Reviewed, UniProtKB/Swiss-Prot Q9JJ04 (B4GT4_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 4
      Short name=Beta-1,4-GalTase 4
      Short name=Beta4Gal-T4
      Short name=b4Gal-T4
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
Including the following 2 domains:
    1- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    2- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:

B4galt4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.
Catalytic activity	UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.
Sequence caution	The sequence BAC32433.1 differs from that shown. Reason: Frameshift at position 321.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Beta-1,4-galactosyltransferase 4		PRO_0000080543
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 38	26	Signal-anchor for type II membrane protein Potential
	Topological domain	39 – 344	306	Lumenal Potential
Sites
	Metal binding	196	1	Manganese By similarity
	Metal binding	289	1	Manganese By similarity
Amino acid modifications
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Disulfide bond	77 ↔ 118		By similarity
	Disulfide bond	189 ↔ 208		By similarity
Experimental info
	Sequence conflict	238	1	L → F in AAF22222. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9JJ04-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 74FA110191A4BCC8	FASTA	344	39,700
	10 20 30 40 50 60 MGCNPPYHLS YRLRLLLLFT LCLTVVGWAT SNYFVGAIQV IPKAKDFMAS FHKVIHLGNE 70 80 90 100 110 120 ETLGHDGATK KPELANCPSV SPNLRGQSKL VFKPDLTLEE IEAENPKVSR GRYHPEECKA 130 140 150 160 170 180 LQRVAILIPH RNREKHLIYL LEHLHPFLQR QQLDYGIYII HQTGSKKFNR AKLLNVGYLE 190 200 210 220 230 240 ALKEENWDCF VFHDVDLVPE NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR 250 260 270 280 290 300 EQFLKVNGFS NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNMG 310 320 330 340 RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expanding beta 4-galactosyltransferase gene family: messages from the databanks." Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L. Glycobiology 8:517-526(1998) [PubMed: 9597550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of mouse beta-1,4-galactosyltransferase 4." Schwientek T., Clausen H. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Corpora quadrigemina, Hippocampus, Kidney and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon and Mammary tumor.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b4GalT4

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF142672 mRNA. Translation: AAF22222.1. AF158746 mRNA. Translation: AAF80363.1. AK041692 mRNA. Translation: BAC31035.1. AK045602 mRNA. Translation: BAC32433.1. Frameshift. AK050005 mRNA. Translation: BAC34028.1. AK052013 mRNA. Translation: BAC34832.1. AK053603 mRNA. Translation: BAC35443.1. AK085368 mRNA. Translation: BAC39433.1. BC013492 mRNA. Translation: AAH13492.1. BC031115 mRNA. Translation: AAH31115.1.
IPI	IPI00224047.
RefSeq	NP_062778.2.
UniGene	Mm.182377
3D structure databases
SMR	Q9JJ04. Positions 74-344.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9JJ04.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	Q9JJ04.
Genome annotation databases
Ensembl	ENSMUST00000023482; ENSMUSP00000023482; ENSMUSG00000022793; Mus musculus. [Genome view] ENSMUST00000114712; ENSMUSP00000110360; ENSMUSG00000022793; Mus musculus. [Genome view]
GeneID	56375.
KEGG	mmu:56375.
UCSC	uc007zfi.1. mouse.
Organism-specific databases
CTD	56375.
MGI	MGI:1928387. B4galt4.
Phylogenomic databases
HOGENOM	HBG717407.
HOVERGEN	Q9JJ04.
InParanoid	Q9JJ04.
OMA	VWRTDGL.
OrthoDB	EOG9HHRNS.
Enzyme and pathway databases
BRENDA	2.4.1.90. 244.
Gene expression databases
ArrayExpress	Q9JJ04.
Bgee	Q9JJ04.
Genevestigator	Q9JJ04.
GermOnline	ENSMUSG00000022793. Mus musculus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	312440.
SOURCE	Search...

Entry information

Entry name

B4GT4_MOUSE

Accession

Primary (citable) accession number: Q9JJ04
Secondary accession number(s): Q8BR54, Q9QY12

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)