Q9JJA9 (GRASP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General receptor for phosphoinositides 1-associated scaffold protein
Short name=GRP1-associated scaffold protein

Gene names

Name:	Grasp
ORF Names:	MNCb-4428

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses By similarity.
Subunit structure	Heteromer. Composed of GRASP, PSCD2 and at least one GRM1. Also interacts with GRM2, GRM3 and GRM5 By similarity. Interacts with PSCD3. Ref.1
Subcellular location	Cytoplasm. Cell membrane. Note: Primarily localized to the plasma membrane but also distributed diffusely throughout the cytoplasm. Ref.1
Tissue specificity	Highly expressed in brain, heart and lung, and to a lower extent in embryo, kidney and ovary. Ref.1 Ref.4
Sequence similarities	Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from sequence orthology Ref.4. Source: MGI signal transduction Inferred from physical interaction Ref.1. Source: MGI
Cellular_component	cytoplasm Inferred from sequence orthology Ref.4. Source: MGI nuclear envelope lumen Inferred from sequence orthology Ref.4. Source: MGI plasma membrane Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	General receptor for phosphoinositides 1-associated scaffold protein		PRO_0000087585
Regions
	Domain	100 – 189	90	PDZ
	Region	180 – 257	78	Interaction with PSCD3
	Compositional bias	294 – 323	30	Pro-rich
Amino acid modifications
	Modified residue	93	1	Phosphoserine Ref.5
	Modified residue	236	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	299	1	P → L in BAA95086. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9JJA9 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 52144F07D36B57FE	FASTA	392	42,337
	10 20 30 40 50 60 MTLRRLRKLQ QKEEATAAPD PAGRAPDSEA ARAAPLPSGP PAAAAPPGAP GEELYAALED 70 80 90 100 110 120 YHPAELYRAL AVSGGTLPRR KGSGFRWKNF TQSPEQQRKV LTLEKGDNQT FGFEIQTYGL 130 140 150 160 170 180 HHREEQRVEM VTFVCRVHES SPAQLAGLTP GDTIASVNGL NVEGIRHREI VDIIKASGNV 190 200 210 220 230 240 LRLETLYGTS IRKAELEARL QYLKQTLYEK WGEYRSLMVQ EQRLVHGLVV KDPSIYDTLE 250 260 270 280 290 300 SVRSCLYGAG LLPGSLPFGP LLAAPGSARG GARRAKGDTD DAVYHTCFFG GAEPQALPPP 310 320 330 340 350 360 PPPARALGPS SAETPASVLF PAPRSTLSRS ASVRCAGPGG GGGAPGALWT EAREQALCGA 370 380 390 GLRKTKYRSF RRRLLKFIPG LNRSLEEEES QL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors." Nevrivy D.J., Peterson V.J., Avram D., Ishmael J.E., Hansen S.G., Dowell P., Hruby D.E., Dawson M.I., Leid M. J. Biol. Chem. 275:16827-16836(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH PSCD3, SUBCELLULAR LOCATION.
[2]	"Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method." Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[4]	"Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins." Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S. J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF236099 mRNA. Translation: AAF36997.1. AB041603 mRNA. Translation: BAA95086.1. BC046307 mRNA. Translation: AAH46307.1.
IPI	IPI00123131.
RefSeq	NP_062391.3. NM_019518.3.
UniGene	Mm.276573.
3D structure databases
ProteinModelPortal	Q9JJA9.
SMR	Q9JJA9. Positions 97-191.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000000543.
PTM databases
PhosphoSite	Q9JJA9.
Proteomic databases
PaxDb	Q9JJA9.
PRIDE	Q9JJA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000543; ENSMUSP00000000543; ENSMUSG00000000531.
GeneID	56149.
KEGG	mmu:56149.
UCSC	uc007xsu.1. mouse.
Organism-specific databases
CTD	160622.
MGI	MGI:1860303. Grasp.
Phylogenomic databases
eggNOG	NOG244814.
GeneTree	ENSGT00530000063734.
HOGENOM	HOG000231871.
HOVERGEN	HBG053061.
InParanoid	Q9JJA9.
OMA	EGFRHKE.
OrthoDB	EOG47D9GP.
Gene expression databases
Bgee	Q9JJA9.
Genevestigator	Q9JJA9.
GermOnline	ENSMUSG00000000531. Mus musculus.
Family and domain databases
InterPro	IPR001478. PDZ. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	311928.
SOURCE	Search...

Entry information

Entry name

GRASP_MOUSE

Accession

Primary (citable) accession number: Q9JJA9
Secondary accession number(s): Q9JKL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program