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Protein

Sperm-associated antigen 4 protein

Gene

Spag4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 4 protein
Alternative name(s):
Outer dense fiber-associated protein SPAG4
SUN domain-containing protein 4
Gene namesi
Name:Spag4
ORF Names:MNCb-0953, Sun4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444120. Spag4.

Subcellular locationi

Isoform 1 :
  • Membrane Curated; Multi-pass membrane protein Curated
  • Cytoplasmcytoskeleton By similarity
  • Nucleus envelope 1 Publication
  • Nucleus inner membrane Curated
  • Cytoplasmcytoskeletonflagellum axoneme By similarity

  • Note: In spermatids, isoform 1 is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm (By similarity). Conflictingly is not found in axoneme but only associated with the manchette where cytoplasmic microtubules contact the nuclear envelope. Localizes at the posterior lateral side of round and elongating spermatids (PubMed:26621829).By similarity1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Male infertility with severe defects in sperm head formation and globozoospermia-like phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189171 – 443Sperm-associated antigen 4 proteinAdd BLAST443

Proteomic databases

PaxDbiQ9JJF2.
PRIDEiQ9JJF2.

PTM databases

PhosphoSitePlusiQ9JJF2.

Expressioni

Tissue specificityi

Isoform 1 is testis specific and is exclusively expressed in spermatids.2 Publications

Developmental stagei

Weakly expressed at day 18 p.p. and increased expression at day 21-25 p.p. with increasing spermatid numbers.1 Publication

Gene expression databases

BgeeiENSMUSG00000038180.
CleanExiMM_SPAG4.
ExpressionAtlasiQ9JJF2. baseline and differential.
GenevisibleiQ9JJF2. MM.

Interactioni

Subunit structurei

Self-associates. Interacts with ODF1. May associate with microtubules (By similarity). Interacts with SUN3 and SYNE1; suggesting the formation of a spermatogenesis-specific LINC complex; a SUN domain-based heterotrimer of SPAG4 and SUN3 may associate with SYNE1 (PubMed:26621829). Interacts with SEPT12 and LMNB1; during spermatogenesis (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9JJF2. 2 interactors.
STRINGi10090.ENSMUSP00000036484.

Structurei

3D structure databases

ProteinModelPortaliQ9JJF2.
SMRiQ9JJF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini267 – 427SUNPROSITE-ProRule annotationAdd BLAST161

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili203 – 244Sequence analysisAdd BLAST42

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
GeneTreeiENSGT00390000011587.
HOGENOMiHOG000246956.
HOVERGENiHBG079205.
InParanoidiQ9JJF2.
OMAiSVYREVC.
OrthoDBiEOG091G0AZ8.
TreeFamiTF323915.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiView protein in InterPro
IPR008979. Galactose-bd-like.
IPR027776. SPAG4/SUN4.
IPR012919. SUN_dom.
PANTHERiPTHR12911:SF28. PTHR12911:SF28. 1 hit.
PfamiView protein in Pfam
PF07738. Sad1_UNC. 1 hit.
PROSITEiView protein in PROSITE
PS51469. SUN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJF2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRSPRSGSA ASSHNHTPNF YSENSNSSHS ATSGDSNGRR SAGPELGEPE
60 70 80 90 100
GRRARGSSCG EPALSPGMPG GDTWAGSSRP KLAPRSHNGQ TACGAATVRG
110 120 130 140 150
GASEPSGSSV VLEEQLNLLP ILDLRQEMPT PRVSKSFLSL LFQVLSMVLS
160 170 180 190 200
LAVDGLVCVC REICSIRFLF TAVSLLSIFL AALWWGLLYL IPPLENEPTE
210 220 230 240 250
MLTLSQYHHR VHSQGQQLQQ LQAELNKLHK EVSSVRAAHS ERVAKLVFQR
260 270 280 290 300
LNEDFVRKPD YALSSVGASI DLEKTSSDYE DQNTAYFWNR LSFWNYARPP
310 320 330 340 350
SVILEPDVFP GNCWAFEGDK GQVVIRLPGH VQLSDITLQH PPPTVAHTGG
360 370 380 390 400
ASSAPRDFAV YGLQADDETE VFLGKFIFDV QKSEIQTFHL QNDPPSAFPK
410 420 430 440
VKIQILSNWG HPRFTCLYRV RAHGVRTSEW ADDNATGVTG GPH
Note: Derived from EST data.
Length:443
Mass (Da):48,582
Last modified:July 27, 2011 - v3
Checksum:iF5B8BD2F57E5F200
GO
Isoform 2 (identifier: Q9JJF2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-103: VRGGAS → NRLDLL
     104-443: Missing.

Show »
Length:103
Mass (Da):10,589
Checksum:i06707EEA59ED8972
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81K → E in BAA95039 (Ref. 1) Curated1
Sequence conflicti259P → T in BAA95039 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00595898 – 103VRGGAS → NRLDLL in isoform 2. 1 Publication6
Alternative sequenceiVSP_005959104 – 443Missing in isoform 2. 1 PublicationAdd BLAST340

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041554 mRNA. Translation: BAA95039.1.
AL833786 Genomic DNA. Translation: CAM46026.1.
CH466551 Genomic DNA. Translation: EDL06167.1.
BB610287 mRNA. No translation available.
BU937622 mRNA. No translation available.
CA466584 mRNA. No translation available.
CCDSiCCDS50777.1. [Q9JJF2-1]
RefSeqiNP_631890.3. NM_139151.4. [Q9JJF2-1]
UniGeneiMm.330713.

Genome annotation databases

EnsembliENSMUST00000038860; ENSMUSP00000036484; ENSMUSG00000038180. [Q9JJF2-1]
GeneIDi245865.
KEGGimmu:245865.
UCSCiuc008nmc.1. mouse. [Q9JJF2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSPAG4_MOUSE
AccessioniPrimary (citable) accession number: Q9JJF2
Secondary accession number(s): A3KGK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: July 27, 2011
Last modified: July 5, 2017
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although transmembrane domains are strongly predicted, they may rather represent hydrophobic globular domains associated with microtubules.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot