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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

Gene

Slc9a3r1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules (By similarity).By similarity

GO - Molecular functioni

  • chloride channel regulator activity Source: UniProtKB
  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD
  • protein complex scaffold Source: RGD
  • protein domain specific binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Short name:
NHERF-1
Alternative name(s):
Ezrin-radixin-moesin-binding phosphoprotein 50
Short name:
EBP50
Regulatory cofactor of Na(+)/H(+) exchanger
Sodium-hydrogen exchanger regulatory factor 1
Solute carrier family 9 isoform A3 regulatory factor 1
Gene namesi
Name:Slc9a3r1
Synonyms:Nherf, Nherf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi708538. Slc9a3r1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • brush border membrane Source: Ensembl
  • centrosome Source: Ensembl
  • cytoplasm Source: UniProtKB
  • endomembrane system Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • filopodium Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: Ensembl
  • membrane Source: UniProtKB
  • membrane raft Source: RGD
  • microvillus membrane Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
  • ruffle Source: UniProtKB-SubCell
  • sperm midpiece Source: UniProtKB
  • stereocilium tip Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 356355Na(+)/H(+) exchange regulatory cofactor NHE-RF1PRO_0000096802Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei266 – 2661PhosphoserineBy similarity
Modified residuei277 – 2771PhosphoserineCombined sources
Modified residuei287 – 2871PhosphoserineCombined sources
Modified residuei288 – 2881PhosphoserineCombined sources
Modified residuei290 – 2901PhosphothreonineCombined sources
Modified residuei291 – 2911PhosphoserineCombined sources
Modified residuei299 – 2991PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JJ19.
PRIDEiQ9JJ19.

PTM databases

iPTMnetiQ9JJ19.
PhosphoSiteiQ9JJ19.

Expressioni

Gene expression databases

GenevisibleiQ9JJ19. RN.

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2, CFTR and PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1, PLCB3, PDZK1 and CLCN3. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1 (By similarity). Interacts with SLC4A7. Directly interacts with HTR4 (By similarity). Interacts with TRPC4 (via the PDZ-binding domain) (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells. Interacts with SLC34A1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity). Interacts with SLC26A3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Asic3O352405EBI-982391,EBI-982374

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • protein complex binding Source: RGD
  • protein complex scaffold Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi248735. 2 interactions.
IntActiQ9JJ19. 1 interaction.
MINTiMINT-4568572.
STRINGi10116.ENSRNOP00000004351.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ19.
SMRiQ9JJ19. Positions 11-99, 147-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 9481PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini151 – 23181PDZ 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITAB. Eukaryota.
ENOG4110SZZ. LUCA.
GeneTreeiENSGT00530000062999.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ9JJ19.
KOiK13365.
OMAiAMKKGPN.
OrthoDBiEOG7R56T6.
PhylomeDBiQ9JJ19.
TreeFamiTF350449.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR031199. NHERF-1.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF7. PTHR14191:SF7. 1 hit.
PfamiPF09007. EBP50_C. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JJ19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADAAAGEP LPRLCCLEKG PNGYGFHLHG EKGKVGQFIR LVEPGSPAEK
60 70 80 90 100
SGLLAGDRLV EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLK
110 120 130 140 150
KLGVPIREEL LRAQEKSEHT EPPAAADTKK AGDQNEAEKS HLERCELRPR
160 170 180 190 200
LCTMKKGPNG YGFNLHSDKS KPGQFIRAVD PDSPAEASGL RAQDRIVEVN
210 220 230 240 250
GVCMEGKQHG DVVSAIKAGG DEAKLLVVDK ETDEFFKKCR VTPSQEHLDG
260 270 280 290 300
PLPEPFSNGE IQKENSREAL VEPASESPRP ALARSASSDT SEELNAQDSP
310 320 330 340 350
KRHDSTEPSS TSSSSDPILD FNISLAVAKE RAHQKRSSKR APQMDWSKKN

ELFSNL
Length:356
Mass (Da):38,830
Last modified:January 23, 2007 - v3
Checksum:iC562AEF5870B8E08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF154336 mRNA. Translation: AAF73258.1.
RefSeqiNP_067605.1. NM_021594.1.
UniGeneiRn.35142.

Genome annotation databases

EnsembliENSRNOT00000004351; ENSRNOP00000004351; ENSRNOG00000003232.
GeneIDi59114.
KEGGirno:59114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF154336 mRNA. Translation: AAF73258.1.
RefSeqiNP_067605.1. NM_021594.1.
UniGeneiRn.35142.

3D structure databases

ProteinModelPortaliQ9JJ19.
SMRiQ9JJ19. Positions 11-99, 147-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248735. 2 interactions.
IntActiQ9JJ19. 1 interaction.
MINTiMINT-4568572.
STRINGi10116.ENSRNOP00000004351.

PTM databases

iPTMnetiQ9JJ19.
PhosphoSiteiQ9JJ19.

Proteomic databases

PaxDbiQ9JJ19.
PRIDEiQ9JJ19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004351; ENSRNOP00000004351; ENSRNOG00000003232.
GeneIDi59114.
KEGGirno:59114.

Organism-specific databases

CTDi9368.
RGDi708538. Slc9a3r1.

Phylogenomic databases

eggNOGiENOG410ITAB. Eukaryota.
ENOG4110SZZ. LUCA.
GeneTreeiENSGT00530000062999.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ9JJ19.
KOiK13365.
OMAiAMKKGPN.
OrthoDBiEOG7R56T6.
PhylomeDBiQ9JJ19.
TreeFamiTF350449.

Miscellaneous databases

NextBioi611763.
PROiQ9JJ19.

Gene expression databases

GenevisibleiQ9JJ19. RN.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR031199. NHERF-1.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF7. PTHR14191:SF7. 1 hit.
PfamiPF09007. EBP50_C. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence for ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) self-association through PDZ-PDZ interactions."
    Fouassier L., Yun C.H.C., Fitz J.G., Doctor R.B.
    J. Biol. Chem. 275:25039-25045(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DIMERIZATION.
    Tissue: Liver.
  2. "Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton."
    Takeda T., McQuistan T., Orlando R.A., Farquhar M.G.
    J. Clin. Invest. 108:289-301(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PODXL.
  3. "The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction."
    Pushkin A., Abuladze N., Newman D., Muronets V., Sassani P., Tatishchev S., Kurtz I.
    Am. J. Physiol. 284:C667-C673(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC4A7.
  4. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
    Moser K., White F.M.
    J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-277; SER-287; SER-288; THR-290; SER-291 AND SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNHRF1_RAT
AccessioniPrimary (citable) accession number: Q9JJ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.