Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-serine O-palmitoleoyltransferase porcupine

Gene

Porcn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors.5 Publications

Catalytic activityi

(9Z)-hexadecenoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadecenoyl-L-serine.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3411 Publication1

GO - Molecular functioni

  • O-acyltransferase activity Source: Reactome
  • palmitoleoyltransferase activity Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • glycoprotein metabolic process Source: MGI
  • protein lipidation Source: UniProtKB
  • protein palmitoleylation Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-serine O-palmitoleoyltransferase porcupineCurated (EC:2.3.1.-2 Publications)
Short name:
mPORC
Gene namesi
Name:PorcnImported
Synonyms:Porc, Ppn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1890212. Porcn.

Subcellular locationi

  • Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicSequence analysisAdd BLAST17
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Topological domaini39 – 66ExtracellularSequence analysisAdd BLAST28
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 95CytoplasmicSequence analysis8
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 152ExtracellularSequence analysisAdd BLAST36
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 198CytoplasmicSequence analysisAdd BLAST25
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Topological domaini220 – 252ExtracellularSequence analysisAdd BLAST33
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21
Topological domaini274 – 337CytoplasmicSequence analysisAdd BLAST64
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359 – 396ExtracellularSequence analysisAdd BLAST38
Transmembranei397 – 417HelicalSequence analysisAdd BLAST21
Topological domaini418 – 461CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

  • AMPA glutamate receptor complex Source: MGI
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of endoplasmic reticulum membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136S → F: Impaired acyltransferase activity. 1 Publication1
Mutagenesisi172S → A: Does not affect acyltransferase activity. 1 Publication1
Mutagenesisi228R → C: Does not affect acyltransferase activity. 1 Publication1
Mutagenesisi305W → A: Impaired acyltransferase activity. 1 Publication1
Mutagenesisi306N → A: Does not affect acyltransferase activity. 1 Publication1
Mutagenesisi312W → A: Does not affect acyltransferase activity. 1 Publication1
Mutagenesisi316Y → A: Impaired acyltransferase activity. 1 Publication1
Mutagenesisi331L → R: Impaired acyltransferase activity. 1 Publication1
Mutagenesisi334Y → A: Impaired acyltransferase activity. Impaired ability to interact with WNT3A. 1 Publication1
Mutagenesisi337S → A: Abolished acyltransferase activity. Impaired ability to interact with WNT3A. 1 Publication1
Mutagenesisi340L → A: Abolished acyltransferase activity. 1 Publication1
Mutagenesisi341H → A: Abolished acyltransferase activity. Impaired ability to interact with WNT3A. 1 Publication1
Mutagenesisi365R → Q: Impaired acyltransferase activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1255164.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002131381 – 461Protein-serine O-palmitoleoyltransferase porcupineAdd BLAST461

Proteomic databases

PaxDbiQ9JJJ7.
PRIDEiQ9JJJ7.

PTM databases

PhosphoSitePlusiQ9JJJ7.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, muscle, spleen and testis. Isoform 4 is strongly expressed in kidney, liver, lung, spleen and testis. Isoform 1 is strongly expressed in brain, heart and muscle and poorly in kidney, liver, lung, spleen and testis.1 Publication

Developmental stagei

Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed at different levels in embryo at 9.5, 10.5, 11.5, 12.5, 13.5 and 15.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000031169.
CleanExiMM_PORCN.
ExpressionAtlasiQ9JJJ7. baseline and differential.
GenevisibleiQ9JJJ7. MM.

Interactioni

Subunit structurei

Interacts with WNT1, WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A and WNT7B.2 Publications

GO - Molecular functioni

  • Wnt-protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207344. 9 interactors.
STRINGi10090.ENSMUSP00000076790.

Chemistry databases

BindingDBiQ9JJJ7.

