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Protein

Pyrin

Gene

Mefv

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri440 – 48243B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrin
Alternative name(s):
Marenostrin
Gene namesi
Name:Mefv
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61889. Mefv.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell projectionruffle By similarity
  • Cell projectionlamellipodium By similarity
  • Cytoplasm By similarity
  • Cytoplasmic vesicleautophagosome By similarity
  • Nucleus By similarity

  • Note: Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. In pre-apoptotic cells, colocalizes with PYCARD/ASC in large specks (pyroptosomes). In migrating monocytes, strongly polarized at the leading edge of the cell where it colocalizes with polymerizing actin and PYCARD/ASC (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750PyrinPRO_0000220366Add
BLAST

Proteomic databases

PaxDbiQ9JJ25.
PRIDEiQ9JJ25.

PTM databases

iPTMnetiQ9JJ25.

Expressioni

Tissue specificityi

Expressed in spleen and, to a lesser degree in the lung. Not expressed in thymus, testis, ovary, heart, brain, liver, kidney and muscle.1 Publication

Interactioni

Subunit structurei

Homotrimer. Interacts (via the B box-type zinc finger) with PSTPIP1. Interacts (via the B30.2/SPRY domain) with several components of the inflammasome complex, including CASP1 p20 and p10 subunits, CASP5, PYCARD, NLRP1, NLRP2 AND NLRP3, as well as with unprocessed IL1B; this interaction may lead to autophagic degradation of these proteins. Interacts with NFKBIA and RELA. Interacts weakly with VASP and ACTR3. Interacts with active ULK1 (phosphorylated on 'Ser-317') and BECN1 simultaneously. Also interacts with ATG16L1 (via WD repeats), and with ATG8 family members, including GABARAP, GABARAPL1 and, to a lesser extent, GABARAPL2, MAP1LC3A/LC3A and MAP1LC3C/LC3C. Interacts with TRIM21.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011073.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9292PyrinPROSITE-ProRule annotationCuratedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni490 – 648159Required for homotrimerization and induction of pyroptosomesBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili482 – 51130Sequence analysisAdd
BLAST

Domaini

The B box-type zinc finger interacts, possibly intramolecularly, with the pyrin domain; this may be an autoinhibitory mechanism released by PSTPIP1 binding.By similarity

Sequence similaritiesi

Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 pyrin domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri440 – 48243B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
HOGENOMiHOG000113552.
HOVERGENiHBG006343.
InParanoidiQ9JJ25.
KOiK12803.
PhylomeDBiQ9JJ25.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR004020. DAPIN.
IPR011029. DEATH-like_dom.
IPR000315. Znf_B-box.
[Graphical view]
PfamiPF02758. PYRIN. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 1 hit.
SM01289. PYRIN. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50824. DAPIN. 1 hit.
PS50119. ZF_BBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JJ25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTRVDHLL NTLEELLPYE LEKFKFKLHT TSLEKGHSRI PLSLVKMARP
60 70 80 90 100
IKLTRLLLTY YGEEYAVRLT LQILRATNQR QLAEELHKAT GPEHLTEENG
110 120 130 140 150
VGGSVQSSAE NKDKGVKGSD VLGEDEAQQN DDESDILPPI QAEVGKGPQK
160 170 180 190 200
KSLAKRKDQR GPESLDSQTK PGARSAAPLY RRTLVTQSPG DKENRAGAQT
210 220 230 240 250
PQNAQLCREA ARNSTAMSQG GERSRRLKCI CLQERSDPGV LKLPLTQKKE
260 270 280 290 300
NPQIQKLFRL KRKQEMAVSF VRETATLNGR TTGTLEKGVG IPEHSMMLDE
310 320 330 340 350
ETSRNMSSKI SLTREKRCTA SWTENGNGGP ETPETLGETV SSILCDSCSP
360 370 380 390 400
KVLLSLGEKL AQTPEDPASL GQAASKGRSR DKVACPLCHT QGELPAKACV
410 420 430 440 450
QSSCSCSVAP GDPKASGRCS ICFQCQSSRA GKSCEAQSPQ FLPQCPRHMK
460 470 480 490 500
QVQLLFCEDH REPICLICRL SQEHQGHRVR PIEEAALQYK EQIRKQLERL
510 520 530 540 550
REMRGYVEEH KLPADKKAED FLKQTETQKQ RISCPLEKLF QFLEQQEQLF
560 570 580 590 600
VTWLQELVQT IGKVRETYYT QVSLLDKLIG ELEAKQDQPE WELMQDIGAT
610 620 630 640 650
LHRAETMTAS ELLGIPPGVK EKLHLLYQKS KSAEKNMQRF SEMLGSEMAF
660 670 680 690 700
SASDVATREG CRPSTTKAQA LIPTVHLKCD GAHTQDFDVI LCAELEAGGS
710 720 730 740 750
EPQDYLHPSS AQDTPELHEI HSQNNKRKFK SFLKWKPSFS RTDRCLRTCW
Length:750
Mass (Da):83,995
Last modified:October 1, 2000 - v1
Checksum:iC76D0F3E02711312
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143410 mRNA. Translation: AAF03767.1.
RefSeqiNP_113822.1. NM_031634.1.
UniGeneiRn.29105.

Genome annotation databases

GeneIDi58923.
KEGGirno:58923.
UCSCiRGD:61889. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143410 mRNA. Translation: AAF03767.1.
RefSeqiNP_113822.1. NM_031634.1.
UniGeneiRn.29105.

3D structure databases

ProteinModelPortaliQ9JJ25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011073.

PTM databases

iPTMnetiQ9JJ25.

Proteomic databases

PaxDbiQ9JJ25.
PRIDEiQ9JJ25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58923.
KEGGirno:58923.
UCSCiRGD:61889. rat.

Organism-specific databases

CTDi4210.
RGDi61889. Mefv.

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
HOGENOMiHOG000113552.
HOVERGENiHBG006343.
InParanoidiQ9JJ25.
KOiK12803.
PhylomeDBiQ9JJ25.

Miscellaneous databases

NextBioi611486.
PROiQ9JJ25.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR004020. DAPIN.
IPR011029. DEATH-like_dom.
IPR000315. Znf_B-box.
[Graphical view]
PfamiPF02758. PYRIN. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 1 hit.
SM01289. PYRIN. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50824. DAPIN. 1 hit.
PS50119. ZF_BBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation, genomic organization, and expression analysis of the mouse and rat homologs of MEFV, the gene for familial Mediterranean fever."
    Chae J.J., Centola M., Aksentijevich I., Dutra A., Tran M., Wood G., Nagaraju K., Kingma D.W., Liu P.P., Kastner D.L.
    Mamm. Genome 11:428-435(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiMEFV_RAT
AccessioniPrimary (citable) accession number: Q9JJ25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Degraded along with the delivery of its substrates to autolysosomal compartments (at protein level).By similarity

Caution

Lacks the B30.2/SPRY domain found in the human ortholog, thus may have divergent function(s).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.