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Protein

Protein flightless-1 homolog

Gene

Flii

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling (By similarity). Essential for early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.By similarity4 Publications

Miscellaneous

Flii deficiency causes lethality during early embryogenesis at a stage preceding gastrulation.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin filament severing Source: MGI
  • multicellular organism development Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActin-binding, Activator, Developmental protein
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein flightless-1 homolog
Gene namesi
Name:Flii
Synonyms:Fli1, Fliih
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1342286 Flii

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Increases the percentage of focal complex positive cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187511 – 1271Protein flightless-1 homologAdd BLAST1271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei21N6-acetyllysineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Modified residuei436Phosphoserine; by SGK3By similarity1
Modified residuei860PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JJ28
MaxQBiQ9JJ28
PaxDbiQ9JJ28
PeptideAtlasiQ9JJ28
PRIDEiQ9JJ28

PTM databases

iPTMnetiQ9JJ28
PhosphoSitePlusiQ9JJ28

Expressioni

Tissue specificityi

Expressed in blastocyst.1 Publication

Inductioni

Up-regulated in response to wounding.1 Publication

Gene expression databases

BgeeiENSMUSG00000002812
CleanExiMM_FLI1
MM_FLII
GenevisibleiQ9JJ28 MM

Interactioni

Subunit structurei

Interacts with actin, ACTL6A, NCOA2 and MYD88 (By similarity). Interacts with LRRFIP1 and LRRFIP2. Upon LPS stimulation, LRRFIP2 competes for MYD88-binding. LRRFIP1 constitutively blocks the interaction with MyD88, even in the absence of LPS (By similarity). Interacts with the nuclear receptors ESR1 and THRB (By similarity). Interacts with CARM1. Interacts with SGK3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2dQ6PHZ25EBI-7996161,EBI-2308458

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199703, 6 interactors
DIPiDIP-47641N
IntActiQ9JJ28, 8 interactors
MINTiQ9JJ28
STRINGi10090.ENSMUSP00000002889

Structurei

3D structure databases

ProteinModelPortaliQ9JJ28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati7 – 32LRR 1Add BLAST26
Repeati33 – 55LRR 2Add BLAST23
Repeati56 – 78LRR 3Add BLAST23
Repeati80 – 103LRR 4Add BLAST24
Repeati104 – 126LRR 5Add BLAST23
Repeati127 – 149LRR 6Add BLAST23
Repeati150 – 173LRR 7Add BLAST24
Repeati175 – 196LRR 8Add BLAST22
Repeati197 – 222LRR 9Add BLAST26
Repeati223 – 245LRR 10Add BLAST23
Repeati247 – 268LRR 11Add BLAST22
Repeati269 – 291LRR 12Add BLAST23
Repeati293 – 316LRR 13Add BLAST24
Repeati318 – 339LRR 14Add BLAST22
Repeati340 – 363LRR 15Add BLAST24
Repeati501 – 559Gelsolin-like 1Add BLAST59
Repeati640 – 670Gelsolin-like 2Add BLAST31
Repeati755 – 798Gelsolin-like 3Add BLAST44
Repeati1070 – 1117Gelsolin-like 4Add BLAST48
Repeati1178 – 1220Gelsolin-like 5Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 427Interaction with LRRFIP1 and LRRFIP2By similarityAdd BLAST427
Regioni495 – 827Interaction with ACTL6ABy similarityAdd BLAST333

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi892 – 980Glu-richAdd BLAST89

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0444 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00760000119111
HOGENOMiHOG000016110
HOVERGENiHBG051627
InParanoidiQ9JJ28
OMAiFKNWDDV
OrthoDBiEOG091G00R0
PhylomeDBiQ9JJ28
TreeFamiTF313468

Family and domain databases

Gene3Di3.40.20.10, 5 hits
3.80.10.10, 3 hits
InterProiView protein in InterPro
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR029919 FliI
IPR007123 Gelsolin-like_dom
IPR036180 Gelsolin-like_dom_sf
IPR001611 Leu-rich_rpt
IPR025875 Leu-rich_rpt_4
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR007122 Villin/Gelsolin
PANTHERiPTHR11977 PTHR11977, 1 hit
PTHR11977:SF55 PTHR11977:SF55, 1 hit
PfamiView protein in Pfam
PF00626 Gelsolin, 4 hits
PF12799 LRR_4, 1 hit
PF13855 LRR_8, 3 hits
PRINTSiPR00597 GELSOLIN
SMARTiView protein in SMART
SM00262 GEL, 6 hits
SM00369 LRR_TYP, 9 hits
SUPFAMiSSF82754 SSF82754, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 11 hits

Sequencei

Sequence statusi: Complete.

Q9JJ28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATGVLPFV RGVDLSGNDF KGGYFPENVK AMTSLRWLKL NRTGLCYLPE
60 70 80 90 100
ELAALQKLEH LSVSHNHLTT LHGELSSLPS LRAIVARANS LKNSGVPDDI
110 120 130 140 150
FKLDDLSVLD LSHNQLTECP RELENAKNML VLNLSHNGID SIPNQLFINL
160 170 180 190 200
TDLLYLDLSE NRLESLPPQM RRLVHLQTLV LNGNPLLHAQ LRQLPAMMAL
210 220 230 240 250
QTLHLRNTQR TQSNLPTSLE GLSNLSDVDL SCNDLTRVPE CLYTLPSLRR
260 270 280 290 300
LNLSSNQIAE LSLCIDQWVH LETLNLSRNQ LTSLPSAICK LTKLKKLYLN
310 320 330 340 350
SNKLDFDGLP SGIGKLTSLE EFMAANNNLE LIPESLCRCP KLKKLVLNKN
360 370 380 390 400
RLVTLPEAIH FLTEIQVLDV RENPSLVMPP KPADRTAEWY NIDFSLQNQL
410 420 430 440 450
RLAGASPATV AAAAAVGSGS KDPLARKMRL RRRKDSAQDV QAKQVLKGMS
460 470 480 490 500
DVAQEKNKNQ EESIDARAPG GKVRRWDQGL EKPRLDYSEF FTEDVGQLPG
510 520 530 540 550
LTIWQIENFV PVLVEEAFHG KFYEADCYIV LKTFLDDSGS LNWEIYYWIG
560 570 580 590 600
GEATLDKKAC SAIHAVNLRN YLGAECRTVR EEMGDESEEF LQVFDNDISY
610 620 630 640 650
IEGGTASGFY TVEDTHYVTR MYRVYGKKNI KLEPVPLKGS SLDPRFVFLL
660 670 680 690 700
DQGLDIYVWR GAQATLSNTT KARLFAEKIN KNERKGKAEI TLLVQGQEPP
710 720 730 740 750
GFWDVLGGEP SEIKNHVPDD FWPPQPKLYK VGLGLGYLEL PQINYKLSVE
760 770 780 790 800
HKKRPKVELM PGMRLLQSLL DTRCVYILDC WSDVFIWLGR KSPRLVRAAA
810 820 830 840 850
LKLGQELCGM LHRPRHTVVS RSLEGTEAQV FKAKFKNWDD VLTVDYTRNA
860 870 880 890 900
EAVLQGQGLS GKVKRDTEKT DQMKADLTAL FLPRQPPMPL AEAEQLMEEW
910 920 930 940 950
NEDLDGMEGF VLEGRKFTRL PEEEFGHFYT QDCYVFLCRY WVPVEYEEEE
960 970 980 990 1000
KTEDKEGKAS AEAREGEEAA AEAEEKQPEE DFQCIVYFWQ GREASNMGWL
1010 1020 1030 1040 1050
TFTFSLQKKF ESLFPGKLEV VRMTQQQENP KFLSHFKRKF IIHRGKRKVT
1060 1070 1080 1090 1100
QGTLQPTLYQ IRTNGSALCT RCIQINTDSS LLNSEFCFIL KVPFESEDNQ
1110 1120 1130 1140 1150
GIVYAWVGRA SDPDEAKLAE DILNTMFDAS YSKQVINEGE EPENFFWVGI
1160 1170 1180 1190 1200
GAQKPYDDDA EYMKHTRLFR CSNEKGYFAV TEKCSDFCQD DLADDDIMLL
1210 1220 1230 1240 1250
DNGQEVYMWV GTQTSQVEIK LSLKACQVYI QHTRSKEHER PRRLRLVRKG
1260 1270
NEQRAFTRCF HAWSTFRQAP A
Length:1,271
Mass (Da):144,803
Last modified:October 1, 2000 - v1
Checksum:iA9642B10FEBF8769
GO

Sequence cautioni

The sequence AAH32282 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti849N → D in AAL36557 (PubMed:10902907).Curated1
Sequence conflicti1057T → I in AAH32282 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142329 Genomic DNA Translation: AAF78453.1
AF287264 mRNA Translation: AAL36557.1
AL596215 Genomic DNA Translation: CAI35270.1
BC027744 mRNA Translation: AAH27744.1
BC032282 mRNA Translation: AAH32282.1 Different initiation.
CCDSiCCDS24795.1
RefSeqiNP_071292.1, NM_022009.2
UniGeneiMm.339755

Genome annotation databases

EnsembliENSMUST00000002889; ENSMUSP00000002889; ENSMUSG00000002812
GeneIDi14248
KEGGimmu:14248
UCSCiuc007jgh.2 mouse

Similar proteinsi

Entry informationi

Entry nameiFLII_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ28
Secondary accession number(s): Q8K095, Q8VI44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health