Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9JJ04 (B4GT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 4
Short name=Beta-1,4-GalTase 4
Short name=Beta4Gal-T4
Short name=b4Gal-T4
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Including the following 2 domains:

  1. N-acetyllactosamine synthase
    EC=2.4.1.90
    Alternative name(s):
    Nal synthase
  2. Lactotriaosylceramide beta-1,4-galactosyltransferase
    EC=2.4.1.275
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene names
Name:B4galt4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Catalytic activity

UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

UDP-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)--D-glucosyl-(1<->1)-ceramide.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Sequence caution

The sequence BAC32433.1 differs from that shown. Reason: Frameshift at position 321.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Beta-1,4-galactosyltransferase 4
PRO_0000080543

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3826Helical; Signal-anchor for type II membrane protein; Potential
Topological domain39 – 344306Lumenal Potential

Sites

Metal binding1961Manganese By similarity
Metal binding2891Manganese By similarity

Amino acid modifications

Glycosylation2201N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential
Disulfide bond77 ↔ 118 By similarity
Disulfide bond189 ↔ 208 By similarity

Experimental info

Sequence conflict2381L → F in AAF22222. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JJ04 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 74FA110191A4BCC8

FASTA34439,700
        10         20         30         40         50         60 
MGCNPPYHLS YRLRLLLLFT LCLTVVGWAT SNYFVGAIQV IPKAKDFMAS FHKVIHLGNE 

        70         80         90        100        110        120 
ETLGHDGATK KPELANCPSV SPNLRGQSKL VFKPDLTLEE IEAENPKVSR GRYHPEECKA 

       130        140        150        160        170        180 
LQRVAILIPH RNREKHLIYL LEHLHPFLQR QQLDYGIYII HQTGSKKFNR AKLLNVGYLE 

       190        200        210        220        230        240 
ALKEENWDCF VFHDVDLVPE NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR 

       250        260        270        280        290        300 
EQFLKVNGFS NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNMG 

       310        320        330        340 
RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA

« Hide

References

« Hide 'large scale' references
[1]"The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
Glycobiology 8:517-526(1998) [PubMed: 9597550] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of mouse beta-1,4-galactosyltransferase 4."
Schwientek T., Clausen H.
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Corpora quadrigemina, Hippocampus, Kidney and Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon and Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF142672 mRNA. Translation: AAF22222.1.
AF158746 mRNA. Translation: AAF80363.1.
AK041692 mRNA. Translation: BAC31035.1.
AK045602 mRNA. Translation: BAC32433.1. Frameshift.
AK050005 mRNA. Translation: BAC34028.1.
AK052013 mRNA. Translation: BAC34832.1.
AK053603 mRNA. Translation: BAC35443.1.
AK085368 mRNA. Translation: BAC39433.1.
BC013492 mRNA. Translation: AAH13492.1.
BC031115 mRNA. Translation: AAH31115.1.
IPIIPI00224047.
RefSeqNP_062778.2. NM_019804.3.
UniGeneMm.182377.

3D structure databases

ProteinModelPortalQ9JJ04.
SMRQ9JJ04. Positions 73-344.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9JJ04.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Proteomic databases

PRIDEQ9JJ04.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023482; ENSMUSP00000023482; ENSMUSG00000022793.
ENSMUST00000114712; ENSMUSP00000110360; ENSMUSG00000022793.
GeneID56375.
KEGGmmu:56375.

Organism-specific databases

CTD8702.
MGIMGI:1928387. B4galt4.

Phylogenomic databases

GeneTreeENSGT00550000074204.
HOGENOMHBG717407.
HOVERGENHBG058334.
InParanoidQ9JJ04.
OMATDGLSSC.
OrthoDBEOG40P474.

Gene expression databases

ArrayExpressQ9JJ04.
BgeeQ9JJ04.
GenevestigatorQ9JJ04.
GermOnlineENSMUSG00000022793. Mus musculus.

Family and domain databases

InterProIPR003859. Galactosyl_T_2_met.
[Graphical view]
KOK07969.
PANTHERPTHR19300. Galactosyl_T_2. 1 hit.
PfamPF02709. Galactosyl_T_2. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
ProtoNetSearch...

Other

NextBio312440.
SOURCESearch...

Entry information

Entry nameB4GT4_MOUSE
AccessionPrimary (citable) accession number: Q9JJ04
Secondary accession number(s): Q8BR54, Q9QY12
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents