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Protein

Protein flightless-1 homolog

Gene

Flii

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling (By similarity). Essential for early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin filament severing Source: MGI
  • multicellular organism development Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein flightless-1 homolog
Gene namesi
Name:Flii
Synonyms:Fli1, Fliih
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1342286. Flii.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • cytoplasm Source: MGI
  • focal adhesion Source: UniProtKB-SubCell
  • microtubule organizing center Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Increases the percentage of focal complex positive cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187511 – 1271Protein flightless-1 homologAdd BLAST1271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei21N6-acetyllysineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Modified residuei436Phosphoserine; by SGK3By similarity1
Modified residuei860PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JJ28.
MaxQBiQ9JJ28.
PaxDbiQ9JJ28.
PeptideAtlasiQ9JJ28.
PRIDEiQ9JJ28.

PTM databases

iPTMnetiQ9JJ28.
PhosphoSitePlusiQ9JJ28.

Expressioni

Tissue specificityi

Expressed in blastocyst.1 Publication

Inductioni

Up-regulated in response to wounding.1 Publication

Gene expression databases

BgeeiENSMUSG00000002812.
CleanExiMM_FLI1.
MM_FLII.
GenevisibleiQ9JJ28. MM.

Interactioni

Subunit structurei

Interacts with actin, ACTL6A, NCOA2 and MYD88 (By similarity). Interacts with LRRFIP1 and LRRFIP2. Upon LPS stimulation, LRRFIP2 competes for MYD88-binding. LRRFIP1 constitutively blocks the interaction with MyD88, even in the absence of LPS (By similarity). Interacts with the nuclear receptors ESR1 and THRB (By similarity). Interacts with CARM1. Interacts with SGK3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2dQ6PHZ25EBI-7996161,EBI-2308458

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199703. 4 interactors.
DIPiDIP-47641N.
IntActiQ9JJ28. 6 interactors.
MINTiMINT-4095146.
STRINGi10090.ENSMUSP00000002889.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ28.
SMRiQ9JJ28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati7 – 32LRR 1Add BLAST26
Repeati33 – 55LRR 2Add BLAST23
Repeati56 – 78LRR 3Add BLAST23
Repeati80 – 103LRR 4Add BLAST24
Repeati104 – 126LRR 5Add BLAST23
Repeati127 – 149LRR 6Add BLAST23
Repeati150 – 173LRR 7Add BLAST24
Repeati175 – 196LRR 8Add BLAST22
Repeati197 – 222LRR 9Add BLAST26
Repeati223 – 245LRR 10Add BLAST23
Repeati247 – 268LRR 11Add BLAST22
Repeati269 – 291LRR 12Add BLAST23
Repeati293 – 316LRR 13Add BLAST24
Repeati318 – 339LRR 14Add BLAST22
Repeati340 – 363LRR 15Add BLAST24
Repeati501 – 559Gelsolin-like 1Add BLAST59
Repeati640 – 670Gelsolin-like 2Add BLAST31
Repeati755 – 798Gelsolin-like 3Add BLAST44
Repeati1070 – 1117Gelsolin-like 4Add BLAST48
Repeati1178 – 1220Gelsolin-like 5Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 427Interaction with LRRFIP1 and LRRFIP2By similarityAdd BLAST427
Regioni495 – 827Interaction with ACTL6ABy similarityAdd BLAST333

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi892 – 980Glu-richAdd BLAST89

Sequence similaritiesi

Contains 5 gelsolin-like repeats.Curated
Contains 15 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0444. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000016110.
HOVERGENiHBG051627.
InParanoidiQ9JJ28.
OMAiQMKADLT.
OrthoDBiEOG091G00R0.
PhylomeDBiQ9JJ28.
TreeFamiTF313468.

Family and domain databases

Gene3Di3.40.20.10. 7 hits.
3.80.10.10. 2 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR029919. FliI.
IPR007123. Gelsolin-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF42. PTHR11977:SF42. 2 hits.
PfamiPF00626. Gelsolin. 4 hits.
PF12799. LRR_4. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00369. LRR_TYP. 9 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF82754. SSF82754. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJ28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATGVLPFV RGVDLSGNDF KGGYFPENVK AMTSLRWLKL NRTGLCYLPE
60 70 80 90 100
ELAALQKLEH LSVSHNHLTT LHGELSSLPS LRAIVARANS LKNSGVPDDI
110 120 130 140 150
FKLDDLSVLD LSHNQLTECP RELENAKNML VLNLSHNGID SIPNQLFINL
160 170 180 190 200
TDLLYLDLSE NRLESLPPQM RRLVHLQTLV LNGNPLLHAQ LRQLPAMMAL
210 220 230 240 250
QTLHLRNTQR TQSNLPTSLE GLSNLSDVDL SCNDLTRVPE CLYTLPSLRR
260 270 280 290 300
LNLSSNQIAE LSLCIDQWVH LETLNLSRNQ LTSLPSAICK LTKLKKLYLN
310 320 330 340 350
SNKLDFDGLP SGIGKLTSLE EFMAANNNLE LIPESLCRCP KLKKLVLNKN
360 370 380 390 400
RLVTLPEAIH FLTEIQVLDV RENPSLVMPP KPADRTAEWY NIDFSLQNQL
410 420 430 440 450
RLAGASPATV AAAAAVGSGS KDPLARKMRL RRRKDSAQDV QAKQVLKGMS
460 470 480 490 500
DVAQEKNKNQ EESIDARAPG GKVRRWDQGL EKPRLDYSEF FTEDVGQLPG
510 520 530 540 550
LTIWQIENFV PVLVEEAFHG KFYEADCYIV LKTFLDDSGS LNWEIYYWIG
560 570 580 590 600
GEATLDKKAC SAIHAVNLRN YLGAECRTVR EEMGDESEEF LQVFDNDISY
610 620 630 640 650
IEGGTASGFY TVEDTHYVTR MYRVYGKKNI KLEPVPLKGS SLDPRFVFLL
660 670 680 690 700
DQGLDIYVWR GAQATLSNTT KARLFAEKIN KNERKGKAEI TLLVQGQEPP
710 720 730 740 750
GFWDVLGGEP SEIKNHVPDD FWPPQPKLYK VGLGLGYLEL PQINYKLSVE
760 770 780 790 800
HKKRPKVELM PGMRLLQSLL DTRCVYILDC WSDVFIWLGR KSPRLVRAAA
810 820 830 840 850
LKLGQELCGM LHRPRHTVVS RSLEGTEAQV FKAKFKNWDD VLTVDYTRNA
860 870 880 890 900
EAVLQGQGLS GKVKRDTEKT DQMKADLTAL FLPRQPPMPL AEAEQLMEEW
910 920 930 940 950
NEDLDGMEGF VLEGRKFTRL PEEEFGHFYT QDCYVFLCRY WVPVEYEEEE
960 970 980 990 1000
KTEDKEGKAS AEAREGEEAA AEAEEKQPEE DFQCIVYFWQ GREASNMGWL
1010 1020 1030 1040 1050
TFTFSLQKKF ESLFPGKLEV VRMTQQQENP KFLSHFKRKF IIHRGKRKVT
1060 1070 1080 1090 1100
QGTLQPTLYQ IRTNGSALCT RCIQINTDSS LLNSEFCFIL KVPFESEDNQ
1110 1120 1130 1140 1150
GIVYAWVGRA SDPDEAKLAE DILNTMFDAS YSKQVINEGE EPENFFWVGI
1160 1170 1180 1190 1200
GAQKPYDDDA EYMKHTRLFR CSNEKGYFAV TEKCSDFCQD DLADDDIMLL
1210 1220 1230 1240 1250
DNGQEVYMWV GTQTSQVEIK LSLKACQVYI QHTRSKEHER PRRLRLVRKG
1260 1270
NEQRAFTRCF HAWSTFRQAP A
Length:1,271
Mass (Da):144,803
Last modified:October 1, 2000 - v1
Checksum:iA9642B10FEBF8769
GO

Sequence cautioni

The sequence AAH32282 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti849N → D in AAL36557 (PubMed:10902907).Curated1
Sequence conflicti1057T → I in AAH32282 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142329 Genomic DNA. Translation: AAF78453.1.
AF287264 mRNA. Translation: AAL36557.1.
AL596215 Genomic DNA. Translation: CAI35270.1.
BC027744 mRNA. Translation: AAH27744.1.
BC032282 mRNA. Translation: AAH32282.1. Different initiation.
CCDSiCCDS24795.1.
RefSeqiNP_071292.1. NM_022009.2.
UniGeneiMm.339755.

Genome annotation databases

EnsembliENSMUST00000002889; ENSMUSP00000002889; ENSMUSG00000002812.
GeneIDi14248.
KEGGimmu:14248.
UCSCiuc007jgh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142329 Genomic DNA. Translation: AAF78453.1.
AF287264 mRNA. Translation: AAL36557.1.
AL596215 Genomic DNA. Translation: CAI35270.1.
BC027744 mRNA. Translation: AAH27744.1.
BC032282 mRNA. Translation: AAH32282.1. Different initiation.
CCDSiCCDS24795.1.
RefSeqiNP_071292.1. NM_022009.2.
UniGeneiMm.339755.

3D structure databases

ProteinModelPortaliQ9JJ28.
SMRiQ9JJ28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199703. 4 interactors.
DIPiDIP-47641N.
IntActiQ9JJ28. 6 interactors.
MINTiMINT-4095146.
STRINGi10090.ENSMUSP00000002889.

PTM databases

iPTMnetiQ9JJ28.
PhosphoSitePlusiQ9JJ28.

Proteomic databases

EPDiQ9JJ28.
MaxQBiQ9JJ28.
PaxDbiQ9JJ28.
PeptideAtlasiQ9JJ28.
PRIDEiQ9JJ28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002889; ENSMUSP00000002889; ENSMUSG00000002812.
GeneIDi14248.
KEGGimmu:14248.
UCSCiuc007jgh.2. mouse.

Organism-specific databases

CTDi2314.
MGIiMGI:1342286. Flii.

Phylogenomic databases

eggNOGiKOG0444. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000016110.
HOVERGENiHBG051627.
InParanoidiQ9JJ28.
OMAiQMKADLT.
OrthoDBiEOG091G00R0.
PhylomeDBiQ9JJ28.
TreeFamiTF313468.

Miscellaneous databases

ChiTaRSiFlii. mouse.
PROiQ9JJ28.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002812.
CleanExiMM_FLI1.
MM_FLII.
GenevisibleiQ9JJ28. MM.

Family and domain databases

Gene3Di3.40.20.10. 7 hits.
3.80.10.10. 2 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR029919. FliI.
IPR007123. Gelsolin-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF42. PTHR11977:SF42. 2 hits.
PfamiPF00626. Gelsolin. 4 hits.
PF12799. LRR_4. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00369. LRR_TYP. 9 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF82754. SSF82754. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLII_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ28
Secondary accession number(s): Q8K095, Q8VI44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Flii deficiency causes lethality during early embryogenesis at a stage preceding gastrulation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.