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Protein

Sodium-dependent phosphate transport protein 2B

Gene

Slc34a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in actively transporting phosphate into cells via Na+ cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: RGD
  • sodium-dependent phosphate transmembrane transporter activity Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • aging Source: RGD
  • phosphate ion transport Source: RGD
  • response to estradiol Source: RGD
  • response to fructose Source: RGD
  • sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent phosphate transport protein 2B
Short name:
Sodium-phosphate transport protein 2B
Alternative name(s):
Na(+)-dependent phosphate cotransporter 2B
Sodium/phosphate cotransporter 2B
Short name:
Na(+)/Pi cotransporter 2B
Short name:
NaPi-2b
Solute carrier family 34 member 2
Short name:
rNaPi IIb
Gene namesi
Name:Slc34a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620889. Slc34a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9090CytoplasmicSequence analysisAdd
BLAST
Transmembranei91 – 11121Helical; Name=M1Sequence analysisAdd
BLAST
Topological domaini112 – 13625ExtracellularSequence analysisAdd
BLAST
Transmembranei137 – 15721Helical; Name=M2Sequence analysisAdd
BLAST
Topological domaini158 – 21356CytoplasmicSequence analysisAdd
BLAST
Transmembranei214 – 23421Helical; Name=M3Sequence analysisAdd
BLAST
Topological domaini235 – 363129ExtracellularSequence analysisAdd
BLAST
Transmembranei364 – 38421Helical; Name=M4Sequence analysisAdd
BLAST
Topological domaini385 – 40824CytoplasmicSequence analysisAdd
BLAST
Transmembranei409 – 42921Helical; Name=M5Sequence analysisAdd
BLAST
Topological domaini430 – 48657ExtracellularSequence analysisAdd
BLAST
Transmembranei487 – 50721Helical; Name=M6Sequence analysisAdd
BLAST
Topological domaini508 – 52619CytoplasmicSequence analysisAdd
BLAST
Transmembranei527 – 54721Helical; Name=M7Sequence analysisAdd
BLAST
Topological domaini548 – 5514ExtracellularSequence analysis
Transmembranei552 – 57221Helical; Name=M8Sequence analysisAdd
BLAST
Topological domaini573 – 695123CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • brush border membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • microvillus membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695Sodium-dependent phosphate transport protein 2BPRO_0000068616Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence analysis
Disulfide bondi303 ↔ 350By similarity
Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence analysis
Glycosylationi321 – 3211N-linked (GlcNAc...)Sequence analysis
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JJ09.
PRIDEiQ9JJ09.

PTM databases

iPTMnetiQ9JJ09.

Expressioni

Tissue specificityi

Highly expressed in the lung, in type II alveolar cells. Moderately expressed in kidney followed by small intestine.1 Publication

Developmental stagei

Appears on embryonic day 16.5 and is expressed thereafter.1 Publication

Inductioni

Up-regulated by estrogen.1 Publication

Gene expression databases

GenevisibleiQ9JJ09. RN.

Interactioni

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050889.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi465 – 4684Poly-Thr
Compositional biasi613 – 64432Cys-richAdd
BLAST
Compositional biasi648 – 6536Poly-Glu

