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Protein

Kv channel-interacting protein 2

Gene

Kcnip2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:20943905, PubMed:23713033). In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. Essential for the expression of I(To) currents in the heart (PubMed:11747815, PubMed:23713033). Required for normal protein levels of KCND2 in the heart ventricle (PubMed:23713033).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi189 – 2001PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi237 – 2482PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calcium, Metal-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Kv channel-interacting protein 2
Short name:
KChIP2
Alternative name(s):
A-type potassium channel modulatory protein 2
Potassium channel-interacting protein 2
Gene namesi
Name:Kcnip2
Synonyms:Kchip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2135916. Kcnip2.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity

  • Note: Detected on lipid rafts (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • plasma membrane Source: MGI
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice appear healthy and show no signs of cardiac dysmorphogenesis, contractile dysfunction, hypertrophy, or heart failure but are highly susceptible to the induction of polymorphic ventricular tachycardia, including arrhythmia (PubMed:11747815, PubMed:23713033). Myocytes show a complete and selective loss of I(To) current in the heart (PubMed:11747815, PubMed:23713033). This is due to the loss of KCND2 protein in the heart ventricles (PubMed:23713033).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000738221 – 270Kv channel-interacting protein 2Add BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Lipidationi45S-palmitoyl cysteineBy similarity1
Lipidationi46S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated. Palmitoylation enhances association with the plasma membrane.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9JJ69.
PRIDEiQ9JJ69.

PTM databases

iPTMnetiQ9JJ69.
PhosphoSitePlusiQ9JJ69.

Expressioni

Tissue specificityi

Detected in brain (at protein level) (PubMed:23713033). Expressed in brain. Highly expressed in layer IV of the cerebral cortex and in striatum and hippocampus, but expressed at low levels in cerebellum. Also expressed in heart. According to PubMed:11747815 expressed in heart at much higher levels than in brain with a preferential expression in the myocardium.4 Publications

Gene expression databases

BgeeiENSMUSG00000025221.
CleanExiMM_KCNIP2.
ExpressionAtlasiQ9JJ69. baseline and differential.
GenevisibleiQ9JJ69. MM.

Interactioni

Subunit structurei

Component of heteromultimeric potassium channels (PubMed:19713751, PubMed:20943905). Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (PubMed:19713751). The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. At least isoform 2 and isoform 3 can self-associate to form homodimers and homotetramers. Isoform 3 interacts with KCNIP1 in a calcium-dependent manner (By similarity). Interacts with KCND2 (PubMed:20943905). Isoform 1 and isoform 3 interact with KCND3 isoform 1.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125142.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ69.
SMRiQ9JJ69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 137EF-hand 1; degeneratePROSITE-ProRule annotationAdd BLAST57
Domaini140 – 175EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini176 – 211EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini224 – 259EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni257 – 270Interaction with KCND2By similarityAdd BLAST14

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOVERGENiHBG108179.
InParanoidiQ9JJ69.
OMAiMNRCPRR.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR029532. KCNIP2.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF65. PTHR23055:SF65. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJ69-1) [UniParc]FASTAAdd to basket
Also known as: A, KChIP2a, KCHIP2B, KChIP2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGQGRKESL SDSRDLDGSY DQLTGHPPGP SKKALKQRFL KLLPCCGPQA
60 70 80 90 100
LPSVSETLAA PASLRPHRPR PLDPDSVEDE FELSTVCHRP EGLEQLQEQT
110 120 130 140 150
KFTRRELQVL YRGFKNECPS GIVNEENFKQ IYSQFFPQGD SSNYATFLFN
160 170 180 190 200
AFDTNHDGSV SFEDFVAGLS VILRGTIDDR LNWAFNLYDL NKDGCITKEE
210 220 230 240 250
MLDIMKSIYD MMGKYTYPAL REEAPREHVE SFFQKMDRNK DGVVTIEEFI
260 270
ESCQQDENIM RSMQLFDNVI
Length:270
Mass (Da):30,946
Last modified:August 16, 2005 - v2
Checksum:iDB5AC273B2C0F667
GO
Isoform 2 (identifier: Q9JJ69-2) [UniParc]FASTAAdd to basket
Also known as: B, KChIP2b, KCHIP2C

The sequence of this isoform differs from the canonical sequence as follows:
     57-75: TLAAPASLRPHRPRPLDPD → N

Show »
Length:252
Mass (Da):28,997
Checksum:iA54E8D23BDA289ED
GO
Isoform 3 (identifier: Q9JJ69-3) [UniParc]FASTAAdd to basket
Also known as: KCHIP2A, KChIP2b, KChIP2c, KChIP2S

The sequence of this isoform differs from the canonical sequence as follows:
     25-74: Missing.

Show »
Length:220
Mass (Da):25,631
Checksum:iED8BB221099DECCD
GO
Isoform 4 (identifier: Q9JJ69-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MRGQGRKESL...LRPHRPRPLD → MNRCPRRCRSPLGQAARSLYQLVTGSLS

Show »
Length:225
Mass (Da):26,135
Checksum:iC66F919C209473B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12D → E in AAL32044 (PubMed:11747815).Curated1
Sequence conflicti12D → E in AAL32045 (PubMed:11747815).Curated1
Sequence conflicti12D → E in AAL32046 (PubMed:11747815).Curated1
Sequence conflicti31S → T in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti50A → V in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti75D → G in CAC82023 (Ref. 4) Curated1
Sequence conflicti105R → K in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti105R → K in BAA96739 (PubMed:11263977).Curated1
Sequence conflicti143N → T in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti143N → T in BAA96739 (PubMed:11263977).Curated1
Sequence conflicti177I → V in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti177I → V in BAA96739 (PubMed:11263977).Curated1
Sequence conflicti239N → S in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti239N → S in BAA96739 (PubMed:11263977).Curated1
Sequence conflicti248E → G in CAC82024 (Ref. 4) Curated1
Sequence conflicti255Q → K in BAA96738 (PubMed:11263977).Curated1
Sequence conflicti255Q → K in BAA96739 (PubMed:11263977).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0150591 – 73MRGQG…PRPLD → MNRCPRRCRSPLGQAARSLY QLVTGSLS in isoform 4. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_01506025 – 74Missing in isoform 3. 3 PublicationsAdd BLAST50
Alternative sequenceiVSP_01506157 – 75TLAAP…PLDPD → N in isoform 2. 2 PublicationsAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044570 mRNA. Translation: BAA96738.1.
AB044571 mRNA. Translation: BAA96739.1.
AF439339 mRNA. Translation: AAL32044.1.
AF439340 mRNA. Translation: AAL32045.1.
AF439341 mRNA. Translation: AAL32046.1.
AY647241 mRNA. Translation: AAT68467.1.
AJ278535 mRNA. Translation: CAC82023.1.
AJ278536 mRNA. Translation: CAC82024.1.
AJ278537 mRNA. Translation: CAC82026.1.
CCDSiCCDS29868.1. [Q9JJ69-3]
CCDS38004.1. [Q9JJ69-1]
CCDS50453.1. [Q9JJ69-2]
CCDS70955.1. [Q9JJ69-4]
RefSeqiNP_001263287.1. NM_001276358.1. [Q9JJ69-4]
NP_109641.2. NM_030716.3.
NP_663749.1. NM_145703.2.
NP_663750.1. NM_145704.2.
XP_017173804.1. XM_017318315.1.
UniGeneiMm.213204.

Genome annotation databases

EnsembliENSMUST00000086993; ENSMUSP00000084215; ENSMUSG00000025221. [Q9JJ69-4]
GeneIDi80906.
KEGGimmu:80906.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044570 mRNA. Translation: BAA96738.1.
AB044571 mRNA. Translation: BAA96739.1.
AF439339 mRNA. Translation: AAL32044.1.
AF439340 mRNA. Translation: AAL32045.1.
AF439341 mRNA. Translation: AAL32046.1.
AY647241 mRNA. Translation: AAT68467.1.
AJ278535 mRNA. Translation: CAC82023.1.
AJ278536 mRNA. Translation: CAC82024.1.
AJ278537 mRNA. Translation: CAC82026.1.
CCDSiCCDS29868.1. [Q9JJ69-3]
CCDS38004.1. [Q9JJ69-1]
CCDS50453.1. [Q9JJ69-2]
CCDS70955.1. [Q9JJ69-4]
RefSeqiNP_001263287.1. NM_001276358.1. [Q9JJ69-4]
NP_109641.2. NM_030716.3.
NP_663749.1. NM_145703.2.
NP_663750.1. NM_145704.2.
XP_017173804.1. XM_017318315.1.
UniGeneiMm.213204.

3D structure databases

ProteinModelPortaliQ9JJ69.
SMRiQ9JJ69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125142.

PTM databases

iPTMnetiQ9JJ69.
PhosphoSitePlusiQ9JJ69.

Proteomic databases

PaxDbiQ9JJ69.
PRIDEiQ9JJ69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086993; ENSMUSP00000084215; ENSMUSG00000025221. [Q9JJ69-4]
GeneIDi80906.
KEGGimmu:80906.

Organism-specific databases

CTDi30819.
MGIiMGI:2135916. Kcnip2.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOVERGENiHBG108179.
InParanoidiQ9JJ69.
OMAiMNRCPRR.

Enzyme and pathway databases

ReactomeiR-MMU-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

PROiQ9JJ69.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025221.
CleanExiMM_KCNIP2.
ExpressionAtlasiQ9JJ69. baseline and differential.
GenevisibleiQ9JJ69. MM.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR029532. KCNIP2.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF65. PTHR23055:SF65. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCIP2_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ69
Secondary accession number(s): Q6DTJ2
, Q8K1T8, Q8K1T9, Q8K1U0, Q8VHN4, Q8VHN5, Q8VHN6, Q9JJ68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.