Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9JJ69 (KCIP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kv channel-interacting protein 2
Short name=KChIP2
Alternative name(s):
A-type potassium channel modulatory protein 2
Potassium channel-interacting protein 2
Gene names
Name:Kcnip2
Synonyms:Kchip2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCNDV3 trafficking to the cell surface By similarity. Ref.2

Subunit structure

Component of heteromultimeric potassium channels. The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. At least isoform 2 and isoform 3 can self-associate to form homodimers and homotetramers. Isoform 3 interacts with KCNIP1 in a calcium-dependent manner By similarity. Interacts with KCND2. Isoform 1 and isoform 3 interact with KCND3 isoform 1. Ref.5

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in brain. Highly expressed in layer IV of the cerebral cortex and in striatum and hippocampus, but expressed at low levels in cerebellum. Also expressed in heart. According to Ref.2 expressed in heart at much higher levels than in brain. Ref.1 Ref.2 Ref.6

Post-translational modification

Palmitoylated. Palmitoylation enhances association with the plasma membrane By similarity.

Disruption phenotype

Mice are highly susceptible to the induction of cardiac arrhythmias and show a loss of I(To) current in heart. Ref.2

Sequence similarities

Belongs to the recoverin family.

Contains 4 EF-hand domains.

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandCalcium
Potassium
   Molecular functionIonic channel
Potassium channel
Voltage-gated channel
   PTMLipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

potassium channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

voltage-gated ion channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JJ69-1)

Also known as: A; KChIP2a; KCHIP2B; KChIP2L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JJ69-2)

Also known as: B; KChIP2b; KCHIP2C;

The sequence of this isoform differs from the canonical sequence as follows:
     57-75: TLAAPASLRPHRPRPLDPD → N
Isoform 3 (identifier: Q9JJ69-3)

Also known as: KCHIP2A; KChIP2b; KChIP2c; KChIP2S;

The sequence of this isoform differs from the canonical sequence as follows:
     25-74: Missing.
Isoform 4 (identifier: Q9JJ69-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MRGQGRKESL...LRPHRPRPLD → MNRCPRRCRSPLGQAARSLYQLVTGSLS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Kv channel-interacting protein 2
PRO_0000073822

Regions

Domain81 – 13757EF-hand 1; degenerate
Domain140 – 17536EF-hand 2
Domain176 – 21136EF-hand 3
Domain224 – 25936EF-hand 4
Calcium binding189 – 200121 By similarity
Calcium binding237 – 248122 By similarity
Region257 – 27014Interaction with KCND2 By similarity

Amino acid modifications

Modified residue1031Phosphothreonine By similarity
Modified residue1111Phosphotyrosine By similarity
Lipidation451S-palmitoyl cysteine By similarity
Lipidation461S-palmitoyl cysteine By similarity

Natural variations

Alternative sequence1 – 7373MRGQG…PRPLD → MNRCPRRCRSPLGQAARSLY QLVTGSLS in isoform 4.
VSP_015059
Alternative sequence25 – 7450Missing in isoform 3.
VSP_015060
Alternative sequence57 – 7519TLAAP…PLDPD → N in isoform 2.
VSP_015061

Experimental info

Sequence conflict121D → E in AAL32044. Ref.2
Sequence conflict121D → E in AAL32045. Ref.2
Sequence conflict121D → E in AAL32046. Ref.2
Sequence conflict311S → T in BAA96738. Ref.1
Sequence conflict501A → V in BAA96738. Ref.1
Sequence conflict751D → G in CAC82023. Ref.4
Sequence conflict1051R → K in BAA96738. Ref.1
Sequence conflict1051R → K in BAA96739. Ref.1
Sequence conflict1431N → T in BAA96738. Ref.1
Sequence conflict1431N → T in BAA96739. Ref.1
Sequence conflict1771I → V in BAA96738. Ref.1
Sequence conflict1771I → V in BAA96739. Ref.1
Sequence conflict2391N → S in BAA96738. Ref.1
Sequence conflict2391N → S in BAA96739. Ref.1
Sequence conflict2481E → G in CAC82024. Ref.4
Sequence conflict2551Q → K in BAA96738. Ref.1
Sequence conflict2551Q → K in BAA96739. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) (KChIP2a) (KCHIP2B) (KChIP2L) [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: DB5AC273B2C0F667

FASTA27030,946
        10         20         30         40         50         60 
MRGQGRKESL SDSRDLDGSY DQLTGHPPGP SKKALKQRFL KLLPCCGPQA LPSVSETLAA 

        70         80         90        100        110        120 
PASLRPHRPR PLDPDSVEDE FELSTVCHRP EGLEQLQEQT KFTRRELQVL YRGFKNECPS 

       130        140        150        160        170        180 
GIVNEENFKQ IYSQFFPQGD SSNYATFLFN AFDTNHDGSV SFEDFVAGLS VILRGTIDDR 

       190        200        210        220        230        240 
LNWAFNLYDL NKDGCITKEE MLDIMKSIYD MMGKYTYPAL REEAPREHVE SFFQKMDRNK 

       250        260        270 
DGVVTIEEFI ESCQQDENIM RSMQLFDNVI

« Hide

Isoform 2 (B) (KChIP2b) (KCHIP2C) [UniParc].

Checksum: A54E8D23BDA289ED
Show »

FASTA25228,997
Isoform 3 (KCHIP2A) (KChIP2b) (KChIP2c) (KChIP2S) [UniParc].

Checksum: ED8BB221099DECCD
Show »

FASTA22025,631
Isoform 4 [UniParc].

Checksum: C66F919C209473B3
Show »

FASTA22526,135

References

[1]"Molecular cloning and expression of the novel splice variants of K(+) channel-interacting protein 2."
Ohya S., Morohashi Y., Muraki K., Tomita T., Watanabe M., Iwatsubo T., Imaizumi Y.
Biochem. Biophys. Res. Commun. 282:96-102(2001) [PubMed: 11263977] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY.
Tissue: Aorta, Brain, Heart and Stomach.
[2]"A defect in the Kv channel-interacting protein 2 (KChIP2) gene leads to a complete loss of I(to) and confers susceptibility to ventricular tachycardia."
Kuo H.-C., Cheng C.-F., Clark R.B., Lin J.J.C., Lin J.L.C., Hoshijima M., Nguyen-Tran V.T.B., Gu Y., Ikeda Y., Chu P.-H., Ross J. Jr., Giles W.R., Chien K.R.
Cell 107:801-813(2001) [PubMed: 11747815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: BALB/c.
[3]Xia K.U., Fang H.Y., Zhong X.Y., Xia J.H., Zhang Z.H.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[4]Franz O., Soloviev M., Roeper J.
Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-270 (ISOFORMS 1; 2 AND 3).
[5]"Role of heteromultimers in the generation of myocardial transient outward K+ currents."
Guo W., Li H., Aimond F., Johns D.C., Rhodes K.J., Trimmer J.S., Nerbonne J.M.
Circ. Res. 90:586-593(2002) [PubMed: 11909823] [Abstract]
Cited for: INTERACTION WITH KCND2 AND KCND3.
[6]"Differential distribution of KChIPs mRNAs in adult mouse brain."
Xiong H., Kovacs I., Zhang Z.
Brain Res. Mol. Brain Res. 128:103-111(2004) [PubMed: 15363885] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB044570 mRNA. Translation: BAA96738.1.
AB044571 mRNA. Translation: BAA96739.1.
AF439339 mRNA. Translation: AAL32044.1.
AF439340 mRNA. Translation: AAL32045.1.
AF439341 mRNA. Translation: AAL32046.1.
AY647241 mRNA. Translation: AAT68467.1.
AJ278535 mRNA. Translation: CAC82023.1.
AJ278536 mRNA. Translation: CAC82024.1.
AJ278537 mRNA. Translation: CAC82026.1.
IPIIPI00261304.
IPI00399896.
IPI00402825.
IPI00457555.
RefSeqNP_109641.2. NM_030716.2.
NP_663749.1. NM_145703.1.
NP_663750.1. NM_145704.1.
UniGeneMm.213204.

3D structure databases

ProteinModelPortalQ9JJ69.
SMRQ9JJ69. Positions 27-60, 90-270.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9JJ69.

PTM databases

PhosphoSiteQ9JJ69.

Proteomic databases

PRIDEQ9JJ69.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000086993; ENSMUSP00000084215; ENSMUSG00000025221.
GeneID80906.
KEGGmmu:80906.

Organism-specific databases

CTD30819.
MGIMGI:2135916. Kcnip2.

Phylogenomic databases

GeneTreeENSGT00560000076973.
HOVERGENHBG108179.
InParanoidQ9JJ69.
OMAILRGTTD.
OrthoDBEOG4K6G52.
PhylomeDBQ9JJ69.

Gene expression databases

ArrayExpressQ9JJ69.
BgeeQ9JJ69.
CleanExMM_KCNIP2.
GenevestigatorQ9JJ69.
GermOnlineENSMUSG00000025221. Mus musculus.

Family and domain databases

InterProIPR018248. EF-hand.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
IPR001125. Recoverin.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PfamPF00036. efhand. 1 hit.
[Graphical view]
PRINTSPR00450. RECOVERIN.
SMARTSM00054. EFh. 3 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio350258.
SOURCESearch...

Entry information

Entry nameKCIP2_MOUSE
AccessionPrimary (citable) accession number: Q9JJ69
Secondary accession number(s): Q6DTJ2 expand/collapse secondary AC list , Q8K1T8, Q8K1T9, Q8K1U0, Q8VHN4, Q8VHN5, Q8VHN6, Q9JJ68
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: September 21, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents