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Q9JJH7 (TRPM5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel subfamily M member 5
Alternative name(s):
Long transient receptor potential channel 5
Short name=LTrpC-5
Short name=LTrpC5
MLSN1- and TRP-related gene 1 protein
Gene names
Name:Trpm5
Synonyms:Ltrpc5, Mtr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage-modulated Ca2+-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na+, K+ and Cs+ ions equally well. Activated directly by increases in intracellular Ca2+, but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca2+. Also activated by a fast intracellular Ca2+ increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Ref.4 Ref.8 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15 Ref.16

Enzyme regulation

Phosphatidylinositol 4,5-bisphosphate (PIP2) is able to partially restore sensitivity to Ca2+ after desensitization. Inhibited by flufenamic acid with an IC50 of 24.5 µM and spermine with an IC50 of 37 µM. Ref.8 Ref.9

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Tissue specificity

Strongly expressed in liver, heart, testis, brain and kidney. Detected in fetal liver, kidney, spleen, brain, heart and lung, and in adult skin, eyes, spleen, stomach, small intestine, colon, lung, bladder, pancreas and thymus. Biallelically expressed at all stages and tissues examined. Also expressed in subsets of taste receptor cells of the tongue, in olfactory sensory neurons of the main olfactory epithelium and in the vomeronasal organ. Ref.2 Ref.7 Ref.14 Ref.16

Disruption phenotype

Mice show diminished behavioral and nerve responses to bitter, sweet and umami tastes. Ref.13

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily. [View classification]

Sequence caution

The sequence CAB94717.2 differs from that shown. Reason: Frameshift at position 1130.

The sequence CAC19456.1 differs from that shown. Reason: Frameshift at position 1130.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 (identifier: Q9JJH7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.3 (identifier: Q9JJH7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1108-1116: ANYCMLLLS → SKYGFRPWE
     1117-1158: Missing.
Isoform 3 Ref.4 (identifier: Q9JJH7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     978-1000: SYTFQVVQGNADMFWKFQRYHLI → RVLTETGPMSWYFAAVSSGLDLQ
     1001-1158: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11581158Transient receptor potential cation channel subfamily M member 5
PRO_0000328934

Regions

Topological domain1 – 645645Cytoplasmic Potential
Transmembrane646 – 66621Helical; Potential
Topological domain667 – 73266Extracellular Potential
Transmembrane733 – 75321Helical; Potential
Topological domain754 – 81057Cytoplasmic Potential
Transmembrane811 – 83121Helical; Potential
Topological domain832 – 8343Extracellular Potential
Transmembrane835 – 85521Helical; Potential
Topological domain856 – 87116Cytoplasmic Potential
Transmembrane872 – 89221Helical; Potential
Topological domain893 – 95361Extracellular Potential
Transmembrane954 – 97421Helical; Potential
Topological domain975 – 1158184Cytoplasmic Potential
Coiled coil552 – 57221 Potential

Natural variations

Alternative sequence978 – 100023SYTFQ…RYHLI → RVLTETGPMSWYFAAVSSGL DLQ in isoform 3. Ref.4
VSP_052744
Alternative sequence1001 – 1158158Missing in isoform 3. Ref.4
VSP_052745
Alternative sequence1108 – 11169ANYCMLLLS → SKYGFRPWE in isoform 2. Ref.3
VSP_052746
Alternative sequence1117 – 115842Missing in isoform 2. Ref.3
VSP_052747

Experimental info

Mutagenesis8311E → Q: Reduces sensitivity to block by extracellular acidification. Sensitivity to block by low extracellular pH is nearly abolished; when associated with N-935. Ref.10
Mutagenesis8971H → N: Shows normal sensitivity to acid block and significant recovery from acid-enhanced inactivation. Ref.10
Mutagenesis9351H → N: Reduces sensitivity to block by extracellular acidification. Sensitivity to block by low extracellular pH is nearly abolished; when associated with Q-831. Ref.10
Sequence conflict241C → S in AAI33713. Ref.5
Sequence conflict6121H → P in CAB94717. Ref.3
Sequence conflict8711D → E in BAE36157. Ref.6
Sequence conflict8971H → N in AAI33713. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F0A5237EC67867CE

FASTA1,158130,844
        10         20         30         40         50         60 
MQTTQSSCPG SPPDTEDGWE PILCRGEINF GGSGKKRGKF VKVPSSVAPS VLFELLLTEW 

        70         80         90        100        110        120 
HLPAPNLVVS LVGEERPLAM KSWLRDVLRK GLVKAAQSTG AWILTSALHV GLARHVGQAV 

       130        140        150        160        170        180 
RDHSLASTST KIRVVAIGMA SLDRILHRQL LDGVHQKEDT PIHYPADEGN IQGPLCPLDS 

       190        200        210        220        230        240 
NLSHFILVES GALGSGNDGL TELQLSLEKH ISQQRTGYGG TSCIQIPVLC LLVNGDPNTL 

       250        260        270        280        290        300 
ERISRAVEQA APWLILAGSG GIADVLAALV SQPHLLVPQV AEKQFREKFP SECFSWEAIV 

       310        320        330        340        350        360 
HWTELLQNIA AHPHLLTVYD FEQEGSEDLD TVILKALVKA CKSHSQEAQD YLDELKLAVA 

       370        380        390        400        410        420 
WDRVDIAKSE IFNGDVEWKS CDLEEVMTDA LVSNKPDFVR LFVDSGADMA EFLTYGRLQQ 

       430        440        450        460        470        480 
LYHSVSPKSL LFELLQRKHE EGRLTLAGLG AQQARELPIG LPAFSLHEVS RVLKDFLHDA 

       490        500        510        520        530        540 
CRGFYQDGRR MEERGPPKRP AGQKWLPDLS RKSEDPWRDL FLWAVLQNRY EMATYFWAMG 

       550        560        570        580        590        600 
REGVAAALAA CKIIKEMSHL EKEAEVARTM REAKYEQLAL DLFSECYGNS EDRAFALLVR 

       610        620        630        640        650        660 
RNHSWSRTTC LHLATEADAK AFFAHDGVQA FLTKIWWGDM ATGTPILRLL GAFTCPALIY 

       670        680        690        700        710        720 
TNLISFSEDA PQRMDLEDLQ EPDSLDMEKS FLCSRGGQLE KLTEAPRAPG DLGPQAAFLL 

       730        740        750        760        770        780 
TRWRKFWGAP VTVFLGNVVM YFAFLFLFTY VLLVDFRPPP QGPSGSEVTL YFWVFTLVLE 

       790        800        810        820        830        840 
EIRQGFFTDE DTHLVKKFTL YVEDNWNKCD MVAIFLFIVG VTCRMVPSVF EAGRTVLAID 

       850        860        870        880        890        900 
FMVFTLRLIH IFAIHKQLGP KIIIVERMMK DVFFFLFFLS VWLVAYGVTT QALLHPHDGR 

       910        920        930        940        950        960 
LEWIFRRVLY RPYLQIFGQI PLDEIDEARV NCSLHPLLLE SSASCPNLYA NWLVILLLVT 

       970        980        990       1000       1010       1020 
FLLVTNVLLM NLLIAMFSYT FQVVQGNADM FWKFQRYHLI VEYHGRPALA PPFILLSHLS 

      1030       1040       1050       1060       1070       1080 
LVLKQVFRKE AQHKRQHLER DLPDPLDQKI ITWETVQKEN FLSTMEKRRR DSEGEVLRKT 

      1090       1100       1110       1120       1130       1140 
AHRVDLIAKY IGGLREQEKR IKCLESQANY CMLLLSSMTD TLAPGGTYSS SQNCGCRSQP 

      1150 
ASARDREYLE SGLPPSDT 

« Hide

Isoform 2 [UniParc].

Checksum: 32DFFABCF2835E48
Show »

FASTA1,116126,609
Isoform 3 [UniParc].

Checksum: BB107610176E6660
Show »

FASTA1,000112,460

References

« Hide 'large scale' references
[1]"Sequence-based structural features between Kvlqt1 and Tapa1 on mouse chromosome 7F4/F5 corresponding to the Beckwith-Wiedemann syndrome region on human 11p15.5: long-stretches of unusually well conserved intronic sequences of kvlqt1 between mouse and human."
Yatsuki H., Watanabe H., Hattori M., Joh K., Soejima H., Komoda H., Xin Z., Zhu X., Higashimoto K., Nishimura M., Kuratomi S., Sasaki H., Sakaki Y., Mukai T.
DNA Res. 7:195-206(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Mtr1, a novel biallelically expressed gene in the center of the mouse distal chromosome 7 imprinting cluster, is a member of the Trp gene family."
Enklaar T., Esswein M., Oswald M., Hilbert K., Winterpacht A., Higgins M., Zabel B., Prawitt D.
Genomics 67:179-187(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]"Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse."
Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K., Reinhardt R., Reik W., Walter J.
Hum. Mol. Genet. 9:1829-1841(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
Strain: 129/Sv.
[4]"TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel."
Hofmann T., Chubanov V., Gudermann T., Montell C.
Curr. Biol. 13:1153-1158(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), FUNCTION.
Tissue: Testis.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 441-1158 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryonic liver.
[7]"A transient receptor potential channel expressed in taste receptor cells."
Perez C.A., Huang L., Rong M., Kozak J.A., Preuss A.K., Zhang H., Max M., Margolskee R.F.
Nat. Neurosci. 5:1169-1176(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5."
Liu D., Liman E.R.
Proc. Natl. Acad. Sci. U.S.A. 100:15160-15165(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[9]"Comparison of functional properties of the Ca2+-activated cation channels TRPM4 and TRPM5 from mice."
Ullrich N.D., Voets T., Prenen J., Vennekens R., Talavera K., Droogmans G., Nilius B.
Cell Calcium 37:267-278(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[10]"Extracellular acid block and acid-enhanced inactivation of the Ca2+-activated cation channel TRPM5 involve residues in the S3-S4 and S5-S6 extracellular domains."
Liu D., Zhang Z., Liman E.R.
J. Biol. Chem. 280:20691-20699(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-831; HIS-897 AND HIS-935.
[11]"Heat activation of TRPM5 underlies thermal sensitivity of sweet taste."
Talavera K., Yasumatsu K., Voets T., Droogmans G., Shigemura N., Ninomiya Y., Margolskee R.F., Nilius B.
Nature 438:1022-1025(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Arachidonic acid can function as a signaling modulator by activating the TRPM5 cation channel in taste receptor cells."
Oike H., Wakamori M., Mori Y., Nakanishi H., Taguchi R., Misaka T., Matsumoto I., Abe K.
Biochim. Biophys. Acta 1761:1078-1084(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Trpm5 null mice respond to bitter, sweet, and umami compounds."
Damak S., Rong M., Yasumatsu K., Kokrashvili Z., Perez C.A., Shigemura N., Yoshida R., Mosinger B. Jr., Glendinning J.I., Ninomiya Y., Margolskee R.F.
Chem. Senses 31:253-264(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[14]"TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells."
Kaske S., Krasteva G., Koenig P., Kummer W., Hofmann T., Gudermann T., Chubanov V.
BMC Neurosci. 8:49-49(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[15]"The transduction channel TRPM5 is gated by intracellular calcium in taste cells."
Zhang Z., Zhao Z., Margolskee R., Liman E.
J. Neurosci. 27:5777-5786(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"Olfactory neurons expressing transient receptor potential channel M5 (TRPM5) are involved in sensing semiochemicals."
Lin W., Margolskee R., Donnert G., Hell S.W., Restrepo D.
Proc. Natl. Acad. Sci. U.S.A. 104:2471-2476(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB039952 mRNA. Translation: BAA96877.1.
AF228681 mRNA. Translation: AAF98120.1.
AJ251835 Genomic DNA. Translation: CAC19456.1. Frameshift.
AJ251835 Genomic DNA. Translation: CAC19457.1.
AJ271092 mRNA. Translation: CAB94717.2. Frameshift.
AY280364 mRNA. Translation: AAP44476.1.
AY280365 mRNA. Translation: AAP44477.1.
BC133712 mRNA. Translation: AAI33713.1.
AK161030 mRNA. Translation: BAE36157.1.
CCDSCCDS52462.1. [Q9JJH7-1]
RefSeqNP_064673.2. NM_020277.2. [Q9JJH7-1]
XP_006508708.1. XM_006508645.1. [Q9JJH7-1]
XP_006508709.1. XM_006508646.1. [Q9JJH7-3]
UniGeneMm.286668.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY497.

PTM databases

PhosphoSiteQ9JJH7.

Proteomic databases

PRIDEQ9JJH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009390; ENSMUSP00000009390; ENSMUSG00000009246. [Q9JJH7-1]
ENSMUST00000150867; ENSMUSP00000114302; ENSMUSG00000009246. [Q9JJH7-3]
GeneID56843.
KEGGmmu:56843.
UCSCuc009koz.1. mouse. [Q9JJH7-1]

Organism-specific databases

CTD29850.
MGIMGI:1861718. Trpm5.

Phylogenomic databases

eggNOGNOG253824.
GeneTreeENSGT00650000093200.
HOVERGENHBG108337.
InParanoidQ9JJH7.
KOK04980.
OMAECYSNSE.
OrthoDBEOG725DH1.
PhylomeDBQ9JJH7.
TreeFamTF314204.

Gene expression databases

BgeeQ9JJH7.
GenevestigatorQ9JJH7.

Family and domain databases

ProtoNetSearch...

Other

NextBio313391.
PROQ9JJH7.
SOURCESearch...

Entry information

Entry nameTRPM5_MOUSE
AccessionPrimary (citable) accession number: Q9JJH7
Secondary accession number(s): A3KN89 expand/collapse secondary AC list , Q3TU14, Q7TPL4, Q99NF9, Q9EPM3, Q9EPM4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot