Q9JJM9 (SEPT5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Septin-5

Alternative name(s):
Cell division control-related protein 1
Short name=CDCrel-1
Peanut-like protein 1

Gene names

Name:	Sept5
Synonyms:	Pnutl1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Filament-forming cytoskeletal GTPase By similarity. May play a role in cytokinesis Potential. May play a role in platelet secretion By similarity.
Subunit structure	Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Interacts with SEPT2 and SEPT5. In platelets, associated with a complex containing STX4. Interacts with PARK2. This interaction leads to SEPT5 ubiquitination and degradation By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity.
Tissue specificity	Expressed in brain and testis and at lower level in heart, spleen, lung and kidney. Ref.1
Sequence similarities	Belongs to the septin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of exocytosis Inferred from mutant phenotype PubMed 10321247. Source: RGD
Cellular_component	cleavage furrow Inferred from direct assay PubMed 17685441. Source: UniProtKB plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB septin complex Inferred from direct assay PubMed 17685441. Source: UniProtKB stress fiber Inferred from direct assay PubMed 17685441. Source: UniProtKB synaptic vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	GDP binding Inferred from direct assay PubMed 17685441. Source: UniProtKB GTP binding Inferred from direct assay PubMed 17685441. Source: UniProtKB phosphatidylinositol binding Inferred from direct assay PubMed 17685441. Source: UniProtKB syntaxin binding Inferred from mutant phenotype PubMed 10321247. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JJM9-1)

Also known as: CDCrel-1F;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JJM9-2)

Also known as: CDCrel-1A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSTGLRYKSKLATPEDKQ → MDSLAAPQDRLVEQLLSPRTQAQRRLK

Isoform 3 (identifier: Q9JJM9-3)

Also known as: CDCrel-1AI;

The sequence of this isoform differs from the canonical sequence as follows:
     352-356: LRRMQ → GRAGR
     357-369: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Septin-5		PRO_0000173524
Regions
	Nucleotide binding	51 – 58	8	GTP By similarity
	Nucleotide binding	190 – 198	9	GTP By similarity
	Coiled coil	338 – 369	32	Potential
Sites
	Binding site	85	1	GTP By similarity
	Binding site	111	1	GTP; via amide nitrogen By similarity
	Binding site	248	1	GTP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	263	1	GTP By similarity
Amino acid modifications
	Modified residue	225	1	Phosphoserine By similarity
	Modified residue	327	1	Phosphoserine By similarity
	Modified residue	336	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 18	18	MSTGL…PEDKQ → MDSLAAPQDRLVEQLLSPRT QAQRRLK in isoform 2.		VSP_016539
	Alternative sequence	352 – 356	5	LRRMQ → GRAGR in isoform 3.		VSP_016540
	Alternative sequence	357 – 369	13	Missing in isoform 3.		VSP_016541

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CDCrel-1F) [UniParc]. Last modified December 6, 2005. Version 2. Checksum: FBE46DDAAB7DFC77	FASTA	369	42,852
	10 20 30 40 50 60 MSTGLRYKSK LATPEDKQDI DKQYVGFATL PNQVHRKSVK KGFDFTLMVA GESGLGKSTL 70 80 90 100 110 120 VHSLFLTDLY KDRKLLSAEE RINQTVEILK HTVDIEEKGV KLKLTIVDTP GFGDAVNNFE 130 140 150 160 170 180 CWKPITDYVD QQFEQYFRDE SGLNRKNIQD NRVHCCLYFI SPFGHGLRPV DVGFMKALHE 190 200 210 220 230 240 KVNIVPLIAK ADCLVPSEIR KLKDRIREEI DKFGIHVYQF PECDSDEDED FKQQDRELKE 250 260 270 280 290 300 SAPFAVIGSN TVVEAKGQRV RGRLYPWGIV EVENQAHCDF VKLRNMLIRT HMHDLKDVTC 310 320 330 340 350 360 DVHYENYRAH CIQQMTSKLT QDSRMESPIP ILPLPTPDSE TEKLIRMKDE ELRRMQEMLQ KMKQRMQDQ
	Isoform 2 (CDCrel-1A) [UniParc]. Checksum: B3341620323B4A49	FASTA	378	43,920
	10 20 30 40 50 60 MDSLAAPQDR LVEQLLSPRT QAQRRLKDID KQYVGFATLP NQVHRKSVKK GFDFTLMVAG 70 80 90 100 110 120 ESGLGKSTLV HSLFLTDLYK DRKLLSAEER INQTVEILKH TVDIEEKGVK LKLTIVDTPG 130 140 150 160 170 180 FGDAVNNFEC WKPITDYVDQ QFEQYFRDES GLNRKNIQDN RVHCCLYFIS PFGHGLRPVD 190 200 210 220 230 240 VGFMKALHEK VNIVPLIAKA DCLVPSEIRK LKDRIREEID KFGIHVYQFP ECDSDEDEDF 250 260 270 280 290 300 KQQDRELKES APFAVIGSNT VVEAKGQRVR GRLYPWGIVE VENQAHCDFV KLRNMLIRTH 310 320 330 340 350 360 MHDLKDVTCD VHYENYRAHC IQQMTSKLTQ DSRMESPIPI LPLPTPDSET EKLIRMKDEE 370 LRRMQEMLQK MKQRMQDQ
	Isoform 3 (CDCrel-1AI) [UniParc]. Checksum: 87B852E69B406761	FASTA	356	40,989
	10 20 30 40 50 60 MSTGLRYKSK LATPEDKQDI DKQYVGFATL PNQVHRKSVK KGFDFTLMVA GESGLGKSTL 70 80 90 100 110 120 VHSLFLTDLY KDRKLLSAEE RINQTVEILK HTVDIEEKGV KLKLTIVDTP GFGDAVNNFE 130 140 150 160 170 180 CWKPITDYVD QQFEQYFRDE SGLNRKNIQD NRVHCCLYFI SPFGHGLRPV DVGFMKALHE 190 200 210 220 230 240 KVNIVPLIAK ADCLVPSEIR KLKDRIREEI DKFGIHVYQF PECDSDEDED FKQQDRELKE 250 260 270 280 290 300 SAPFAVIGSN TVVEAKGQRV RGRLYPWGIV EVENQAHCDF VKLRNMLIRT HMHDLKDVTC 310 320 330 340 350 DVHYENYRAH CIQQMTSKLT QDSRMESPIP ILPLPTPDSE TEKLIRMKDE EGRAGR

References

[1]	"Reciprocal expression of infant- and adult preferring transcripts of CDCrel-1 septin in the rat neocortex." Tada S., Kajii Y., Sato M., Nishikawa T. Biochem. Biophys. Res. Commun. 273:723-728(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY. Strain: Wistar. Tissue: Neonatal brain.
[2]	Lubec G., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-36 AND 297-308, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB027143 mRNA. Translation: BAA98051.1. AB027144 mRNA. Translation: BAB87114.1. AB027145 mRNA. Translation: BAA98052.1.
IPI	IPI00201737. IPI00559449. IPI00655290.
RefSeq	NP_446383.3. NM_053931.3.
UniGene	Rn.96497.
3D structure databases
ProteinModelPortal	Q9JJM9.
SMR	Q9JJM9. Positions 27-310.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JJM9. 2 interactions.
STRING	10116.ENSRNOP00000042895.
PTM databases
PhosphoSite	Q9JJM9.
Proteomic databases
PRIDE	Q9JJM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	116728.
KEGG	rno:116728.
Organism-specific databases
CTD	5413.
RGD	621763. Sept5.
Phylogenomic databases
HOGENOM	HOG000233586.
HOVERGEN	HBG065093.
KO	K04557.
Gene expression databases
ArrayExpress	Q9JJM9.
Genevestigator	Q9JJM9.
GermOnline	ENSRNOG00000029912. Rattus norvegicus.
Family and domain databases
InterPro	IPR000038. Cell_div_GTP-bd. IPR016491. Septin. [Graphical view]
PANTHER	PTHR18884. PTHR18884. 1 hit.
Pfam	PF00735. Septin. 1 hit. [Graphical view]
PIRSF	PIRSF006698. Septin. 1 hit.
ProtoNet	Search...
Other
NextBio	619662.

Entry information

Entry name

SEPT5_RAT

Accession

Primary (citable) accession number: Q9JJM9
Secondary accession number(s): Q8R2F7, Q9JJM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program