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Protein

3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase

Gene

Ebp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers.By similarity

Catalytic activityi

5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.

Pathwayi: cholesterol biosynthesis

This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

  • C-8 sterol isomerase activity Source: RGD
  • cholestenol delta-isomerase activity Source: UniProtKB-EC

GO - Biological processi

  • cholesterol biosynthetic process Source: RGD
  • hemopoiesis Source: Ensembl
  • sterol biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi5.3.3.5. 5301.
ReactomeiR-RNO-6807047. Cholesterol biosynthesis via desmosterol.
R-RNO-6807062. Cholesterol biosynthesis via lathosterol.
SABIO-RKQ9JJ46.
UniPathwayiUPA00063.

Names & Taxonomyi

Protein namesi
Recommended name:
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EC:5.3.3.5)
Alternative name(s):
Cholestenol Delta-isomerase
Delta(8)-Delta(7) sterol isomerase
Short name:
D8-D7 sterol isomerase
Emopamil-binding protein
Sterol 8-isomerase
Gene namesi
Name:Ebp
Synonyms:Rsi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi620957. Ebp.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4921HelicalSequence analysisAdd
BLAST
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 2302293-beta-hydroxysteroid-Delta(8),Delta(7)-isomerasePRO_0000174344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9JJ46.
PRIDEiQ9JJ46.

Expressioni

Gene expression databases

GenevisibleiQ9JJ46. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4568662.
STRINGi10116.ENSRNOP00000007015.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 204144EXPERAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the EBP family.Curated
Contains 1 EXPERA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4826. Eukaryota.
ENOG41101ES. LUCA.
GeneTreeiENSGT00530000063715.
HOGENOMiHOG000204543.
HOVERGENiHBG018176.
InParanoidiQ9JJ46.
KOiK01824.
OMAiYFTTAYL.
OrthoDBiEOG7P5T1W.
PhylomeDBiQ9JJ46.
TreeFamiTF314716.

Family and domain databases

InterProiIPR007905. EBP.
IPR033118. EXPERA.
[Graphical view]
PANTHERiPTHR14207. PTHR14207. 1 hit.
PfamiPF05241. EBP. 1 hit.
[Graphical view]
PROSITEiPS51751. EXPERA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JJ46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTNMLPLHP YWPRHLRLDN FVPNDLPTWH ILVGLFSFSG VLIVITWLLS
60 70 80 90 100
SRVSVVPLGT GRRLALCWFA VCTFIHLVIE GWFSFYHEIL LEDQAFLSQL
110 120 130 140 150
WKEYSKGDSR YILSDGFIVC MESVTACLWG PLSLWVVIAF LRHQPFRFVL
160 170 180 190 200
QLVVSVGQIY GDVLYFLTEL RDGFQHGELG HPLYFWFYFV IMNAIWLVIP
210 220 230
GILVFDAIKH LTNAQSMLDN KVMKIKSKHN
Length:230
Mass (Da):26,738
Last modified:January 23, 2007 - v3
Checksum:i7CBBF252A0B9E546
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071501 mRNA. Translation: AAF74807.1.
AF318617 Genomic DNA. Translation: AAQ14592.1.
RefSeqiNP_476478.1. NM_057137.1.
XP_006256761.1. XM_006256699.2.
UniGeneiRn.19436.

Genome annotation databases

EnsembliENSRNOT00000007015; ENSRNOP00000007015; ENSRNOG00000004903.
GeneIDi117278.
KEGGirno:117278.
UCSCiRGD:620957. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071501 mRNA. Translation: AAF74807.1.
AF318617 Genomic DNA. Translation: AAQ14592.1.
RefSeqiNP_476478.1. NM_057137.1.
XP_006256761.1. XM_006256699.2.
UniGeneiRn.19436.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4568662.
STRINGi10116.ENSRNOP00000007015.

Proteomic databases

PaxDbiQ9JJ46.
PRIDEiQ9JJ46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007015; ENSRNOP00000007015; ENSRNOG00000004903.
GeneIDi117278.
KEGGirno:117278.
UCSCiRGD:620957. rat.

Organism-specific databases

CTDi10682.
RGDi620957. Ebp.

Phylogenomic databases

eggNOGiKOG4826. Eukaryota.
ENOG41101ES. LUCA.
GeneTreeiENSGT00530000063715.
HOGENOMiHOG000204543.
HOVERGENiHBG018176.
InParanoidiQ9JJ46.
KOiK01824.
OMAiYFTTAYL.
OrthoDBiEOG7P5T1W.
PhylomeDBiQ9JJ46.
TreeFamiTF314716.

Enzyme and pathway databases

UniPathwayiUPA00063.
BRENDAi5.3.3.5. 5301.
ReactomeiR-RNO-6807047. Cholesterol biosynthesis via desmosterol.
R-RNO-6807062. Cholesterol biosynthesis via lathosterol.
SABIO-RKQ9JJ46.

Miscellaneous databases

PROiQ9JJ46.

Gene expression databases

GenevisibleiQ9JJ46. RN.

Family and domain databases

InterProiIPR007905. EBP.
IPR033118. EXPERA.
[Graphical view]
PANTHERiPTHR14207. PTHR14207. 1 hit.
PfamiPF05241. EBP. 1 hit.
[Graphical view]
PROSITEiPS51751. EXPERA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat liver sterol 8-isomerase."
    Bae S.-H., Kim J.-H., Paik Y.-K.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Gene structure and analysis of promoter region of rat sterol 8-isomerase gene."
    Shin H.-J., Paik Y.-K.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Fischer 344.
    Tissue: Liver.

Entry informationi

Entry nameiEBP_RAT
AccessioniPrimary (citable) accession number: Q9JJ46
Secondary accession number(s): Q548M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds to the phenylalkylamine calcium-ion antagonist emopamil, an anti-ischemic drug.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.