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Protein

Polypeptide N-acetylgalactosaminyltransferase 16

Gene

Galnt16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631SubstrateBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Metal bindingi211 – 2111ManganeseBy similarity
Binding sitei212 – 2121SubstrateBy similarity
Metal bindingi213 – 2131ManganeseBy similarity
Binding sitei317 – 3171SubstrateBy similarity
Metal bindingi345 – 3451ManganeseBy similarity
Binding sitei348 – 3481SubstrateBy similarity
Binding sitei351 – 3511SubstrateBy similarity
Binding sitei353 – 3531SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 16 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 16
Short name:
GalNAc-T16
Polypeptide GalNAc transferase-like protein 1
Short name:
GalNAc-T-like protein 1
Short name:
pp-GaNTase-like protein 1
Polypeptide N-acetylgalactosaminyltransferase-like protein 1
Protein-UDP acetylgalactosaminyltransferase-like protein 1
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 1
Gene namesi
Name:Galnt16
Synonyms:Galntl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1917754. Galnt16.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2620Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini27 – 558532LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Polypeptide N-acetylgalactosaminyltransferase 16PRO_0000059136Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi113 ↔ 340PROSITE-ProRule annotation
Disulfide bondi331 ↔ 409PROSITE-ProRule annotation
Disulfide bondi441 ↔ 460PROSITE-ProRule annotation
Disulfide bondi486 ↔ 506PROSITE-ProRule annotation
Disulfide bondi530 ↔ 543PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9JJ61.
PaxDbiQ9JJ61.
PRIDEiQ9JJ61.

PTM databases

PhosphoSiteiQ9JJ61.

Expressioni

Tissue specificityi

In the CNS, it is predominantly expressed in several distinct hypothalamic, thalamic and amygdaloid nuclei. The most abundant level of expression is in the paraventricular, ventromedial and arcuate nuclei of the hypothalamus, the anterodorsal and parafascicular nuclei of the thalamus and the central, basomedial and medial nuclei of the amygdala. Also expressed in cerebral cortex, lateral septum, habenula and hippocampus.1 Publication

Gene expression databases

BgeeiQ9JJ61.
ExpressionAtlasiQ9JJ61. baseline and differential.
GenevisibleiQ9JJ61. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021558.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ61.
SMRiQ9JJ61. Positions 68-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini428 – 555128Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 227106Catalytic subdomain AAdd
BLAST
Regioni286 – 34863Catalytic subdomain BAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00760000118828.
HOVERGENiHBG051699.
InParanoidiQ9JJ61.
KOiK00710.
OMAiPEDCQLL.
OrthoDBiEOG7J9VP2.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJ61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIRANAIA ILTVAWILGT FYYLWQDNRA HAASSSGRGA QRAGGRPEQL
60 70 80 90 100
REDRTIPLIV TGTPSKGFDE KAYLSAKQLK PGEDPYRQHA FNQLESDKLS
110 120 130 140 150
SDRPIRDTRH YSCPSLSYSS DLPATSVIIT FHNEARSTLL RTVKSVLNRT
160 170 180 190 200
PASLIQEIIL VDDFSSDPED CLLLTRIPKV KCLRNDKREG LIRSRVRGAD
210 220 230 240 250
VAGATVLTFL DSHCEVNVEW LQPMLQRVME DHTRVVSPII DVISLDNFAY
260 270 280 290 300
LAASADLRGG FDWSLHFKWE QIPLEQKMTR TDPTKPIRTP VIAGGIFVID
310 320 330 340 350
KSWFNHLGKY DAQMDIWGGE NFELSFRVWM CGGSLEIVPC SRVGHVFRKR
360 370 380 390 400
HPYNFPEGNA LTYIRNTKRT AEVWMDEYKQ YYYEARPSAI GKAFGSVATR
410 420 430 440 450
IEQRKKMDCK SFRWYLENVY PELTVPVKEV LPGVIKQGVN CLESQGQNTA
460 470 480 490 500
GDLLLGMGIC RGSAKSPPPA QAWLFSDHLI QQQGKCLAAT STLMSSPGSP
510 520 530 540 550
VILQTCNPKE GKQKWRRKGS FIQHSVSGLC LETKPAQLVT SKCQTDAQAQ

QWQLLPHT
Length:558
Mass (Da):62,875
Last modified:July 27, 2011 - v2
Checksum:i1B59DBE8EDDD5C63
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711K → E in BAA97985 (Ref. 1) Curated
Sequence conflicti198 – 1981G → R in BAA97985 (Ref. 1) Curated
Sequence conflicti283 – 2831P → L in BAA97985 (Ref. 1) Curated
Sequence conflicti518 – 5181K → R in BAA97985 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045325 mRNA. Translation: BAA97985.1.
AB175683 mRNA. Translation: BAD52071.1.
AK138883 mRNA. Translation: BAE23810.1.
BC110634 mRNA. Translation: AAI10635.1.
BC125015 mRNA. Translation: AAI25016.1.
CCDSiCCDS36482.1.
RefSeqiNP_001074890.1. NM_001081421.1.
UniGeneiMm.308296.

Genome annotation databases

EnsembliENSMUST00000021558; ENSMUSP00000021558; ENSMUSG00000021130.
GeneIDi108760.
KEGGimmu:108760.
UCSCiuc007oaw.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Putative polypeptide N-acetylgalactosaminyltransferase-like protein 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045325 mRNA. Translation: BAA97985.1.
AB175683 mRNA. Translation: BAD52071.1.
AK138883 mRNA. Translation: BAE23810.1.
BC110634 mRNA. Translation: AAI10635.1.
BC125015 mRNA. Translation: AAI25016.1.
CCDSiCCDS36482.1.
RefSeqiNP_001074890.1. NM_001081421.1.
UniGeneiMm.308296.

3D structure databases

ProteinModelPortaliQ9JJ61.
SMRiQ9JJ61. Positions 68-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021558.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSiteiQ9JJ61.

Proteomic databases

MaxQBiQ9JJ61.
PaxDbiQ9JJ61.
PRIDEiQ9JJ61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021558; ENSMUSP00000021558; ENSMUSG00000021130.
GeneIDi108760.
KEGGimmu:108760.
UCSCiuc007oaw.1. mouse.

Organism-specific databases

CTDi57452.
MGIiMGI:1917754. Galnt16.

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00760000118828.
HOVERGENiHBG051699.
InParanoidiQ9JJ61.
KOiK00710.
OMAiPEDCQLL.
OrthoDBiEOG7J9VP2.
TreeFamiTF313267.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi361295.
PROiQ9JJ61.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JJ61.
ExpressionAtlasiQ9JJ61. baseline and differential.
GenevisibleiQ9JJ61. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, mpp-GalNAc-T16."
    Zhang Y., Kwon Y., Kikuchi N., Narimatsu H.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "A new UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase mRNA exhibits predominant expression in the hypothalamus, thalamus and amygdala of mouse forebrain."
    Nelson P.A., Sutcliffe J.G., Thomas E.A.
    Gene Expr. Patterns 1:95-99(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGLT16_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ61
Secondary accession number(s): Q60GT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally termed Galnt10/pp-GaNTase 10.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.