6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Alternative name(s):
    PFK-2/FBPase-2
    6PF-2-K/Fru-2,6-P2ASE heart-type isozyme
    RH2K
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:

Pfkfb2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H₂O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

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Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Traceable author statement. Source: RGD glucose catabolic process Inferred from direct assay. Source: RGD lactate metabolic process Inferred from direct assay. Source: RGD positive regulation of glucokinase activity Inferred from direct assay. Source: RGD positive regulation of insulin secretion Inferred from direct assay. Source: RGD pyruvate metabolic process Inferred from direct assay. Source: RGD response to glucose stimulus Inferred from direct assay. Source: RGD
Cellular component	cytoplasm Inferred from direct assay. Source: RGD
Molecular function	6-phosphofructo-2-kinase activity Traceable author statement. Source: RGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Traceable author statement. Source: RGD kinase binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: Q9JJH5-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9JJH5-2) The sequence of this isoform differs from the canonical sequence as follows: 515-519: QQGSA → SIPVV 520-547: Missing.

Isoform 2 (identifier: Q9JJH5-3) The sequence of this isoform differs from the canonical sequence as follows: 514-536: Missing.

Isoform 4 (identifier: Q9JJH5-4) The sequence of this isoform differs from the canonical sequence as follows: 454-536: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 557

557

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2

PRO_0000179966

Regions

Nucleotide binding

48 – 55

8

ATP Potential

Region

1 – 251

251

6-phosphofructo-2-kinase

Region

252 – 557

306

Fructose-2,6-bisphosphatase

Sites

Active site

131

1

Potential

Active site

161

1

Potential

Active site

260

1

Tele-phosphohistidine intermediate By similarity

Active site

329

1

Potential

Active site

394

1

Proton donor By similarity

Binding site

105

1

Fructose-6-phosphate By similarity

Binding site

196

1

Fructose-6-phosphate By similarity

Amino acid modifications

Modified residue

32

1

Phosphoserine; by PKA By similarity

Modified residue

469

1

Phosphoserine; by PKA By similarity

Modified residue

471

1

Phosphothreonine By similarity

Modified residue

478

1

Phosphothreonine; by PKC By similarity

Modified residue

486

1

Phosphoserine By similarity

Modified residue

496

1

Phosphoserine By similarity

Natural variations

Alternative sequence

454 – 536

83

Missing in isoform 4.

VSP_004678

Alternative sequence

514 – 536

23

Missing in isoform 2.

VSP_004676

Alternative sequence

515 – 519

5

QQGSA → SIPVV in isoform 1.

VSP_004677

Alternative sequence

520 – 547

28

Missing in isoform 1.

VSP_004679

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Sequences

Sequence		Length	Mass (Da)
Isoform 3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D5707054E416629F Show »	FASTA	557	64,156
10 20 30 40 50 60 MSENSTFSTE DSSSSSYKPH ASNLRRAGKK CSWASYMTNS PTLIVMIGLP ARGKTYVSKK 70 80 90 100 110 120 LTRYLNWIGV PTKVFNLGVY RREAVKSYKS YDFFRHDNEE AMKIRKQCAL VALEDVKAYF 130 140 150 160 170 180 TEESGQIAVF DATNTTRERR DMILNFAKQN AFKVFFVESV CDDPDVIAAN ILEVKVSSPD 190 200 210 220 230 240 YPERNRENVM EDFLKRIECY KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK 250 260 270 280 290 300 IVYYLMNIHV HPRTIYLCRH GESEFNLLGK IGGDSGLSLR GKQFAQALKK FLEEQEIQDL 310 320 330 340 350 360 KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE FALRDQEKYL 370 380 390 400 410 420 YRYPGGESYQ DLVQRLEPVI MELERQGNVL VISHQAVMRC LLAYFLDKGA DELPYLRCPL 430 440 450 460 470 480 HIIFKLTPVA YGCKVETITL NVEAVDTHRD KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR 490 500 510 520 530 540 RPRNYSVGSR PLKPLSPLRA LDMQEGADQP KTQVQQGSAQ ATEHLQKALE FANGHREVEN 550 VLAKHRRPSM ASLTLLS « Hide
Isoform 1. Checksum: 013AABF875C60840 Show »	FASTA	529	60,916
10 20 30 40 50 60 MSENSTFSTE DSSSSSYKPH ASNLRRAGKK CSWASYMTNS PTLIVMIGLP ARGKTYVSKK 70 80 90 100 110 120 LTRYLNWIGV PTKVFNLGVY RREAVKSYKS YDFFRHDNEE AMKIRKQCAL VALEDVKAYF 130 140 150 160 170 180 TEESGQIAVF DATNTTRERR DMILNFAKQN AFKVFFVESV CDDPDVIAAN ILEVKVSSPD 190 200 210 220 230 240 YPERNRENVM EDFLKRIECY KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK 250 260 270 280 290 300 IVYYLMNIHV HPRTIYLCRH GESEFNLLGK IGGDSGLSLR GKQFAQALKK FLEEQEIQDL 310 320 330 340 350 360 KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE FALRDQEKYL 370 380 390 400 410 420 YRYPGGESYQ DLVQRLEPVI MELERQGNVL VISHQAVMRC LLAYFLDKGA DELPYLRCPL 430 440 450 460 470 480 HIIFKLTPVA YGCKVETITL NVEAVDTHRD KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR 490 500 510 520 RPRNYSVGSR PLKPLSPLRA LDMQEGADQP KTQVSIPVVP SMASLTLLS « Hide
Isoform 2. Checksum: 88C635DE3964CD53 Show »	FASTA	534	61,653
10 20 30 40 50 60 MSENSTFSTE DSSSSSYKPH ASNLRRAGKK CSWASYMTNS PTLIVMIGLP ARGKTYVSKK 70 80 90 100 110 120 LTRYLNWIGV PTKVFNLGVY RREAVKSYKS YDFFRHDNEE AMKIRKQCAL VALEDVKAYF 130 140 150 160 170 180 TEESGQIAVF DATNTTRERR DMILNFAKQN AFKVFFVESV CDDPDVIAAN ILEVKVSSPD 190 200 210 220 230 240 YPERNRENVM EDFLKRIECY KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK 250 260 270 280 290 300 IVYYLMNIHV HPRTIYLCRH GESEFNLLGK IGGDSGLSLR GKQFAQALKK FLEEQEIQDL 310 320 330 340 350 360 KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE FALRDQEKYL 370 380 390 400 410 420 YRYPGGESYQ DLVQRLEPVI MELERQGNVL VISHQAVMRC LLAYFLDKGA DELPYLRCPL 430 440 450 460 470 480 HIIFKLTPVA YGCKVETITL NVEAVDTHRD KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR 490 500 510 520 530 RPRNYSVGSR PLKPLSPLRA LDMQEGADQP KTQEVENVLA KHRRPSMASL TLLS « Hide
Isoform 4. Checksum: 1EDFF196B219764D Show »	FASTA	474	54,823
10 20 30 40 50 60 MSENSTFSTE DSSSSSYKPH ASNLRRAGKK CSWASYMTNS PTLIVMIGLP ARGKTYVSKK 70 80 90 100 110 120 LTRYLNWIGV PTKVFNLGVY RREAVKSYKS YDFFRHDNEE AMKIRKQCAL VALEDVKAYF 130 140 150 160 170 180 TEESGQIAVF DATNTTRERR DMILNFAKQN AFKVFFVESV CDDPDVIAAN ILEVKVSSPD 190 200 210 220 230 240 YPERNRENVM EDFLKRIECY KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK 250 260 270 280 290 300 IVYYLMNIHV HPRTIYLCRH GESEFNLLGK IGGDSGLSLR GKQFAQALKK FLEEQEIQDL 310 320 330 340 350 360 KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE FALRDQEKYL 370 380 390 400 410 420 YRYPGGESYQ DLVQRLEPVI MELERQGNVL VISHQAVMRC LLAYFLDKGA DELPYLRCPL 430 440 450 460 470 HIIFKLTPVA YGCKVETITL NVEAVDTHRD KPTEVENVLA KHRRPSMASL TLLS « Hide

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References

[1]	"Molecular cloning and tissue specific expression of fructose 6-phosphate,2-kinase:fructose 2,6-bisphosphatase of rat brain." Watanabe F., Sakai A., Furuya E., Uyeda K. Biochem. Biophys. Res. Commun. 198:335-340(1994) [PubMed: 8292038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"New isoforms of rat heart-type fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase are generated by alternative splicing of novel exons." Watanabe F., Furuya E. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	S67900 mRNA. Translation: AAB29678.1. L27084 mRNA. Translation: AAA41132.1. AB040530 mRNA. Translation: BAA96495.1. AB040531 mRNA. Translation: BAA96496.1. AB040532 mRNA. Translation: BAA96497.1. AB040533 mRNA. Translation: BAA96498.1.
IPI	IPI00201591. IPI00230808. IPI00230809. IPI00230810.
PIR	JC2037.
RefSeq	NP_001029136.1. NP_001029137.1. NP_536725.2.
UniGene	Rn.44844
3D structure databases
HSSP	HSSP built from PDB template 1C80 based on UniProtKB P07953.
ModBase	Search...
PTM databases
PhosphoSite	Q9JJH5.
Genome annotation databases
Ensembl	ENSRNOG00000004162. Rattus norvegicus. [Contig view]
GeneID	24640.
KEGG	rno:24640.
NMPDR	fig\|10116.3.peg.9026.
Organism-specific databases
RGD	3309. Pfkfb2.
Phylogenomic databases
HOVERGEN	Q9JJH5.
OMA	Q9JJH5. HPRTIYL.
Enzyme and pathway databases
BRENDA	2.7.1.105. 248. 3.1.3.46. 248.
Gene expression databases
ArrayExpress	Q9JJH5.
GermOnline	ENSRNOG00000004162. Rattus norvegicus.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603930.

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Entry information

Entry name

F262_RAT

Accession

Primary (citable) accession number: Q9JJH5
Secondary accession number(s): Q64297, Q9JHL5, Q9JJH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents