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Q9JJN4 (SCO2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial
EC=2.8.3.5
Alternative name(s):
3-oxoacid CoA-transferase 2A
Testis-specific succinyl-CoA:3-oxoacid CoA-transferase 1
Short name=SCOT-t1
Gene names
Name:Oxct2a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate By similarity. Probably play and important roles in the energy metabolism of spermatozoa.

Catalytic activity

Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA.

Pathway

Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the 3-oxoacid CoA-transferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion By similarity
Chain40 – 520481Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial
PRO_0000366209

Sites

Active site3421Acyl-thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JJN4 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: 16BAE0F546AFB993

FASTA52056,473
        10         20         30         40         50         60 
MAALRLLAWA LPRGVSALRP PPALPHRLIR RYVSDRSGSV HFYTDPVKAV EGVKDGSTVM 

        70         80         90        100        110        120 
LGGFGLCGIP ENLIGALKTK GVKDLKIVSS NVGVDDFGLG ILLASKQVRR VVCSYLGENA 

       130        140        150        160        170        180 
LCEKLYLAGE LELEMTPQGT LAERIRAGGT GVPAFYTPTG YGTLVQEGGS PIRYAPDGHL 

       190        200        210        220        230        240 
ITLSEPREVR EFQGRFYLLE HAIRADFALI KGWKADRSGN VIFRGSARNF NVPMCKAADI 

       250        260        270        280        290        300 
SVVEVEEIVD VGTFAPEDIH VPNIYVDRVI KGPKFEKRIE RLTTRDSKPA PGSKDNDPSR 

       310        320        330        340        350        360 
TRIIKRAALE FQDGMYANLG IGIPVLASNY ISPKMTVYLH SENGILGLGP FPLKNEVDAD 

       370        380        390        400        410        420 
VINAGKQTVT VVPGGCFFAS DDSFAMIRGG HLQLTMLGAM QVSQYGDLAN WMVPGKKVKG 

       430        440        450        460        470        480 
MGGAMDLVSS KKTRVVVTME HCTKTKQPKI LKKCTMPLTG KRCVDLIITE KAVFEVNHSK 

       490        500        510        520 
GLTLVELWEG SSVDDIKATT ACSFAVSPNL KPMQQIKLDA

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of haploid germ cell-specific succinyl CoA:3-oxo acid CoA transferase (scot-t1 and scot-t2)."
Tanaka H., Koga M., Yomogida K., Iguchi N., Nozaki M., Onishi M., Egydio de Carvalho C., Nakamura Y., Miyagawa Y., Takeyama M., Matsumiya K., Okuyama A., Nishimune Y.
(In) Robaire B., Chemes H., Morales C.R. (eds.); Andrology in the 21th Century. Proceeding of the VIIth International Congress of Andrology, pp.157-161, Medimond Press, Montreal (2001)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Testis.
[2]"Gene structure and evolution of testicular haploid germ cell-specific genes, Oxct2a and Oxct2b."
Onishi M., Yasunaga T., Tanaka H., Nishimune Y., Nozaki M.
Genomics 83:647-657(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB022180 mRNA. Translation: BAA97654.2.
AB105454 Genomic DNA. Translation: BAC87737.1.
AK077075 mRNA. Translation: BAC36595.1.
AL606917 Genomic DNA. Translation: CAM46094.1.
BC137887 mRNA. Translation: AAI37888.1.
BC137888 mRNA. Translation: AAI37889.1.
IPIIPI00387281.
RefSeqNP_071316.1. NM_022033.4.
XP_003946196.1. XM_003946147.1.
UniGeneMm.270287.

3D structure databases

HSSPHSSP built from PDB template 1OPE based on UniProtKB Q29551.
ProteinModelPortalQ9JJN4.
SMRQ9JJN4. Positions 42-516.
ModBaseSearch...

Proteomic databases

PaxDbQ9JJN4.
PRIDEQ9JJN4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102640; ENSMUSP00000099700; ENSMUSG00000076436.
GeneID101056567.
64059.
KEGGmmu:101056567.
mmu:64059.
UCSCuc008uph.2. mouse.

Organism-specific databases

CTD64059.
MGIMGI:1891061. Oxct2a.

Phylogenomic databases

eggNOGCOG1788.
GeneTreeENSGT00390000009130.
HOGENOMHOG000221244.
HOVERGENHBG002310.
InParanoidQ9JJN4.
KOK01027.
OMAHVPNIYV.
OrthoDBEOG4S4PG7.

Enzyme and pathway databases

BRENDA2.8.3.5. 3474.
UniPathwayUPA00929; UER00894.

Gene expression databases

BgeeQ9JJN4.
GenevestigatorQ9JJN4.

Family and domain databases

InterProIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERPTHR13707. PTHR13707. 1 hit.
PfamPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFPIRSF000858. SCOT-t. 1 hit.
SMARTSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio319883.
SOURCESearch...

Entry information

Entry nameSCO2A_MOUSE
AccessionPrimary (citable) accession number: Q9JJN4
Secondary accession number(s): B9EHG5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 1, 2001
Last modified: April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents