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Protein

Sperm-associated antigen 4 protein

Gene

Spag4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 4 protein
Alternative name(s):
Outer dense fiber-associated protein SPAG4
SUN domain-containing protein 4
Gene namesi
Name:Spag4
ORF Names:MNCb-0953, Sun4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444120. Spag4.

Subcellular locationi

Isoform 1 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei137 – 15721HelicalSequence analysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Sperm-associated antigen 4 proteinPRO_0000218917Add
BLAST

Proteomic databases

PaxDbiQ9JJF2.
PRIDEiQ9JJF2.

Expressioni

Tissue specificityi

Isoform 1 is testis specific and is exclusively expressed in spermatids.1 Publication

Gene expression databases

BgeeiQ9JJF2.
CleanExiMM_SPAG4.
ExpressionAtlasiQ9JJF2. baseline and differential.
GenevisibleiQ9JJF2. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with ODF1. May associate with microtubules (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036484.

Structurei

3D structure databases

ProteinModelPortaliQ9JJF2.
SMRiQ9JJF2. Positions 243-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini267 – 427161SUNPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili203 – 24442Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 SUN domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
GeneTreeiENSGT00390000011587.
HOGENOMiHOG000246956.
HOVERGENiHBG079205.
InParanoidiQ9JJF2.
OMAiKHTPNFY.
OrthoDBiEOG7J446H.
TreeFamiTF323915.

Family and domain databases

InterProiIPR027776. SPAG4/SUN4.
IPR012919. SUN_dom.
[Graphical view]
PANTHERiPTHR12911:SF16. PTHR12911:SF16. 1 hit.
PfamiPF07738. Sad1_UNC. 1 hit.
[Graphical view]
PROSITEiPS51469. SUN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJF2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRSPRSGSA ASSHNHTPNF YSENSNSSHS ATSGDSNGRR SAGPELGEPE
60 70 80 90 100
GRRARGSSCG EPALSPGMPG GDTWAGSSRP KLAPRSHNGQ TACGAATVRG
110 120 130 140 150
GASEPSGSSV VLEEQLNLLP ILDLRQEMPT PRVSKSFLSL LFQVLSMVLS
160 170 180 190 200
LAVDGLVCVC REICSIRFLF TAVSLLSIFL AALWWGLLYL IPPLENEPTE
210 220 230 240 250
MLTLSQYHHR VHSQGQQLQQ LQAELNKLHK EVSSVRAAHS ERVAKLVFQR
260 270 280 290 300
LNEDFVRKPD YALSSVGASI DLEKTSSDYE DQNTAYFWNR LSFWNYARPP
310 320 330 340 350
SVILEPDVFP GNCWAFEGDK GQVVIRLPGH VQLSDITLQH PPPTVAHTGG
360 370 380 390 400
ASSAPRDFAV YGLQADDETE VFLGKFIFDV QKSEIQTFHL QNDPPSAFPK
410 420 430 440
VKIQILSNWG HPRFTCLYRV RAHGVRTSEW ADDNATGVTG GPH
Note: Derived from EST data.
Length:443
Mass (Da):48,582
Last modified:July 27, 2011 - v3
Checksum:iF5B8BD2F57E5F200
GO
Isoform 2 (identifier: Q9JJF2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-103: VRGGAS → NRLDLL
     104-443: Missing.

Show »
Length:103
Mass (Da):10,589
Checksum:i06707EEA59ED8972
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811K → E in BAA95039 (Ref. 1) Curated
Sequence conflicti259 – 2591P → T in BAA95039 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 1036VRGGAS → NRLDLL in isoform 2. 1 PublicationVSP_005958
Alternative sequencei104 – 443340Missing in isoform 2. 1 PublicationVSP_005959Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041554 mRNA. Translation: BAA95039.1.
AL833786 Genomic DNA. Translation: CAM46026.1.
CH466551 Genomic DNA. Translation: EDL06167.1.
BB610287 mRNA. No translation available.
BU937622 mRNA. No translation available.
CA466584 mRNA. No translation available.
CCDSiCCDS50777.1. [Q9JJF2-1]
RefSeqiNP_631890.3. NM_139151.4. [Q9JJF2-1]
UniGeneiMm.330713.

Genome annotation databases

EnsembliENSMUST00000038860; ENSMUSP00000036484; ENSMUSG00000038180. [Q9JJF2-1]
GeneIDi245865.
KEGGimmu:245865.
UCSCiuc008nmc.1. mouse. [Q9JJF2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041554 mRNA. Translation: BAA95039.1.
AL833786 Genomic DNA. Translation: CAM46026.1.
CH466551 Genomic DNA. Translation: EDL06167.1.
BB610287 mRNA. No translation available.
BU937622 mRNA. No translation available.
CA466584 mRNA. No translation available.
CCDSiCCDS50777.1. [Q9JJF2-1]
RefSeqiNP_631890.3. NM_139151.4. [Q9JJF2-1]
UniGeneiMm.330713.

3D structure databases

ProteinModelPortaliQ9JJF2.
SMRiQ9JJF2. Positions 243-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036484.

Proteomic databases

PaxDbiQ9JJF2.
PRIDEiQ9JJF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038860; ENSMUSP00000036484; ENSMUSG00000038180. [Q9JJF2-1]
GeneIDi245865.
KEGGimmu:245865.
UCSCiuc008nmc.1. mouse. [Q9JJF2-1]

Organism-specific databases

CTDi6676.
MGIiMGI:2444120. Spag4.

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
GeneTreeiENSGT00390000011587.
HOGENOMiHOG000246956.
HOVERGENiHBG079205.
InParanoidiQ9JJF2.
OMAiKHTPNFY.
OrthoDBiEOG7J446H.
TreeFamiTF323915.

Miscellaneous databases

NextBioi386968.
PROiQ9JJF2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJF2.
CleanExiMM_SPAG4.
ExpressionAtlasiQ9JJF2. baseline and differential.
GenevisibleiQ9JJF2. MM.

Family and domain databases

InterProiIPR027776. SPAG4/SUN4.
IPR012919. SUN_dom.
[Graphical view]
PANTHERiPTHR12911:SF16. PTHR12911:SF16. 1 hit.
PfamiPF07738. Sad1_UNC. 1 hit.
[Graphical view]
PROSITEiPS51469. SUN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-274 (ISOFORM 1).
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-443 (ISOFORM 1).
    Tissue: Testis.
  6. "Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme."
    Shao X., Tarnasky H.A., Lee J.P., Oko R., van der Hoorn F.A.
    Dev. Biol. 211:109-123(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiSPAG4_MOUSE
AccessioniPrimary (citable) accession number: Q9JJF2
Secondary accession number(s): A3KGK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although transmembrane domains are strongly predicted, they may rather represent hydrophobic globular domains associated with microtubules.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.