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Q9JJN0

- POLH_MOUSE

UniProt

Q9JJN0 - POLH_MOUSE

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Protein

DNA polymerase eta

Gene
Polh, Rad30a, Xpv
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci By similarity.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Divalent metal cations. Prefers magnesium, but can also use manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Magnesium By similarity
Metal bindingi115 – 1151Magnesium By similarity

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA-dependent DNA replication Source: GOC
  2. DNA synthesis involved in DNA repair Source: Ensembl
  3. postreplication repair Source: MGI
  4. pyrimidine dimer repair Source: MGI
  5. response to UV-C Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase eta (EC:2.7.7.7)
Alternative name(s):
RAD30 homolog A
Xeroderma pigmentosum variant type protein homolog
Gene namesi
Name:Polh
Synonyms:Rad30a, Xpv
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1891457. Polh.

Subcellular locationi

Nucleus By similarity
Note: Accumulates at replication forks after DNA damage By similarity.

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694DNA polymerase etaPRO_0000173987Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki663 – 663Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki667 – 667Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki675 – 675Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki690 – 690Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Post-translational modificationi

Monoubiquitinated by RCHY1/PIRH2; ubiquitination inhibits the ability of PolH to interact with PCNA and to bypass UV-induced lesions By similarity.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9JJN0.
PRIDEiQ9JJN0.

PTM databases

PhosphoSiteiQ9JJN0.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

Up-regulated in proliferating cultured fibroblasts.1 Publication

Gene expression databases

BgeeiQ9JJN0.
CleanExiMM_POLH.
GenevestigatoriQ9JJN0.

Interactioni

Subunit structurei

Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Interacts with PALB2 and BRCA2; the interactions are direct and are involved in POLH localization at collapsed replication forks and DNA polymerization activity.1 Publication

Protein-protein interaction databases

BioGridi219842. 3 interactions.
IntActiQ9JJN0. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9JJN0.
SMRiQ9JJN0. Positions 1-431, 611-643.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 258250UmuCAdd
BLAST

Domaini

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.

Sequence similaritiesi

Contains 1 umuC domain.

Phylogenomic databases

eggNOGiCOG0389.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000115605.
HOVERGENiHBG053633.
InParanoidiQ9JJN0.
KOiK03509.
OMAiSPPLTML.
OrthoDBiEOG7N63M8.
PhylomeDBiQ9JJN0.
TreeFamiTF103010.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017061. DNA_pol_eta.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036603. DPol_eta. 1 hit.
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJN0-1 [UniParc]FASTAAdd to Basket

« Hide

MAPGQNRVVA LVDMDCFFVQ VEQRQNPHLR NKPCAVVQYK SWKGGGIIAV    50
SYEARAFGVT RNMWADDAKK LCPDLLLAQV RESRGKANLT KYREASVEVM 100
EIMSYFAVIE RASIDEAYID LTSAVQERLQ KLQGQPISAD LLPSTYIEGL 150
PRGPTVEETV QKEAIRKQGL LQWLDSLQSD DPTSPDLRLT VGAMIVEEMR 200
AAIESKTGFQ CSAGISHNKV LAKLACGLNK PNRQTLVSHG SVPQLFSQMP 250
IRKIRSLGGK LGASVIEVLG IEYMGDLTQF TESQLQSHFG EKNGSWLYAM 300
CRGIEHDPVK PRQLPKTIGC SKNFPGKTAL ATREQVQWWL LQLALELEER 350
LTKDRNDNDR VATQLVVSIR FQGDRRLSSL RRCCALPRYD AHKMSQDAFA 400
AIRNCNTSGI QTEWSPPLTM LFLCATKFSA AAPPACTDIT AFLSSDSSCQ 450
PKVPIASSET RTQGSGPAVP TSKEAATSLA SFFQKAAKKQ RMKETSFVPL 500
NTATEKLSSK PSLVFQSSQT TGSQSFFKQK SLLLQHTQLS NSAAPDPPQA 550
SPAAQPSCLP AECVDSGPDD GAVKPVSSKA VSTEMNVAGD SPNVLDSPAY 600
NSQEVTQRAT EDQVLCEKCD SLVPVWDMPE HTDYHFALEL QKSFLQPCTS 650
KPQAIPAVSP QGKRNPKSPS ASSSKRLRPH GMQTLESFFK PLTH 694
Length:694
Mass (Da):76,167
Last modified:October 1, 2000 - v1
Checksum:i727895AB5DB84BC8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB027128 mRNA. Translation: BAA97570.1.
AB037184 mRNA. Translation: BAA97585.1.
AK036296 mRNA. Translation: BAC29375.1.
CCDSiCCDS28822.1.
RefSeqiNP_109640.1. NM_030715.3.
UniGeneiMm.311585.

Genome annotation databases

EnsembliENSMUST00000024749; ENSMUSP00000024749; ENSMUSG00000023953.
GeneIDi80905.
KEGGimmu:80905.
UCSCiuc008crx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB027128 mRNA. Translation: BAA97570.1 .
AB037184 mRNA. Translation: BAA97585.1 .
AK036296 mRNA. Translation: BAC29375.1 .
CCDSi CCDS28822.1.
RefSeqi NP_109640.1. NM_030715.3.
UniGenei Mm.311585.

3D structure databases

ProteinModelPortali Q9JJN0.
SMRi Q9JJN0. Positions 1-431, 611-643.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219842. 3 interactions.
IntActi Q9JJN0. 1 interaction.

PTM databases

PhosphoSitei Q9JJN0.

Proteomic databases

PaxDbi Q9JJN0.
PRIDEi Q9JJN0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024749 ; ENSMUSP00000024749 ; ENSMUSG00000023953 .
GeneIDi 80905.
KEGGi mmu:80905.
UCSCi uc008crx.1. mouse.

Organism-specific databases

CTDi 5429.
MGIi MGI:1891457. Polh.

Phylogenomic databases

eggNOGi COG0389.
GeneTreei ENSGT00530000062942.
HOGENOMi HOG000115605.
HOVERGENi HBG053633.
InParanoidi Q9JJN0.
KOi K03509.
OMAi SPPLTML.
OrthoDBi EOG7N63M8.
PhylomeDBi Q9JJN0.
TreeFami TF103010.

Miscellaneous databases

NextBioi 350254.
PROi Q9JJN0.
SOURCEi Search...

Gene expression databases

Bgeei Q9JJN0.
CleanExi MM_POLH.
Genevestigatori Q9JJN0.

Family and domain databases

Gene3Di 3.30.1490.100. 1 hit.
InterProi IPR017061. DNA_pol_eta.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
[Graphical view ]
Pfami PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF036603. DPol_eta. 1 hit.
SUPFAMi SSF100879. SSF100879. 1 hit.
PROSITEi PS50173. UMUC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complementation of defective translesion synthesis and UV light-sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase eta."
    Yamada A., Masutani C., Iwai S., Hanaoka F.
    Nucleic Acids Res. 28:2473-2480(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    Strain: 129/Sv.
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. "Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis."
    Guo C., Fischhaber P.L., Luk-Paszyc M.J., Masuda Y., Zhou J., Kamiya K., Kisker C., Friedberg E.C.
    EMBO J. 22:6621-6630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH REV1.

Entry informationi

Entry nameiPOLH_MOUSE
AccessioniPrimary (citable) accession number: Q9JJN0
Secondary accession number(s): Q9JJJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi