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Protein

RNA binding protein fox-1 homolog 1

Gene

Rbfox1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.4 Publications

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • nervous system development Source: GO_Central
  • neuromuscular process controlling balance Source: MGI
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • regulation of skeletal muscle cell differentiation Source: MGI
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA binding protein fox-1 homolog 1
Alternative name(s):
Ataxin-2-binding protein 1
Fox-1 homolog A
Gene namesi
Name:Rbfox1
Synonyms:A2bp, A2bp1, Fox1
ORF Names:MNCb-3035
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1926224. Rbfox1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814811 – 396RNA binding protein fox-1 homolog 1Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei316Asymmetric dimethylarginineCombined sources1
Modified residuei387Omega-N-methylarginineCombined sources1
Isoform 3 (identifier: Q9JJ43-3)
Modified residuei337Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ9JJ43.
PeptideAtlasiQ9JJ43.
PRIDEiQ9JJ43.

PTM databases

iPTMnetiQ9JJ43.
PhosphoSitePlusiQ9JJ43.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Detected in heart, brain, neurons, skeletal muscle and embryo.3 Publications

Gene expression databases

BgeeiENSMUSG00000008658.
CleanExiMM_A2BP1.
GenevisibleiQ9JJ43. MM.

Interactioni

Subunit structurei

Binds to the C-terminus of ATXN2.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei117Interaction with RNABy similarity1
Sitei125Interaction with RNABy similarity1
Sitei126Interaction with RNABy similarity1
Sitei150Interaction with RNABy similarity1
Sitei155Interaction with RNABy similarity1
Sitei159Interaction with RNABy similarity1
Sitei183Interaction with RNABy similarity1
Sitei193Interaction with RNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9JJ43. 2 interactors.
MINTiMINT-4086395.
STRINGi10090.ENSMUSP00000049970.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ43.
SMRiQ9JJ43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 192RRMPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0125. Eukaryota.
ENOG4111WJT. LUCA.
GeneTreeiENSGT00390000013767.
HOVERGENiHBG000044.
InParanoidiQ9JJ43.
KOiK14946.
OMAiSSMYTAP.
OrthoDBiEOG091G15X5.
PhylomeDBiQ9JJ43.
TreeFamiTF315942.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025670. Fox-1_C_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR017325. RNA-bd_Fox-1.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12414. Fox-1_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037932. Ataxin_2_bd_A2BP. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJ43-1) [UniParc]FASTAAdd to basket
Also known as: A016

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP
60 70 80 90 100
EYTGQTTVPD HTLNLYPPTQ THSEQSADTS AQTVSGTATQ TDDAAPTDGQ
110 120 130 140 150
PQTQPSENTE SKSQPKRLHV SNIPFRFRDP DLRQMFGQFG KILDVEIIFN
160 170 180 190 200
ERGSKGFGFV TFENSADADR AREKLHGTVV EGRKIEVNNA TARVMTNKKT
210 220 230 240 250
VNPYTNGWKL NPVVGAVYSP DFYAGTVLLC QANQEGSSMY SGPSSLVYTS
260 270 280 290 300
AMPGFPYPAA TAAAAYRGAH LRGRGRTVYN TFRAAAPPPP IPAYGGVVYQ
310 320 330 340 350
DGFYGADIYG GYAAYRYAQP TPATAAAYSD SYGRVYAADP YHHTLAPAPT
360 370 380 390
YGVGAMNAFA PLTDAKTRSH ADDVGLVLSS LQASIYRGGY NRFAPY
Length:396
Mass (Da):42,678
Last modified:February 5, 2008 - v3
Checksum:iE64DBA6FE6A062ED
GO
Isoform 2 (identifier: Q9JJ43-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:376
Mass (Da):40,492
Checksum:i77C56FE98817B9F2
GO
Isoform 3 (identifier: Q9JJ43-3) [UniParc]FASTAAdd to basket
Also known as: A713

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MNCEREQLR → MLASQGVLLHSYGVPMIVPAAPYFPGLMQ
     301-309: DGFYGADIY → EPVYGNKLLQ

Show »
Length:417
Mass (Da):44,729
Checksum:i95D41CFA0A1D5535
GO
Isoform 4 (identifier: Q9JJ43-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     207-224: Missing.

