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Q9JJ66 (CDC20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division cycle protein 20 homolog

Short name=mmCdc20
Alternative name(s):
p55CDC
Gene names
Name:Cdc20
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Ref.7

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL and MAD2L1. Interacts with CDK5RAP2 By similarity. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with NEK2 By similarity. Ref.6 Ref.7

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole By similarity.

Post-translational modification

Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2 By similarity.

Dephosphorylated by CTDP1 By similarity.

Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex By similarity.

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.

Contains 7 WD repeats.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Differentiation
Mitosis
Neurogenesis
Ubl conjugation pathway
   Cellular componentCytoplasm
Cytoskeleton
   DomainRepeat
WD repeat
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of anaphase-promoting complex activity

Inferred from sequence or structural similarity. Source: UniProtKB

anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: Ensembl

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of synapse maturation

Inferred from direct assay Ref.7. Source: UniProtKB

positive regulation of synaptic plasticity

Inferred from direct assay Ref.7. Source: UniProtKB

protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

regulation of dendrite development

Inferred from electronic annotation. Source: Ensembl

regulation of meiosis

Inferred from genetic interaction PubMed 16456547. Source: MGI

   Cellular_componentanaphase-promoting complex

Inferred from electronic annotation. Source: Ensembl

centrosome

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

spindle pole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Fbxo43Q8CDI22EBI-2551389,EBI-8060482
Sirt2Q8VDQ82EBI-2551389,EBI-911012

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Cell division cycle protein 20 homolog
PRO_0000050901

Regions

Repeat182 – 22140WD 1
Repeat224 – 26340WD 2
Repeat266 – 30338WD 3
Repeat307 – 34640WD 4
Repeat353 – 39543WD 5
Repeat397 – 43842WD 6
Repeat441 – 48040WD 7

Amino acid modifications

Modified residue411Phosphoserine By similarity
Modified residue701Phosphothreonine By similarity
Modified residue721Phosphoserine By similarity
Modified residue921Phosphoserine By similarity
Modified residue1061Phosphothreonine By similarity
Modified residue1531Phosphoserine By similarity
Modified residue1571Phosphothreonine By similarity
Modified residue1611Phosphoserine By similarity
Cross-link485Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link490Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict851A → G in BAA97451. Ref.1
Sequence conflict3971D → E in BAC36132. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9JJ66 [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: 81E0781BA87BA79E

FASTA49954,816
        10         20         30         40         50         60 
MAQFVFESDL HSLLQLDAPI PNAPVARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP 

        70         80         90        100        110        120 
GKSSSKVQTT PSKPGGDRFI PQRSASQMEV ASFLLSKENQ PEDRGTPTKK EHQKAWSLNL 

       130        140        150        160        170        180 
NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE 

       190        200        210        220        230        240 
IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY 

       250        260        270        280        290        300 
LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH 

       310        320        330        340        350        360 
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWAPLQT FTQHQGAVKA 

       370        380        390        400        410        420 
VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF 

       430        440        450        460        470        480 
AQNQLVIWKY PTMAKVAELK GHTARVLGLT MSPDGATVAS AAADETLRLW RCFEMDPALR 

       490 
REREKASVAK SSLIHQGIR 

« Hide

References

« Hide 'large scale' references
[1]"Mus musculus CDC20 mRNA, complete cds."
Kuroda M., Oikawa K., Ohbayashi T., Iwata R., Kameta A., Ebine K.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Ssc1/Elovl1 and p55Cdc genes are co-expressed in a tail-to-tail array in proliferating cells."
Asadi A., Jacobsson A.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[5]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 59-66, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[6]"Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20."
Goto M., Eddy E.M.
J. Biol. Chem. 279:42128-42138(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPATC1, IDENTIFICATION IN A COMPLEX WITH CDC27; SPATC1 AND TUBG1.
[7]"A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation."
Yang Y., Kim A.H., Yamada T., Wu B., Bilimoria P.M., Ikeuchi Y., de la Iglesia N., Shen J., Bonni A.
Science 326:575-578(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NEUROD2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB045313 mRNA. Translation: BAA97451.1.
AF312208 mRNA. Translation: AAL25714.1.
AK075998 mRNA. Translation: BAC36109.1.
AK076030 mRNA. Translation: BAC36132.1.
AK083459 mRNA. Translation: BAC38922.1.
AK168650 mRNA. Translation: BAE40507.1.
BC003215 mRNA. Translation: AAH03215.1.
CCDSCCDS18551.1.
RefSeqNP_075712.2. NM_023223.2.
UniGeneMm.289747.

3D structure databases

ProteinModelPortalQ9JJ66.
SMRQ9JJ66. Positions 165-478.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid223749. 28 interactions.
IntActQ9JJ66. 21 interactions.
MINTMINT-2834618.

PTM databases

PhosphoSiteQ9JJ66.

Proteomic databases

PaxDbQ9JJ66.
PRIDEQ9JJ66.

Protocols and materials databases

DNASU107995.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006565; ENSMUSP00000006565; ENSMUSG00000006398.
GeneID107995.
KEGGmmu:107995.
UCSCuc008ukc.2. mouse.

Organism-specific databases

CTD991.
MGIMGI:1859866. Cdc20.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00750000117562.
HOGENOMHOG000195514.
HOVERGENHBG001024.
InParanoidQ9JJ66.
KOK03363.
OMAVSSLCWN.
OrthoDBEOG76X602.
PhylomeDBQ9JJ66.
TreeFamTF101065.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

BgeeQ9JJ66.
CleanExMM_CDC20.
GenevestigatorQ9JJ66.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 5 hits.
[Graphical view]
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio359849.
PROQ9JJ66.
SOURCESearch...

Entry information

Entry nameCDC20_MOUSE
AccessionPrimary (citable) accession number: Q9JJ66
Secondary accession number(s): Q3TGP1, Q8BPG4, Q99LK3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot