Q9JJ59 (ABCB9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-binding cassette sub-family B member 9

Alternative name(s):
ATP-binding cassette transporter 9
Short name=ABC transporter 9 protein
Short name=mABCB9
TAP-like protein
Short name=TAPL

Gene names

Name:	Abcb9
Synonyms:	Kiaa1520

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Lysosome membrane; Multi-pass membrane protein By similarity. Note: May be located in membrane rafts By similarity.
Tissue specificity	Highly expressed in testis, particularly in the Sertoli cells of the seminiferous tubules, and at moderate levels in brain and spinal cord. Ref.2
Domain	The N-terminal region comprising the first four transmembrane domains is required for lysosomal localization but not for homodimerization or peptide transport By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.
Sequence caution	The sequence BAC41480.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Peptide transport Protein transport Transport
Cellular component	Lysosome Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of T cell mediated cytotoxicity Inferred from Biological aspect of Ancestor. Source: RefGenome protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	TAP complex Inferred from Biological aspect of Ancestor. Source: RefGenome lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from sequence orthology Ref.2. Source: MGI plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MHC class I protein binding Inferred from Biological aspect of Ancestor. Source: RefGenome TAP1 binding Inferred from Biological aspect of Ancestor. Source: RefGenome TAP2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome oligopeptide-transporting ATPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome peptide antigen binding Inferred from Biological aspect of Ancestor. Source: RefGenome tapasin binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JJ59-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JJ59-2)

The sequence of this isoform differs from the canonical sequence as follows:
     631-636: ETGEKG → GTRRRL
     637-762: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	ATP-binding cassette sub-family B member 9		PRO_0000000253
Regions
	Transmembrane	7 – 27	21	Helical; Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Transmembrane	315 – 335	21	Helical; Potential
	Transmembrane	412 – 432	21	Helical; Potential
	Domain	184 – 467	284	ABC transmembrane type-1
	Domain	500 – 736	237	ABC transporter
	Nucleotide binding	535 – 542	8	ATP Potential
	Compositional bias	380 – 385	6	Poly-Glu
Natural variations
	Alternative sequence	631 – 636	6	ETGEKG → GTRRRL in isoform 2.		VSP_000032
	Alternative sequence	637 – 762	126	Missing in isoform 2.		VSP_000033
Experimental info
	Sequence conflict	67	1	G → R in AAF89994. Ref.2
	Sequence conflict	513	1	P → A in AK020749. Ref.4
	Sequence conflict	524	1	S → N in AK020749. Ref.4
	Sequence conflict	550	1	N → S in AAF89994. Ref.2
	Sequence conflict	605	1	F → L in AK020749. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A03C41760974DC9F	FASTA	762	83,963
	10 20 30 40 50 60 MRLWKAVVVT LAFVSTDVGV TTAIYAFSHL DRSLLEDIRH FNIFDSVLDL WAACLYRSCL 70 80 90 100 110 120 LLGATIGVAK NSALGPRRLR ASWLVITLVC LFVGIYAMAK LLLFSEVRRP IRDPWFWALF 130 140 150 160 170 180 VWTYISLAAS FLLWGLLATV RPDAEALEPG NEGFHGEGGA PAEQASGATL QKLLSYTKPD 190 200 210 220 230 240 VAFLVAASFF LIVAALGETF LPYYTGRAID SIVIQKSMDQ FTTAVVVVCL LAIGSSLAAG 250 260 270 280 290 300 IRGGIFTLVF ARLNIRLRNC LFRSLVSQET SFFDENRTGD LISRLTSDTT MVSDLVSQNI 310 320 330 340 350 360 NIFLRNTVKV TGVVVFMFSL SWQLSLVTFM GFPIIMMVSN IYGKYYKRLS KEVQSALARA 370 380 390 400 410 420 STTAEETISA MKTVRSFANE EEEAEVFLRK LQQVYKLNRK EAAAYMSYVW GSGLTLLVVQ 430 440 450 460 470 480 VSILYYGGHL VISGQMSSGN LIAFIIYEFV LGDCMESVGS VYSGLMQGVG AAEKVFEFID 490 500 510 520 530 540 RQPTMVHDGS LAPDHLEGRV DFENVTFTYR TRPHTQVLQN VSFSLSPGKV TALVGPSGSG 550 560 570 580 590 600 KSSCVNILEN FYPLQGGRVL LDGKPIGAYD HKYLHRVISL VSQEPVLFAR SITDNISYGL 610 620 630 640 650 660 PTVPFEMVVE AAQKANAHGF IMELQDGYST ETGEKGAQLS GGQKQRVAMA RALVRNPPVL 670 680 690 700 710 720 ILDEATSALD AESEYLIQQA IHGNLQRHTV LIIAHRLSTV ERAHLIVVLD KGRVVQQGTH 730 740 750 760 QQLLAQGGLY AKLVQRQMLG LEHPLDYTAS HKEPPSNTEH KA
	Isoform 2 [UniParc]. Checksum: 0740982E661B370E	FASTA	636	70,216
	10 20 30 40 50 60 MRLWKAVVVT LAFVSTDVGV TTAIYAFSHL DRSLLEDIRH FNIFDSVLDL WAACLYRSCL 70 80 90 100 110 120 LLGATIGVAK NSALGPRRLR ASWLVITLVC LFVGIYAMAK LLLFSEVRRP IRDPWFWALF 130 140 150 160 170 180 VWTYISLAAS FLLWGLLATV RPDAEALEPG NEGFHGEGGA PAEQASGATL QKLLSYTKPD 190 200 210 220 230 240 VAFLVAASFF LIVAALGETF LPYYTGRAID SIVIQKSMDQ FTTAVVVVCL LAIGSSLAAG 250 260 270 280 290 300 IRGGIFTLVF ARLNIRLRNC LFRSLVSQET SFFDENRTGD LISRLTSDTT MVSDLVSQNI 310 320 330 340 350 360 NIFLRNTVKV TGVVVFMFSL SWQLSLVTFM GFPIIMMVSN IYGKYYKRLS KEVQSALARA 370 380 390 400 410 420 STTAEETISA MKTVRSFANE EEEAEVFLRK LQQVYKLNRK EAAAYMSYVW GSGLTLLVVQ 430 440 450 460 470 480 VSILYYGGHL VISGQMSSGN LIAFIIYEFV LGDCMESVGS VYSGLMQGVG AAEKVFEFID 490 500 510 520 530 540 RQPTMVHDGS LAPDHLEGRV DFENVTFTYR TRPHTQVLQN VSFSLSPGKV TALVGPSGSG 550 560 570 580 590 600 KSSCVNILEN FYPLQGGRVL LDGKPIGAYD HKYLHRVISL VSQEPVLFAR SITDNISYGL 610 620 630 PTVPFEMVVE AAQKANAHGF IMELQDGYST GTRRRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A half-type ABC transporter TAPL is highly conserved between rodent and man, and the human gene is not responsive to interferon-gamma in contrast to TAP1 and TAP2." Kobayashi A., Kasano M., Maeda T., Hori S., Motojima K., Suzuki M., Fujiwara T., Takahashi E., Yabe T., Tanaka K., Kasahara M., Yamaguchi Y., Maeda M. J. Biochem. 128:711-718(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes." Zhang F., Zhang W., Liu L., Fisher C.L., Hui D., Childs S., Dorovini-Zis K., Ling V. J. Biol. Chem. 275:23287-23294(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Brain.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H. DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 414-762 (ISOFORM 2). Strain: C57BL/6J. Tissue: Brain cortex and Thymus.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB045382 mRNA. Translation: BAA97990.2. AF216495 mRNA. Translation: AAF89994.1. AB093298 mRNA. Translation: BAC41480.1. Different initiation. AK020749 mRNA. No translation available. AK044140 mRNA. Translation: BAC31796.1. BC053014 mRNA. Translation: AAH53014.1.
IPI	IPI00162932. IPI00380188.
RefSeq	NP_063928.2. NM_019875.2.
UniGene	Mm.254839.
3D structure databases
ProteinModelPortal	Q9JJ59.
SMR	Q9JJ59. Positions 171-737.
ModBase	Search...
PTM databases
PhosphoSite	Q9JJ59.
Proteomic databases
PaxDb	Q9JJ59.
PRIDE	Q9JJ59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031354; ENSMUSP00000031354; ENSMUSG00000029408.
GeneID	56325.
KEGG	mmu:56325.
UCSC	uc008zou.1. mouse.
Organism-specific databases
CTD	23457.
MGI	MGI:1861729. Abcb9.
Rouge	Search...
Phylogenomic databases
eggNOG	COG1132.
GeneTree	ENSGT00550000074497.
HOVERGEN	HBG008358.
InParanoid	Q9JJ59.
KO	K05656.
OMA	ANGSHKA.
OrthoDB	EOG4VT5X2.
Gene expression databases
ArrayExpress	Q9JJ59.
Bgee	Q9JJ59.
Genevestigator	Q9JJ59.
GermOnline	ENSMUSG00000029408. Mus musculus.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR013305. ABC_B2. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
PRINTS	PR01896. TAP1PROTEIN.
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	312302.
SOURCE	Search...

Entry information

Entry name

ABCB9_MOUSE

Accession

Primary (citable) accession number: Q9JJ59
Secondary accession number(s): Q8CHA1, Q9D212, Q9JIN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program