Structurei

3D structure databases

ProteinModelPortaliQ9JJJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4312. Eukaryota.
ENOG410YDZ6. LUCA.
GeneTreeiENSGT00440000033687.
HOVERGENiHBG061243.
InParanoidiQ9JJJ7.
KOiK00181.
OMAiELSWTSH.
OrthoDBiEOG091G0DRB.
PhylomeDBiQ9JJJ7.
TreeFamiTF313724.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJJ7-1) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATFSRQEFF QQLLQGCLLP TVQQGLDQIW LLLTICFACR LLWRLGLPSY
60 70 80 90 100
LKHASTVAGG FFSLYHFFQL HMVWVVLLSL LCYLVLFLCR HSSHRGVFLS
110 120 130 140 150
VTILIYLLMG EMHMVDTVTW HKMRGAQMIV AMKAVSLGFD LDRGEVGAVP
160 170 180 190 200
SPVEFMGYLY FVGTIVFGPW ISFHSYLQAV QGRPLSRRWL KKVARSLALA
210 220 230 240 250
LLCLVLSTCV GPYLFPYFIP LDGDRLLRNK KRKARGTMVR WLRAYESAVS
260 270 280 290 300
FHFSNYFVGF LSEATATLAG AGFTEEKDHL EWDLTVSRPL NVELPRSMVE
310 320 330 340 350
VVTSWNLPMS YWLNNYVFKN ALRLGTFSAV LVTYAASALL HGFSFHLAAV
360 370 380 390 400
LLSLAFITYV EHVLRKRLAQ ILSACILSKR CLPDCSHRHR LGLGVRALNL
410 420 430 440 450
LFGALAIFHL SYLGSLFDVD VDDTTEEQGY GMAYTVHKWS ELSWASHWVT
460
FGCWIFYRLI G
Length:461
Mass (Da):52,508
Last modified:October 1, 2000 - v1
Checksum:i25A52681C251A0A6
GO
Isoform 2 (identifier: Q9JJJ7-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     235-239: Missing.

Show »
Length:456
Mass (Da):51,963
Checksum:i2903AD4BD0CA2184
GO
Isoform 3 (identifier: Q9JJJ7-3) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     229-235: NKKRKAR → K

Show »
Length:455
Mass (Da):51,754
Checksum:iA78DCC9613A18319
GO
Isoform 4 (identifier: Q9JJJ7-4) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     229-240: NKKRKARGTMVR → K

Show »
Length:450
Mass (Da):51,209
Checksum:i992012ABE6B649E8
GO
Isoform 5 (identifier: Q9JJJ7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-239: RGTMV → MAQDA

Show »
Length:227
Mass (Da):25,726
Checksum:i4B78150917A1685B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0158891 – 234Missing in isoform 5. 1 PublicationAdd BLAST234
Alternative sequenceiVSP_015890229 – 240NKKRK…GTMVR → K in isoform 4. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_015891229 – 235NKKRKAR → K in isoform 3. 2 Publications7
Alternative sequenceiVSP_015892235 – 239Missing in isoform 2. 1 Publication5
Alternative sequenceiVSP_015893235 – 239RGTMV → MAQDA in isoform 5. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036746 mRNA. Translation: BAA89467.1.
AB036747 mRNA. Translation: BAA89468.1.
AB036748 mRNA. Translation: BAA89469.1.
AB036749 mRNA. Translation: BAA89470.1.
AK010405 mRNA. Translation: BAB26914.1.
AL663032 Genomic DNA. Translation: CAM19505.1.
AL663032 Genomic DNA. Translation: CAM19507.1.
AL663032 Genomic DNA. Translation: CAM19508.1.
BC032284 mRNA. Translation: AAH32284.1.
CCDSiCCDS29990.1. [Q9JJJ7-4]
CCDS29991.1. [Q9JJJ7-1]
CCDS29992.1. [Q9JJJ7-3]
CCDS29993.1. [Q9JJJ7-2]
RefSeqiNP_001295403.1. NM_001308474.1. [Q9JJJ7-5]
NP_058609.1. NM_016913.4. [Q9JJJ7-4]
NP_076127.1. NM_023638.4. [Q9JJJ7-1]
NP_665914.1. NM_145907.4. [Q9JJJ7-3]
NP_665915.1. NM_145908.4. [Q9JJJ7-2]
UniGeneiMm.443425.

Genome annotation databases

EnsembliENSMUST00000077595; ENSMUSP00000076790; ENSMUSG00000031169. [Q9JJJ7-1]
ENSMUST00000082320; ENSMUSP00000080937; ENSMUSG00000031169. [Q9JJJ7-2]
ENSMUST00000089402; ENSMUSP00000086824; ENSMUSG00000031169. [Q9JJJ7-4]
ENSMUST00000089403; ENSMUSP00000086825; ENSMUSG00000031169. [Q9JJJ7-3]
GeneIDi53627.
KEGGimmu:53627.
UCSCiuc009soj.2. mouse. [Q9JJJ7-1]
uc009sok.2. mouse. [Q9JJJ7-2]
uc009sol.2. mouse. [Q9JJJ7-3]
uc009som.2. mouse. [Q9JJJ7-4]
uc012heo.1. mouse. [Q9JJJ7-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036746 mRNA. Translation: BAA89467.1.
AB036747 mRNA. Translation: BAA89468.1.
AB036748 mRNA. Translation: BAA89469.1.
AB036749 mRNA. Translation: BAA89470.1.
AK010405 mRNA. Translation: BAB26914.1.
AL663032 Genomic DNA. Translation: CAM19505.1.
AL663032 Genomic DNA. Translation: CAM19507.1.
AL663032 Genomic DNA. Translation: CAM19508.1.
BC032284 mRNA. Translation: AAH32284.1.
CCDSiCCDS29990.1. [Q9JJJ7-4]
CCDS29991.1. [Q9JJJ7-1]
CCDS29992.1. [Q9JJJ7-3]
CCDS29993.1. [Q9JJJ7-2]
RefSeqiNP_001295403.1. NM_001308474.1. [Q9JJJ7-5]
NP_058609.1. NM_016913.4. [Q9JJJ7-4]
NP_076127.1. NM_023638.4. [Q9JJJ7-1]
NP_665914.1. NM_145907.4. [Q9JJJ7-3]
NP_665915.1. NM_145908.4. [Q9JJJ7-2]
UniGeneiMm.443425.

3D structure databases

ProteinModelPortaliQ9JJJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207344. 9 interactors.
STRINGi10090.ENSMUSP00000076790.

Chemistry databases

BindingDBiQ9JJJ7.
ChEMBLiCHEMBL1255164.

PTM databases

PhosphoSitePlusiQ9JJJ7.

Proteomic databases

PaxDbiQ9JJJ7.
PRIDEiQ9JJJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077595; ENSMUSP00000076790; ENSMUSG00000031169. [Q9JJJ7-1]
ENSMUST00000082320; ENSMUSP00000080937; ENSMUSG00000031169. [Q9JJJ7-2]
ENSMUST00000089402; ENSMUSP00000086824; ENSMUSG00000031169. [Q9JJJ7-4]
ENSMUST00000089403; ENSMUSP00000086825; ENSMUSG00000031169. [Q9JJJ7-3]
GeneIDi53627.
KEGGimmu:53627.
UCSCiuc009soj.2. mouse. [Q9JJJ7-1]
uc009sok.2. mouse. [Q9JJJ7-2]
uc009sol.2. mouse. [Q9JJJ7-3]
uc009som.2. mouse. [Q9JJJ7-4]
uc012heo.1. mouse. [Q9JJJ7-5]

Organism-specific databases

CTDi64840.
MGIiMGI:1890212. Porcn.

Phylogenomic databases

eggNOGiKOG4312. Eukaryota.
ENOG410YDZ6. LUCA.
GeneTreeiENSGT00440000033687.
HOVERGENiHBG061243.
InParanoidiQ9JJJ7.
KOiK00181.
OMAiELSWTSH.
OrthoDBiEOG091G0DRB.
PhylomeDBiQ9JJJ7.
TreeFamiTF313724.

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Miscellaneous databases

PROiQ9JJJ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031169.
CleanExiMM_PORCN.
ExpressionAtlasiQ9JJJ7. baseline and differential.
GenevisibleiQ9JJJ7. MM.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPORCN_MOUSE
AccessioniPrimary (citable) accession number: Q9JJJ7
Secondary accession number(s): A2AC30
, A2AC32, A2AC33, Q9CWT0, Q9JJJ8, Q9JJJ9, Q9JJK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was initially thought to mediate palmitoylation of Wnt proteins. It was later shown that it instead acts as a serine O-palmitoleoyltransferase that mediates the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins (PubMed:17141155, PubMed:24798332).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.