Reviewed, UniProtKB/Swiss-Prot Q9JJ46 (EBP_RAT)

Names and origin

Protein names

Recommended name:
    3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    EC=5.3.3.5
Alternative name(s):
    Cholestenol Delta-isomerase
    Delta(8)-Delta(7) sterol isomerase
      Short name=D8-D7 sterol isomerase
    Emopamil-binding protein
    Sterol 8-isomerase

Gene names

Name:	Ebp
Synonyms:	Rsi

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers By similarity.
Catalytic activity	5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Miscellaneous	Binds to the phenylalkylamine calcium-ion antagonist emopamil, an anti-ischemic drug.
Sequence similarities	Belongs to the EBP family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Isomerase
PTM	Acetylation
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: RGD
Molecular function	C-8 sterol isomerase activity Inferred from direct assay. Source: RGD cholestenol delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 230	229	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase		PRO_0000174344
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	121 – 141	21	Potential
	Transmembrane	185 – 205	21	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JJ46-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7CBBF252A0B9E546	FASTA	230	26,738
	10 20 30 40 50 60 MTTNMLPLHP YWPRHLRLDN FVPNDLPTWH ILVGLFSFSG VLIVITWLLS SRVSVVPLGT 70 80 90 100 110 120 GRRLALCWFA VCTFIHLVIE GWFSFYHEIL LEDQAFLSQL WKEYSKGDSR YILSDGFIVC 130 140 150 160 170 180 MESVTACLWG PLSLWVVIAF LRHQPFRFVL QLVVSVGQIY GDVLYFLTEL RDGFQHGELG 190 200 210 220 230 HPLYFWFYFV IMNAIWLVIP GILVFDAIKH LTNAQSMLDN KVMKIKSKHN

References

[1]	"Rat liver sterol 8-isomerase." Bae S.-H., Kim J.-H., Paik Y.-K. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Gene structure and analysis of promoter region of rat sterol 8-isomerase gene." Shin H.-J., Paik Y.-K. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Fischer 344. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF071501 mRNA. Translation: AAF74807.1. AF318617 Genomic DNA. Translation: AAQ14592.1.
IPI	IPI00201003.
RefSeq	NP_476478.1.
UniGene	Rn.19436
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JJ46.
Proteomic databases
PRIDE	Q9JJ46.
Genome annotation databases
Ensembl	ENSRNOT00000007015; ENSRNOP00000007015; ENSRNOG00000004903; Rattus norvegicus. [Genome view]
GeneID	117278.
KEGG	rno:117278.
UCSC	NM_057137. rat.
Organism-specific databases
CTD	117278.
RGD	620957. Ebp.
Phylogenomic databases
eggNOG	roNOG13731.
HOVERGEN	Q9JJ46.
InParanoid	Q9JJ46.
OMA	PTWHILA.
OrthoDB	EOG92JRBS.
PhylomeDB	Q9JJ46.
Enzyme and pathway databases
BRENDA	5.3.3.5. 248.
Gene expression databases
ArrayExpress	Q9JJ46.
Genevestigator	Q9JJ46.
GermOnline	ENSRNOG00000004903. Rattus norvegicus.
Family and domain databases
InterPro	IPR007905. EBP. [Graphical view]
PANTHER	PTHR14207. EBP. 1 hit.
Pfam	PF05241. EBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620221.

Entry information

Entry name

EBP_RAT

Accession

Primary (citable) accession number: Q9JJ46
Secondary accession number(s): Q548M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)