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Protein

Transforming acidic coiled-coil-containing protein 3

Gene

Tacc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (PubMed:17920017). Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (By similarity). May be involved in the control of cell growth and differentiation. May have a role in embryonic development.By similarity1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: MGI

GO - Biological processi

  • astral microtubule organization Source: MGI
  • cell division Source: UniProtKB-KW
  • cell proliferation Source: MGI
  • cerebral cortex development Source: MGI
  • cytoplasmic sequestering of transcription factor Source: MGI
  • hemopoiesis Source: MGI
  • interkinetic nuclear migration Source: MGI
  • metaphase/anaphase transition of mitotic cell cycle Source: MGI
  • microtubule cytoskeleton organization Source: MGI
  • microtubule cytoskeleton organization involved in mitosis Source: MGI
  • neurogenesis Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of microtubule-based process Source: MGI
  • regulation of mitotic spindle organization Source: MGI
  • response to hypoxia Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming acidic coiled-coil-containing protein 3
Alternative name(s):
ARNT-interacting protein
Gene namesi
Name:Tacc3
Synonyms:Aint
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1341163. Tacc3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity

  • Note: In complex with CKAP5 localized to microtuble plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles.By similarity

GO - Cellular componenti

  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001799912 – 631Transforming acidic coiled-coil-containing protein 3Add BLAST630

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Modified residuei71PhosphoserineBy similarity1
Modified residuei347Phosphoserine; by AURKABy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JJ11.
PaxDbiQ9JJ11.
PRIDEiQ9JJ11.

PTM databases

iPTMnetiQ9JJ11.
PhosphoSitePlusiQ9JJ11.

Expressioni

Tissue specificityi

Embryonically expressed.

Developmental stagei

At 9 dpc, the expression is strong in the neuroepithelium of neural tube and in placenta. At 13 dpc, the expression is still observed in neuroepithelium. Furthermore, strong expression is seen in lung, kidney, intestines, thymus and liver, and a moderate signal is detected in the cartilage primordium of developing ribs, tooth and eye. By 17 dpc, the tissue distribution changes so that no signal is detected in the liver and the signal has diminished in other organs. It is observed for the first time in the salivary gland, thyroid gland and brown fat and was strong in the thymus, eye, olfactory epithelium and central nervous system. At 1.5 days after birth, the expression is still strong in thymus, but weaker and more limited in brain.

Interactioni

Subunit structurei

Interacts with GCN5L2 and PCAF (By similarity). The coiled coil C-terminal region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (PubMed:11025203). Interacts with CCDC100/CEP120 (PubMed:17920017). Interacts with CKAP5 independently of clathrin. Interacts with CKAP5 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; TACC3 (phosphorylated at Ser-347 by AURKA) and CLTC are proposed to form a composite microtubule interaction surface (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Zfpm1O356157EBI-2553611,EBI-4394596

GO - Molecular functioni

  • protein domain specific binding Source: MGI

Protein-protein interaction databases

IntActiQ9JJ11. 3 interactors.
STRINGi10090.ENSMUSP00000078491.

Structurei

Secondary structure

1631
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi610 – 623Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LPZX-ray3.15C/D610-631[»]
4PKYX-ray3.20B/C/E/F585-631[»]
ProteinModelPortaliQ9JJ11.
SMRiQ9JJ11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni311 – 366Necessary but not sufficient for spindle localizationBy similarityAdd BLAST56
Regioni384 – 631Necessary but not sufficient for spindle localizationBy similarityAdd BLAST248

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili431 – 630Sequence analysisAdd BLAST200

Sequence similaritiesi

Belongs to the TACC family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IIVK. Eukaryota.
ENOG410YMFS. LUCA.
HOGENOMiHOG000294059.
HOVERGENiHBG098556.
InParanoidiQ9JJ11.
PhylomeDBiQ9JJ11.

Family and domain databases

InterProiIPR007707. TACC.
[Graphical view]
PfamiPF05010. TACC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JJ11-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLHVLNDEN VPNEKSSQCR DFQFLPPELT GRSSVLCLSQ KENVPPQSQA
60 70 80 90 100
KATNVTFQTP PRDPQTHRIL SPNMTNKREA PFGLQNDHCV FLQKENQRPL
110 120 130 140 150
APVDDAPVVQ MAAEILRAEG ELQEGILTSS SLSASTSLLD SELVTPPIEP
160 170 180 190 200
VLEPSHQGLE PVLESELVTP PVEPVLEPSH QELEPVLESE LVTPPIEPVL
210 220 230 240 250
EPSHQGLEPV LDSELVTPPI EPVLEPSHQG LEPVLESELV TPPIEPVLEP
260 270 280 290 300
SHQGLEPVLD SELVTPPIEP VLEPSHQGLE PVLDSELVTP PIEPLLEPSH
310 320 330 340 350
QGLEPVVDLK EESFRDPSEV LGTGAEVDYL EQFGTSSFKE SAWRKQSLYV
360 370 380 390 400
KFDPLLKDSP LRPMPVAPIT NSTQDTEEES GSGKPTEAEL VNLDFLGDLD
410 420 430 440 450
VPVSAPTPVW SLEPRGLLPA EPIVDVLKYS QKDLDAVVNV MQQENLELKS
460 470 480 490 500
KYEDLNTKYL EMGKSVDEFE KIAYKSLEEA EKQRELKEIA EDKIQKVLKE
510 520 530 540 550
RDQLNADLNS MEKSFSDLFK RFEKRKEVIE GYQKNEESLK KYVGECIVKI
560 570 580 590 600
EKEGQRYQAL KIHAEEKLRL ANEEIAQVHS KAQAEVLALQ ASLRKAQMQN
610 620 630
HSLEMTLEQK TKEIDELTRI CDDLISKMEK I
Length:631
Mass (Da):70,627
Last modified:October 1, 2000 - v1
Checksum:i92D6324D3890E9CB
GO
Isoform 2 (identifier: Q9JJ11-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-277: Missing.
     337-337: S → SSHLSNSQ

Show »
Length:542
Mass (Da):60,969
Checksum:i1A082AE35C996230
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171P → S (PubMed:10366448).Curated1
Sequence conflicti407 – 411TPVWS → PLCV in AAD25963 (PubMed:10366448).Curated5

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006370182 – 277Missing in isoform 2. 1 PublicationAdd BLAST96
Alternative sequenceiVSP_006371337S → SSHLSNSQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156934 mRNA. Translation: AAF85763.1.
AF093542 mRNA. Translation: AAD25963.1.
UniGeneiMm.379024.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156934 mRNA. Translation: AAF85763.1.
AF093542 mRNA. Translation: AAD25963.1.
UniGeneiMm.379024.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LPZX-ray3.15C/D610-631[»]
4PKYX-ray3.20B/C/E/F585-631[»]
ProteinModelPortaliQ9JJ11.
SMRiQ9JJ11.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JJ11. 3 interactors.
STRINGi10090.ENSMUSP00000078491.

PTM databases

iPTMnetiQ9JJ11.
PhosphoSitePlusiQ9JJ11.

Proteomic databases

EPDiQ9JJ11.
PaxDbiQ9JJ11.
PRIDEiQ9JJ11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1341163. Tacc3.

Phylogenomic databases

eggNOGiENOG410IIVK. Eukaryota.
ENOG410YMFS. LUCA.
HOGENOMiHOG000294059.
HOVERGENiHBG098556.
InParanoidiQ9JJ11.
PhylomeDBiQ9JJ11.

Miscellaneous databases

PROiQ9JJ11.
SOURCEiSearch...

Family and domain databases

InterProiIPR007707. TACC.
[Graphical view]
PfamiPF05010. TACC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTACC3_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ11
Secondary accession number(s): Q9WVK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.