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Protein

Lymphokine-activated killer T-cell-originated protein kinase

Gene

Pbk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATPPROSITE-ProRule annotation1
Active sitei166Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 45ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: MGI

GO - Biological processi

  • cellular response to UV Source: MGI
  • mitotic cell cycle Source: InterPro
  • negative regulation of inflammatory response Source: MGI
  • negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • negative regulation of stress-activated MAPK cascade Source: MGI
  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphokine-activated killer T-cell-originated protein kinase (EC:2.7.12.2)
Alternative name(s):
PDZ-binding kinase
T-LAK cell-originated protein kinase
Gene namesi
Name:Pbk
Synonyms:Topk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1289156. Pbk.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867641 – 330Lymphokine-activated killer T-cell-originated protein kinaseAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei9PhosphothreonineBy similarity1
Modified residuei23PhosphothreonineBy similarity1
Modified residuei31PhosphoserineBy similarity1
Modified residuei58PhosphoserineCombined sources1
Cross-linki168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylated; in a cell-cycle dependent manner at mitosis.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9JJ78.
MaxQBiQ9JJ78.
PaxDbiQ9JJ78.
PeptideAtlasiQ9JJ78.
PRIDEiQ9JJ78.

PTM databases

iPTMnetiQ9JJ78.
PhosphoSitePlusiQ9JJ78.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022033.
CleanExiMM_PBK.
ExpressionAtlasiQ9JJ78. baseline and differential.
GenevisibleiQ9JJ78. MM.

Interactioni

Subunit structurei

Interacts with DLG1 and TP53.By similarity

Protein-protein interaction databases

BioGridi206338. 1 interactor.
IntActiQ9JJ78. 1 interactor.
MINTiMINT-1341688.
STRINGi10090.ENSMUSP00000022612.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ78.
SMRiQ9JJ78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 330Protein kinasePROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00720000108839.
HOGENOMiHOG000294208.
HOVERGENiHBG056011.
InParanoidiQ9JJ78.
KOiK08865.
OMAiCTNEDPK.
OrthoDBiEOG091G05V7.
PhylomeDBiQ9JJ78.
TreeFamiTF329763.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR034368. TOPK.
PANTHERiPTHR43289:SF3. PTHR43289:SF3. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JJ78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGINNFKTP NKSEKRKSVL CSTPCVNIPA SPFMQKLGFG TGVSVYLMKR
60 70 80 90 100
SPRGLSHSPW AVKKISLLCD DHYRTVYQKR LTDEAKILKN LNHPNIIGYR
110 120 130 140 150
AFTEASDGSL CLAMEYGGEK SLNDLIEERN KDSGSPFPAA VILRVALHMA
160 170 180 190 200
RGLKYLHQEK KLLHGDIKSS NVVIKGDFET IKICDVGVSL PLDENMTVTD
210 220 230 240 250
PEACYIGTEP WKPKEALEEN GIITDKADVF AFGLTLWEMM TLCIPHVNLP
260 270 280 290 300
DDDVDEDATF DESDFDDEAY YAALGTRPSI NMEELDDSYQ KAIELFCVCT
310 320 330
NEDPKDRPSA AHIVEALELD GQCCGLSSKH
Length:330
Mass (Da):36,745
Last modified:October 1, 2000 - v1
Checksum:iD274A4DB44CFEEE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123N → D in BAB23029 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041882 mRNA. Translation: BAA99578.1.
AK003838 mRNA. Translation: BAB23029.1.
AK076121 mRNA. Translation: BAC36199.1.
BC006754 mRNA. Translation: AAH06754.1.
BC020099 mRNA. Translation: AAH20099.1.
CCDSiCCDS27215.1.
RefSeqiNP_075698.1. NM_023209.2.
XP_006519320.1. XM_006519257.3.
UniGeneiMm.24337.

Genome annotation databases

EnsembliENSMUST00000022612; ENSMUSP00000022612; ENSMUSG00000022033.
GeneIDi52033.
KEGGimmu:52033.
UCSCiuc007ujo.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiTOPK_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ78
Secondary accession number(s): Q922V2, Q9D184
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families