Reviewed, UniProtKB/Swiss-Prot Q9JJ78 (TOPK_MOUSE)

Names and origin

Protein names

Recommended name:
    Lymphokine-activated killer T-cell-originated protein kinase
    EC=2.7.12.2
Alternative name(s):
    T-LAK cell-originated protein kinase
    PDZ-binding kinase

Gene names

Name:	Pbk
Synonyms:	Topk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by phosphorylation By similarity.
Subunit structure	Interacts with DLG1 and TP53 By similarity.
Post-translational modification	Phosphorylated; in a cell-cycle dependent manner at mitosis By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Lymphokine-activated killer T-cell-originated protein kinase		PRO_0000086764
Regions
	Domain	31 – 330	300	Protein kinase
	Nucleotide binding	37 – 45	9	ATP By similarity
Sites
	Active site	166	1	Proton acceptor By similarity
	Binding site	63	1	ATP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	9	1	Phosphothreonine By similarity
	Modified residue	23	1	Phosphothreonine By similarity
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	58	1	Phosphoserine By similarity
	Modified residue	199	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	123	1	N → D in BAB23029. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9JJ78-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D274A4DB44CFEEE0	FASTA	330	36,745
	10 20 30 40 50 60 MEGINNFKTP NKSEKRKSVL CSTPCVNIPA SPFMQKLGFG TGVSVYLMKR SPRGLSHSPW 70 80 90 100 110 120 AVKKISLLCD DHYRTVYQKR LTDEAKILKN LNHPNIIGYR AFTEASDGSL CLAMEYGGEK 130 140 150 160 170 180 SLNDLIEERN KDSGSPFPAA VILRVALHMA RGLKYLHQEK KLLHGDIKSS NVVIKGDFET 190 200 210 220 230 240 IKICDVGVSL PLDENMTVTD PEACYIGTEP WKPKEALEEN GIITDKADVF AFGLTLWEMM 250 260 270 280 290 300 TLCIPHVNLP DDDVDEDATF DESDFDDEAY YAALGTRPSI NMEELDDSYQ KAIELFCVCT 310 320 330 NEDPKDRPSA AHIVEALELD GQCCGLSSKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells." Abe Y., Matsumoto S., Kito K., Ueda N. J. Biol. Chem. 275:21525-21531(2000) [PubMed: 10781613] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetus.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB041882 mRNA. Translation: BAA99578.1. AK003838 mRNA. Translation: BAB23029.1. AK076121 mRNA. Translation: BAC36199.1. BC006754 mRNA. Translation: AAH06754.1. BC020099 mRNA. Translation: AAH20099.1.
IPI	IPI00467350.
RefSeq	NP_075698.1.
UniGene	Mm.24337
3D structure databases
SMR	Q9JJ78. Positions 35-319.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JJ78.
PTM databases
PhosphoSite	Q9JJ78.
Proteomic databases
PRIDE	Q9JJ78.
Genome annotation databases
Ensembl	ENSMUST00000022612; ENSMUSP00000022612; ENSMUSG00000022033; Mus musculus. [Genome view]
GeneID	52033.
KEGG	mmu:52033.
UCSC	uc007ujo.1. mouse.
Organism-specific databases
CTD	52033.
MGI	MGI:1289156. Pbk.
Phylogenomic databases
eggNOG	roNOG13721.
HOGENOM	HBG315301.
HOVERGEN	Q9JJ78.
InParanoid	Q9JJ78.
OMA	GVSVYLM.
OrthoDB	EOG9N8TQS.
PhylomeDB	Q9JJ78.
Enzyme and pathway databases
BRENDA	2.7.12.2. 244.
Gene expression databases
ArrayExpress	Q9JJ78.
Bgee	Q9JJ78.
CleanEx	MM_PBK.
Genevestigator	Q9JJ78.
GermOnline	ENSMUSG00000022033. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	308402.
SOURCE	Search...

Entry information

Entry name

TOPK_MOUSE

Accession

Primary (citable) accession number: Q9JJ78
Secondary accession number(s): Q922V2, Q9D184

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)