQ9JJN5.
CleanExiMM_CPN1.
GenevisibleiQ9JJN5. MM.

Interactioni

Subunit structurei

Tetramer of two catalytic chains and two glycosylated inactive chains.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026210.

Structurei

3D structure databases

ProteinModelPortaliQ9JJN5.
SMRiQ9JJN5. Positions 21-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 340320CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ9JJN5.
KOiK01292.
OMAiDTRIHIM.
OrthoDBiEOG7B8S32.
PhylomeDBiQ9JJN5.
TreeFamiTF315592.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR027063. CPN1.
IPR000834. Peptidase_M14.
[Graphical view]
PANTHERiPTHR11532:SF7. PTHR11532:SF7. 1 hit.
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JJN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDLPSAFLP LLLLSKFVTP VTFRHHRYDD LVRTLYKVHN QCPDITRLYN
60 70 80 90 100
IGRSVKGRYL YVLEFSDYPG IHEPLEPEVK YVGNMHGNEV LGRELLLQLS
110 120 130 140 150
EFLCEEFRNR NQRILRLIQD TRIHILPSMN PDGYEVAAAQ GPNMSGYLVG
160 170 180 190 200
RNNANGVDLN RNFPDLNTYF YYNSKNGGPN HHLPLPDNWK SQVEPETRAV
210 220 230 240 250
IQWIRSLNFV LSANMHGGAV VANYPYDKSL EHRFRGPHRT SNSPTPDDEL
260 270 280 290 300
FQTLAKVYSY AHGWMHQGWN CGDYFPDGIT NGASWYSLSK GMQDFNYLHT
310 320 330 340 350
NCFEITLELS CDKFPRQEEL QREWLGNREA LIQFLEQVHQ GIKGMVLDEN
360 370 380 390 400
SNNLTGAVIS VTGINHDVTS GEHGDYFRLL LPGTYSVTAK APGYDPKTVT
410 420 430 440 450
VTVGPAGPTV VDFQLKRSSS QVYPVQRAPG RGQGGRAKQP RTSRKKDPAT

KRHRGPA
Length:457
Mass (Da):51,845
Last modified:October 1, 2000 - v1
Checksum:iE36FEDA7B44FBC8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti381 – 3811L → LL in AAH14692 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021969 mRNA. Translation: BAB03403.1.
AF326477 mRNA. Translation: AAK06821.1.
BC014692 mRNA. Translation: AAH14692.1.
CCDSiCCDS29840.1.
RefSeqiNP_109628.1. NM_030703.2.
UniGeneiMm.206774.

Genome annotation databases

EnsembliENSMUST00000026210; ENSMUSP00000026210; ENSMUSG00000025196.
GeneIDi93721.
KEGGimmu:93721.
UCSCiuc008hpa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021969 mRNA. Translation: BAB03403.1.
AF326477 mRNA. Translation: AAK06821.1.
BC014692 mRNA. Translation: AAH14692.1.
CCDSiCCDS29840.1.
RefSeqiNP_109628.1. NM_030703.2.
UniGeneiMm.206774.

3D structure databases

ProteinModelPortaliQ9JJN5.
SMRiQ9JJN5. Positions 21-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026210.

PTM databases

PhosphoSiteiQ9JJN5.

Proteomic databases

EPDiQ9JJN5.
MaxQBiQ9JJN5.
PaxDbiQ9JJN5.
PRIDEiQ9JJN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026210; ENSMUSP00000026210; ENSMUSG00000025196.
GeneIDi93721.
KEGGimmu:93721.
UCSCiuc008hpa.1. mouse.

Organism-specific databases

CTDi1369.
MGIiMGI:2135874. Cpn1.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ9JJN5.
KOiK01292.
OMAiDTRIHIM.
OrthoDBiEOG7B8S32.
PhylomeDBiQ9JJN5.
TreeFamiTF315592.

Enzyme and pathway databases

BRENDAi3.4.17.3. 3474.

Miscellaneous databases

PROiQ9JJN5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJN5.
CleanExiMM_CPN1.
GenevisibleiQ9JJN5. MM.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR027063. CPN1.
IPR000834. Peptidase_M14.
[Graphical view]
PANTHERiPTHR11532:SF7. PTHR11532:SF7. 1 hit.
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pro-carboxypeptidase R is an acute phase protein in the mouse, whereas carboxypeptidase N is not."
    Sato T., Miwa T., Akatsu H., Matsukawa N., Obata K., Okada N., Campbell W., Okada H.
    J. Immunol. 165:1053-1058(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Liver.
  2. "Characterization of mouse carboxypeptidase N small active subunit gene structure."
    Matthews K.W., Wetsel R.A.
    J. Immunol. 166:6196-6202(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Liver and Lung.

Entry informationi

Entry nameiCBPN_MOUSE
AccessioniPrimary (citable) accession number: Q9JJN5
Secondary accession number(s): Q91WM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.