Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-serine O-palmitoleoyltransferase porcupine

Gene

Porcn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors.5 Publications

Catalytic activityi

(9Z)-hexadecenoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadecenoyl-L-serine.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei341 – 34111 Publication

GO - Molecular functioni

  • O-acyltransferase activity Source: Reactome
  • palmitoleoyltransferase activity Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • glycoprotein metabolic process Source: MGI
  • protein lipidation Source: UniProtKB
  • protein palmitoleylation Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-serine O-palmitoleoyltransferase porcupineCurated (EC:2.3.1.-2 Publications)
Short name:
mPORC
Gene namesi
Name:PorcnImported
Synonyms:Porc, Ppn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1890212. Porcn.

Subcellular locationi

  • Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717CytoplasmicSequence analysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Topological domaini39 – 6628ExtracellularSequence analysisAdd
BLAST
Transmembranei67 – 8721HelicalSequence analysisAdd
BLAST
Topological domaini88 – 958CytoplasmicSequence analysis
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 15236ExtracellularSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Topological domaini174 – 19825CytoplasmicSequence analysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Topological domaini220 – 25233ExtracellularSequence analysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST
Topological domaini274 – 33764CytoplasmicSequence analysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Topological domaini359 – 39638ExtracellularSequence analysisAdd
BLAST
Transmembranei397 – 41721HelicalSequence analysisAdd
BLAST
Topological domaini418 – 46144CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • AMPA glutamate receptor complex Source: MGI
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of endoplasmic reticulum membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi136 – 1361S → F: Impaired acyltransferase activity. 1 Publication
Mutagenesisi172 – 1721S → A: Does not affect acyltransferase activity. 1 Publication
Mutagenesisi228 – 2281R → C: Does not affect acyltransferase activity. 1 Publication
Mutagenesisi305 – 3051W → A: Impaired acyltransferase activity. 1 Publication
Mutagenesisi306 – 3061N → A: Does not affect acyltransferase activity. 1 Publication
Mutagenesisi312 – 3121W → A: Does not affect acyltransferase activity. 1 Publication
Mutagenesisi316 – 3161Y → A: Impaired acyltransferase activity. 1 Publication
Mutagenesisi331 – 3311L → R: Impaired acyltransferase activity. 1 Publication
Mutagenesisi334 – 3341Y → A: Impaired acyltransferase activity. Impaired ability to interact with WNT3A. 1 Publication
Mutagenesisi337 – 3371S → A: Abolished acyltransferase activity. Impaired ability to interact with WNT3A. 1 Publication
Mutagenesisi340 – 3401L → A: Abolished acyltransferase activity. 1 Publication
Mutagenesisi341 – 3411H → A: Abolished acyltransferase activity. Impaired ability to interact with WNT3A. 1 Publication
Mutagenesisi365 – 3651R → Q: Impaired acyltransferase activity. 1 Publication

Chemistry

ChEMBLiCHEMBL1255164.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Protein-serine O-palmitoleoyltransferase porcupinePRO_0000213138Add
BLAST

Proteomic databases

MaxQBiQ9JJJ7.
PaxDbiQ9JJJ7.
PRIDEiQ9JJJ7.

PTM databases

PhosphoSiteiQ9JJJ7.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, muscle, spleen and testis. Isoform 4 is strongly expressed in kidney, liver, lung, spleen and testis. Isoform 1 is strongly expressed in brain, heart and muscle and poorly in kidney, liver, lung, spleen and testis.1 Publication

Developmental stagei

Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed at different levels in embryo at 9.5, 10.5, 11.5, 12.5, 13.5 and 15.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000031169.
CleanExiMM_PORCN.
ExpressionAtlasiQ9JJJ7. baseline and differential.
GenevisibleiQ9JJJ7. MM.

Interactioni

Subunit structurei

Interacts with WNT1, WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A and WNT7B.2 Publications

GO - Molecular functioni

  • Wnt-protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207344. 9 interactions.
STRINGi10090.ENSMUSP00000076790.