Sequence similaritiesi

Belongs to the SLC34A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
HOGENOMiHOG000006550.
HOVERGENiHBG006527.
InParanoidiQ9JJ09.
KOiK14683.
OMAiKTECTAL.
OrthoDBiEOG72ZCDP.
PhylomeDBiQ9JJ09.
TreeFamiTF313981.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029852. Na/Pi_transpt_2B.
[Graphical view]
PANTHERiPTHR10010:SF23. PTHR10010:SF23. 2 hits.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JJ09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPWPELENA HPNPNKFIEG ASGPQSSIPD KDKGTSKTND SGTPVAKIEL
60 70 80 90 100
LPSYSALVLI EEPPEGNDPW DLPELQDNGI KWSERDSKGK ILCIFQGIGK
110 120 130 140 150
FILLLGFLYL FVCSLDVLSS AFQLVGGKMA GQFFSNNSIM SNPVAGLVIG
160 170 180 190 200
VLVTVMVQSS STSSSIIVSM VASSLLSVRA AIPIIMGANI GTSITNTIVA
210 220 230 240 250
LMQAGDRNEF RRAFAGATVH DFFNWLSVLV LLPLEAATHY LEKLTNLVLE
260 270 280 290 300
TFSFQNGEDA PDILKVITDP FTKLIIQLDK KVIQQIAMGD SEAQNKSLIK
310 320 330 340 350
IWCKTISNVI EENVTVPSPD NCTSPSYCWT DGIQTWTIQN VTEKENIAKC
360 370 380 390 400
QHIFVNFSLP DLAVGIILLT VSLLILCGCL IMIVKLLGSV LRGQVATVIK
410 420 430 440 450
KTLNTDFPFP FAWLTGYLAI LVGAGMTFIV QSSSVFTSAM TPLIGIGVIS
460 470 480 490 500
IERAYPLTLG SNIGTTTTAI LAALASPGNT LRSSLQIALC HFFFNISGIL
510 520 530 540 550
LWYPIPFTRL PIRLAKGLGN ISAKYRWFAV FYLIFFFLLT PLTVFGLSLA
560 570 580 590 600
GWPVLVGVGV PIILLILLVL CLRMLQARCP RILPLKLRDW NFLPLWMHSL
610 620 630 640 650
KPWDNIISLA TSCFQRRCCC CCRVCCRVCC MVCGCKCCRC SKCCKNLEEE
660 670 680 690
EKEQDVPVKA SGGFDNTAMS KECQDEGKGQ VEVLGMKALS NTTVF
Length:695
Mass (Da):75,992
Last modified:October 1, 2000 - v1
Checksum:i3FAFB827527E0061
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157026 mRNA. Translation: AAF76291.1.
BC070898 mRNA. Translation: AAH70898.1.
AF247725 mRNA. Translation: AAL55704.1.
RefSeqiNP_445832.1. NM_053380.2.
UniGeneiRn.16933.

Genome annotation databases

GeneIDi84395.
KEGGirno:84395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157026 mRNA. Translation: AAF76291.1.
BC070898 mRNA. Translation: AAH70898.1.
AF247725 mRNA. Translation: AAL55704.1.
RefSeqiNP_445832.1. NM_053380.2.
UniGeneiRn.16933.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050889.

PTM databases

iPTMnetiQ9JJ09.

Proteomic databases

PaxDbiQ9JJ09.
PRIDEiQ9JJ09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84395.
KEGGirno:84395.

Organism-specific databases

CTDi10568.
RGDi620889. Slc34a2.

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
HOGENOMiHOG000006550.
HOVERGENiHBG006527.
InParanoidiQ9JJ09.
KOiK14683.
OMAiKTECTAL.
OrthoDBiEOG72ZCDP.
PhylomeDBiQ9JJ09.
TreeFamiTF313981.

Miscellaneous databases

PROiQ9JJ09.

Gene expression databases

GenevisibleiQ9JJ09. RN.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029852. Na/Pi_transpt_2B.
[Graphical view]
PANTHERiPTHR10010:SF23. PTHR10010:SF23. 2 hits.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and localization of type IIb Na/Pi cotransporter in the developing rat lung."
    Hashimoto M., Wang D.-Y., Kamo T., Zhu Y., Tsujiuchi T., Konishi Y., Tanaka M., Sugimura H.
    Am. J. Pathol. 157:21-27(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Wistar.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Lung.
  3. "Molecular cloning and functional characterization of an intestinal sodium-phosphate transporter gene promoter and its gene structure."
    Xu H., Bai L., Collins J.F., Ghishan F.K.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 215-466.
    Tissue: Intestine.
  4. "Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen."
    Xu H., Uno J.K., Inouye M., Xu L., Drees J.B., Collins J.F., Ghishan F.K.
    Am. J. Physiol. 285:G1317-G1324(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: Sprague-Dawley.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNPT2B_RAT
AccessioniPrimary (citable) accession number: Q9JJ09
Secondary accession number(s): Q8VI55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.