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Protein

Lymphokine-activated killer T-cell-originated protein kinase

Gene

Pbk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATPPROSITE-ProRule annotation1
Active sitei166Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 45ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: MGI

GO - Biological processi

  • cellular response to UV Source: MGI
  • mitotic cell cycle Source: InterPro
  • negative regulation of inflammatory response Source: MGI
  • negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • negative regulation of stress-activated MAPK cascade Source: MGI
  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphokine-activated killer T-cell-originated protein kinase (EC:2.7.12.2)
Alternative name(s):
PDZ-binding kinase
T-LAK cell-originated protein kinase
Gene namesi
Name:Pbk
Synonyms:Topk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1289156 Pbk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867641 – 330Lymphokine-activated killer T-cell-originated protein kinaseAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei9PhosphothreonineBy similarity1
Modified residuei23PhosphothreonineBy similarity1
Modified residuei31PhosphoserineBy similarity1
Modified residuei58PhosphoserineCombined sources1
Cross-linki168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylated; in a cell-cycle dependent manner at mitosis.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9JJ78
MaxQBiQ9JJ78
PaxDbiQ9JJ78
PeptideAtlasiQ9JJ78
PRIDEiQ9JJ78

PTM databases

iPTMnetiQ9JJ78
PhosphoSitePlusiQ9JJ78

Expressioni

Gene expression databases

BgeeiENSMUSG00000022033
CleanExiMM_PBK
ExpressionAtlasiQ9JJ78 baseline and differential
GenevisibleiQ9JJ78 MM

Interactioni

Subunit structurei

Interacts with DLG1 and TP53.By similarity

Protein-protein interaction databases

BioGridi206338, 1 interactor
IntActiQ9JJ78, 1 interactor
MINTiQ9JJ78
STRINGi10090.ENSMUSP00000022612

Structurei

3D structure databases

ProteinModelPortaliQ9JJ78
SMRiQ9JJ78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 330Protein kinasePROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0192 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00720000108839
HOGENOMiHOG000294208
HOVERGENiHBG056011
InParanoidiQ9JJ78
KOiK08865
OMAiCTNEDPK
OrthoDBiEOG091G05V7
PhylomeDBiQ9JJ78
TreeFamiTF329763

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
IPR034368 TOPK
PANTHERiPTHR43289:SF3 PTHR43289:SF3, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JJ78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGINNFKTP NKSEKRKSVL CSTPCVNIPA SPFMQKLGFG TGVSVYLMKR
60 70 80 90 100
SPRGLSHSPW AVKKISLLCD DHYRTVYQKR LTDEAKILKN LNHPNIIGYR
110 120 130 140 150
AFTEASDGSL CLAMEYGGEK SLNDLIEERN KDSGSPFPAA VILRVALHMA
160 170 180 190 200
RGLKYLHQEK KLLHGDIKSS NVVIKGDFET IKICDVGVSL PLDENMTVTD
210 220 230 240 250
PEACYIGTEP WKPKEALEEN GIITDKADVF AFGLTLWEMM TLCIPHVNLP
260 270 280 290 300
DDDVDEDATF DESDFDDEAY YAALGTRPSI NMEELDDSYQ KAIELFCVCT
310 320 330
NEDPKDRPSA AHIVEALELD GQCCGLSSKH
Length:330
Mass (Da):36,745
Last modified:October 1, 2000 - v1
Checksum:iD274A4DB44CFEEE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123N → D in BAB23029 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041882 mRNA Translation: BAA99578.1
AK003838 mRNA Translation: BAB23029.1
AK076121 mRNA Translation: BAC36199.1
BC006754 mRNA Translation: AAH06754.1
BC020099 mRNA Translation: AAH20099.1
CCDSiCCDS27215.1
RefSeqiNP_075698.1, NM_023209.2
XP_006519320.1, XM_006519257.3
UniGeneiMm.24337

Genome annotation databases

EnsembliENSMUST00000022612; ENSMUSP00000022612; ENSMUSG00000022033
GeneIDi52033
KEGGimmu:52033
UCSCiuc007ujo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTOPK_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ78
Secondary accession number(s): Q922V2, Q9D184
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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