Chemistry

BindingDBiQ9JJJ7.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4312. Eukaryota.
ENOG410YDZ6. LUCA.
GeneTreeiENSGT00440000033687.
HOVERGENiHBG061243.
InParanoidiQ9JJJ7.
KOiK00181.
OMAiELSWTSH.
OrthoDBiEOG091G0DRB.
PhylomeDBiQ9JJJ7.
TreeFamiTF313724.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJJ7-1) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATFSRQEFF QQLLQGCLLP TVQQGLDQIW LLLTICFACR LLWRLGLPSY
60 70 80 90 100
LKHASTVAGG FFSLYHFFQL HMVWVVLLSL LCYLVLFLCR HSSHRGVFLS
110 120 130 140 150
VTILIYLLMG EMHMVDTVTW HKMRGAQMIV AMKAVSLGFD LDRGEVGAVP
160 170 180 190 200
SPVEFMGYLY FVGTIVFGPW ISFHSYLQAV QGRPLSRRWL KKVARSLALA
210 220 230 240 250
LLCLVLSTCV GPYLFPYFIP LDGDRLLRNK KRKARGTMVR WLRAYESAVS
260 270 280 290 300
FHFSNYFVGF LSEATATLAG AGFTEEKDHL EWDLTVSRPL NVELPRSMVE
310 320 330 340 350
VVTSWNLPMS YWLNNYVFKN ALRLGTFSAV LVTYAASALL HGFSFHLAAV
360 370 380 390 400
LLSLAFITYV EHVLRKRLAQ ILSACILSKR CLPDCSHRHR LGLGVRALNL
410 420 430 440 450
LFGALAIFHL SYLGSLFDVD VDDTTEEQGY GMAYTVHKWS ELSWASHWVT
460
FGCWIFYRLI G
Length:461
Mass (Da):52,508
Last modified:October 1, 2000 - v1
Checksum:i25A52681C251A0A6
GO
Isoform 2 (identifier: Q9JJJ7-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     235-239: Missing.

Show »
Length:456
Mass (Da):51,963
Checksum:i2903AD4BD0CA2184
GO
Isoform 3 (identifier: Q9JJJ7-3) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     229-235: NKKRKAR → K

Show »
Length:455
Mass (Da):51,754
Checksum:iA78DCC9613A18319
GO
Isoform 4 (identifier: Q9JJJ7-4) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     229-240: NKKRKARGTMVR → K

Show »
Length:450
Mass (Da):51,209
Checksum:i992012ABE6B649E8
GO
Isoform 5 (identifier: Q9JJJ7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-239: RGTMV → MAQDA

Show »
Length:227
Mass (Da):25,726
Checksum:i4B78150917A1685B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 234234Missing in isoform 5. 1 PublicationVSP_015889Add
BLAST
Alternative sequencei229 – 24012NKKRK…GTMVR → K in isoform 4. 1 PublicationVSP_015890Add
BLAST
Alternative sequencei229 – 2357NKKRKAR → K in isoform 3. 2 PublicationsVSP_015891
Alternative sequencei235 – 2395Missing in isoform 2. 1 PublicationVSP_015892
Alternative sequencei235 – 2395RGTMV → MAQDA in isoform 5. 1 PublicationVSP_015893

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036746 mRNA. Translation: BAA89467.1.
AB036747 mRNA. Translation: BAA89468.1.
AB036748 mRNA. Translation: BAA89469.1.
AB036749 mRNA. Translation: BAA89470.1.
AK010405 mRNA. Translation: BAB26914.1.
AL663032 Genomic DNA. Translation: CAM19505.1.
AL663032 Genomic DNA. Translation: CAM19507.1.
AL663032 Genomic DNA. Translation: CAM19508.1.
BC032284 mRNA. Translation: AAH32284.1.
CCDSiCCDS29990.1. [Q9JJJ7-4]
CCDS29991.1. [Q9JJJ7-1]
CCDS29992.1. [Q9JJJ7-3]
CCDS29993.1. [Q9JJJ7-2]
RefSeqiNP_001295403.1. NM_001308474.1. [Q9JJJ7-5]
NP_058609.1. NM_016913.4. [Q9JJJ7-4]
NP_076127.1. NM_023638.4. [Q9JJJ7-1]
NP_665914.1. NM_145907.4. [Q9JJJ7-3]
NP_665915.1. NM_145908.4. [Q9JJJ7-2]
UniGeneiMm.443425.

Genome annotation databases

EnsembliENSMUST00000077595; ENSMUSP00000076790; ENSMUSG00000031169. [Q9JJJ7-1]
ENSMUST00000082320; ENSMUSP00000080937; ENSMUSG00000031169. [Q9JJJ7-2]
ENSMUST00000089402; ENSMUSP00000086824; ENSMUSG00000031169. [Q9JJJ7-4]
ENSMUST00000089403; ENSMUSP00000086825; ENSMUSG00000031169. [Q9JJJ7-3]
GeneIDi53627.
KEGGimmu:53627.
UCSCiuc009soj.2. mouse. [Q9JJJ7-1]
uc009sok.2. mouse. [Q9JJJ7-2]
uc009sol.2. mouse. [Q9JJJ7-3]
uc009som.2. mouse. [Q9JJJ7-4]
uc012heo.1. mouse. [Q9JJJ7-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036746 mRNA. Translation: BAA89467.1.
AB036747 mRNA. Translation: BAA89468.1.
AB036748 mRNA. Translation: BAA89469.1.
AB036749 mRNA. Translation: BAA89470.1.
AK010405 mRNA. Translation: BAB26914.1.
AL663032 Genomic DNA. Translation: CAM19505.1.
AL663032 Genomic DNA. Translation: CAM19507.1.
AL663032 Genomic DNA. Translation: CAM19508.1.
BC032284 mRNA. Translation: AAH32284.1.
CCDSiCCDS29990.1. [Q9JJJ7-4]
CCDS29991.1. [Q9JJJ7-1]
CCDS29992.1. [Q9JJJ7-3]
CCDS29993.1. [Q9JJJ7-2]
RefSeqiNP_001295403.1. NM_001308474.1. [Q9JJJ7-5]
NP_058609.1. NM_016913.4. [Q9JJJ7-4]
NP_076127.1. NM_023638.4. [Q9JJJ7-1]
NP_665914.1. NM_145907.4. [Q9JJJ7-3]
NP_665915.1. NM_145908.4. [Q9JJJ7-2]
UniGeneiMm.443425.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207344. 9 interactions.
STRINGi10090.ENSMUSP00000076790.

Chemistry

BindingDBiQ9JJJ7.
ChEMBLiCHEMBL1255164.

PTM databases

PhosphoSiteiQ9JJJ7.

Proteomic databases

MaxQBiQ9JJJ7.
PaxDbiQ9JJJ7.
PRIDEiQ9JJJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077595; ENSMUSP00000076790; ENSMUSG00000031169. [Q9JJJ7-1]
ENSMUST00000082320; ENSMUSP00000080937; ENSMUSG00000031169. [Q9JJJ7-2]
ENSMUST00000089402; ENSMUSP00000086824; ENSMUSG00000031169. [Q9JJJ7-4]
ENSMUST00000089403; ENSMUSP00000086825; ENSMUSG00000031169. [Q9JJJ7-3]
GeneIDi53627.
KEGGimmu:53627.
UCSCiuc009soj.2. mouse. [Q9JJJ7-1]
uc009sok.2. mouse. [Q9JJJ7-2]
uc009sol.2. mouse. [Q9JJJ7-3]
uc009som.2. mouse. [Q9JJJ7-4]
uc012heo.1. mouse. [Q9JJJ7-5]

Organism-specific databases

CTDi64840.
MGIiMGI:1890212. Porcn.

Phylogenomic databases

eggNOGiKOG4312. Eukaryota.
ENOG410YDZ6. LUCA.
GeneTreeiENSGT00440000033687.
HOVERGENiHBG061243.
InParanoidiQ9JJJ7.
KOiK00181.
OMAiELSWTSH.
OrthoDBiEOG091G0DRB.
PhylomeDBiQ9JJJ7.
TreeFamiTF313724.

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Miscellaneous databases

PROiQ9JJJ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031169.
CleanExiMM_PORCN.
ExpressionAtlasiQ9JJJ7. baseline and differential.
GenevisibleiQ9JJJ7. MM.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPORCN_MOUSE
AccessioniPrimary (citable) accession number: Q9JJJ7
Secondary accession number(s): A2AC30
, A2AC32, A2AC33, Q9CWT0, Q9JJJ8, Q9JJJ9, Q9JJK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was initially thought to mediate palmitoylation of Wnt proteins. It was later shown that it instead acts as a serine O-palmitoleoyltransferase that mediates the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins (PubMed:17141155, PubMed:24798332).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.