Show »
Length:378
Mass (Da):40,713
Checksum:i966076D6E685A9C6
GO
Isoform 5 (identifier: Q9JJ43-5) [UniParc]FASTAAdd to basket
Also known as: 2, A030

The sequence of this isoform differs from the canonical sequence as follows:
     331-396: SYGRVYAADP...RGGYNRFAPY → RNQFVFVATD...LQPPPTALVP

Show »
Length:373
Mass (Da):40,288
Checksum:iC20687C2777B463C
GO
Isoform 6 (identifier: Q9JJ43-6) [UniParc]FASTAAdd to basket
Also known as: 4, A715

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MNCEREQLR → MLASQGVLLHSYGVPMIVPAAPYFPGLMQ
     301-309: DGFYGADIY → EPVYGNKLLQ
     331-396: SYGRVYAADP...RGGYNRFAPY → RNQFVFVATD...LQPPPTALVP

Show »
Length:394
Mass (Da):42,338
Checksum:i905CCB2924E570F4
GO
Isoform 7 (identifier: Q9JJ43-7) [UniParc]FASTAAdd to basket
Also known as: 5, A704

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MNCEREQLR → MLASQGVLLHSYGVPMIVPAAPYFPGLMQ
     156-186: Missing.
     301-309: DGFYGADIY → EPVYGNKLLQ
     331-396: SYGRVYAADP...RGGYNRFAPY → RNQFVFVATD...LQPPPTALVP

Show »
Length:363
Mass (Da):38,919
Checksum:iE8A44FBE429F8741
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60D → G in BAA95079 (Ref. 4) Curated1
Sequence conflicti124P → PFR in AAF78292 (PubMed:10814712).Curated1
Sequence conflicti136F → FS in AAF78292 (PubMed:10814712).Curated1
Sequence conflicti261T → S in AAL83425 (Ref. 3) Curated1
Sequence conflicti278V → G in AAL83425 (Ref. 3) Curated1
Sequence conflicti282F → L in AAL83425 (Ref. 3) Curated1
Sequence conflicti369S → G in BAA95079 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0308791 – 20Missing in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_0308801 – 9MNCEREQLR → MLASQGVLLHSYGVPMIVPA APYFPGLMQ in isoform 3, isoform 6 and isoform 7. 3 Publications9
Alternative sequenceiVSP_030881156 – 186Missing in isoform 7. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_030882207 – 224Missing in isoform 4. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_030883301 – 309DGFYGADIY → EPVYGNKLLQ in isoform 3, isoform 6 and isoform 7. 3 Publications9
Alternative sequenceiVSP_030884331 – 396SYGRV…RFAPY → RNQFVFVATDEISCNTSAVT DEFMLPTPTTTHLLQPPPTA LVP in isoform 5, isoform 6 and isoform 7. 3 PublicationsAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107204 mRNA. Translation: AAF78292.1.
AY659954 mRNA. Translation: AAV74337.1.
AY659955 mRNA. Translation: AAV74338.1.
AY659956 mRNA. Translation: AAV74339.1.
AY659957 mRNA. Translation: AAV74340.1.
AY659958 mRNA. Translation: AAV74341.1.
AF191501 mRNA. Translation: AAL83425.1.
AB041596 mRNA. Translation: BAA95079.1.
AK134833 mRNA. Translation: BAE22304.1.
BC059002 mRNA. Translation: AAH59002.1.
CCDSiCCDS27936.1. [Q9JJ43-3]
CCDS37245.1. [Q9JJ43-1]
RefSeqiNP_067452.2. NM_021477.5. [Q9JJ43-1]
NP_899011.2. NM_183188.2. [Q9JJ43-3]
XP_006522251.1. XM_006522188.1. [Q9JJ43-1]
XP_006522252.1. XM_006522189.3. [Q9JJ43-6]
XP_006522253.1. XM_006522190.1. [Q9JJ43-4]
XP_006522255.1. XM_006522192.3. [Q9JJ43-2]
XP_011244213.1. XM_011245911.2. [Q9JJ43-1]
XP_011244218.1. XM_011245916.1. [Q9JJ43-5]
XP_017172487.1. XM_017316998.1. [Q9JJ43-1]
XP_017172491.1. XM_017317002.1. [Q9JJ43-5]
UniGeneiMm.370334.

Genome annotation databases

EnsembliENSMUST00000056416; ENSMUSP00000049970; ENSMUSG00000008658. [Q9JJ43-3]
ENSMUST00000115841; ENSMUSP00000111507; ENSMUSG00000008658. [Q9JJ43-1]
GeneIDi268859.
KEGGimmu:268859.
UCSCiuc007ycb.2. mouse. [Q9JJ43-1]
uc007ycc.2. mouse. [Q9JJ43-4]
uc007ycf.2. mouse. [Q9JJ43-3]
uc007ycg.2. mouse. [Q9JJ43-6]
uc007ych.2. mouse. [Q9JJ43-7]
uc007yci.2. mouse. [Q9JJ43-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107204 mRNA. Translation: AAF78292.1.
AY659954 mRNA. Translation: AAV74337.1.
AY659955 mRNA. Translation: AAV74338.1.
AY659956 mRNA. Translation: AAV74339.1.
AY659957 mRNA. Translation: AAV74340.1.
AY659958 mRNA. Translation: AAV74341.1.
AF191501 mRNA. Translation: AAL83425.1.
AB041596 mRNA. Translation: BAA95079.1.
AK134833 mRNA. Translation: BAE22304.1.
BC059002 mRNA. Translation: AAH59002.1.
CCDSiCCDS27936.1. [Q9JJ43-3]
CCDS37245.1. [Q9JJ43-1]
RefSeqiNP_067452.2. NM_021477.5. [Q9JJ43-1]
NP_899011.2. NM_183188.2. [Q9JJ43-3]
XP_006522251.1. XM_006522188.1. [Q9JJ43-1]
XP_006522252.1. XM_006522189.3. [Q9JJ43-6]
XP_006522253.1. XM_006522190.1. [Q9JJ43-4]
XP_006522255.1. XM_006522192.3. [Q9JJ43-2]
XP_011244213.1. XM_011245911.2. [Q9JJ43-1]
XP_011244218.1. XM_011245916.1. [Q9JJ43-5]
XP_017172487.1. XM_017316998.1. [Q9JJ43-1]
XP_017172491.1. XM_017317002.1. [Q9JJ43-5]
UniGeneiMm.370334.

3D structure databases

ProteinModelPortaliQ9JJ43.
SMRiQ9JJ43.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JJ43. 2 interactors.
MINTiMINT-4086395.
STRINGi10090.ENSMUSP00000049970.

PTM databases

iPTMnetiQ9JJ43.
PhosphoSitePlusiQ9JJ43.

Proteomic databases

PaxDbiQ9JJ43.
PeptideAtlasiQ9JJ43.
PRIDEiQ9JJ43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056416; ENSMUSP00000049970; ENSMUSG00000008658. [Q9JJ43-3]
ENSMUST00000115841; ENSMUSP00000111507; ENSMUSG00000008658. [Q9JJ43-1]
GeneIDi268859.
KEGGimmu:268859.
UCSCiuc007ycb.2. mouse. [Q9JJ43-1]
uc007ycc.2. mouse. [Q9JJ43-4]
uc007ycf.2. mouse. [Q9JJ43-3]
uc007ycg.2. mouse. [Q9JJ43-6]
uc007ych.2. mouse. [Q9JJ43-7]
uc007yci.2. mouse. [Q9JJ43-5]

Organism-specific databases

CTDi54715.
MGIiMGI:1926224. Rbfox1.

Phylogenomic databases

eggNOGiKOG0125. Eukaryota.
ENOG4111WJT. LUCA.
GeneTreeiENSGT00390000013767.
HOVERGENiHBG000044.
InParanoidiQ9JJ43.
KOiK14946.
OMAiSSMYTAP.
OrthoDBiEOG091G15X5.
PhylomeDBiQ9JJ43.
TreeFamiTF315942.

Miscellaneous databases

ChiTaRSiRbfox1. mouse.
PROiQ9JJ43.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008658.
CleanExiMM_A2BP1.
GenevisibleiQ9JJ43. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025670. Fox-1_C_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR017325. RNA-bd_Fox-1.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12414. Fox-1_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037932. Ataxin_2_bd_A2BP. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFOX1_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ43
Secondary accession number(s): Q3UYA8
, Q537C9, Q537D0, Q537D1, Q537D2, Q6PD12, Q8R4Z7, Q9JJB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 5, 2008
Last modified: November 2, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.