Q9JJ66 (CDC20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division cycle protein 20 homolog
Short name=mmCdc20

Alternative name(s):
p55CDC

Gene names

Name:

Cdc20

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL and MAD2L1. Interacts with CDK5RAP2 By similarity. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with NEK2 By similarity. Ref.6 Ref.7
Subcellular location	Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity.
Post-translational modification	Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2 By similarity. Dephosphorylated by CTDP1 By similarity. Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C completo bind the APC/C complex By similarity.
Sequence similarities	Belongs to the WD repeat CDC20/Fizzy family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis Ubl conjugation pathway
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat WD repeat
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity Inferred from sequence or structural similarity. Source: UniProtKB anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of synapse maturation Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of synaptic plasticity Inferred from direct assay Ref.7. Source: UniProtKB protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of dendrite development Inferred from electronic annotation. Source: Compara regulation of meiosis Inferred from genetic interaction PubMed 16456547. Source: MGI
Cellular_component	anaphase-promoting complex Inferred from electronic annotation. Source: Compara centrosome Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Sirt2	Q8VDQ8	2	EBI-2551389,EBI-911012

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Cell division cycle protein 20 homolog		PRO_0000050901
Regions
	Repeat	182 – 221	40	WD 1
	Repeat	224 – 263	40	WD 2
	Repeat	266 – 303	38	WD 3
	Repeat	307 – 346	40	WD 4
	Repeat	353 – 395	43	WD 5
	Repeat	397 – 438	42	WD 6
	Repeat	441 – 480	40	WD 7
Amino acid modifications
	Modified residue	41	1	Phosphoserine By similarity
	Modified residue	70	1	Phosphothreonine By similarity
	Modified residue	72	1	Phosphoserine By similarity
	Modified residue	92	1	Phosphoserine By similarity
	Modified residue	106	1	Phosphothreonine By similarity
	Modified residue	153	1	Phosphoserine By similarity
	Modified residue	157	1	Phosphothreonine By similarity
	Modified residue	161	1	Phosphoserine By similarity
	Cross-link	485		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	490		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	85	1	A → G in BAA97451. Ref.1
	Sequence conflict	397	1	D → E in BAC36132. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9JJ66 [UniParc]. Last modified October 3, 2003. Version 2. Checksum: 81E0781BA87BA79E	FASTA	499	54,816
	10 20 30 40 50 60 MAQFVFESDL HSLLQLDAPI PNAPVARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP 70 80 90 100 110 120 GKSSSKVQTT PSKPGGDRFI PQRSASQMEV ASFLLSKENQ PEDRGTPTKK EHQKAWSLNL 130 140 150 160 170 180 NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE 190 200 210 220 230 240 IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY 250 260 270 280 290 300 LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH 310 320 330 340 350 360 HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWAPLQT FTQHQGAVKA 370 380 390 400 410 420 VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF 430 440 450 460 470 480 AQNQLVIWKY PTMAKVAELK GHTARVLGLT MSPDGATVAS AAADETLRLW RCFEMDPALR 490 REREKASVAK SSLIHQGIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mus musculus CDC20 mRNA, complete cds." Kuroda M., Oikawa K., Ohbayashi T., Iwata R., Kameta A., Ebine K. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Ssc1/Elovl1 and p55Cdc genes are co-expressed in a tail-to-tail array in proliferating cells." Asadi A., Jacobsson A. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[5]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 59-66, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[6]	"Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20." Goto M., Eddy E.M. J. Biol. Chem. 279:42128-42138(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SPATC1, IDENTIFICATION IN A COMPLEX WITH CDC27; SPATC1 AND TUBG1.
[7]	"A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation." Yang Y., Kim A.H., Yamada T., Wu B., Bilimoria P.M., Ikeuchi Y., de la Iglesia N., Shen J., Bonni A. Science 326:575-578(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NEUROD2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB045313 mRNA. Translation: BAA97451.1. AF312208 mRNA. Translation: AAL25714.1. AK075998 mRNA. Translation: BAC36109.1. AK076030 mRNA. Translation: BAC36132.1. AK083459 mRNA. Translation: BAC38922.1. AK168650 mRNA. Translation: BAE40507.1. BC003215 mRNA. Translation: AAH03215.1.
IPI	IPI00320406.
RefSeq	NP_075712.2. NM_023223.2.
UniGene	Mm.289747.
3D structure databases
ProteinModelPortal	Q9JJ66.
SMR	Q9JJ66. Positions 165-478.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JJ66. 19 interactions.
MINT	MINT-2834618.
PTM databases
PhosphoSite	Q9JJ66.
Proteomic databases
PaxDb	Q9JJ66.
PRIDE	Q9JJ66.
Protocols and materials databases
DNASU	107995.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006565; ENSMUSP00000006565; ENSMUSG00000006398.
GeneID	107995.
KEGG	mmu:107995.
Organism-specific databases
CTD	991.
MGI	MGI:1859866. Cdc20.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00580000081599.
HOGENOM	HOG000195514.
HOVERGEN	HBG001024.
InParanoid	Q9JJ66.
KO	K03363.
OMA	RTPGKSN.
OrthoDB	EOG4CC414.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9JJ66.
Bgee	Q9JJ66.
CleanEx	MM_CDC20.
Genevestigator	Q9JJ66.
GermOnline	ENSMUSG00000006398. Mus musculus.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 5 hits. [Graphical view]
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	359849.
SOURCE	Search...

Entry information

Entry name

CDC20_MOUSE

Accession

Primary (citable) accession number: Q9JJ66
Secondary accession number(s): Q3TGP1, Q8BPG4, Q99LK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	October 3, 2003
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program