Reviewed, UniProtKB/Swiss-Prot Q9JJJ7 (PORCN_MOUSE)

Names and origin

Protein names

Recommended name:
    Probable protein-cysteine N-palmitoyltransferase porcupine
      Short name=mPORC
    EC=2.3.1.-

Gene names

Name:	Porcn
Synonyms:	Porc, Ppn

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Modulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members. Ref.1
Subunit structure	Interacts with WNT1, WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A and WNT7B. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.
Tissue specificity	Expressed in brain, heart, kidney, liver, lung, muscle, spleen and testis. Isoform 4 is strongly expressed in kidney, liver, lung, spleen and testis. Isoform 1 is strongly expressed in brain, heart and muscle and poorly in kidney, liver, lung, spleen and testis. Ref.1
Developmental stage	Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed at different levels in embryo at 9.5, 10.5, 11.5, 12.5, 13.5 and 15.5 dpc. Ref.1
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Porcupine subfamily.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW glycoprotein metabolic process Ref.1 Inferred from direct assay. Source: MGI
Cellular component	integral to endoplasmic reticulum membrane Ref.1 Inferred from direct assay. Source: MGI
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.1 Inferred from physical interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JJJ7-1)

Also known as: D;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JJJ7-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     235-239: Missing.

Isoform 3 (identifier: Q9JJJ7-3)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     229-235: NKKRKAR → K

Isoform 4 (identifier: Q9JJJ7-4)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     229-240: NKKRKARGTMVR → K

Isoform 5 (identifier: Q9JJJ7-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-239: RGTMV → MAQDA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Probable protein-cysteine N-palmitoyltransferase porcupine		PRO_0000213138
Regions
	Topological domain	1 – 17	17	Cytoplasmic Potential
	Transmembrane	18 – 38	21	Potential
	Topological domain	39 – 66	28	Extracellular Potential
	Transmembrane	67 – 87	21	Potential
	Topological domain	88 – 95	8	Cytoplasmic Potential
	Transmembrane	96 – 116	21	Potential
	Topological domain	117 – 152	36	Extracellular Potential
	Transmembrane	153 – 173	21	Potential
	Topological domain	174 – 198	25	Cytoplasmic Potential
	Transmembrane	199 – 219	21	Potential
	Topological domain	220 – 252	33	Extracellular Potential
	Transmembrane	253 – 273	21	Potential
	Topological domain	274 – 337	64	Cytoplasmic Potential
	Transmembrane	338 – 358	21	Potential
	Topological domain	359 – 396	38	Extracellular Potential
	Transmembrane	397 – 417	21	Potential
	Topological domain	418 – 461	44	Cytoplasmic Potential
Sites
	Active site	341	1	Potential
Natural variations
	Alternative sequence	1 – 234	234	Missing in isoform 5.		VSP_015889
	Alternative sequence	229 – 240	12	NKKRK…GTMVR → K in isoform 4.		VSP_015890
	Alternative sequence	229 – 235	7	NKKRKAR → K in isoform 3.		VSP_015891
	Alternative sequence	235 – 239	5	Missing in isoform 2.		VSP_015892
	Alternative sequence	235 – 239	5	RGTMV → MAQDA in isoform 5.		VSP_015893

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (D) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 25A52681C251A0A6	FASTA	461	52,508
	10 20 30 40 50 60 MATFSRQEFF QQLLQGCLLP TVQQGLDQIW LLLTICFACR LLWRLGLPSY LKHASTVAGG 70 80 90 100 110 120 FFSLYHFFQL HMVWVVLLSL LCYLVLFLCR HSSHRGVFLS VTILIYLLMG EMHMVDTVTW 130 140 150 160 170 180 HKMRGAQMIV AMKAVSLGFD LDRGEVGAVP SPVEFMGYLY FVGTIVFGPW ISFHSYLQAV 190 200 210 220 230 240 QGRPLSRRWL KKVARSLALA LLCLVLSTCV GPYLFPYFIP LDGDRLLRNK KRKARGTMVR 250 260 270 280 290 300 WLRAYESAVS FHFSNYFVGF LSEATATLAG AGFTEEKDHL EWDLTVSRPL NVELPRSMVE 310 320 330 340 350 360 VVTSWNLPMS YWLNNYVFKN ALRLGTFSAV LVTYAASALL HGFSFHLAAV LLSLAFITYV 370 380 390 400 410 420 EHVLRKRLAQ ILSACILSKR CLPDCSHRHR LGLGVRALNL LFGALAIFHL SYLGSLFDVD 430 440 450 460 VDDTTEEQGY GMAYTVHKWS ELSWASHWVT FGCWIFYRLI G
	Isoform 2 (B). Checksum: 2903AD4BD0CA2184	FASTA	456	51,963
	10 20 30 40 50 60 MATFSRQEFF QQLLQGCLLP TVQQGLDQIW LLLTICFACR LLWRLGLPSY LKHASTVAGG 70 80 90 100 110 120 FFSLYHFFQL HMVWVVLLSL LCYLVLFLCR HSSHRGVFLS VTILIYLLMG EMHMVDTVTW 130 140 150 160 170 180 HKMRGAQMIV AMKAVSLGFD LDRGEVGAVP SPVEFMGYLY FVGTIVFGPW ISFHSYLQAV 190 200 210 220 230 240 QGRPLSRRWL KKVARSLALA LLCLVLSTCV GPYLFPYFIP LDGDRLLRNK KRKARWLRAY 250 260 270 280 290 300 ESAVSFHFSN YFVGFLSEAT ATLAGAGFTE EKDHLEWDLT VSRPLNVELP RSMVEVVTSW 310 320 330 340 350 360 NLPMSYWLNN YVFKNALRLG TFSAVLVTYA ASALLHGFSF HLAAVLLSLA FITYVEHVLR 370 380 390 400 410 420 KRLAQILSAC ILSKRCLPDC SHRHRLGLGV RALNLLFGAL AIFHLSYLGS LFDVDVDDTT 430 440 450 EEQGYGMAYT VHKWSELSWA SHWVTFGCWI FYRLIG
	Isoform 3 (C). Checksum: A78DCC9613A18319	FASTA	455	51,754
	10 20 30 40 50 60 MATFSRQEFF QQLLQGCLLP TVQQGLDQIW LLLTICFACR LLWRLGLPSY LKHASTVAGG 70 80 90 100 110 120 FFSLYHFFQL HMVWVVLLSL LCYLVLFLCR HSSHRGVFLS VTILIYLLMG EMHMVDTVTW 130 140 150 160 170 180 HKMRGAQMIV AMKAVSLGFD LDRGEVGAVP SPVEFMGYLY FVGTIVFGPW ISFHSYLQAV 190 200 210 220 230 240 QGRPLSRRWL KKVARSLALA LLCLVLSTCV GPYLFPYFIP LDGDRLLRKG TMVRWLRAYE 250 260 270 280 290 300 SAVSFHFSNY FVGFLSEATA TLAGAGFTEE KDHLEWDLTV SRPLNVELPR SMVEVVTSWN 310 320 330 340 350 360 LPMSYWLNNY VFKNALRLGT FSAVLVTYAA SALLHGFSFH LAAVLLSLAF ITYVEHVLRK 370 380 390 400 410 420 RLAQILSACI LSKRCLPDCS HRHRLGLGVR ALNLLFGALA IFHLSYLGSL FDVDVDDTTE 430 440 450 EQGYGMAYTV HKWSELSWAS HWVTFGCWIF YRLIG
	Isoform 4 (A). Checksum: 992012ABE6B649E8	FASTA	450	51,209
	10 20 30 40 50 60 MATFSRQEFF QQLLQGCLLP TVQQGLDQIW LLLTICFACR LLWRLGLPSY LKHASTVAGG 70 80 90 100 110 120 FFSLYHFFQL HMVWVVLLSL LCYLVLFLCR HSSHRGVFLS VTILIYLLMG EMHMVDTVTW 130 140 150 160 170 180 HKMRGAQMIV AMKAVSLGFD LDRGEVGAVP SPVEFMGYLY FVGTIVFGPW ISFHSYLQAV 190 200 210 220 230 240 QGRPLSRRWL KKVARSLALA LLCLVLSTCV GPYLFPYFIP LDGDRLLRKW LRAYESAVSF 250 260 270 280 290 300 HFSNYFVGFL SEATATLAGA GFTEEKDHLE WDLTVSRPLN VELPRSMVEV VTSWNLPMSY 310 320 330 340 350 360 WLNNYVFKNA LRLGTFSAVL VTYAASALLH GFSFHLAAVL LSLAFITYVE HVLRKRLAQI 370 380 390 400 410 420 LSACILSKRC LPDCSHRHRL GLGVRALNLL FGALAIFHLS YLGSLFDVDV DDTTEEQGYG 430 440 450 MAYTVHKWSE LSWASHWVTF GCWIFYRLIG
	Isoform 5. Checksum: 4B78150917A1685B	FASTA	227	25,726
	10 20 30 40 50 60 MAQDARWLRA YESAVSFHFS NYFVGFLSEA TATLAGAGFT EEKDHLEWDL TVSRPLNVEL 70 80 90 100 110 120 PRSMVEVVTS WNLPMSYWLN NYVFKNALRL GTFSAVLVTY AASALLHGFS FHLAAVLLSL 130 140 150 160 170 180 AFITYVEHVL RKRLAQILSA CILSKRCLPD CSHRHRLGLG VRALNLLFGA LAIFHLSYLG 190 200 210 220 SLFDVDVDDT TEEQGYGMAY TVHKWSELSW ASHWVTFGCW IFYRLIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed: 10866835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION IN WNT PROTEINS PROCESSING, INTERACTION WITH WNT1; WNT3; WNT3A; WNT4; WNT5A; WNT5B; WNT6; WNT7A AND WNT7B, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Strain: C57BL/6J. Tissue: Embryonic stem cell.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6. Tissue: Eye.
[5]	"Misexpression of mouse porcupine isoforms modulates the differentiation of P19 embryonic carcinoma cells." Tanaka K., Kitagawa Y., Kadowaki T. Cell Biol. Int. 27:549-557(2003) [PubMed: 12842094] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3 AND 4).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB036746 mRNA. Translation: BAA89467.1. AB036747 mRNA. Translation: BAA89468.1. AB036748 mRNA. Translation: BAA89469.1. AB036749 mRNA. Translation: BAA89470.1. AK010405 mRNA. Translation: BAB26914.1. AL663032 Genomic DNA. Translation: CAM19505.1. AL663032 Genomic DNA. Translation: CAM19507.1. AL663032 Genomic DNA. Translation: CAM19508.1. BC032284 mRNA. Translation: AAH32284.1.
IPI	IPI00121914. IPI00162937. IPI00162939. IPI00162988. IPI00655226.
RefSeq	NP_058609.1. NP_076127.1. NP_665914.1. NP_665915.1.
UniGene	Mm.443425
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JJJ7.
Proteomic databases
PRIDE	Q9JJJ7.
Genome annotation databases
Ensembl	ENSMUST00000077595; ENSMUSP00000076790; ENSMUSG00000031169; Mus musculus. [Genome view] ENSMUST00000082320; ENSMUSP00000080937; ENSMUSG00000031169; Mus musculus. [Genome view] ENSMUST00000089402; ENSMUSP00000086824; ENSMUSG00000031169; Mus musculus. [Genome view] ENSMUST00000089403; ENSMUSP00000086825; ENSMUSG00000031169; Mus musculus. [Genome view] ENSMUST00000115602; ENSMUSP00000111265; ENSMUSG00000031169; Mus musculus. [Genome view]
GeneID	53627.
KEGG	mmu:53627.
UCSC	uc009soj.1. mouse. uc009sok.1. mouse. uc009sol.1. mouse. uc009som.1. mouse.
Organism-specific databases
CTD	53627.
MGI	MGI:1890212. Porcn.
Phylogenomic databases
HOGENOM	HBG446923.
HOVERGEN	Q9JJJ7.
InParanoid	Q9JJJ7.
OMA	CVAPYLF.
PhylomeDB	Q9JJJ7.
Gene expression databases
ArrayExpress	Q9JJJ7.
Bgee	Q9JJJ7.
CleanEx	MM_PORCN.
Genevestigator	Q9JJJ7.
GermOnline	ENSMUSG00000031169. Mus musculus.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	310377.
SOURCE	Search...

Entry information

Entry name

PORCN_MOUSE

Accession

Primary (citable) accession number: Q9JJJ7
Secondary accession number(s): A2AC30 , A2AC32, A2AC33, Q9CWT0, Q9JJJ8, Q9JJJ9, Q9JJK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)