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Q9JJ66

- CDC20_MOUSE

UniProt

Q9JJ66 - CDC20_MOUSE

Protein

Cell division cycle protein 20 homolog

Gene

Cdc20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (03 Oct 2003)
      Previous versions | rss
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    Functioni

    Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. activation of anaphase-promoting complex activity Source: UniProtKB
    2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
    3. mitotic nuclear division Source: UniProtKB-KW
    4. positive regulation of cell proliferation Source: Ensembl
    5. positive regulation of synapse maturation Source: UniProtKB
    6. positive regulation of synaptic plasticity Source: UniProtKB
    7. protein ubiquitination Source: UniProtKB-UniPathway
    8. regulation of dendrite development Source: Ensembl
    9. regulation of meiosis Source: MGI

    Keywords - Biological processi

    Cell cycle, Cell division, Differentiation, Mitosis, Neurogenesis, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_203517. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_204224. Phosphorylation of Emi1.
    REACT_207679. Separation of Sister Chromatids.
    REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division cycle protein 20 homolog
    Short name:
    mmCdc20
    Alternative name(s):
    p55CDC
    Gene namesi
    Name:Cdc20
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1859866. Cdc20.

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: Ensembl
    2. centrosome Source: Ensembl
    3. perinuclear region of cytoplasm Source: Ensembl
    4. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Cell division cycle protein 20 homologPRO_0000050901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411PhosphoserineBy similarity
    Modified residuei66 – 661N6-acetyllysineBy similarity
    Modified residuei70 – 701PhosphothreonineBy similarity
    Modified residuei72 – 721PhosphoserineBy similarity
    Modified residuei92 – 921PhosphoserineBy similarity
    Modified residuei106 – 1061PhosphothreonineBy similarity
    Modified residuei153 – 1531PhosphoserineBy similarity
    Modified residuei157 – 1571PhosphothreonineBy similarity
    Modified residuei161 – 1611PhosphoserineBy similarity
    Cross-linki485 – 485Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki490 – 490Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Post-translational modificationi

    Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).1 Publication
    Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2 By similarity.By similarity
    Dephosphorylated by CTDP1.By similarity
    Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex By similarity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ9JJ66.
    PRIDEiQ9JJ66.

    PTM databases

    PhosphoSiteiQ9JJ66.

    Expressioni

    Gene expression databases

    BgeeiQ9JJ66.
    CleanExiMM_CDC20.
    GenevestigatoriQ9JJ66.

    Interactioni

    Subunit structurei

    Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL and MAD2L1. Interacts with CDK5RAP2. Interacts with NEK2 By similarity. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2, SIRT2 and SPATC1.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Fbxo43Q8CDI22EBI-2551389,EBI-8060482
    Sirt2Q8VDQ82EBI-2551389,EBI-911012

    Protein-protein interaction databases

    BioGridi223749. 29 interactions.
    IntActiQ9JJ66. 21 interactions.
    MINTiMINT-2834618.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JJ66.
    SMRiQ9JJ66. Positions 165-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati182 – 22140WD 1Add
    BLAST
    Repeati224 – 26340WD 2Add
    BLAST
    Repeati266 – 30338WD 3Add
    BLAST
    Repeati307 – 34640WD 4Add
    BLAST
    Repeati353 – 39543WD 5Add
    BLAST
    Repeati397 – 43842WD 6Add
    BLAST
    Repeati441 – 48040WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat CDC20/Fizzy family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00750000117562.
    HOGENOMiHOG000195514.
    HOVERGENiHBG001024.
    InParanoidiQ9JJ66.
    KOiK03363.
    OMAiVSSLCWN.
    OrthoDBiEOG76X602.
    PhylomeDBiQ9JJ66.
    TreeFamiTF101065.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 5 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 3 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JJ66-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQFVFESDL HSLLQLDAPI PNAPVARWQR KAKEATGPAP SPMRAANRSH    50
    SAGRTPGRTP GKSSSKVQTT PSKPGGDRFI PQRSASQMEV ASFLLSKENQ 100
    PEDRGTPTKK EHQKAWSLNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV 150
    LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV 200
    ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV 250
    QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH 300
    HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWAPLQT 350
    FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS 400
    QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLGLT 450
    MSPDGATVAS AAADETLRLW RCFEMDPALR REREKASVAK SSLIHQGIR 499
    Length:499
    Mass (Da):54,816
    Last modified:October 3, 2003 - v2
    Checksum:i81E0781BA87BA79E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851A → G in BAA97451. 1 PublicationCurated
    Sequence conflicti397 – 3971D → E in BAC36132. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB045313 mRNA. Translation: BAA97451.1.
    AF312208 mRNA. Translation: AAL25714.1.
    AK075998 mRNA. Translation: BAC36109.1.
    AK076030 mRNA. Translation: BAC36132.1.
    AK083459 mRNA. Translation: BAC38922.1.
    AK168650 mRNA. Translation: BAE40507.1.
    BC003215 mRNA. Translation: AAH03215.1.
    CCDSiCCDS18551.1.
    RefSeqiNP_075712.2. NM_023223.2.
    UniGeneiMm.289747.

    Genome annotation databases

    EnsembliENSMUST00000006565; ENSMUSP00000006565; ENSMUSG00000006398.
    GeneIDi107995.
    KEGGimmu:107995.
    UCSCiuc008ukc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB045313 mRNA. Translation: BAA97451.1 .
    AF312208 mRNA. Translation: AAL25714.1 .
    AK075998 mRNA. Translation: BAC36109.1 .
    AK076030 mRNA. Translation: BAC36132.1 .
    AK083459 mRNA. Translation: BAC38922.1 .
    AK168650 mRNA. Translation: BAE40507.1 .
    BC003215 mRNA. Translation: AAH03215.1 .
    CCDSi CCDS18551.1.
    RefSeqi NP_075712.2. NM_023223.2.
    UniGenei Mm.289747.

    3D structure databases

    ProteinModelPortali Q9JJ66.
    SMRi Q9JJ66. Positions 165-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223749. 29 interactions.
    IntActi Q9JJ66. 21 interactions.
    MINTi MINT-2834618.

    PTM databases

    PhosphoSitei Q9JJ66.

    Proteomic databases

    PaxDbi Q9JJ66.
    PRIDEi Q9JJ66.

    Protocols and materials databases

    DNASUi 107995.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006565 ; ENSMUSP00000006565 ; ENSMUSG00000006398 .
    GeneIDi 107995.
    KEGGi mmu:107995.
    UCSCi uc008ukc.2. mouse.

    Organism-specific databases

    CTDi 991.
    MGIi MGI:1859866. Cdc20.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00750000117562.
    HOGENOMi HOG000195514.
    HOVERGENi HBG001024.
    InParanoidi Q9JJ66.
    KOi K03363.
    OMAi VSSLCWN.
    OrthoDBi EOG76X602.
    PhylomeDBi Q9JJ66.
    TreeFami TF101065.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_203517. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_204224. Phosphorylation of Emi1.
    REACT_207679. Separation of Sister Chromatids.
    REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

    Miscellaneous databases

    NextBioi 359849.
    PROi Q9JJ66.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JJ66.
    CleanExi MM_CDC20.
    Genevestigatori Q9JJ66.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 5 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 3 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mus musculus CDC20 mRNA, complete cds."
      Kuroda M., Oikawa K., Ohbayashi T., Iwata R., Kameta A., Ebine K.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Ssc1/Elovl1 and p55Cdc genes are co-expressed in a tail-to-tail array in proliferating cells."
      Asadi A., Jacobsson A.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 59-66, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. "Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20."
      Goto M., Eddy E.M.
      J. Biol. Chem. 279:42128-42138(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPATC1, IDENTIFICATION IN A COMPLEX WITH CDC27; SPATC1 AND TUBG1.
    7. "A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation."
      Yang Y., Kim A.H., Yamada T., Wu B., Bilimoria P.M., Ikeuchi Y., de la Iglesia N., Shen J., Bonni A.
      Science 326:575-578(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NEUROD2.
    8. "SIRT2 maintains genome integrity and suppresses tumorigenesis through regulating APC/C activity."
      Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X.
      Cancer Cell 20:487-499(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION, DEACETYLATION BY SIRT2, INTERACTION WITH SIRT2.

    Entry informationi

    Entry nameiCDC20_MOUSE
    AccessioniPrimary (citable) accession number: Q9JJ66
    Secondary accession number(s): Q3TGP1, Q8BPG4, Q99LK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: October 3, 2003
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3