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Protein

Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1

Gene

C1galt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.2 Publications

Catalytic activityi

UDP-alpha-D-galactose + glycoprotein N-acetyl-D-galactosamine = UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine.

Cofactori

Mg2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: MGI
  • intestinal epithelial cell development Source: MGI
  • kidney development Source: MGI
  • O-glycan processing, core 1 Source: MGI
  • protein O-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.122. 3474.
ReactomeiR-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (EC:2.4.1.122)
Alternative name(s):
Core 1 O-glycan T-synthase
Short name:
T-syn
Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1
Core 1 beta1,3-galactosyltransferase 1
Short name:
C1GalT1
Short name:
Core 1 beta3-Gal-T1
Gene namesi
Name:C1galt1
Synonyms:Plt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2151071. C1galt1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 8CytoplasmicSequence analysis7
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 363LumenalSequence analysisAdd BLAST334

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice develop brain hemorrhage that cause death at E14 during development. They express the nonsialylated Tn antigen and brains form a chaotic microvascular network with distorted capillary lumens and defective association of endothelial cells with pericytes and extracellular matrix.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi321Y → N in plt1; loss of function inducing thrombocytopenia and kidney disease. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002850652 – 363Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei235PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9JJ06.
MaxQBiQ9JJ06.
PaxDbiQ9JJ06.
PeptideAtlasiQ9JJ06.
PRIDEiQ9JJ06.

PTM databases

iPTMnetiQ9JJ06.
PhosphoSitePlusiQ9JJ06.

Expressioni

Tissue specificityi

Primarily expressed in endothelial, hematopoietic and epithelial cells during development.1 Publication

Gene expression databases

BgeeiENSMUSG00000042460.
GenevisibleiQ9JJ06. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with the C1GALT1C1 chaperone; required for galactosyltransferase activity (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047931.

Structurei

3D structure databases

ProteinModelPortaliQ9JJ06.
SMRiQ9JJ06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2246. Eukaryota.
ENOG410YRJG. LUCA.
GeneTreeiENSGT00530000063264.
HOGENOMiHOG000021316.
HOVERGENiHBG097900.
InParanoidiQ9JJ06.
KOiK00731.
OMAiQYVHEHY.
OrthoDBiEOG091G0BLV.
PhylomeDBiQ9JJ06.
TreeFamiTF317293.

Family and domain databases

InterProiIPR026842. C1GALT1.
IPR003378. Fringe-like.
[Graphical view]
PANTHERiPTHR23033:SF13. PTHR23033:SF13. 1 hit.
PfamiPF02434. Fringe. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JJ06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKSWLNFL VFLCGSAIGF FLCSQLLSIL LREEAAIQPN MLHNDPHARH
60 70 80 90 100
SDDNGHSHLK GQMNFNADSS QHKDENIDVA ENLYQKVKIL CWVMTSPQNL
110 120 130 140 150
EKKAKHVKAT WAQRCNKVLF MSSEENQDFP TVGLKTKEGR EQLYWKTIKA
160 170 180 190 200
FQYVHDHYLE DADWFMKADD DTYVIVDNLR WLLSKYNPEQ PIYFGRRFKP
210 220 230 240 250
YVKQGYMSGG AGYVLSKEAL RRFVNAFKTE KCTHSSSIED LALGRCMEII
260 270 280 290 300
NVEAGDSRDT IGKETFHPFV PEHHLIKGYL PKTFWYWNYN YYPPIEGPGC
310 320 330 340 350
CSDIAVSFHY VDGTTMYELE YLVYHLRPYG YLYRYQPALP ENILKEINQV
360
NRKEDTKIKL GNP
Length:363
Mass (Da):42,304
Last modified:October 1, 2000 - v1
Checksum:i3279FBC5AE660EB4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187N → D in AAH64767 (PubMed:15489334).Curated1
Sequence conflicti325H → R in AAH64767 (PubMed:15489334).Curated1
Sequence conflicti331Y → C in AAH64767 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157962 mRNA. Translation: AAF81982.1.
BC025899 mRNA. Translation: AAH25899.1.
BC064767 mRNA. Translation: AAH64767.1.
CCDSiCCDS19908.1.
RefSeqiNP_443719.3. NM_052993.3.
UniGeneiMm.102752.
Mm.482425.

Genome annotation databases

EnsembliENSMUST00000040159; ENSMUSP00000047931; ENSMUSG00000042460.
GeneIDi94192.
KEGGimmu:94192.
UCSCiuc009axg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157962 mRNA. Translation: AAF81982.1.
BC025899 mRNA. Translation: AAH25899.1.
BC064767 mRNA. Translation: AAH64767.1.
CCDSiCCDS19908.1.
RefSeqiNP_443719.3. NM_052993.3.
UniGeneiMm.102752.
Mm.482425.

3D structure databases

ProteinModelPortaliQ9JJ06.
SMRiQ9JJ06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047931.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

iPTMnetiQ9JJ06.
PhosphoSitePlusiQ9JJ06.

Proteomic databases

EPDiQ9JJ06.
MaxQBiQ9JJ06.
PaxDbiQ9JJ06.
PeptideAtlasiQ9JJ06.
PRIDEiQ9JJ06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040159; ENSMUSP00000047931; ENSMUSG00000042460.
GeneIDi94192.
KEGGimmu:94192.
UCSCiuc009axg.2. mouse.

Organism-specific databases

CTDi56913.
MGIiMGI:2151071. C1galt1.

Phylogenomic databases

eggNOGiKOG2246. Eukaryota.
ENOG410YRJG. LUCA.
GeneTreeiENSGT00530000063264.
HOGENOMiHOG000021316.
HOVERGENiHBG097900.
InParanoidiQ9JJ06.
KOiK00731.
OMAiQYVHEHY.
OrthoDBiEOG091G0BLV.
PhylomeDBiQ9JJ06.
TreeFamiTF317293.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.122. 3474.
ReactomeiR-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSiC1galt1. mouse.
PROiQ9JJ06.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042460.
GenevisibleiQ9JJ06. MM.

Family and domain databases

InterProiIPR026842. C1GALT1.
IPR003378. Fringe-like.
[Graphical view]
PANTHERiPTHR23033:SF13. PTHR23033:SF13. 1 hit.
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC1GLT_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ06
Secondary accession number(s): Q6P218, Q8R0Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.