Q9JJ16 (5HT3B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 3B
Short name=5-HT3-B
Short name=5-HT3B

Alternative name(s):
Serotonin receptor 3B

Gene names

Name:

Htr3b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. Ref.3
Subunit structure	Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence and antagonist affinity. Ref.1 Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A By similarity.
Tissue specificity	Expressed in peripheral neurons, but not in neurons of the central nervous system. Ref.2
Induction	By nerve growth factor in PC12 cells. Ref.1
Post-translational modification	N-glycosylation is required for membrane localization By similarity.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to growth factor stimulus Inferred from expression pattern Ref.1. Source: RGD
Cellular component	axon Inferred from direct assay. Source: RGD neuronal cell body Inferred from direct assay. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: InterPro
Molecular function	serotonin receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 437	416	5-hydroxytryptamine receptor 3B		PRO_0000312291
Regions
	Topological domain	22 – 235	214	Extracellular Potential
	Transmembrane	236 – 255	20	Helical; Name=1; Potential
	Topological domain	256 – 266	11	Cytoplasmic Potential
	Transmembrane	267 – 284	18	Helical; Name=2; Potential
	Topological domain	285 – 295	11	Extracellular Potential
	Transmembrane	296 – 324	29	Helical; Name=3; Potential
	Topological domain	325 – 410	86	Cytoplasmic Potential
	Transmembrane	411 – 434	24	Helical; Name=4; Potential
	Topological domain	435 – 437	3	Extracellular Potential
	Region	377 – 409	33	HA-stretch
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Disulfide bond	151 ↔ 165		By similarity
Experimental info
	Sequence conflict	194	1	K → E in AAG30903. Ref.1
	Sequence conflict	217	1	S → N in AAG30903. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9JJ16 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 633C2F4175FDB55A	FASTA	437	50,336
	10 20 30 40 50 60 MILLWSCLLV AVVGILGTAT PQPGNSSLHR LTRQLLQQYH KEVRPVYNWA EATTVYLDLC 70 80 90 100 110 120 VHAVLDVDVQ NQKLKTSMWY REVWNDEFLS WNSSLFDDIQ EISLPLSAIW APDIIINEFV 130 140 150 160 170 180 DVERSPDLPY VYVNSSGTIR NHKPIQVVSA CSLQTYAFPF DIQNCSLTFN SILHTVEDID 190 200 210 220 230 240 LGFLRNQEDI ENDKRSFLND SEWQLLSVTS TYHIRQSSAG DFAQIRFNVV IRRCPLAYVV 250 260 270 280 290 300 SLLIPSIFLM LVDLGSFYLP PNCRARIVFK TNVLVGYTVF RVNMSDEVPR SAGCTSLIGV 310 320 330 340 350 360 FFTVCMALLV LSLSKSILLI KFLYEERHSE QERPLMCLRG DSDANESRLY LRAPCAEDTE 370 380 390 400 410 420 SPVRQEHQVP SDTLKDFWFQ LQSINNSLRT RDQVYQKEVE WLAILCHFDQ LLFRIYLAVL 430 GLYTVTLCSL WALWSRM

References

[1]	"Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents." Hanna M.C., Davies P.A., Hales T.G., Kirkness E.F. J. Neurochem. 75:240-247(2000) [PubMed: 10854267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INDUCTION. Strain: Sprague-Dawley.
[2]	"Differential composition of 5-hydroxytryptamine3 receptors synthesized in the rat CNS and peripheral nervous system." Morales M., Wang S.-D. J. Neurosci. 22:6732-6741(2002) [PubMed: 12151552] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY.
[3]	"A cytoplasmic region determines single-channel conductance in 5-HT3 receptors." Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A. Nature 424:321-324(2003) [PubMed: 12867984] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155044 mRNA. Translation: AAF73283.1. AF303447 mRNA. Translation: AAG30903.1.
IPI	IPI00200894.
RefSeq	NP_071525.1. NM_022189.1.
UniGene	Rn.87106.
3D structure databases
HSSP	HSSP built from PDB template 2BG9 based on UniProtKB P02711.
ProteinModelPortal	Q9JJ16.
ModBase	Search...
Protein family/group databases
TCDB	1.A.9.2.2. neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.
Proteomic databases
PRIDE	Q9JJ16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	58963.
KEGG	rno:58963.
Organism-specific databases
CTD	9177.
RGD	61820. Htr3b.
Phylogenomic databases
eggNOG	roNOG05248.
GeneTree	ENSGT00570000078866.
HOVERGEN	HBG106638.
InParanoid	Q9JJ16.
OrthoDB	EOG4N8R4Z.
Gene expression databases
ArrayExpress	Q9JJ16.
Genevestigator	Q9JJ16.
Family and domain databases
InterPro	IPR008132. 5HT3_rcpt. IPR008134. 5HT3_rcpt_B. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K04819.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR01710. 5HT3BRECEPTR. PR01708. 5HT3RECEPTOR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	611608.

Entry information

Entry name

5HT3B_RAT

Accession

Primary (citable) accession number: Q9JJ16
Secondary accession number(s): Q9ERJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program